BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124970.11 + phase: 0
(211 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG587150 similar to GP|13592175|gb ppg3 {Leishmania major}, part... 39 0.001
BE997611 37 0.006
TC79394 weakly similar to PIR|T49019|T49019 probable RNA binding... 37 0.007
TC81816 similar to GP|8096269|dbj|BAA95789.1 KED {Nicotiana taba... 35 0.021
TC83625 similar to GP|8096269|dbj|BAA95789.1 KED {Nicotiana taba... 35 0.021
TC87237 similar to GP|160409|gb|AAA29651.1|| mature-parasite-inf... 33 0.061
TC82232 similar to GP|10177316|dbj|BAB10642. kinesin-like protei... 33 0.080
TC87387 homologue to PIR|T47775|T47775 hypothetical protein F24I... 32 0.14
BG587305 similar to GP|4249414|gb|A unknown protein {Arabidopsis... 30 0.68
TC87518 weakly similar to PIR|E96714|E96714 probable DNA-binding... 30 0.68
BF004047 weakly similar to GP|15027957|gb| putative arginine/ser... 30 0.88
TC78972 weakly similar to GP|21592657|gb|AAM64606.1 unknown {Ara... 30 0.88
BE998238 similar to PIR|T39202|T39 heat shock protein 90 homolog... 29 1.2
TC78324 similar to GP|8567798|gb|AAF76370.1| unknown protein {Ar... 28 2.0
TC80288 weakly similar to PIR|T06029|T06029 hypothetical protein... 28 2.0
TC78402 weakly similar to PIR|B84853|B84853 hypothetical protein... 28 2.6
TC80941 weakly similar to PIR|T39903|T39903 serine-rich protein ... 28 2.6
BQ141756 similar to GP|19386784|dbj hypothetical protein {Oryza ... 28 2.6
BQ141783 homologue to GP|13129535|gb| transcriptional repressor ... 28 2.6
TC86916 similar to GP|3776080|emb|CAA77093.1 MtN9 {Medicago trun... 28 2.6
>BG587150 similar to GP|13592175|gb ppg3 {Leishmania major}, partial (1%)
Length = 754
Score = 38.9 bits (89), Expect = 0.001
Identities = 28/125 (22%), Positives = 55/125 (43%), Gaps = 8/125 (6%)
Frame = +2
Query: 81 HLAKNKKKEEGQFKKFMQLFSQLQVNIPFGEALDQMPVYAKFMKEMLTSR--------RK 132
H+ +N +K + + K ++ + + F + + +P+ F + T R R+
Sbjct: 83 HMGRNLRKRKKKVAKHLKRGANEKEMESFQKRVFMIPLEKPFEEAYFTHRLWMFFRETRE 262
Query: 133 PKDDENIALSENCSAILQRKLPPKLKDPGAFTIPCSIGPVDIGRALCDLGASINLMPLSM 192
++D E + +R K D G F I ++ ++ ALC+ GAS+ ++P M
Sbjct: 263 TEEDIRRMFCEAREKMKKRITLKKKSDSGKFAISWTMKGIEFPHALCNTGASVIILPRDM 442
Query: 193 MKKLG 197
LG
Sbjct: 443 ADHLG 457
>BE997611
Length = 547
Score = 37.0 bits (84), Expect = 0.006
Identities = 21/88 (23%), Positives = 40/88 (44%)
Frame = -2
Query: 78 PYPHLAKNKKKEEGQFKKFMQLFSQLQVNIPFGEALDQMPVYAKFMKEMLTSRRKPKDDE 137
P A +K+ +F + +VN+P E ++Q+P KF++E+L + ++E
Sbjct: 468 PQTEDA*EEKEVPPKFNALHHILKGYKVNMPITETVEQIPCCIKFLQELLKTNANLSEEE 289
Query: 138 NIALSENCSAILQRKLPPKLKDPGAFTI 165
I+LS + + G FT+
Sbjct: 288 FISLSSEFHHTYEVPAVVRFDGEGCFTL 205
>TC79394 weakly similar to PIR|T49019|T49019 probable RNA binding protein -
Arabidopsis thaliana, partial (74%)
Length = 1909
Score = 36.6 bits (83), Expect = 0.007
Identities = 19/62 (30%), Positives = 38/62 (60%)
Frame = +2
Query: 1 MEVDKDVEVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEKLIDEDSILRKSKSQ 60
+++D D + +E E+E E+ EVE E + E E ++++ E+S+ L +ED +K ++
Sbjct: 236 VDLDGDNDQEESSEEEVEYEEVEVEEEVEEEEEEEEEEEVEEESKPLDEEDEADKKKHAE 415
Query: 61 IL 62
+L
Sbjct: 416 LL 421
>TC81816 similar to GP|8096269|dbj|BAA95789.1 KED {Nicotiana tabacum},
partial (13%)
Length = 663
Score = 35.0 bits (79), Expect = 0.021
Identities = 25/95 (26%), Positives = 47/95 (49%)
Frame = +3
Query: 1 MEVDKDVEVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEKLIDEDSILRKSKSQ 60
+E++KD+E+K V + + + + +KE +E K KK EK +K E+++ +
Sbjct: 132 VEIEKDLEIKSVEKDDEKKEKKDKEKKDKTDVDEGKDKKDKEKKKKEKKEENVKGEE--- 302
Query: 61 ILKDGDKPQVIPSYVKLPYPHLAKNKKKEEGQFKK 95
+DGD+ + K +KKE+G+ K
Sbjct: 303 --EDGDEKK---------DKEKKKKEKKEKGKEDK 374
Score = 35.0 bits (79), Expect = 0.021
Identities = 22/64 (34%), Positives = 35/64 (54%)
Frame = +3
Query: 4 DKDVEVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEKLIDEDSILRKSKSQILK 63
DK + K+ EK+ E+ + E+E + E+KKK+ EK ++ D+D +KSK K
Sbjct: 240 DKKDKEKKKKEKKEENVKGEEEDGDEKKDKEKKKKEKKEKGKEDKDKDGEEKKSKKDKEK 419
Query: 64 DGDK 67
DK
Sbjct: 420 KKDK 431
Score = 33.5 bits (75), Expect = 0.061
Identities = 26/92 (28%), Positives = 43/92 (46%)
Frame = +3
Query: 4 DKDVEVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEKLIDEDSILRKSKSQILK 63
DKD + +E K+ + + +K D E+ KKKK +K EK +ED ++ ++
Sbjct: 369 DKDKDGEEKKSKKDKEKKKDKNEDDDEGEDGSKKKKNKDKKEKKKEEDE--KEEGKVSVR 542
Query: 64 DGDKPQVIPSYVKLPYPHLAKNKKKEEGQFKK 95
D D + + K KKKE+ + KK
Sbjct: 543 DIDIEETAKEGKE-------KKKKKEDKEEKK 617
Score = 27.3 bits (59), Expect = 4.4
Identities = 19/63 (30%), Positives = 32/63 (50%)
Frame = +3
Query: 8 EVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEKLIDEDSILRKSKSQILKDGDK 67
E K +EK+ E VEK ++E+K+KK EK +K ++ +K K + K+ +
Sbjct: 123 EDKVEIEKDLEIKSVEK-------DDEKKEKKDKEKKDKTDVDEGKDKKDKEKKKKEKKE 281
Query: 68 PQV 70
V
Sbjct: 282 ENV 290
>TC83625 similar to GP|8096269|dbj|BAA95789.1 KED {Nicotiana tabacum},
partial (9%)
Length = 908
Score = 35.0 bits (79), Expect = 0.021
Identities = 22/64 (34%), Positives = 35/64 (54%)
Frame = +2
Query: 4 DKDVEVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEKLIDEDSILRKSKSQILK 63
DK + K+ EK+ E+ + E+E + E+KKK+ EK ++ D+D +KSK K
Sbjct: 632 DKKDKEKKKKEKKEENVKGEEEDGDEKKDKEKKKKEKKEKGKEDKDKDGEEKKSKKDKEK 811
Query: 64 DGDK 67
DK
Sbjct: 812 KKDK 823
Score = 35.0 bits (79), Expect = 0.021
Identities = 25/95 (26%), Positives = 47/95 (49%)
Frame = +2
Query: 1 MEVDKDVEVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEKLIDEDSILRKSKSQ 60
+E++KD+E+K V + + + + +KE +E K KK EK +K E+++ +
Sbjct: 524 VEIEKDLEIKSVEKDDEKKEKKDKEKKDKTDVDEGKDKKDKEKKKKEKKEENVKGEE--- 694
Query: 61 ILKDGDKPQVIPSYVKLPYPHLAKNKKKEEGQFKK 95
+DGD+ + K +KKE+G+ K
Sbjct: 695 --EDGDEKK---------DKEKKKKEKKEKGKEDK 766
Score = 27.3 bits (59), Expect = 4.4
Identities = 19/63 (30%), Positives = 32/63 (50%)
Frame = +2
Query: 8 EVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEKLIDEDSILRKSKSQILKDGDK 67
E K +EK+ E VEK ++E+K+KK EK +K ++ +K K + K+ +
Sbjct: 515 EDKVEIEKDLEIKSVEK-------DDEKKEKKDKEKKDKTDVDEGKDKKDKEKKKKEKKE 673
Query: 68 PQV 70
V
Sbjct: 674 ENV 682
>TC87237 similar to GP|160409|gb|AAA29651.1|| mature-parasite-infected
erythrocyte surface antigen {Plasmodium falciparum},
partial (2%)
Length = 2007
Score = 33.5 bits (75), Expect = 0.061
Identities = 19/59 (32%), Positives = 31/59 (52%)
Frame = +1
Query: 2 EVDKDVEVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEKLIDEDSILRKSKSQ 60
++ ++ E + EK + E K+ ++ ENE KK +GGEK I E+ KSK +
Sbjct: 193 KIQQENEENKDEEKSQQENEENKDEEKSQQENELKKNEGGEKETGEITEE----KSKQE 357
>TC82232 similar to GP|10177316|dbj|BAB10642. kinesin-like protein
{Arabidopsis thaliana}, partial (21%)
Length = 1181
Score = 33.1 bits (74), Expect = 0.080
Identities = 19/59 (32%), Positives = 34/59 (57%)
Frame = +3
Query: 6 DVEVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEKLIDEDSILRKSKSQILKD 64
DVE E+ EKE EH +++ D+ + E + KK + E KL++ S+L++ + L +
Sbjct: 147 DVEPVEIHEKELEHSSAQQKLDRELKELD-KKLEQKEAEMKLVNNASVLKQHYEKKLNE 320
>TC87387 homologue to PIR|T47775|T47775 hypothetical protein F24I3.230 -
Arabidopsis thaliana, partial (14%)
Length = 1074
Score = 32.3 bits (72), Expect = 0.14
Identities = 29/105 (27%), Positives = 52/105 (48%), Gaps = 11/105 (10%)
Frame = +3
Query: 2 EVDKDVEVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEKLIDEDSILRKSKSQI 61
EVD + +VK K+ + VE E D+ E ++KKKK E DE+ + ++ K +
Sbjct: 315 EVDGEEKVKT---KKVDDAAVEVEDDKK--EKKKKKKKDKENGAAASDEEKVEKEKKKKH 479
Query: 62 LKDGD--KPQVIPSYVK---------LPYPHLAKNKKKEEGQFKK 95
+ G+ P+V S K + P + K++KK++ + K+
Sbjct: 480 KEKGEDGSPEVEKSDKKKKKHKETSEVGSPEVDKSEKKKKKKDKE 614
>BG587305 similar to GP|4249414|gb|A unknown protein {Arabidopsis thaliana},
partial (13%)
Length = 696
Score = 30.0 bits (66), Expect = 0.68
Identities = 19/53 (35%), Positives = 33/53 (61%), Gaps = 2/53 (3%)
Frame = +2
Query: 4 DKDVEVKEVVEKETEHGEVEKESDQGVVENERKKKKGG--EKSEKLIDEDSIL 54
D +E+ +VVE +TE E K+ D+ VVE+ R GG + +E +++DS++
Sbjct: 437 DVTLELHKVVENKTESVEGVKKIDEEVVESVR---NGGVDDPAEVTVEDDSLV 586
>TC87518 weakly similar to PIR|E96714|E96714 probable DNA-binding protein
T6L1.19 [imported] - Arabidopsis thaliana, partial (41%)
Length = 1337
Score = 30.0 bits (66), Expect = 0.68
Identities = 31/123 (25%), Positives = 55/123 (44%), Gaps = 16/123 (13%)
Frame = +2
Query: 32 ENERKKKK-----GGEKSEKLIDEDS---ILRKSKSQILKDGDKPQVI-PSYVKLPYPHL 82
EN KK+K G + E+L+D+D I + S + + + +V P K PH
Sbjct: 23 ENLFKKRKMARSAKGHQDEELVDDDDDQDIFTPNTSSSINNNNNVKVDEPIRGKRANPHR 202
Query: 83 AKNKKKEEGQFKKFMQLFSQLQVNIPFGEA-------LDQMPVYAKFMKEMLTSRRKPKD 135
+K+ + E+ + K + F L+ IP ++ L ++ Y F++E L P +
Sbjct: 203 SKHSETEQRRRSKINERFQALRDLIPENDSKRDKASFLLEVIEYIHFLQEKLQIYEHPYE 382
Query: 136 DEN 138
N
Sbjct: 383 GWN 391
>BF004047 weakly similar to GP|15027957|gb| putative arginine/serine-rich
protein {Arabidopsis thaliana}, partial (11%)
Length = 835
Score = 29.6 bits (65), Expect = 0.88
Identities = 20/66 (30%), Positives = 32/66 (48%)
Frame = +1
Query: 4 DKDVEVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEKLIDEDSILRKSKSQILK 63
DKD E ++ VE ETE + E ++ + N R +K +K EK RK ++ +
Sbjct: 232 DKDEEQEKEVEVETERSDEESNNESKKLWNLRPRKP-TKKEEKKSGGGGSSRKPTTRSQR 408
Query: 64 DGDKPQ 69
+ PQ
Sbjct: 409 KAEMPQ 426
>TC78972 weakly similar to GP|21592657|gb|AAM64606.1 unknown {Arabidopsis
thaliana}, partial (27%)
Length = 1396
Score = 29.6 bits (65), Expect = 0.88
Identities = 20/62 (32%), Positives = 36/62 (57%), Gaps = 5/62 (8%)
Frame = -3
Query: 2 EVDKDVEVKEVVEKETEHGEVEKESDQG-----VVENERKKKKGGEKSEKLIDEDSILRK 56
E K +EV++ EKE E E E+E ++ +++ ERK++KG E S L +++ ++
Sbjct: 386 EKQKTMEVEDR-EKEEEEEEKEREIERE*EKKRIIKEERKERKG*EGSRFLCFCETLTKR 210
Query: 57 SK 58
K
Sbjct: 209 KK 204
>BE998238 similar to PIR|T39202|T39 heat shock protein 90 homolog - fission
yeast (Schizosaccharomyces pombe), partial (30%)
Length = 654
Score = 29.3 bits (64), Expect = 1.2
Identities = 42/164 (25%), Positives = 66/164 (39%), Gaps = 21/164 (12%)
Frame = +3
Query: 2 EVDKDVEVKEVVEKETEHGEVEK-ESDQGVVENERKKKKGGEKSEKLIDEDSILRKSKSQ 60
EVD D EVKE E++ E ++E+ E ++ E ++KK K E + +++ L Q
Sbjct: 117 EVDDDEEVKE--EEDGEKPKIEEVEDEESKKEKKKKKVKQTEVENQELNKMKPLWTRNPQ 290
Query: 61 ILKDGDKPQVIPSYVKLPYPHLAKNKKKEEGQ--FKKFM--------QLFS--------Q 102
+ + S HLA EGQ FK + LF +
Sbjct: 291 DITSEEYASFYKSLSNDWEEHLAVKHFSVEGQLEFKAILFIPKRAPFDLFETKKKRNNIK 470
Query: 103 LQVNIPF--GEALDQMPVYAKFMKEMLTSRRKPKDDENIALSEN 144
L V F + D +P Y F+K ++ S P + L +N
Sbjct: 471 LYVRRVFIMDDCEDLIPEYLNFVKGIVDSEDLPLNISRETLQQN 602
>TC78324 similar to GP|8567798|gb|AAF76370.1| unknown protein {Arabidopsis
thaliana}, complete
Length = 1108
Score = 28.5 bits (62), Expect = 2.0
Identities = 20/60 (33%), Positives = 28/60 (46%)
Frame = +1
Query: 33 NERKKKKGGEKSEKLIDEDSILRKSKSQILKDGDKPQVIPSYVKLPYPHLAKNKKKEEGQ 92
NER K+G EK+ D L + K QI K +P + +K + K K+ EGQ
Sbjct: 229 NERITKRGDTVDEKIKKLDVELNRYKDQIKK--TRPGPMQDGIKARAMRVLKQKRMYEGQ 402
>TC80288 weakly similar to PIR|T06029|T06029 hypothetical protein T28I19.100
- Arabidopsis thaliana, partial (14%)
Length = 1460
Score = 28.5 bits (62), Expect = 2.0
Identities = 36/156 (23%), Positives = 63/156 (40%), Gaps = 9/156 (5%)
Frame = +3
Query: 3 VDKDVEVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEKLIDEDSILRKSKSQIL 62
VD++ + +E + ETE+ + E E G+VEN + + E+ K D S +
Sbjct: 696 VDEEKDKEEEGDDETENEDKEDEEKGGLVEN-HENHEAREEHYKADDASSAVAH------ 854
Query: 63 KDGDKPQVIPSYVKLPYPHLA-KNKKKEEGQFKKFMQLFSQLQVN---IPFGEALDQMPV 118
D + L + L+ +N +K E + + + V+ + GE D +
Sbjct: 855 ---DTHETSTETGNLEHSDLSLQNTRKPENETNHSDESYGSQNVSDLKVTEGELTDGVSS 1025
Query: 119 YAKFMKE-----MLTSRRKPKDDENIALSENCSAIL 149
A KE K K D + LS N +A++
Sbjct: 1026NATAGKETGNDSFSNETAKTKPDSQLDLSSNLTAVI 1133
>TC78402 weakly similar to PIR|B84853|B84853 hypothetical protein At2g42370
[imported] - Arabidopsis thaliana, partial (14%)
Length = 1982
Score = 28.1 bits (61), Expect = 2.6
Identities = 18/49 (36%), Positives = 31/49 (62%)
Frame = +2
Query: 10 KEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEKLIDEDSILRKSK 58
KE+ E ET EVE E ++GVV++E ++ GE ++ +ED ++ K +
Sbjct: 398 KELFE-ETLVVEVEGEGEEGVVKDEEEE---GEAKDEEEEEDGVVVKDE 532
>TC80941 weakly similar to PIR|T39903|T39903 serine-rich protein - fission
yeast (Schizosaccharomyces pombe), partial (15%)
Length = 557
Score = 28.1 bits (61), Expect = 2.6
Identities = 16/47 (34%), Positives = 25/47 (53%)
Frame = +2
Query: 4 DKDVEVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEKLIDE 50
DK+ + K EKE E EKE + E ++K+ G+ +EK +E
Sbjct: 104 DKEEKEKIEAEKEAEGSIEEKEEGNDEEKTEVEEKEEGDDNEKSEEE 244
>BQ141756 similar to GP|19386784|dbj hypothetical protein {Oryza sativa
(japonica cultivar-group)}, partial (7%)
Length = 1219
Score = 28.1 bits (61), Expect = 2.6
Identities = 16/38 (42%), Positives = 22/38 (57%), Gaps = 2/38 (5%)
Frame = +2
Query: 4 DKDVEVKEVVEK--ETEHGEVEKESDQGVVENERKKKK 39
DK +E + V K E + G +E D+ + E ERKKKK
Sbjct: 557 DKQIEARAVKNKIKEKKKG*YWEEKDKIIEEGERKKKK 670
>BQ141783 homologue to GP|13129535|gb| transcriptional repressor scratch
{Homo sapiens}, partial (4%)
Length = 1207
Score = 28.1 bits (61), Expect = 2.6
Identities = 19/66 (28%), Positives = 26/66 (38%)
Frame = +1
Query: 21 EVEKESDQGVVENERKKKKGGEKSEKLIDEDSILRKSKSQILKDGDKPQVIPSYVKLPYP 80
E + S +G E ER +GG + E+ D ++ I K Q S YP
Sbjct: 301 EGRRGSKRGPTEEERGADRGGGQEERARDPGGSATRAPKTITPKHAKNQGGRSAPSTSYP 480
Query: 81 HLAKNK 86
H NK
Sbjct: 481 HAHPNK 498
>TC86916 similar to GP|3776080|emb|CAA77093.1 MtN9 {Medicago truncatula},
partial (39%)
Length = 954
Score = 28.1 bits (61), Expect = 2.6
Identities = 24/87 (27%), Positives = 40/87 (45%), Gaps = 8/87 (9%)
Frame = +2
Query: 42 EKSEKLIDEDS--------ILRKSKSQILKDGDKPQVIPSYVKLPYPHLAKNKKKEEGQF 93
EK +K+I+ + ++ K ++++ K V +K P P K K Q
Sbjct: 110 EKFDKVINSEGSDIAWDKFVMDPLKDKVMESEAKKMV--ENIK-PSPPSQLLKYKGLDQI 280
Query: 94 KKFMQLFSQLQVNIPFGEALDQMPVYA 120
K+++Q F L+ + PF LDQ V A
Sbjct: 281 KQYLQNFGYLEQSGPFNNTLDQETVLA 361
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.313 0.134 0.369
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,077,536
Number of Sequences: 36976
Number of extensions: 60814
Number of successful extensions: 407
Number of sequences better than 10.0: 59
Number of HSP's better than 10.0 without gapping: 392
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 400
length of query: 211
length of database: 9,014,727
effective HSP length: 92
effective length of query: 119
effective length of database: 5,612,935
effective search space: 667939265
effective search space used: 667939265
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 56 (26.2 bits)
Medicago: description of AC124970.11