BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC125478.7 - phase: 0
(357 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF633515 151 4e-37
TC83089 144 5e-35
BE943429 130 6e-31
BG453710 weakly similar to GP|21537057|gb| unknown {Arabidopsis ... 71 5e-19
BG646599 70 9e-14
TC82474 71 7e-13
BG454068 weakly similar to GP|22202804|dbj hypothetical protein~... 65 5e-11
AW693426 homologue to GP|23307580|dbj similar to serine/threonin... 44 7e-05
TC77808 similar to PIR|T03843|T03843 prohibitin - common tobacco... 30 1.4
TC81072 similar to PIR|T05401|T05401 hypothetical protein F10M6.... 28 4.2
AW299072 weakly similar to GP|9796478|gb|A serine threonine kina... 28 7.1
>BF633515
Length = 249
Score = 151 bits (381), Expect = 4e-37
Identities = 71/82 (86%), Positives = 76/82 (92%)
Frame = +2
Query: 10 LDDVACLTHLSIEGRMLAHGKKMPKHEGAALLMTYLGVAQHEAEKICNQEYGGYISYPRL 69
LDDVACLTHL IEGRML+HGKKMPKHEGAALLM +LGV+Q EAEKIC QEYGGYISYPRL
Sbjct: 2 LDDVACLTHLPIEGRMLSHGKKMPKHEGAALLMRHLGVSQQEAEKICGQEYGGYISYPRL 181
Query: 70 RDFYTSYLGRANVLAGTEDPEE 91
RDFYT+YLGRAN+LA TEDP E
Sbjct: 182RDFYTTYLGRANLLASTEDPVE 247
>TC83089
Length = 887
Score = 144 bits (363), Expect = 5e-35
Identities = 72/116 (62%), Positives = 88/116 (75%), Gaps = 1/116 (0%)
Frame = +3
Query: 69 LRDFYTSYLGRANVLAGTEDPEEVEELERVRTYCVRCYLLYLVGCLLFGDRSNKRIELIY 128
LR++Y YL A L+ ++ + +ELE++RT CV+ YLLYLVGCLLFGD+SNKRIEL+Y
Sbjct: 264 LREYYEGYLDSATRLSDPQNLGDPQELEKIRTTCVKWYLLYLVGCLLFGDKSNKRIELVY 443
Query: 129 LTTMADGYAGMRNYSWGAMTLAYLYGELADACRLGHRALGGSVTLLTA-WFLAHFP 183
LTTM DGYAGMRN+SWG MTLAYLY L++ G AL SVTL+TA WFL H P
Sbjct: 444 LTTMEDGYAGMRNHSWGGMTLAYLYHCLSEVSLPGGGALERSVTLVTAGWFLCHLP 611
Score = 66.6 bits (161), Expect = 1e-11
Identities = 38/96 (39%), Positives = 51/96 (52%), Gaps = 3/96 (3%)
Frame = +3
Query: 251 WIMCGVRRVYRHLPERVLRQYGYVQTIPRHPTDVRDLPPPSIVQMFVDFRTHTLKADARG 310
W +C HLPER LR YGYVQT+P PT + L P +V F++F H RG
Sbjct: 591 WFLC-------HLPERGLR*YGYVQTVPIPPTTIEPLEPAEVVTAFLEFALHVQSQQERG 749
Query: 311 EQAGEDT-WQVADGYVLWYTRVSHPQILPP--IPGD 343
E ED + + GY+ + RVSH ++ P +P D
Sbjct: 750 ESVPEDEGHKHSKGYMK*FYRVSHLLMIAPAAVPED 857
>BE943429
Length = 384
Score = 130 bits (328), Expect = 6e-31
Identities = 59/122 (48%), Positives = 78/122 (63%), Gaps = 1/122 (0%)
Frame = +2
Query: 221 DRIQLDDVCWRSYEEHREIQDFEEVFWYSRWIMCGVRRVYRHLPERVLRQYGYVQTIPRH 280
D ++ V WR YE R++ F++V WYS WIM G +++ RHLPERVLRQYGYVQT+PR
Sbjct: 2 DSMEHCHVIWRPYEHRRDVTPFQDVCWYSGWIMAGKQKMVRHLPERVLRQYGYVQTVPRP 181
Query: 281 PTDVRDLPPPSIVQMFVDFRTHTLKADARGEQAGEDT-WQVADGYVLWYTRVSHPQILPP 339
PT + L P + F +F H L RG+ ED W+ +DGY+ W+ RVSHP I+ P
Sbjct: 182 PTTIVPLAPAEVATAFFEFVVHVLSQQDRGDPVPEDEWWKHSDGYIKWFYRVSHPLIVNP 361
Query: 340 IP 341
P
Sbjct: 362 AP 367
>BG453710 weakly similar to GP|21537057|gb| unknown {Arabidopsis thaliana},
partial (6%)
Length = 651
Score = 71.2 bits (173), Expect(2) = 5e-19
Identities = 40/95 (42%), Positives = 54/95 (56%)
Frame = -3
Query: 40 LLMTYLGVAQHEAEKICNQEYGGYISYPRLRDFYTSYLGRANVLAGTEDPEEVEELERVR 99
L+ LG++ A E G+ISYP L+ Y +L A L EE++E R R
Sbjct: 298 LMTRLLGMSDANAMAEIRTESAGHISYPTLKRVYEDHLIEARRLEDPLTREELQERARRR 119
Query: 100 TYCVRCYLLYLVGCLLFGDRSNKRIELIYLTTMAD 134
+CVR LLYLV C+LF ++N+ I+LIYL MAD
Sbjct: 118 QWCVRSLLLYLVDCVLFTYKTNRHIDLIYLDCMAD 14
Score = 40.4 bits (93), Expect(2) = 5e-19
Identities = 18/31 (58%), Positives = 23/31 (74%)
Frame = -1
Query: 1 MPMGEMTVTLDDVACLTHLSIEGRMLAHGKK 31
+PMGEMT+TLDDV L H+ I G ML H ++
Sbjct: 414 LPMGEMTITLDDVYNLLHIPIHGCMLDHDEQ 322
>BG646599
Length = 711
Score = 70.1 bits (170), Expect(2) = 9e-14
Identities = 46/150 (30%), Positives = 71/150 (46%), Gaps = 7/150 (4%)
Frame = +3
Query: 156 LADACRLGHRALGGSVTLLTAWFLAHFPGFFSVDLNTDYLENYSV----AARWKLQKGHG 211
L + + + LGG TLL W +FP + N + + A RWK +G+
Sbjct: 6 LGNGSMMSCKQLGGYATLLQCWIYEYFPTLGNRVENRVACDEPGMGVARAMRWKYPRGNL 185
Query: 212 EGITYRSLLDRIQLDDVCWRSYEEHREIQDFEEV---FWYSRWIMCGVRRVYRHLPERVL 268
+ R ++D + +DV W ++ HR++ F+++ Y RW V +LPER L
Sbjct: 186 KVDQIRRMIDDLTPNDVIWCPFDSHRQVIPFDDICLSSGYIRW----CSNVVPYLPERCL 353
Query: 269 RQYGYVQTIPRHPTDVRDLPPPSIVQMFVD 298
RQ+GY+Q IPR PPP+ VD
Sbjct: 354 RQFGYIQYIPR--------PPPNFNTFNVD 419
Score = 23.9 bits (50), Expect(2) = 9e-14
Identities = 7/13 (53%), Positives = 10/13 (76%)
Frame = +1
Query: 327 WYTRVSHPQILPP 339
WY +VSHP ++ P
Sbjct: 517 WYYKVSHPHLIRP 555
>TC82474
Length = 992
Score = 70.9 bits (172), Expect = 7e-13
Identities = 32/88 (36%), Positives = 51/88 (57%)
Frame = -2
Query: 194 YLENYSVAARWKLQKGHGEGITYRSLLDRIQLDDVCWRSYEEHREIQDFEEVFWYSRWIM 253
Y E+ A R+ L GH Y + LDR+++DDV + Y+ H + F+++ + RWI
Sbjct: 883 YTEDRPHATRFSLAMGHKSPNNYMTFLDRVEVDDVSFNQYDFHHQTCPFDDIS*FFRWIE 704
Query: 254 CGVRRVYRHLPERVLRQYGYVQTIPRHP 281
C + Y HL E V++ YG++Q+ RHP
Sbjct: 703 CDRKMNYHHLLELVMK*YGHIQSTLRHP 620
>BG454068 weakly similar to GP|22202804|dbj hypothetical protein~predicted by
GeneMark.hmm etc. {Oryza sativa (japonica
cultivar-group)}, partial (5%)
Length = 673
Score = 64.7 bits (156), Expect = 5e-11
Identities = 35/94 (37%), Positives = 51/94 (54%), Gaps = 3/94 (3%)
Frame = +3
Query: 1 MPMGEMTVTLDDVACLTHLSIEGRMLAHGKKMPKHEGAALLMTYLGVAQHEAEKICNQEY 60
+P+GEMT+ LDD++CL H+ + G +L H + + H+G L+ YLG+ E +
Sbjct: 387 LPVGEMTIALDDISCLLHIPVGGNLLFH-ESLSIHQGTEYLVNYLGLEFEEIAAETKRLK 563
Query: 61 GGYISYPRLRDFYTSYLGRANVLA---GTEDPEE 91
+I+Y L YTSYL A A G ED E
Sbjct: 564 SAHITYDTLLSIYTSYLTEAKSYANQPGEEDSME 665
>AW693426 homologue to GP|23307580|dbj similar to serine/threonine
phosphatase PP7 {Oryza sativa (japonica
cultivar-group)}, partial (2%)
Length = 209
Score = 44.3 bits (103), Expect = 7e-05
Identities = 25/69 (36%), Positives = 36/69 (51%)
Frame = +1
Query: 106 YLLYLVGCLLFGDRSNKRIELIYLTTMADGYAGMRNYSWGAMTLAYLYGELADACRLGHR 165
+LL LVGC LF D+S + + YL D Y+WG LA+LY L A +
Sbjct: 1 FLLCLVGCTLFSDKSTFVVNVAYLEFFRD-LDSCGGYAWGVAALAHLYDNLRYASFHHMK 177
Query: 166 ALGGSVTLL 174
++ G +TL+
Sbjct: 178 SISGYLTLI 204
>TC77808 similar to PIR|T03843|T03843 prohibitin - common tobacco, partial
(96%)
Length = 1202
Score = 30.0 bits (66), Expect = 1.4
Identities = 15/37 (40%), Positives = 19/37 (50%), Gaps = 1/37 (2%)
Frame = -3
Query: 226 DDVCWRSYEEHREIQDFEEVFWYSRWI-MCGVRRVYR 261
+DV ++ EH E E WY RW+ MC V YR
Sbjct: 393 EDVQYQDEREHEE*DSPSEDLWYRRWMRMCEVECGYR 283
>TC81072 similar to PIR|T05401|T05401 hypothetical protein F10M6.90 -
Arabidopsis thaliana, partial (13%)
Length = 745
Score = 28.5 bits (62), Expect = 4.2
Identities = 10/39 (25%), Positives = 25/39 (63%), Gaps = 3/39 (7%)
Frame = +2
Query: 80 ANVLAGTEDPEEVEELERV---RTYCVRCYLLYLVGCLL 115
A+++ ++ P + +R+ +T+C+RC L+Y++ C +
Sbjct: 302 ASIIGSSQKPNR*QTFQRLCYDQTWCLRCNLVYVLRCFV 418
>AW299072 weakly similar to GP|9796478|gb|A serine threonine kinase homolog
COK-4 {Phaseolus vulgaris}, partial (26%)
Length = 689
Score = 27.7 bits (60), Expect = 7.1
Identities = 21/76 (27%), Positives = 33/76 (42%)
Frame = +2
Query: 60 YGGYISYPRLRDFYTSYLGRANVLAGTEDPEEVEELERVRTYCVRCYLLYLVGCLLFGDR 119
Y GY+ Y D+ + L +V G E+E L C C+ L+ + F D+
Sbjct: 374 YKGYVKYNGENDYPIALLRITDVFRGQGFKNEIEHL------CQLCH-PNLISFIGFCDQ 532
Query: 120 SNKRIELIYLTTMADG 135
NK+I + M +G
Sbjct: 533 KNKKILVYKKDEMVNG 580
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.323 0.140 0.447
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,507,812
Number of Sequences: 36976
Number of extensions: 186579
Number of successful extensions: 858
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 852
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 855
length of query: 357
length of database: 9,014,727
effective HSP length: 97
effective length of query: 260
effective length of database: 5,428,055
effective search space: 1411294300
effective search space used: 1411294300
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 59 (27.3 bits)
Medicago: description of AC125478.7