BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124966.6 + phase: 0
(176 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC77864 weakly similar to PIR|T06329|T06329 symbiotic ammonium t... 72 9e-14
TC84899 similar to GP|20127016|gb|AAM10935.1 putative bHLH trans... 71 2e-13
BE124317 weakly similar to GP|20127014|gb| putative bHLH transcr... 62 1e-10
TC92979 similar to GP|20127014|gb|AAM10934.1 putative bHLH trans... 58 2e-09
BM780173 similar to PIR|T06329|T063 symbiotic ammonium transport... 54 3e-08
TC89598 weakly similar to GP|20127014|gb|AAM10934.1 putative bHL... 53 7e-08
TC78996 similar to GP|20127022|gb|AAM10937.1 putative bHLH trans... 51 3e-07
TC91064 weakly similar to GP|20127014|gb|AAM10934.1 putative bHL... 50 4e-07
TC78413 similar to PIR|T06329|T06329 symbiotic ammonium transpor... 49 8e-07
TC79250 similar to PIR|T06329|T06329 symbiotic ammonium transpor... 47 4e-06
TC90345 similar to GP|20127014|gb|AAM10934.1 putative bHLH trans... 45 1e-05
BG449760 40 6e-04
BG648958 similar to GP|19310467|gb| At1g01260/F6F3_25 {Arabidops... 37 0.005
BG586877 similar to GP|16226919|gb AT4g16430/dl4240w {Arabidopsi... 36 0.009
BE323853 similar to GP|20127022|gb| putative bHLH transcription ... 33 0.045
TC88734 similar to GP|13346180|gb|AAK19612.1 GHDEL61 {Gossypium ... 33 0.076
TC80836 similar to GP|23499268|emb|CAD51348. hypothetical protei... 32 0.17
TC90528 homologue to GP|21592490|gb|AAM64440.1 unknown {Arabidop... 30 0.65
TC93549 similar to GP|20127044|gb|AAM10942.1 putative bHLH trans... 30 0.65
TC90648 similar to GP|20127044|gb|AAM10942.1 putative bHLH trans... 30 0.65
>TC77864 weakly similar to PIR|T06329|T06329 symbiotic ammonium transport
protein SAT1 - soybean, partial (21%)
Length = 1411
Score = 72.4 bits (176), Expect = 9e-14
Identities = 56/184 (30%), Positives = 90/184 (48%), Gaps = 27/184 (14%)
Frame = +3
Query: 11 DEDKFQREIVQHQP-----SFSFESGNSQSSTIQYMNNIVDNVCVTNTV----------- 54
DE +F ++I+ +P S S ++ ++ +N D TN++
Sbjct: 150 DEQEFLKDIILEEPECIELSSYLCSNKTKDNSTTIINVEGDATSPTNSILSFDETSLFCG 329
Query: 55 -HENIAQEYTWATNSQQLP---------PSQYILSFENSTMQPSPNSDIATCSSIMVQET 104
+EN+ + + NS + P+ Y+LSF NS+++P + E
Sbjct: 330 DYENVETNHK-SNNSNSIKSLERSCVSSPATYLLSFGNSSIEP-------------IIEP 467
Query: 105 TTLNNNVSSELPKIIKKRTKNLRSSCE-MQDHIMAERKRRQVLTERFIALSATIPGLKKV 163
+ ++ + +K+ TK +R SCE +QDH+MAERKRR+ LTE IALSA IPGLKK+
Sbjct: 468 MSHKTKRRTDESRGVKEATKKVRRSCETVQDHLMAERKRRRELTENIIALSAMIPGLKKM 647
Query: 164 SFLY 167
Y
Sbjct: 648 DKCY 659
>TC84899 similar to GP|20127016|gb|AAM10935.1 putative bHLH transcription
factor {Arabidopsis thaliana}, partial (5%)
Length = 580
Score = 71.2 bits (173), Expect = 2e-13
Identities = 55/167 (32%), Positives = 84/167 (49%), Gaps = 17/167 (10%)
Frame = +1
Query: 9 YLDEDKFQREIVQHQPSFSFESGNSQSSTI--------QYMNNIVDNVCVTNTV---HEN 57
Y D F ++I+ P S S+++++ +++ N++ + + V H+
Sbjct: 109 YFIGDDFLQDILLQTPPQSLMYSESENNSVTVNVNGGVEFVGNMIKSNSSNSIVSQQHQP 288
Query: 58 IAQEYTWATNSQQLP-----PSQYILSFENSTMQPS-PNSDIATCSSIMVQETTTLNNNV 111
QE S+++P P YILSF+NSTM P+ PN + + +N
Sbjct: 289 QEQEQEHGLKSKKVPRRSSSPKTYILSFDNSTMIPATPN----------YKNKRSHESNQ 438
Query: 112 SSELPKIIKKRTKNLRSSCEMQDHIMAERKRRQVLTERFIALSATIP 158
SE+ + K RSS + DHIMAERKRRQ L+E+FIALS TIP
Sbjct: 439 KSEMKINQQNGVKRGRSSSQCIDHIMAERKRRQELSEKFIALSPTIP 579
>BE124317 weakly similar to GP|20127014|gb| putative bHLH transcription
factor {Arabidopsis thaliana}, partial (10%)
Length = 384
Score = 62.0 bits (149), Expect = 1e-10
Identities = 41/95 (43%), Positives = 51/95 (53%), Gaps = 3/95 (3%)
Frame = +3
Query: 68 SQQLPPSQYILSFENSTMQPSPNSDIATCSSIMVQETTTLNNNVSSELP---KIIKKRTK 124
S P +ILSF ST+ P+ T S V T + +NN L K + K
Sbjct: 111 STPFTPKTFILSFNKSTIIPATT----TPQSQQVPTTNSRSNNNKRSLEPKAKASNQTGK 278
Query: 125 NLRSSCEMQDHIMAERKRRQVLTERFIALSATIPG 159
RS + DHIMAERKRR L+++FIALSATIPG
Sbjct: 279 KSRSGSQCLDHIMAERKRRLELSQKFIALSATIPG 383
>TC92979 similar to GP|20127014|gb|AAM10934.1 putative bHLH transcription
factor {Arabidopsis thaliana}, partial (15%)
Length = 744
Score = 58.2 bits (139), Expect = 2e-09
Identities = 49/146 (33%), Positives = 74/146 (50%), Gaps = 19/146 (13%)
Frame = +2
Query: 40 YMNNIVDNV--------CVT--NTVHENIAQEYTWATNSQQLPPS-QY-ILSFENSTMQP 87
+++N + NV C++ +T HE I N++ L PS Q+ + SF+N
Sbjct: 188 FLSNDISNVFQEQNKQQCLSLGSTFHETIDNS---DKNNESLSPSFQFQVPSFDNPP-NS 355
Query: 88 SPNSDIATCSSIMVQETTTLNNNVSSELPK-------IIKKRTKNLRSSCEMQDHIMAER 140
SP + +I + T N N S+E K + K +K+ R +DHIMAER
Sbjct: 356 SPTNSKENIETIPLSPTDLENMNHSTETSKGSLENKKLETKTSKSKRPRAHGRDHIMAER 535
Query: 141 KRRQVLTERFIALSATIPGLKKVSFL 166
RR+ LT+ FIAL+A +P LKK+ L
Sbjct: 536 NRREKLTQSFIALAALVPNLKKMDKL 613
>BM780173 similar to PIR|T06329|T063 symbiotic ammonium transport protein
SAT1 - soybean, partial (14%)
Length = 732
Score = 53.9 bits (128), Expect = 3e-08
Identities = 28/45 (62%), Positives = 36/45 (79%), Gaps = 2/45 (4%)
Frame = -3
Query: 134 DHIMAERKRRQVLTERFIALSATIPGLKKV--SFLYKPTISLIKL 176
DHIMAERKRR L+++FIALSATIPGLKK+ +++ IS +KL
Sbjct: 670 DHIMAERKRRLELSQKFIALSATIPGLKKMDKNYILGEAISYVKL 536
>TC89598 weakly similar to GP|20127014|gb|AAM10934.1 putative bHLH
transcription factor {Arabidopsis thaliana}, partial
(24%)
Length = 849
Score = 52.8 bits (125), Expect = 7e-08
Identities = 45/176 (25%), Positives = 77/176 (43%), Gaps = 15/176 (8%)
Frame = +3
Query: 3 YDKSHFYLDEDKFQREIVQHQPSFSFESGNSQSSTIQYMNNIVDNVCVTNTVHENIAQEY 62
+ + +D FQ + + + SFS S S+ +NN +H Q
Sbjct: 138 FSDHEYQMDSYAFQFDDMAYFKSFSESPQESTYSSHTNINN--------KRIHSESTQNS 293
Query: 63 TWATNSQQ------------LPPSQYILSFE---NSTMQPSPNSDIATCSSIMVQETTTL 107
++ T S L S I+SF+ N + P ++I ++ +
Sbjct: 294 SFPTQSPDQSVASATPPTKLLKASPKIISFDYSNNDSKVKKPKTEIGYGENLNFGSVISQ 473
Query: 108 NNNVSSELPKIIKKRTKNLRSSCEMQDHIMAERKRRQVLTERFIALSATIPGLKKV 163
+ E K + R+ + +DH++AERKRR+ L++RFIALS+ +PGLKK+
Sbjct: 474 GDYYKRE-----NKVSAVNRNPMQARDHVIAERKRREKLSQRFIALSSILPGLKKM 626
>TC78996 similar to GP|20127022|gb|AAM10937.1 putative bHLH transcription
factor {Arabidopsis thaliana}, partial (27%)
Length = 1081
Score = 50.8 bits (120), Expect = 3e-07
Identities = 22/37 (59%), Positives = 31/37 (83%)
Frame = +3
Query: 127 RSSCEMQDHIMAERKRRQVLTERFIALSATIPGLKKV 163
R++ + ++H+MAERKRR+ LT FIALSA +PGLKK+
Sbjct: 45 RNTAQAREHVMAERKRREKLTRSFIALSAIVPGLKKM 155
>TC91064 weakly similar to GP|20127014|gb|AAM10934.1 putative bHLH
transcription factor {Arabidopsis thaliana}, partial
(19%)
Length = 751
Score = 50.4 bits (119), Expect = 4e-07
Identities = 30/90 (33%), Positives = 52/90 (57%), Gaps = 1/90 (1%)
Frame = +2
Query: 82 NSTMQPSPNSDIATCSSIMVQETTTLNNNVS-SELPKIIKKRTKNLRSSCEMQDHIMAER 140
NS++ S +++ +S+ + T ++++ S +K R++ + DHIM+ER
Sbjct: 20 NSSINVSQHAEEMPTNSLSIPTTEQHHDSLPLSSSTANQGSNSKKPRNTSDTLDHIMSER 199
Query: 141 KRRQVLTERFIALSATIPGLKKVSFLYKPT 170
RRQ+LT + I LSA IPGLKK+ ++ T
Sbjct: 200 NRRQLLTSKIIELSALIPGLKKIDKVHVVT 289
>TC78413 similar to PIR|T06329|T06329 symbiotic ammonium transport protein
SAT1 - soybean, partial (30%)
Length = 1397
Score = 49.3 bits (116), Expect = 8e-07
Identities = 52/149 (34%), Positives = 69/149 (45%), Gaps = 1/149 (0%)
Frame = +2
Query: 16 QREIVQHQPSFSFESGNSQSSTIQYMNNIVDNVCVTNTVHENIAQEYTWATNSQQLPPSQ 75
Q E QHQP S ES SS+ N E ++ T+S SQ
Sbjct: 227 QEENFQHQPLLSSES---YSSSYPLEKNF-----------ERPNKKLKKNTSSPSSTTSQ 364
Query: 76 YILSFENSTMQPSPNSDIATCSSIMVQETT-TLNNNVSSELPKIIKKRTKNLRSSCEMQD 134
ILSF+ T+ N + + + Q +L E K + TK RS QD
Sbjct: 365 -ILSFD-CTLNTKKNKVVPLSQTELPQNRKGSLQKQNIVETIKPQGQGTK--RSVAHNQD 532
Query: 135 HIMAERKRRQVLTERFIALSATIPGLKKV 163
HI+AERKRR+ L++ IAL+A IPGLKK+
Sbjct: 533 HIIAERKRREKLSQCLIALAALIPGLKKM 619
>TC79250 similar to PIR|T06329|T06329 symbiotic ammonium transport protein
SAT1 - soybean, partial (34%)
Length = 1145
Score = 47.0 bits (110), Expect = 4e-06
Identities = 22/45 (48%), Positives = 34/45 (74%)
Frame = +2
Query: 119 IKKRTKNLRSSCEMQDHIMAERKRRQVLTERFIALSATIPGLKKV 163
I++ K ++ QDHI+AERKRR+ ++++FIALSA +P LKK+
Sbjct: 464 IQESKKTDSAARNAQDHIIAERKRREKISQKFIALSALLPDLKKM 598
>TC90345 similar to GP|20127014|gb|AAM10934.1 putative bHLH transcription
factor {Arabidopsis thaliana}, partial (21%)
Length = 906
Score = 45.1 bits (105), Expect = 1e-05
Identities = 31/93 (33%), Positives = 46/93 (49%), Gaps = 3/93 (3%)
Frame = +1
Query: 74 SQYILSFENSTMQPSPNSDIATCSSIMVQETT---TLNNNVSSELPKIIKKRTKNLRSSC 130
+ Y+ SFEN P + +V + NN S++ K T ++
Sbjct: 397 ASYVFSFENENPPPRKVEPALKPKTKVVNSKNGPRRVKNNESNKKNGSFSKST----TTH 564
Query: 131 EMQDHIMAERKRRQVLTERFIALSATIPGLKKV 163
DHI+AER RR+ +++ FIALSA IP LKK+
Sbjct: 565 HTPDHIIAERIRREKISQLFIALSALIPNLKKM 663
>BG449760
Length = 656
Score = 39.7 bits (91), Expect = 6e-04
Identities = 32/118 (27%), Positives = 50/118 (42%)
Frame = +2
Query: 37 TIQYMNNIVDNVCVTNTVHENIAQEYTWATNSQQLPPSQYILSFENSTMQPSPNSDIATC 96
TI M I + + T + + + S PP+ ++ Q P +
Sbjct: 335 TIMEMKTIDHSDKINETFTQKRSSSFQVQIPSFDSPPNS-----PTTSSQQYPTLNSIQN 499
Query: 97 SSIMVQETTTLNNNVSSELPKIIKKRTKNLRSSCEMQDHIMAERKRRQVLTERFIALS 154
+ V T N N S++ K + R N +DHIMA RKR++ LT+ FIAL+
Sbjct: 500 ERVSVSPTELENKNHSTKTSKTKRSRANN------GEDHIMAXRKRKEKLTQSFIALA 655
>BG648958 similar to GP|19310467|gb| At1g01260/F6F3_25 {Arabidopsis
thaliana}, partial (15%)
Length = 806
Score = 36.6 bits (83), Expect = 0.005
Identities = 17/44 (38%), Positives = 26/44 (58%)
Frame = +3
Query: 120 KKRTKNLRSSCEMQDHIMAERKRRQVLTERFIALSATIPGLKKV 163
K+ K E +H+ AER+RR+ L +RF AL A +P + K+
Sbjct: 288 KRGRKPANGRDEPLNHVEAERQRREKLNQRFYALRAVVPNISKM 419
>BG586877 similar to GP|16226919|gb AT4g16430/dl4240w {Arabidopsis thaliana},
partial (35%)
Length = 755
Score = 35.8 bits (81), Expect = 0.009
Identities = 21/59 (35%), Positives = 34/59 (57%), Gaps = 3/59 (5%)
Frame = +1
Query: 108 NNNVSSELPKIIKKRTKNLRSSC---EMQDHIMAERKRRQVLTERFIALSATIPGLKKV 163
N + SS L + K R + + + E +H+ AER+RR+ L +RF AL A +P + K+
Sbjct: 508 NEDSSSPLGEERKPRKRGRKPANGREEPLNHVEAERQRREKLNQRFYALRAGVPNISKM 684
>BE323853 similar to GP|20127022|gb| putative bHLH transcription factor
{Arabidopsis thaliana}, partial (7%)
Length = 409
Score = 33.5 bits (75), Expect = 0.045
Identities = 17/34 (50%), Positives = 22/34 (64%)
Frame = +3
Query: 138 AERKRRQVLTERFIALSATIPGLKKVSFLYKPTI 171
A KRR+ LT+ FIAL+A +P LKK+ Y I
Sbjct: 6 APGKRREKLTQNFIALAALVPNLKKLDEGYNMCI 107
>TC88734 similar to GP|13346180|gb|AAK19612.1 GHDEL61 {Gossypium hirsutum},
partial (40%)
Length = 1701
Score = 32.7 bits (73), Expect = 0.076
Identities = 13/32 (40%), Positives = 21/32 (65%)
Frame = +3
Query: 131 EMQDHIMAERKRRQVLTERFIALSATIPGLKK 162
E +H+++ER+RR L ERF+ L + +P K
Sbjct: 1395 EGMNHVLSERRRRAKLNERFLTLRSMVPSNSK 1490
>TC80836 similar to GP|23499268|emb|CAD51348. hypothetical protein
{Plasmodium falciparum 3D7}, partial (1%)
Length = 1193
Score = 31.6 bits (70), Expect = 0.17
Identities = 39/155 (25%), Positives = 63/155 (40%), Gaps = 12/155 (7%)
Frame = +1
Query: 18 EIVQHQPSFSFESGNSQSSTIQYMNNIVDNVCVTNTVHENIAQEY---TWATNSQQLPPS 74
E+V P+ S + S + N V + + H A E+ + T PS
Sbjct: 316 EVVADSPA----SSHGDSGFVADCRNEVQSSAAAHGSHPKPANEFRVCSLNTEDSVPSPS 483
Query: 75 QYILSFENSTMQPS---------PNSDIATCSSIMVQETTTLNNNVSSELPKIIKKRTKN 125
I ST+ P+ PNS SI V+E ++++S++ KR +
Sbjct: 484 DGIEDVNESTVVPNSVNVSAVVVPNSATPKPISI-VKEVGYPDSSISNQ------KRNEP 642
Query: 126 LRSSCEMQDHIMAERKRRQVLTERFIALSATIPGL 160
RS +DH+ A KR+ + + AL + PGL
Sbjct: 643 GRSLKSRKDHVAAVTKRKDIPSNFSSALQSVQPGL 747
>TC90528 homologue to GP|21592490|gb|AAM64440.1 unknown {Arabidopsis
thaliana}, partial (37%)
Length = 950
Score = 29.6 bits (65), Expect = 0.65
Identities = 13/29 (44%), Positives = 18/29 (61%)
Frame = +1
Query: 135 HIMAERKRRQVLTERFIALSATIPGLKKV 163
H +AER RR+ ++ER L +PG KV
Sbjct: 670 HSLAERARREKISERMKILQDLVPGCNKV 756
>TC93549 similar to GP|20127044|gb|AAM10942.1 putative bHLH transcription
factor {Arabidopsis thaliana}, partial (22%)
Length = 715
Score = 29.6 bits (65), Expect = 0.65
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +1
Query: 135 HIMAERKRRQVLTERFIALSATIPGLKKVS 164
H +AER RR+ ++ER L +PG KV+
Sbjct: 292 HSLAERVRREKISERMKFLQELVPGCSKVT 381
>TC90648 similar to GP|20127044|gb|AAM10942.1 putative bHLH transcription
factor {Arabidopsis thaliana}, partial (21%)
Length = 1164
Score = 29.6 bits (65), Expect = 0.65
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +3
Query: 135 HIMAERKRRQVLTERFIALSATIPGLKKVS 164
H +AER RR+ ++ER L +PG K++
Sbjct: 180 HSLAERVRREKISERMRLLQELVPGCNKIT 269
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.314 0.127 0.354
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,173,446
Number of Sequences: 36976
Number of extensions: 65907
Number of successful extensions: 469
Number of sequences better than 10.0: 63
Number of HSP's better than 10.0 without gapping: 468
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 468
length of query: 176
length of database: 9,014,727
effective HSP length: 90
effective length of query: 86
effective length of database: 5,686,887
effective search space: 489072282
effective search space used: 489072282
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 55 (25.8 bits)
Medicago: description of AC124966.6