BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC125368.2 - phase: 0
(430 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86820 similar to GP|15146218|gb|AAK83592.1 AT3g09470/F11F8_4 {... 281 0.0
TC86819 similar to GP|15146218|gb|AAK83592.1 AT3g09470/F11F8_4 {... 288 4e-78
AW256515 similar to GP|15146218|gb AT3g09470/F11F8_4 {Arabidopsi... 208 4e-54
BG450455 similar to SP|Q07423|HEX6 Hexose carrier protein HEX6. ... 32 0.62
TC77879 similar to PIR|D86442|D86442 probable amino acid permeas... 31 0.81
BG647755 29 3.1
TC80056 similar to GP|12322859|gb|AAG51421.1 unknown protein co... 29 3.1
AW686891 weakly similar to GP|10177234|db gb|AAF18257.1~gene_id:... 29 4.0
TC78603 weakly similar to GP|19698827|gb|AAL91149.1 unknown prot... 28 6.8
TC77005 similar to SP|P21653|TIP1_TOBAC Tonoplast intrinsic prot... 28 6.8
AW736472 homologue to SP|P21653|TIP1 Tonoplast intrinsic protein... 28 6.8
TC78964 weakly similar to PIR|T00450|T00450 probable monosacchar... 28 8.9
TC78468 similar to GP|21594872|gb|AAM66051.1 unknown {Arabidopsi... 28 8.9
>TC86820 similar to GP|15146218|gb|AAK83592.1 AT3g09470/F11F8_4 {Arabidopsis
thaliana}, partial (75%)
Length = 1646
Score = 281 bits (719), Expect(4) = 0.0
Identities = 133/133 (100%), Positives = 133/133 (100%)
Frame = +2
Query: 298 SPLVSRPLHR*FLLELLLRRLH*SYFC*ISAYLADFSVLHIYSFWLLYWASVMEF**HSS 357
SPLVSRPLHR*FLLELLLRRLH*SYFC*ISAYLADFSVLHIYSFWLLYWASVMEF**HSS
Sbjct: 1085 SPLVSRPLHR*FLLELLLRRLH*SYFC*ISAYLADFSVLHIYSFWLLYWASVMEF**HSS 1264
Query: 358 MLC*EYYLSMTWKGLLHS*RYGKALQLLSCSFWPRTFRSKQLP*SCWFYYACHSVVFYGW 417
MLC*EYYLSMTWKGLLHS*RYGKALQLLSCSFWPRTFRSKQLP*SCWFYYACHSVVFYGW
Sbjct: 1265 MLC*EYYLSMTWKGLLHS*RYGKALQLLSCSFWPRTFRSKQLP*SCWFYYACHSVVFYGW 1444
Query: 418 LSRWGMHHHPPPV 430
LSRWGMHHHPPPV
Sbjct: 1445 LSRWGMHHHPPPV 1483
Score = 271 bits (692), Expect(4) = 0.0
Identities = 136/138 (98%), Positives = 137/138 (98%)
Frame = +3
Query: 135 ITISTKVL*LVTSTGNSGLFMHSISLLEILLHLLYSVMGRGEVPTAPLYCLLYSFSL*PL 194
ITISTKVL*LVTSTGNSGLFMHSISLLEILLHLLYSVMGRGEVPTAPLYCLLYSFSL*PL
Sbjct: 594 ITISTKVL*LVTSTGNSGLFMHSISLLEILLHLLYSVMGRGEVPTAPLYCLLYSFSL*PL 773
Query: 195 VQY*CSSCINELVIVKKNTRIQKPMLLNQSPLSLYVNHSQVHCLMRRCC*SYPLWHIQVY 254
VQY*CSSCINELVIVKKNTRIQKPMLLNQSPLSLYVNHSQVHCLMRRCC*SYPLWHIQVY
Sbjct: 774 VQY*CSSCINELVIVKKNTRIQKPMLLNQSPLSLYVNHSQVHCLMRRCC*SYPLWHIQVY 953
Query: 255 NKHLYGLNLPSLL*LQQL 272
NKHLYGLNLPSLL*LQ +
Sbjct: 954 NKHLYGLNLPSLL*LQAI 1007
Score = 270 bits (689), Expect(4) = 0.0
Identities = 136/139 (97%), Positives = 136/139 (97%)
Frame = +2
Query: 1 MASVTIHHDEETPLVVESPIQLQPHKSHSRDVHILSIAFVLIFLAYGAAQNLQSTLNTEE 60
MASVTIHHDEETPLVVESPIQLQPHKSHSRDVHILSIAFVLIFLAYGAAQNLQSTLNTEE
Sbjct: 152 MASVTIHHDEETPLVVESPIQLQPHKSHSRDVHILSIAFVLIFLAYGAAQNLQSTLNTEE 331
Query: 61 DLGTTSLGILYLSFTFFSVFASLVVRILGSKNALIIGTSGYWLYVAANLKPNWYTLVPAS 120
DLGTTSLGILYLSFTFFSVFASLVVRILGSKNALIIGTSGYWLYVAANLKPNWYTLVPAS
Sbjct: 332 DLGTTSLGILYLSFTFFSVFASLVVRILGSKNALIIGTSGYWLYVAANLKPNWYTLVPAS 511
Query: 121 VYLGFCASIIWVGQITIST 139
VYLGFCASIIWVGQ T T
Sbjct: 512 VYLGFCASIIWVGQGTYLT 568
Score = 58.5 bits (140), Expect(4) = 0.0
Identities = 27/28 (96%), Positives = 28/28 (99%)
Frame = +1
Query: 270 QQLVFLVLVLQWQHMGLLMEYVLWLLVV 297
+QLVFLVLVLQWQHMGLLMEYVLWLLVV
Sbjct: 1000 KQLVFLVLVLQWQHMGLLMEYVLWLLVV 1083
>TC86819 similar to GP|15146218|gb|AAK83592.1 AT3g09470/F11F8_4 {Arabidopsis
thaliana}, partial (60%)
Length = 1234
Score = 288 bits (736), Expect = 4e-78
Identities = 160/243 (65%), Positives = 187/243 (76%), Gaps = 14/243 (5%)
Frame = +2
Query: 100 GY-WLYVAANLKPNWYTLVPASVYLGFCASIIWVGQ-------------ITISTKVL*LV 145
GY WL + + + W+ L+ LGF +G+ ITISTKV *LV
Sbjct: 506 GYTWLQI*SPIGIRWFRLL---FILGFALLYYGLGREHI*LPLHAVML*ITISTKVR*LV 676
Query: 146 TSTGNSGLFMHSISLLEILLHLLYSVMGRGEVPTAPLYCLLYSFSL*PLVQY*CSSCINE 205
TST +SG++ H ISLLEILLHLL+SVMGR EVP A LYCL YSF L*PLVQY* + CIN
Sbjct: 677 TSTESSGVYTHFISLLEILLHLLFSVMGRREVPMAQLYCLSYSFPL*PLVQY*RAFCINV 856
Query: 206 LVIVKKNTRIQKPMLLNQSPLSLYVNHSQVHCLMRRCC*SYPLWHIQVYNKHLYGLNLPS 265
++I +++T I P+L+NQSPLSLYV+HS+VHCLM RCC*SYPL HIQVYN LYGLNLPS
Sbjct: 857 VIIAREDTSIWMPVLVNQSPLSLYVDHSRVHCLM*RCC*SYPL*HIQVYNTLLYGLNLPS 1036
Query: 266 LL*LQQLVFLVLVLQWQHMGLLMEYVLWLLVVSPLVSRPLHR*FLLELLLRRLH*SYFC* 325
+L*LQ+LVFLVLVLQWQ+MGLLMEYVLW LVVSPLVS PLH+*FLLELL +L+*SYFC*
Sbjct: 1037ML*LQKLVFLVLVLQWQYMGLLMEYVLWSLVVSPLVSHPLHQ*FLLELLFMQLY*SYFC* 1216
Query: 326 ISA 328
ISA
Sbjct: 1217ISA 1225
Score = 249 bits (637), Expect = 1e-66
Identities = 127/142 (89%), Positives = 132/142 (92%), Gaps = 3/142 (2%)
Frame = +1
Query: 1 MASVTIHHDEETPLVV---ESPIQLQPHKSHSRDVHILSIAFVLIFLAYGAAQNLQSTLN 57
MASV IH DEETPLVV ESPIQ Q HKSH+RDVHILSIAF+LIFLAYGAAQNLQSTLN
Sbjct: 193 MASVIIHRDEETPLVVATDESPIQFQQHKSHTRDVHILSIAFLLIFLAYGAAQNLQSTLN 372
Query: 58 TEEDLGTTSLGILYLSFTFFSVFASLVVRILGSKNALIIGTSGYWLYVAANLKPNWYTLV 117
TEEDLGTTSLGILYLSFTFFSVFASLVVRILGSKNALI+GTSGYWLY+AANLKPNWYTLV
Sbjct: 373 TEEDLGTTSLGILYLSFTFFSVFASLVVRILGSKNALIVGTSGYWLYLAANLKPNWYTLV 552
Query: 118 PASVYLGFCASIIWVGQITIST 139
PASVYLGFCASI+WVGQ T T
Sbjct: 553 PASVYLGFCASILWVGQGTYLT 618
>AW256515 similar to GP|15146218|gb AT3g09470/F11F8_4 {Arabidopsis thaliana},
partial (25%)
Length = 624
Score = 208 bits (529), Expect = 4e-54
Identities = 115/149 (77%), Positives = 122/149 (81%), Gaps = 13/149 (8%)
Frame = +1
Query: 1 MASVTIHHDEETPLVV---ESPIQLQPHKSHSRDVHILSIAFVLIFLAYGAAQNLQSTLN 57
MAS IH DEETPLVV ESPIQ Q HKSH+RDVHILSIAF+LIFLAYGAAQNLQSTLN
Sbjct: 139 MASEIIHRDEETPLVVATDESPIQFQQHKSHTRDVHILSIAFLLIFLAYGAAQNLQSTLN 318
Query: 58 TEEDLGTTSLGILYLSFTFFSVFASLVVRILGSKNALIIGTSGYWLYVAANLKPNWYTLV 117
TEEDLGTTSLGILYLSFTFFSVFASLVVRILGSKNALI+GTSGYWLY+AANLKPNW V
Sbjct: 319 TEEDLGTTSLGILYLSFTFFSVFASLVVRILGSKNALIVGTSGYWLYLAANLKPNW*LQV 498
Query: 118 PA-SVYL--GFCASII-------WVGQIT 136
A VY+ GFC S + W G I+
Sbjct: 499 HAIYVYVGSGFCLSWVLRFYIMGWAGNIS 585
Score = 48.5 bits (114), Expect = 5e-06
Identities = 22/26 (84%), Positives = 23/26 (87%)
Frame = +2
Query: 114 YTLVPASVYLGFCASIIWVGQITIST 139
YTLVPASVYLGFCASI+WVGQ T T
Sbjct: 512 YTLVPASVYLGFCASILWVGQGTYLT 589
>BG450455 similar to SP|Q07423|HEX6 Hexose carrier protein HEX6. [Castor
bean] {Ricinus communis}, partial (36%)
Length = 573
Score = 31.6 bits (70), Expect = 0.62
Identities = 20/67 (29%), Positives = 32/67 (46%), Gaps = 1/67 (1%)
Frame = +2
Query: 59 EEDLGTTSLGILYLSFTFFSVFASLVVRILGSKNALIIGTSGYWLYVA-ANLKPNWYTLV 117
+ L TT LY++ S FAS + R G K ++++G + + + A N Y L+
Sbjct: 230 DSQLLTTFTSSLYIAGLLASFFASSITRAFGRKPSILVGGAAFLIGAALGGAALNIYMLI 409
Query: 118 PASVYLG 124
V LG
Sbjct: 410 LGRVLLG 430
>TC77879 similar to PIR|D86442|D86442 probable amino acid permease
[imported] - Arabidopsis thaliana, partial (81%)
Length = 2037
Score = 31.2 bits (69), Expect = 0.81
Identities = 22/90 (24%), Positives = 42/90 (46%)
Frame = +2
Query: 24 PHKSHSRDVHILSIAFVLIFLAYGAAQNLQSTLNTEEDLGTTSLGILYLSFTFFSVFASL 83
P +H R + +L + F++ + G ++ T+ L LG F +SV +L
Sbjct: 308 PRANHMRKLSVLPLVFLIFYEVSGGPFGVEDTVKAAGPL-LALLGFFVFPF-IWSVPEAL 481
Query: 84 VVRILGSKNALIIGTSGYWLYVAANLKPNW 113
+ +G+ + SGY ++V++ L P W
Sbjct: 482 ITAEMGT---MFPENSGYVVWVSSALGPFW 562
>BG647755
Length = 439
Score = 29.3 bits (64), Expect = 3.1
Identities = 14/26 (53%), Positives = 16/26 (60%)
Frame = -1
Query: 21 QLQPHKSHSRDVHILSIAFVLIFLAY 46
QLQPH + R H+ S F L FLAY
Sbjct: 292 QLQPHHADMRLFHMKSSQFELFFLAY 215
>TC80056 similar to GP|12322859|gb|AAG51421.1 unknown protein contains
TNFR/NGFR cysteine-rich region; 28705-27186 {Arabidopsis
thaliana}, partial (98%)
Length = 879
Score = 29.3 bits (64), Expect = 3.1
Identities = 19/54 (35%), Positives = 28/54 (51%)
Frame = +2
Query: 201 SCINELVIVKKNTRIQKPMLLNQSPLSLYVNHSQVHCLMRRCC*SYPLWHIQVY 254
SC N+L I+ + + L P L+ +SQVH L+R CC + L Q+Y
Sbjct: 278 SCDNQLYIL-----FCRQLFLCLYPWILFKRNSQVHKLLRCCCWNSALSFDQLY 424
>AW686891 weakly similar to GP|10177234|db
gb|AAF18257.1~gene_id:MRN17.10~similar to unknown
protein {Arabidopsis thaliana}, partial (64%)
Length = 482
Score = 28.9 bits (63), Expect = 4.0
Identities = 30/93 (32%), Positives = 39/93 (41%), Gaps = 7/93 (7%)
Frame = -3
Query: 220 LLNQSPLSLYVNHSQVHCLMRRCC*SYPLWHIQVYNKHLYGLNLPSL-L*LQQLVFLVLV 278
L N L L N S + + *S L H+Q Y L L +L + L Q L L
Sbjct: 282 LWNLDSLILL*NQSLHRGDLHQLN*SCGLKHLQHYIASFLALQLANL*VQLSQQ*LLKLK 103
Query: 279 LQWQHMGLLMEYVLWLL------VVSPLVSRPL 305
L WQ + +++WLL V P RPL
Sbjct: 102 LLWQCNVAALNFLVWLLEDLIV*EVEPFFERPL 4
>TC78603 weakly similar to GP|19698827|gb|AAL91149.1 unknown protein
{Arabidopsis thaliana}, partial (28%)
Length = 1559
Score = 28.1 bits (61), Expect = 6.8
Identities = 18/62 (29%), Positives = 26/62 (41%), Gaps = 14/62 (22%)
Frame = +3
Query: 7 HHDEETPLVVESPIQLQP----HKSHSRDVHILSIA----------FVLIFLAYGAAQNL 52
+HD P +SP+ P H H D+H L IA +L F + + +N
Sbjct: 117 YHDSPNPSPQQSPVTYTPYNILHHFHIHDIHTLRIAMMAMACFVTTIMLFFFFFISIRNY 296
Query: 53 QS 54
QS
Sbjct: 297 QS 302
>TC77005 similar to SP|P21653|TIP1_TOBAC Tonoplast intrinsic protein
root-specific RB7-5A (RT-TIP). [Common tobacco]
{Nicotiana tabacum}, partial (97%)
Length = 1055
Score = 28.1 bits (61), Expect = 6.8
Identities = 8/13 (61%), Positives = 9/13 (68%)
Frame = +3
Query: 415 YGWLSRWGMHHHP 427
Y W+S W HHHP
Sbjct: 369 YFWISHWRQHHHP 407
>AW736472 homologue to SP|P21653|TIP1 Tonoplast intrinsic protein
root-specific RB7-5A (RT-TIP). [Common tobacco]
{Nicotiana tabacum}, partial (43%)
Length = 691
Score = 28.1 bits (61), Expect = 6.8
Identities = 8/13 (61%), Positives = 9/13 (68%)
Frame = -1
Query: 415 YGWLSRWGMHHHP 427
Y W+S W HHHP
Sbjct: 415 YFWISHWRQHHHP 377
>TC78964 weakly similar to PIR|T00450|T00450 probable monosaccharide
transport protein T14N5.7 - Arabidopsis thaliana,
partial (98%)
Length = 1771
Score = 27.7 bits (60), Expect = 8.9
Identities = 18/56 (32%), Positives = 26/56 (46%), Gaps = 1/56 (1%)
Frame = +1
Query: 70 LYLSFTFFSVFASLVVRILGSKNALIIGTSGYWLYVAAN-LKPNWYTLVPASVYLG 124
LY S + FAS + R G K +I+G + + N N TL+ V+LG
Sbjct: 304 LYFSALVMTFFASYLTRNKGRKATIIVGALSFLIGAILNAAAQNIPTLIIGRVFLG 471
>TC78468 similar to GP|21594872|gb|AAM66051.1 unknown {Arabidopsis
thaliana}, partial (78%)
Length = 1249
Score = 27.7 bits (60), Expect = 8.9
Identities = 21/85 (24%), Positives = 38/85 (44%), Gaps = 6/85 (7%)
Frame = +3
Query: 221 LNQSPLSLYVNHSQVHCLMRRCC*SYPLWHIQVYNKHLYGLNLPSLL*LQQLVFLVLVLQ 280
+NQ PLSL+ + + C + + P W + +++ L+ + L F +L+L
Sbjct: 117 INQ-PLSLFPPNINMDCCIISKLKTQPFWFLILFSLGLFTI----------LRFTLLLLN 263
Query: 281 WQHMGLLME------YVLWLLVVSP 299
W ++ L + Y W LV P
Sbjct: 264 WFYVNFLRQPKNLKNYGSWALVTGP 338
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.344 0.150 0.524
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,035,619
Number of Sequences: 36976
Number of extensions: 316468
Number of successful extensions: 3676
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 2680
Number of HSP's successfully gapped in prelim test: 120
Number of HSP's that attempted gapping in prelim test: 920
Number of HSP's gapped (non-prelim): 2904
length of query: 430
length of database: 9,014,727
effective HSP length: 99
effective length of query: 331
effective length of database: 5,354,103
effective search space: 1772208093
effective search space used: 1772208093
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.6 bits)
S2: 60 (27.7 bits)
Medicago: description of AC125368.2