BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126009.1 - phase: 0
(326 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86718 similar to GP|15010742|gb|AAK74030.1 At1g54320/F20D21_50... 416 e-117
AJ388904 similar to GP|21618040|gb unknown {Arabidopsis thaliana... 200 5e-52
TC92263 similar to GP|15294236|gb|AAK95295.1 AT3g12740/MBK21_10 ... 198 3e-51
TC83729 similar to PIR|T39676|T39676 probable yeast cell divisio... 108 3e-24
BG588800 33 0.15
AL369576 similar to SP|O24304|PFTA Protein farnesyltransferase a... 32 0.44
BG583133 similar to GP|15010742|gb| At1g54320/F20D21_50 {Arabido... 30 0.97
CB892400 29 2.8
TC83126 similar to GP|15529155|gb|AAK97672.1 AT3g30390/T6J22_16 ... 29 2.8
BI269224 weakly similar to GP|11862972|db hypothetical protein {... 28 3.7
TC93440 similar to GP|14587312|dbj|BAB61223. contains EST AU0573... 27 8.2
TC86092 similar to GP|13940209|emb|CAC37922. fructan 1-exohydrol... 27 8.2
TC86090 similar to GP|13940211|emb|CAC37923. fructan 1-exohydrol... 27 8.2
AW287942 weakly similar to GP|14331118|em ABC1 protein {Nicotian... 27 8.2
>TC86718 similar to GP|15010742|gb|AAK74030.1 At1g54320/F20D21_50
{Arabidopsis thaliana}, partial (90%)
Length = 1572
Score = 416 bits (1068), Expect = e-117
Identities = 204/318 (64%), Positives = 243/318 (76%)
Frame = +1
Query: 4 SRFSQQELHAWQPILTPGWVIAIFTFIGLVFIPIGVASLFASEQVVEVPLRYDDQCLPSL 63
S+F+QQEL A +PILTP VI+ F + +VFIPIGVASL AS +VVE+ Y+ CLP
Sbjct: 298 SKFTQQELPACKPILTPRAVISAFLLVSVVFIPIGVASLMASRKVVEIVYGYESDCLPDG 477
Query: 64 YKDDAMTYIKGNRISKTCTKKLTVKSKMKAPIYVYYQLSNFYQNHRHYVKSRDHKQLRSK 123
KD+ + YI+ KTC L V MK+PIYVYYQL NFYQNHR YVKSR +QLRS+
Sbjct: 478 TKDE-IAYIQSPETDKTCNITLNVTKHMKSPIYVYYQLDNFYQNHRRYVKSRSDEQLRSR 654
Query: 124 ADENDVGKCFPEDYTANGYLPVVPCGLAAWSLFNDTYRFSNNNKDLVINKKNIAWKSDQK 183
EN V C PED TAN +VPCGL AWSLFNDTY FS NN +L +NKK+I+WKSD++
Sbjct: 655 KGENSVSACKPED-TANNR-SIVPCGLIAWSLFNDTYSFSVNNTNLTVNKKDISWKSDRE 828
Query: 184 AKFGSDVYPKNFQTGSLIGGARLNESIPLSEQEDLIVWMRTAALPTFRKLYGKIEVDLEA 243
KFG DV+PKNFQ GS+IGGA+LNESIPL+EQEDLIVWMRTAALPTFRKLYGKIEVDL
Sbjct: 829 HKFGKDVFPKNFQNGSIIGGAQLNESIPLNEQEDLIVWMRTAALPTFRKLYGKIEVDLNQ 1008
Query: 244 NDEITVVIENNYNTYQFGGTKSVILSTTTWIGGKNDFLGIAYILIGGLSLVYSLVFLLMY 303
D I V ++NNYNTY F G K ++LSTT+W+GGKNDFLGIAY+ +GGL SL F ++Y
Sbjct: 1009GDLIDVTLKNNYNTYSFNGKKKLVLSTTSWLGGKNDFLGIAYLTVGGLCFFLSLAFTIVY 1188
Query: 304 LMKPRPLGDPRYLTWNKN 321
+KPR LGDP YL+WN+N
Sbjct: 1189FVKPRQLGDPSYLSWNRN 1242
>AJ388904 similar to GP|21618040|gb unknown {Arabidopsis thaliana}, partial
(35%)
Length = 561
Score = 200 bits (509), Expect = 5e-52
Identities = 90/121 (74%), Positives = 109/121 (89%)
Frame = +2
Query: 201 IGGARLNESIPLSEQEDLIVWMRTAALPTFRKLYGKIEVDLEANDEITVVIENNYNTYQF 260
IGGA+LNES+PLSEQEDLIVWMRTAALPTFRKLYGKIE DLE NDEI ++I+NNYNTY+F
Sbjct: 2 IGGAKLNESVPLSEQEDLIVWMRTAALPTFRKLYGKIESDLEVNDEIEILIQNNYNTYEF 181
Query: 261 GGTKSVILSTTTWIGGKNDFLGIAYILIGGLSLVYSLVFLLMYLMKPRPLGDPRYLTWNK 320
G K ++LSTTTWIGGKN FLG+AY+ +GGLSL ++ F+L+Y++KPRPLGDP YL+WN+
Sbjct: 182 QGRKKLVLSTTTWIGGKNRFLGVAYLCVGGLSLFCAIGFILLYVIKPRPLGDPSYLSWNR 361
Query: 321 N 321
N
Sbjct: 362 N 364
>TC92263 similar to GP|15294236|gb|AAK95295.1 AT3g12740/MBK21_10
{Arabidopsis thaliana}, partial (50%)
Length = 754
Score = 198 bits (503), Expect = 3e-51
Identities = 101/179 (56%), Positives = 128/179 (71%)
Frame = +3
Query: 4 SRFSQQELHAWQPILTPGWVIAIFTFIGLVFIPIGVASLFASEQVVEVPLRYDDQCLPSL 63
S+F+QQEL A +PILTP VI+ F + +VFIPIGVASL AS VVE+ RY+ C+PS
Sbjct: 222 SKFTQQELPACKPILTPQAVISAFLIVTIVFIPIGVASLIASRDVVEIVDRYEAACVPSN 401
Query: 64 YKDDAMTYIKGNRISKTCTKKLTVKSKMKAPIYVYYQLSNFYQNHRHYVKSRDHKQLRSK 123
+ D+ + YI+ KTCT+ L V+ +MK+PIYVYYQL NFYQNHR YVKSR+ +QLR
Sbjct: 402 WTDN-VAYIQSTA-DKTCTRTLHVEKRMKSPIYVYYQLDNFYQNHRRYVKSRNDEQLRDA 575
Query: 124 ADENDVGKCFPEDYTANGYLPVVPCGLAAWSLFNDTYRFSNNNKDLVINKKNIAWKSDQ 182
N C PE NG +P+VPCGL AWS+FNDTY FS NN ++ +NKK I+WKSD+
Sbjct: 576 GKANSTSGCDPE-RDVNG-VPIVPCGLIAWSMFNDTYSFSRNNNNVTVNKKEISWKSDR 746
>TC83729 similar to PIR|T39676|T39676 probable yeast cell division cycle
CDC50 homolog - fission yeast (Schizosaccharomyces
pombe), partial (19%)
Length = 557
Score = 108 bits (270), Expect = 3e-24
Identities = 52/112 (46%), Positives = 73/112 (64%)
Frame = +3
Query: 212 LSEQEDLIVWMRTAALPTFRKLYGKIEVDLEANDEITVVIENNYNTYQFGGTKSVILSTT 271
++E E VWMRTA LP FRKL+GK E D I+ + ++GGTKS+++ST
Sbjct: 75 IAEDEHFQVWMRTAGLPDFRKLWGKNEKDTMKMGRYQFEIQYLFPVTKYGGTKSLVISTV 254
Query: 272 TWIGGKNDFLGIAYILIGGLSLVYSLVFLLMYLMKPRPLGDPRYLTWNKNSK 323
+++GGKN FLG AYI++G + +V +F L +L KPR LGD YL+WN N +
Sbjct: 255 SFLGGKNSFLGWAYIVVGVICVVLGCLFTLRHLYKPRKLGDHTYLSWNNNGQ 410
>BG588800
Length = 809
Score = 33.1 bits (74), Expect = 0.15
Identities = 20/73 (27%), Positives = 36/73 (48%), Gaps = 12/73 (16%)
Frame = -2
Query: 67 DAMTYIKGNRISKTCTKKLTVKSKMKAPIYVYYQLSNFYQN------------HRHYVKS 114
+ M ++GN I + CT ++ ++ + K I V Q+++F + H+HY+K
Sbjct: 631 EQMNNLRGNNIFR-CTTRMVLERQRKHSIVVAEQINHFMKE*HL*MYHQMV*KHQHYLKL 455
Query: 115 RDHKQLRSKADEN 127
+ S ADEN
Sbjct: 454 QAESHQPSAADEN 416
>AL369576 similar to SP|O24304|PFTA Protein farnesyltransferase alpha
subunit (EC 2.5.1.-) (CAAX farnesyltransferase alpha
subunit), partial (39%)
Length = 505
Score = 31.6 bits (70), Expect = 0.44
Identities = 16/49 (32%), Positives = 24/49 (48%), Gaps = 1/49 (2%)
Frame = +1
Query: 42 LFASEQVVEVPLRYDD-QCLPSLYKDDAMTYIKGNRISKTCTKKLTVKS 89
L+ E ++ P + L LYKD+A Y+ ++S C K L KS
Sbjct: 34 LYTIEAIISYPENESSWRYLRGLYKDEATLYVNDTQVSSVCLKILRTKS 180
>BG583133 similar to GP|15010742|gb| At1g54320/F20D21_50 {Arabidopsis
thaliana}, partial (4%)
Length = 751
Score = 30.4 bits (67), Expect = 0.97
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = +1
Query: 302 MYLMKPRPLGDPRYLTWNKN 321
+Y+ R LGDP YL+WN+N
Sbjct: 667 LYVSSFRHLGDPSYLSWNRN 726
>CB892400
Length = 738
Score = 28.9 bits (63), Expect = 2.8
Identities = 19/59 (32%), Positives = 24/59 (40%), Gaps = 1/59 (1%)
Frame = -3
Query: 143 LPVVPCGLAAWSLFNDTYRFSNNNKDLV-INKKNIAWKSDQKAKFGSDVYPKNFQTGSL 200
LP VPCG S + NN + K+N D A FG+ KN GS+
Sbjct: 412 LPAVPCGDKVASSLQLLLKMDFNNSSFPPVTKRNSTDGRDDNASFGTHSILKNESWGSI 236
>TC83126 similar to GP|15529155|gb|AAK97672.1 AT3g30390/T6J22_16
{Arabidopsis thaliana}, partial (9%)
Length = 452
Score = 28.9 bits (63), Expect = 2.8
Identities = 24/72 (33%), Positives = 36/72 (49%), Gaps = 3/72 (4%)
Frame = +2
Query: 236 KIEVDLEANDEITVVIENNYNTYQFGGTKSVILSTTTWIGGKNDFLGIAYILIG---GLS 292
KIEV++E D+ + +NN + F G SV +TT IG L A ++G G++
Sbjct: 110 KIEVEIEHGDDGSNSNKNNESAASFAG--SVFNLSTTIIGAGIMALPAAMKVLGLTIGIA 283
Query: 293 LVYSLVFLLMYL 304
+ L F YL
Sbjct: 284 SIIFLAFAYSYL 319
>BI269224 weakly similar to GP|11862972|db hypothetical protein {Oryza sativa
(japonica cultivar-group)}, partial (26%)
Length = 537
Score = 28.5 bits (62), Expect = 3.7
Identities = 22/67 (32%), Positives = 35/67 (51%)
Frame = +2
Query: 236 KIEVDLEANDEITVVIENNYNTYQFGGTKSVILSTTTWIGGKNDFLGIAYILIGGLSLVY 295
KIEV++E D+ + +NN + F G SV +TT IG L A ++G +
Sbjct: 74 KIEVEIEHGDDGSNSNKNNESAASFSG--SVFNLSTTIIGAGIMALPAAMKVLGLTIGIA 247
Query: 296 SLVFLLM 302
S++FL +
Sbjct: 248 SIIFLAL 268
>TC93440 similar to GP|14587312|dbj|BAB61223. contains EST
AU057376(S21389)~similar to Arabidopsis thaliana
chromosome 5 F8F6.250~unknown, partial (9%)
Length = 738
Score = 27.3 bits (59), Expect = 8.2
Identities = 17/39 (43%), Positives = 19/39 (48%), Gaps = 7/39 (17%)
Frame = +3
Query: 213 SEQEDLIVW-------MRTAALPTFRKLYGKIEVDLEAN 244
SE DLI W MRTA+ TF +EVD E N
Sbjct: 282 SENVDLISWTKSGGPLMRTASANTFVDFLRNLEVDTELN 398
>TC86092 similar to GP|13940209|emb|CAC37922. fructan 1-exohydrolase IIa
{Cichorium intybus}, partial (18%)
Length = 729
Score = 27.3 bits (59), Expect = 8.2
Identities = 18/58 (31%), Positives = 27/58 (46%), Gaps = 8/58 (13%)
Frame = +1
Query: 272 TWIGGKNDFLGIAYILIGGLSLVYSLVFLLM--------YLMKPRPLGDPRYLTWNKN 321
T G + F G A I+ G ++Y L L+ YL P+ L DP+ + W K+
Sbjct: 346 TCAGETSCFSGSATIVPGEQPVIYMLYTGLINEKTHQVQYLAMPKDLSDPKLIEWIKH 519
>TC86090 similar to GP|13940211|emb|CAC37923. fructan 1-exohydrolase IIb
{Cichorium intybus}, partial (33%)
Length = 1357
Score = 27.3 bits (59), Expect = 8.2
Identities = 18/58 (31%), Positives = 27/58 (46%), Gaps = 8/58 (13%)
Frame = +3
Query: 272 TWIGGKNDFLGIAYILIGGLSLVYSLVFLLM--------YLMKPRPLGDPRYLTWNKN 321
T G + F G A I+ G ++Y L L+ YL P+ L DP+ + W K+
Sbjct: 333 TCAGETSCFSGSATIVPGEQPVIYMLYTGLINEKTHQVQYLAMPKDLSDPKLIEWIKH 506
>AW287942 weakly similar to GP|14331118|em ABC1 protein {Nicotiana
plumbaginifolia}, partial (5%)
Length = 511
Score = 27.3 bits (59), Expect = 8.2
Identities = 16/56 (28%), Positives = 30/56 (53%)
Frame = +1
Query: 244 NDEITVVIENNYNTYQFGGTKSVILSTTTWIGGKNDFLGIAYILIGGLSLVYSLVF 299
N +T +N +T G +SV + G + DFLG+ I++ S++++L+F
Sbjct: 88 NGLVTSQYGHNMDTLDNG--QSVEEFVRNYFGFEYDFLGVVAIVVVSFSVLFALIF 249
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.138 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,066,250
Number of Sequences: 36976
Number of extensions: 159823
Number of successful extensions: 875
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 866
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 871
length of query: 326
length of database: 9,014,727
effective HSP length: 96
effective length of query: 230
effective length of database: 5,465,031
effective search space: 1256957130
effective search space used: 1256957130
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC126009.1