BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124967.4 - phase: 0 /pseudo
(335 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC89627 weakly similar to GP|17529290|gb|AAL38872.1 putative pec... 153 1e-37
TC86464 similar to GP|2098711|gb|AAB57670.1| pectinesterase {Cit... 107 4e-24
BE317744 similar to GP|17529290|gb putative pectin methylesteras... 108 4e-24
TC91054 similar to GP|20521423|dbj|BAB91933. putative pectin met... 107 8e-24
NP212791 NP212791|AJ249611.1|CAB65291.1 pectin methyl-esterase PEF1 107 8e-24
TC86403 similar to PIR|B86158|B86158 hypothetical protein AAF028... 104 4e-23
TC87503 similar to SP|Q43111|PME3_PHAVU Pectinesterase 3 precurs... 103 7e-23
TC76734 similar to PIR|T06468|T06468 pectinesterase (EC 3.1.1.11... 100 1e-21
TC76732 homologue to PIR|T06468|T06468 pectinesterase (EC 3.1.1.... 100 1e-21
TC77013 similar to GP|7025485|gb|AAF35897.1| pectin methylestera... 97 7e-21
TC91752 similar to GP|6630559|gb|AAF19578.1| putative pectineste... 92 2e-19
TC77735 similar to GP|14334646|gb|AAK59501.1 putative pectineste... 88 2e-19
TC85623 similar to GP|13605696|gb|AAK32841.1 At2g45220/F4L23.27 ... 92 3e-19
TC76733 similar to PIR|T06468|T06468 pectinesterase (EC 3.1.1.11... 92 3e-19
AL368659 similar to PIR|B86158|B86 hypothetical protein AAF02886... 88 5e-18
BE319906 similar to SP|Q43062|PME_ Pectinesterase PPE8B precurso... 87 1e-17
BQ138825 similar to PIR|T49922|T49 pectin methylesterase-like pr... 87 1e-17
BI272853 weakly similar to PIR|T04359|T04 pectin methylesterase-... 73 1e-13
TC84419 similar to SP|Q42920|PME_MEDSA Pectinesterase precursor ... 73 2e-13
TC92167 similar to PIR|T05203|T05203 pectinesterase homolog F4I1... 49 2e-06
>TC89627 weakly similar to GP|17529290|gb|AAL38872.1 putative pectin
methylesterase {Arabidopsis thaliana}, partial (33%)
Length = 1289
Score = 153 bits (386), Expect = 1e-37
Identities = 86/195 (44%), Positives = 122/195 (62%), Gaps = 4/195 (2%)
Frame = +3
Query: 40 LEEHLTAAETTVRVVRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDR 99
L+ L AE+ ++V +DG+ F ++T+A+ SI N RRV++ I G YREKI V +
Sbjct: 195 LDPKLKKAESNKVRLKVSQDGSAQFKSITEALNSIQPYNIRRVIISIAPGYYREKIVVPK 374
Query: 100 SKRFVTFYGERNGKD----NDMMPIITYDATALRYGTLDSATVAVDADYFVAVNVAFVNS 155
+ F+TF G+ ND + D LR T +SATVAV+A YF+A+N+ F N+
Sbjct: 375 TLPFITFLGDVRDPPTITGNDTQSVTGSDGAQLR--TFNSATVAVNASYFMAININFENT 548
Query: 156 SPMPDENSVGGQALAMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFG 215
+ P + V QA+A+RI+G+K AFYNC F G QDTL D G H+F +C I+G+ DFI G
Sbjct: 549 ASFPIGSKVE-QAVAVRITGNKTAFYNCTFSGVQDTLYDHKGLHYFNNCTIKGSVDFICG 725
Query: 216 NGKSIYLRTTIESVA 230
+GKS+Y TI S+A
Sbjct: 726 HGKSLYEGCTIRSIA 770
>TC86464 similar to GP|2098711|gb|AAB57670.1| pectinesterase {Citrus
sinensis}, partial (85%)
Length = 1993
Score = 107 bits (268), Expect(2) = 4e-24
Identities = 67/189 (35%), Positives = 99/189 (51%), Gaps = 1/189 (0%)
Frame = +1
Query: 41 EEHLTAAETTVRVVRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRS 100
+ L T V V DG+G+F TV++AV + P + +R V+ I G Y+E + V +
Sbjct: 844 DRRLLQGSTVKADVVVAADGSGNFKTVSEAVAAAPLKSSKRYVIKIKAGVYKENVEVPKK 1023
Query: 101 KRFVTFYGERNGKDNDMMPIITYDATALRYGT-LDSATVAVDADYFVAVNVAFVNSSPMP 159
K + F G+ G+ N IIT + T SATVA+ F+A ++ F N++
Sbjct: 1024 KTNIMFLGD--GRTNT---IITGSRNVVDGSTTFHSATVAIVGGNFLARDITFQNTA--- 1179
Query: 160 DENSVGGQALAMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKS 219
QA+A+R+ D +AFYNC I +QDTL + FF +CFI GT DFIFGN
Sbjct: 1180 --GPAKHQAVALRVGADLSAFYNCDIIAYQDTLYVHNNRQFFVNCFISGTVDFIFGNSAV 1353
Query: 220 IYLRTTIES 228
++ I +
Sbjct: 1354 VFQNCDIHA 1380
Score = 21.2 bits (43), Expect(2) = 4e-24
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = +3
Query: 237 HSTR*GKNVGRHW 249
HSTR*G + +HW
Sbjct: 1413 HSTR*G*S*SKHW 1451
>BE317744 similar to GP|17529290|gb putative pectin methylesterase
{Arabidopsis thaliana}, partial (29%)
Length = 345
Score = 108 bits (269), Expect = 4e-24
Identities = 47/90 (52%), Positives = 66/90 (73%)
Frame = +2
Query: 131 GTLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQALAMRISGDKAAFYNCKFIGFQD 190
GT SAT AV++ YF+A N+ F N+ P+P ++G QA+A RIS D A+F C+F+G QD
Sbjct: 74 GTYGSATFAVNSPYFIAKNITFKNTFPLPAPGAIGKQAVAFRISADTASFLGCRFLGAQD 253
Query: 191 TLCDDYGKHFFKDCFIQGTYDFIFGNGKSI 220
TL D G+H++KDC+I+G+ DFIFGN S+
Sbjct: 254 TLYDHIGRHYYKDCYIEGSVDFIFGNALSL 343
>TC91054 similar to GP|20521423|dbj|BAB91933. putative pectin methylesterase
{Oryza sativa (japonica cultivar-group)}, partial (42%)
Length = 809
Score = 107 bits (266), Expect = 8e-24
Identities = 62/169 (36%), Positives = 94/169 (54%), Gaps = 10/169 (5%)
Frame = +1
Query: 62 GDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGERNGKDNDMMPII 121
G F+++ A+ S+P N RVV+ + G Y EK+++ K F+T G K I+
Sbjct: 310 GGFSSIQAAIDSLPFINLVRVVIKVHAGVYTEKVSIPALKSFITIQGAGADKT-----IV 474
Query: 122 TYDATALR---------YGTLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQALAMR 172
+ TAL GT SAT AV++ YF+A N+ F N++P+P +VG Q +A+R
Sbjct: 475 QWGDTALTPNPGAKGQTLGTYGSATFAVNSPYFIAKNITFKNTAPIPKPEAVGKQGVALR 654
Query: 173 ISGDKAAFYNCKFI-GFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSI 220
IS D A + + G T D G+H++KD +I+G+ DFIFGN S+
Sbjct: 655 ISADTAMVLGLQILRGTGHTWYDHIGRHYYKDWYIEGSVDFIFGNALSL 801
>NP212791 NP212791|AJ249611.1|CAB65291.1 pectin methyl-esterase PEF1
Length = 1698
Score = 107 bits (266), Expect = 8e-24
Identities = 64/199 (32%), Positives = 104/199 (52%), Gaps = 6/199 (3%)
Frame = +1
Query: 43 HLTAAETTVRVVRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKR 102
HL A V +DG+G F T+TDA+K++P N V+++ G Y+E + V +
Sbjct: 715 HLLAGGNVKANAVVAQDGSGQFKTLTDALKTVPPTNAAPFVIYVKAGVYKETVNVAKEMN 894
Query: 103 FVTFYGERNGKDNDMMPIITYDATALRYG----TLDSATVAVDADYFVAVNVAFVNSSPM 158
+VT G+ P T +L Y T +AT V+ F+A ++ F N++
Sbjct: 895 YVTVIGDG--------PTKTKFTGSLNYADGINTYKTATFGVNGANFMAKDIGFENTA-- 1044
Query: 159 PDENSVGGQALAMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGK 218
+ QA+A+R++ D+A F+NC+ GFQDTL + + F++DC I GT DF+FG+
Sbjct: 1045---GTSKFQAVALRVTADQAIFHNCQMDGFQDTLFVESQRQFYRDCAISGTIDFVFGDAF 1215
Query: 219 SIY--LRTTIESVAKGFEC 235
++ + AKG +C
Sbjct: 1216GVFQNCKLICRVPAKGQKC 1272
>TC86403 similar to PIR|B86158|B86158 hypothetical protein AAF02886.1
[imported] - Arabidopsis thaliana, partial (63%)
Length = 1929
Score = 104 bits (260), Expect = 4e-23
Identities = 64/188 (34%), Positives = 104/188 (55%), Gaps = 5/188 (2%)
Frame = +3
Query: 39 LLEEHLTAAETTVR-VVRVRKDGTGDFTTVTDAVKSIPS---GNKRRVVVWIGMGEYREK 94
LL+ + + VR +V V +DG+G+FTT+ DA+ + P+ + ++++ G Y E
Sbjct: 720 LLQSNQVGEDVVVRDIVTVSQDGSGNFTTINDAIAAAPNKSVSSDGYFLIYVTAGVYEEY 899
Query: 95 ITVDRSKRFVTFYGERNGKDNDMMPIITYDATALR-YGTLDSATVAVDADYFVAVNVAFV 153
IT+D+ K ++ G+ K IIT + + + + T S T AV FV VN+
Sbjct: 900 ITIDKKKTYLMMIGDGINKT-----IITGNHSVVDGWTTFGSPTFAVVGQGFVGVNMTIR 1064
Query: 154 NSSPMPDENSVGGQALAMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFI 213
N++ +V QA+A+R D + FY+C F G+QDTL + F+++C I GT DFI
Sbjct: 1065NTA-----GAVKHQAVALRNGADLSTFYSCSFEGYQDTLYTHSLRQFYRECDIYGTVDFI 1229
Query: 214 FGNGKSIY 221
FGN K ++
Sbjct: 1230FGNAKVVF 1253
>TC87503 similar to SP|Q43111|PME3_PHAVU Pectinesterase 3 precursor (EC
3.1.1.11) (Pectin methylesterase 3) (PE 3)., partial
(93%)
Length = 2088
Score = 103 bits (258), Expect = 7e-23
Identities = 60/177 (33%), Positives = 94/177 (52%)
Frame = +2
Query: 45 TAAETTVRVVRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFV 104
T TV V KDG+G + T+ +A+K + + +R VV++ G Y E I +D++ V
Sbjct: 851 TVVNETVPDAVVAKDGSGQYKTIGEALKLVKKKSLQRFVVYVKKGVYVENIDLDKNTWNV 1030
Query: 105 TFYGERNGKDNDMMPIITYDATALRYGTLDSATVAVDADYFVAVNVAFVNSSPMPDENSV 164
YG+ + D T T ++AT AV F+A ++ F+N++
Sbjct: 1031 MIYGDGMTETVVSGSRNYIDGTP----TFETATFAVKGKGFIAKDIQFLNTAGASKH--- 1189
Query: 165 GGQALAMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIY 221
QA+AMR D++ FY C F+G+QDTL + F++DC I GT DFIFGN +++
Sbjct: 1190 --QAVAMRSGSDQSVFYRCSFVGYQDTLYAHSNRQFYRDCDITGTIDFIFGNAAAVF 1354
>TC76734 similar to PIR|T06468|T06468 pectinesterase (EC 3.1.1.11) precursor
- garden pea, complete
Length = 1981
Score = 100 bits (248), Expect = 1e-21
Identities = 62/167 (37%), Positives = 91/167 (54%), Gaps = 4/167 (2%)
Frame = +3
Query: 54 VRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGERNGK 113
V V KDG+G F TV +AV S P+ R V+++ G Y+E + + SK V G+ G
Sbjct: 765 VVVAKDGSGKFKTVAEAVASAPNKGTARYVIYVKKGIYKENVEIASSKTNVMLLGD--GM 938
Query: 114 DNDMMPIITYDATALRY----GTLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQAL 169
D T +L Y GT +ATVA D+F+A ++ F N++ P ++ QA+
Sbjct: 939 D------ATIITGSLNYVDGTGTFQTATVAAVGDWFIAQDIGFQNTAG-PQKH----QAV 1085
Query: 170 AMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGN 216
A+R+ D++ CK FQDTL + F++D FI GT DFIFG+
Sbjct: 1086ALRVGSDRSVINRCKIDAFQDTLYAHTNRQFYRDSFITGTIDFIFGD 1226
>TC76732 homologue to PIR|T06468|T06468 pectinesterase (EC 3.1.1.11)
precursor - garden pea, complete
Length = 1860
Score = 99.8 bits (247), Expect = 1e-21
Identities = 61/175 (34%), Positives = 94/175 (52%), Gaps = 2/175 (1%)
Frame = +2
Query: 54 VRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGERNGK 113
V V +DG+G F TV AV S P K R V+++ G Y+E I + + K V G+ G
Sbjct: 758 VVVAQDGSGKFKTVAQAVASAPDNGKTRYVIYVKKGTYKENIEIGKKKTNVMLVGD--GM 931
Query: 114 DNDMMP--IITYDATALRYGTLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQALAM 171
D ++ + D T T SATVA D F+A ++ F N++ P ++ QA+A+
Sbjct: 932 DATIITGSLNFIDGTT----TFKSATVAAVGDGFIAQDIRFQNTAG-PQKH----QAVAL 1084
Query: 172 RISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIYLRTTI 226
R+ D++ CK FQDTL + F++D +I GT DFIFGN ++ ++ +
Sbjct: 1085RVGADQSVINRCKIDAFQDTLYAHSNRQFYRDSYITGTVDFIFGNAAVVFQKSKL 1249
>TC77013 similar to GP|7025485|gb|AAF35897.1| pectin methylesterase isoform
alpha {Vigna radiata}, partial (94%)
Length = 1655
Score = 97.4 bits (241), Expect = 7e-21
Identities = 57/166 (34%), Positives = 89/166 (53%)
Frame = +2
Query: 56 VRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGERNGKDN 115
V KDGTG+F T+ +A+ + P+ + R V+ I G Y E + V + K + G+ G+
Sbjct: 107 VAKDGTGNFATIGEAIAAAPNSSATRFVIHIKAGAYFENVEVIKKKTNLMLVGDGIGQTV 286
Query: 116 DMMPIITYDATALRYGTLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQALAMRISG 175
D + T S+T AV D F+A + F NS+ P ++ QA+A+R
Sbjct: 287 VKASRNVVDG----WTTFQSSTFAVVGDKFIAKGITFENSAG-PSKH----QAVAVRNGA 439
Query: 176 DKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIY 221
D +AFY C F+ +QDTL + F+++C + GT DFIFGN ++
Sbjct: 440 DFSAFYQCSFVAYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAVVF 577
>TC91752 similar to GP|6630559|gb|AAF19578.1| putative pectinesterase
{Arabidopsis thaliana}, partial (34%)
Length = 691
Score = 92.4 bits (228), Expect = 2e-19
Identities = 54/161 (33%), Positives = 88/161 (54%)
Frame = +3
Query: 56 VRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGERNGKDN 115
V KDG+G + T++ A+K +P+ + +R V+++ G Y E + V+++K V G+
Sbjct: 129 VAKDGSGKYKTISAALKHVPNKSDKRTVIYVKKGIYYENVRVEKTKWNVMIIGDGMNVTI 308
Query: 116 DMMPIITYDATALRYGTLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQALAMRISG 175
+ D T T +AT AV F+A ++ F N++ P ++ QA+A+ S
Sbjct: 309 VSGSLNFIDGTP----TFSTATFAVFGRNFIARDIGFKNTAG-PQKH----QAVALMTSA 461
Query: 176 DKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGN 216
D+A FY C +QDTL + F+++C I GT DFIFGN
Sbjct: 462 DQAVFYKCSMDAYQDTLYAHSNRQFYRECNIYGTVDFIFGN 584
>TC77735 similar to GP|14334646|gb|AAK59501.1 putative pectinesterase
{Arabidopsis thaliana}, partial (59%)
Length = 1963
Score = 87.8 bits (216), Expect(2) = 2e-19
Identities = 57/177 (32%), Positives = 97/177 (54%), Gaps = 4/177 (2%)
Frame = +2
Query: 54 VRVRKDGTGDFTTVTDAVKSIPSGNKRR---VVVWIGMGEYREKITVDRSKRFVTFYGER 110
V V G + T++ DA+ + P+ K ++++ G Y E + V + K + G+
Sbjct: 827 VLVSPYGIANHTSIGDAIAAAPNNTKPEDGYYLIYVREGYYEEYVIVPKHKNNILLVGD- 1003
Query: 111 NGKDNDMMPIITYDATALR-YGTLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQAL 169
G +N IIT + + + + T +S+T AV + F+AV++ F N++ P+++ QA+
Sbjct: 1004-GINNT---IITGNHSVIDGWTTFNSSTFAVSGERFIAVDITFRNTAG-PEKH----QAV 1156
Query: 170 AMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIYLRTTI 226
A+R + D + FY C F G+QDTL + F++DC I GT DFIFGN ++ I
Sbjct: 1157AVRNNADLSTFYRCSFEGYQDTLYVHSVRQFYRDCKIYGTVDFIFGNAAVVFQNCNI 1327
Score = 25.0 bits (53), Expect(2) = 2e-19
Identities = 9/23 (39%), Positives = 16/23 (69%)
Frame = +1
Query: 227 ESVAKGFECHHSTR*GKNVGRHW 249
+++AK +C HS+R* + +HW
Sbjct: 1336 KTIAKSEKCCHSSR*N*SKSKHW 1404
>TC85623 similar to GP|13605696|gb|AAK32841.1 At2g45220/F4L23.27 {Arabidopsis
thaliana}, partial (79%)
Length = 2442
Score = 92.0 bits (227), Expect = 3e-19
Identities = 63/174 (36%), Positives = 93/174 (53%), Gaps = 2/174 (1%)
Frame = +3
Query: 45 TAAETTVRVVRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFV 104
T++ + V V KDG+G +TTV A + PSG+ R V+++ G Y E++ + V
Sbjct: 1152 TSSAASKANVVVAKDGSGKYTTVKAATDAAPSGSGR-YVIYVKAGVYNEQVEIKAKN--V 1322
Query: 105 TFYGERNGKDNDMMPIITYDATALRYGTLD--SATVAVDADYFVAVNVAFVNSSPMPDEN 162
G+ GK IIT + ++ GT SATVA D F+A ++ F N++
Sbjct: 1323 MLVGDGIGKT-----IIT-GSKSVGGGTTTFRSATVAATGDGFIAQDITFRNTA-----G 1469
Query: 163 SVGGQALAMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGN 216
+ QA+A R D + FY C F G+QDTL + F+++C I GT DFIFGN
Sbjct: 1470 AANHQAVAFRSGSDLSVFYKCSFEGYQDTLYVHSERQFYRECNIYGTVDFIFGN 1631
>TC76733 similar to PIR|T06468|T06468 pectinesterase (EC 3.1.1.11) precursor
- garden pea, partial (98%)
Length = 2029
Score = 92.0 bits (227), Expect = 3e-19
Identities = 56/170 (32%), Positives = 87/170 (50%), Gaps = 2/170 (1%)
Frame = +2
Query: 54 VRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGERNGK 113
V V +DG+G F TV +AV S P K + V+++ G Y+E + + K V G+ G
Sbjct: 773 VVVAQDGSGKFKTVAEAVASAPDNGKTKYVIYVKKGTYKENVEIGSKKTNVMLVGD--GM 946
Query: 114 DNDMMP--IITYDATALRYGTLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQALAM 171
D ++ + D T T S+TVA D F+A ++ F N + + QA+A+
Sbjct: 947 DATIITGNLNFIDGTT----TFKSSTVAAVGDGFIAQDIWFQNMA-----GAAKHQAVAL 1099
Query: 172 RISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIY 221
R+ D++ C+ FQDTL + F++D I GT DFIFGN ++
Sbjct: 1100RVGSDQSVINRCRIDAFQDTLYAHSNRQFYRDSVITGTIDFIFGNAAVVF 1249
>AL368659 similar to PIR|B86158|B86 hypothetical protein AAF02886.1
[imported] - Arabidopsis thaliana, partial (25%)
Length = 495
Score = 87.8 bits (216), Expect = 5e-18
Identities = 53/155 (34%), Positives = 87/155 (55%), Gaps = 4/155 (2%)
Frame = +1
Query: 58 KDGTGDFTTVTDAVKSIPSGNKRR---VVVWIGMGEYREKITVDRSKRFVTFYGERNGKD 114
+DG+G+FTT+ DA+ + P+ +++I G Y E +++D+ K+++ GE +
Sbjct: 61 QDGSGNFTTINDAIAAAPNNTVASDGYFLIFITEGVYEEYVSIDKKKKYLMMIGEGINQT 240
Query: 115 NDMMPIITYDAT-ALRYGTLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQALAMRI 173
IIT + A + T +SAT AV A FVAVN+ F N++ + QA+A+R
Sbjct: 241 -----IITGNRNVADGFTTFNSATFAVVAQGFVAVNITFRNTA-----GAAKNQAVALRS 390
Query: 174 SGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQG 208
D + FY+C F G+QDTL + F+++C I G
Sbjct: 391 GADMSTFYSCSFEGYQDTLYTHSLRQFYRECDIYG 495
>BE319906 similar to SP|Q43062|PME_ Pectinesterase PPE8B precursor (EC
3.1.1.11) (Pectin methylesterase) (PE). [Peach] {Prunus
persica}, partial (36%)
Length = 589
Score = 86.7 bits (213), Expect = 1e-17
Identities = 52/166 (31%), Positives = 85/166 (50%), Gaps = 4/166 (2%)
Frame = +2
Query: 60 GTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGERNGKDNDMMP 119
G+GD+ V DAV + P + +R V+++ G Y E + + + K + GE G D
Sbjct: 2 GSGDYAKVMDAVSAAPESSMKRYVIYVKKGVYVENVEIKKKKWNIMLIGE--GMD----- 160
Query: 120 IITYDATALRY----GTLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQALAMRISG 175
T + + Y T SAT AV F+A +++F N++ QA+A+R
Sbjct: 161 -ATIISGSRNYVDGSTTFRSATFAVSGRGFIARDISFQNTAGAEKH-----QAVALRSDS 322
Query: 176 DKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIY 221
D + FY C G+QD+L + F+++C I GT DFIFG+ +++
Sbjct: 323 DLSVFYRCGIFGYQDSLYTHTMRQFYRECKISGTVDFIFGDATAVF 460
>BQ138825 similar to PIR|T49922|T49 pectin methylesterase-like protein -
Arabidopsis thaliana, partial (36%)
Length = 658
Score = 86.7 bits (213), Expect = 1e-17
Identities = 59/157 (37%), Positives = 86/157 (54%), Gaps = 4/157 (2%)
Frame = +1
Query: 69 DAVKSIPSG--NKRRVVVWIGMGEYREKITVDRSKRFVTFYGERNGKDNDMMPIITYDAT 126
+AV + P +++R V++I G Y E + V KR V F G+ GK +IT A
Sbjct: 4 EAVNAAPDNGVDRKRFVIYIKEGVYEETVRVPLKKRNVVFLGDGIGKT-----VITGSAN 168
Query: 127 ALRYG--TLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQALAMRISGDKAAFYNCK 184
+ G T +SATVAV D F+A ++ N++ PD + QA+A R+ D + NC+
Sbjct: 169 VGQPGMTTYNSATVAVLGDGFMAKDLTIENTAG-PDAH----QAVAFRLDSDLSVIENCE 333
Query: 185 FIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIY 221
F+G QDTL + F+K C I G DFIFGN +I+
Sbjct: 334 FLGNQDTLYAHSLRQFYKSCRIVGNVDFIFGNSAAIF 444
>BI272853 weakly similar to PIR|T04359|T04 pectin methylesterase-like protein
- maize, partial (25%)
Length = 678
Score = 73.2 bits (178), Expect = 1e-13
Identities = 47/147 (31%), Positives = 72/147 (48%), Gaps = 1/147 (0%)
Frame = +1
Query: 81 RVVVWIGMGEYREKITVDRSKRFVTFYGERNGKDNDMMPIITYDAT-ALRYGTLDSATVA 139
R +++ G Y E IT+ + + YG+ GK I+T A T+ +AT A
Sbjct: 4 RYTIYVKAGVYDEYITIPKDAVNILMYGDGPGKT-----IVTGRKNGAAGVKTMQTATFA 168
Query: 140 VDADYFVAVNVAFVNSSPMPDENSVGGQALAMRISGDKAAFYNCKFIGFQDTLCDDYGKH 199
A F+ + F N++ G QA+A R GD +A C +G+QDTL +
Sbjct: 169 NTALGFIGKAMTFENTA-----GPAGHQAVAFRNQGDMSALVGCHILGYQDTLYVQTNRQ 333
Query: 200 FFKDCFIQGTYDFIFGNGKSIYLRTTI 226
F+++C I GT DFIFG ++ +TI
Sbjct: 334 FYRNCVISGTVDFIFGTSATLIQDSTI 414
>TC84419 similar to SP|Q42920|PME_MEDSA Pectinesterase precursor (EC
3.1.1.11) (Pectin methylesterase) (PE) (P65). [Alfalfa],
partial (53%)
Length = 732
Score = 72.8 bits (177), Expect = 2e-13
Identities = 33/78 (42%), Positives = 50/78 (63%)
Frame = +2
Query: 144 YFVAVNVAFVNSSPMPDENSVGGQALAMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKD 203
+F A+NV F NS+ + QA+A+R++ DKA FYNC+ G+QDTL + F++D
Sbjct: 83 HFTALNVGFENSA-----GAAKHQAVALRVTADKALFYNCQMNGYQDTLYTQSKRQFYRD 247
Query: 204 CFIQGTYDFIFGNGKSIY 221
C I GT DF+FG+ ++
Sbjct: 248 CTITGTIDFVFGDAVGVF 301
>TC92167 similar to PIR|T05203|T05203 pectinesterase homolog F4I10.160 -
Arabidopsis thaliana, partial (11%)
Length = 652
Score = 49.3 bits (116), Expect = 2e-06
Identities = 26/61 (42%), Positives = 36/61 (58%), Gaps = 1/61 (1%)
Frame = +1
Query: 165 GGQALAMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDC-FIQGTYDFIFGNGKSIYLR 223
G QA+A R+ D+A F NC+F GFQDTL + FF + +DFIFG+ +Y +
Sbjct: 244 GHQAVAARVQADRAVFANCRFEGFQDTLYTVAHRQFFP*LHHNREQFDFIFGDPSPLYSK 423
Query: 224 T 224
T
Sbjct: 424 T 426
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.328 0.141 0.457
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,056,333
Number of Sequences: 36976
Number of extensions: 206836
Number of successful extensions: 1318
Number of sequences better than 10.0: 53
Number of HSP's better than 10.0 without gapping: 1273
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1287
length of query: 335
length of database: 9,014,727
effective HSP length: 97
effective length of query: 238
effective length of database: 5,428,055
effective search space: 1291877090
effective search space used: 1291877090
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC124967.4