BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124963.7 + phase: 0
(512 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC84227 weakly similar to GP|6682247|gb|AAF23299.1| unknown prot... 438 e-123
TC79587 similar to GP|6682247|gb|AAF23299.1| unknown protein {Ar... 184 9e-47
AL372616 weakly similar to GP|6682247|gb| unknown protein {Arabi... 159 2e-39
TC80956 weakly similar to GP|4510348|gb|AAD21437.1| expressed pr... 135 4e-32
AL372617 weakly similar to GP|6682247|gb|A unknown protein {Arab... 125 4e-29
TC80674 weakly similar to PIR|A86217|A86217 protein T23G18.18 [i... 109 3e-24
TC78096 weakly similar to GP|17529096|gb|AAL38758.1 unknown prot... 108 6e-24
TC78097 weakly similar to GP|17529096|gb|AAL38758.1 unknown prot... 103 1e-22
BI262788 similar to PIR|E96771|E96 unknown protein F1O17.3 [impo... 39 1e-08
TC92936 similar to GP|15292741|gb|AAK92739.1 unknown protein {Ar... 52 4e-07
TC80675 similar to GP|6714415|gb|AAF26103.1| hypothetical protei... 39 2e-06
BF004807 similar to PIR|A86217|A862 protein T23G18.18 [imported]... 45 9e-05
TC81980 weakly similar to GP|4510348|gb|AAD21437.1| expressed pr... 43 3e-04
TC78070 weakly similar to PIR|T07048|T07048 probable epoxide hyd... 40 0.002
TC86551 similar to SP|P48628|FD6C_SOYBN Omega-6 fatty acid desat... 28 8.4
BQ141661 weakly similar to GP|10177181|dbj gene_id:K19E20.3~unkn... 28 8.4
>TC84227 weakly similar to GP|6682247|gb|AAF23299.1| unknown protein
{Arabidopsis thaliana}, partial (34%)
Length = 629
Score = 438 bits (1126), Expect = e-123
Identities = 208/209 (99%), Positives = 208/209 (99%)
Frame = +3
Query: 221 SDPHPSRNFNSSAMDMLHLVDAVNVTDKFWVLCHSSGCIHAWASLKYIPERIAGAAMLAP 280
SDPHPSRNFNSSAMDMLHLVDAVNVTDKFWVLCHSSGCIHAWASLKYIPERIAGAAMLAP
Sbjct: 3 SDPHPSRNFNSSAMDMLHLVDAVNVTDKFWVLCHSSGCIHAWASLKYIPERIAGAAMLAP 182
Query: 281 MVSPYESHMTKDEMKRTWEKWLPRRKYMYSLAYRFPKLLSFFYRKSFLPEKHERIDKQFS 340
MVSPYESHMTKDEMKRTWEKWLPRRKYMYSLAYRFPKLLSFFYRKSFLPEKHERIDKQFS
Sbjct: 183 MVSPYESHMTKDEMKRTWEKWLPRRKYMYSLAYRFPKLLSFFYRKSFLPEKHERIDKQFS 362
Query: 341 LSLGKKDEILVDEPAFEEYWQRDLEESVRQGNLKPFIEEALLQVSRWDFNIEELHVHKKC 400
LSLGKKDEILVDEPAFEEYWQRDLEESVRQGNLKPFIEEALLQVSRWDFNIEELHVHKKC
Sbjct: 363 LSLGKKDEILVDEPAFEEYWQRDLEESVRQGNLKPFIEEALLQVSRWDFNIEELHVHKKC 542
Query: 401 QTGGLLLWLKSMYGQAECELAGYLGRIHI 429
QTGGLLLWLKSMYGQAECEL GYLGRIHI
Sbjct: 543 QTGGLLLWLKSMYGQAECELXGYLGRIHI 629
>TC79587 similar to GP|6682247|gb|AAF23299.1| unknown protein {Arabidopsis
thaliana}, partial (12%)
Length = 1048
Score = 184 bits (466), Expect = 9e-47
Identities = 86/86 (100%), Positives = 86/86 (100%)
Frame = +2
Query: 427 IHIWQGLDDRMVPPSMTEYIERVLPEAVIHKLPNEGHFSYFFFCDECHKQIFSTLFGTPQ 486
IHIWQGLDDRMVPPSMTEYIERVLPEAVIHKLPNEGHFSYFFFCDECHKQIFSTLFGTPQ
Sbjct: 2 IHIWQGLDDRMVPPSMTEYIERVLPEAVIHKLPNEGHFSYFFFCDECHKQIFSTLFGTPQ 181
Query: 487 GPFERQEETMLEKNTEDALVSVSDIE 512
GPFERQEETMLEKNTEDALVSVSDIE
Sbjct: 182 GPFERQEETMLEKNTEDALVSVSDIE 259
>AL372616 weakly similar to GP|6682247|gb| unknown protein {Arabidopsis
thaliana}, partial (21%)
Length = 445
Score = 159 bits (402), Expect = 2e-39
Identities = 80/148 (54%), Positives = 109/148 (73%), Gaps = 8/148 (5%)
Frame = +1
Query: 62 EILMELGRGCRDILQQNFFNEDSYLVQK-------LRGPCSKLSKRLSFLNDFLPEDRDP 114
E+ +E +GCRDI++Q+ NEDS +V+K +R PC KL +LS+ N++LPED+DP
Sbjct: 1 ELTVEFLKGCRDIVKQSLVNEDSIIVKKFGKIGKRVRKPCEKLLNKLSYFNEYLPEDKDP 180
Query: 115 LLAWSIVFTVFLLAFAAISVDSNH-QTSTKAAMVRMHPPIASRILLPDGRYMAYQDQGVP 173
L AW ++ V LLAF+A+ V+ H Q++ V +HP A+RI+LPDGRYMAY++QGV
Sbjct: 181 LHAWFVILFVSLLAFSALYVNFEHDQSAPLEKKVFLHPVSATRIMLPDGRYMAYKEQGVS 360
Query: 174 PGRARFSLVAPHSFLSSRLAGIPGVKAS 201
RARFS++APH+FLSSRLAGIPGVK S
Sbjct: 361 ADRARFSIIAPHTFLSSRLAGIPGVKDS 444
>TC80956 weakly similar to GP|4510348|gb|AAD21437.1| expressed protein
{Arabidopsis thaliana}, partial (78%)
Length = 1219
Score = 135 bits (340), Expect = 4e-32
Identities = 90/316 (28%), Positives = 157/316 (49%), Gaps = 3/316 (0%)
Frame = +2
Query: 151 PPI-ASRILLPDGRYMAYQDQGVPPGRARFSLVAPHSFLSSRLAGI--PGVKASLLEDYG 207
PPI A RI L DGR++AY++ GVP A +V H F SSR + + LLE+ G
Sbjct: 137 PPITAPRIKLRDGRHLAYKEHGVPRQLASKKIVFLHGFGSSRHDAVIATNLPKGLLEELG 316
Query: 208 VRLVTYDLPGFGESDPHPSRNFNSSAMDMLHLVDAVNVTDKFWVLCHSSGCIHAWASLKY 267
V +V+ D PG+GESD P R+ S A+D+ L D + + KF+V+ S G W LK+
Sbjct: 317 VYVVSIDRPGYGESDADPKRSVKSLALDIEELADKLELGSKFYVMGFSMGGQAVWGCLKF 496
Query: 268 IPERIAGAAMLAPMVSPYESHMTKDEMKRTWEKWLPRRKYMYSLAYRFPKLLSFFYRKSF 327
IP R+AGA ++ P+V+ + + + + + + + ++ +A+ P L +++ +++
Sbjct: 497 IPHRLAGATLMTPVVNYWWHGLPYNMSTQAYYEQPIQDQWALRVAHYVPWLTFWWFTQNW 676
Query: 328 LPEKHERIDKQFSLSLGKKDEILVDEPAFEEYWQRDLEESVRQGNLKPFIEEALLQVSRW 387
P R+ K S L +D +V + + + +++ QG + + ++ W
Sbjct: 677 FPT--SRVVKGNSAVLSPQDLSIVFNMSVNKEHRSQMKQ---QGEHESICRDTIIGFGTW 841
Query: 388 DFNIEELHVHKKCQTGGLLLWLKSMYGQAECELAGYLGRIHIWQGLDDRMVPPSMTEYIE 447
DF+ +L + + ++H+WQG DD++VP ++ YI
Sbjct: 842 DFDPLDL----------------------DNPFSDNTIKVHLWQGADDKLVPATLQRYIV 955
Query: 448 RVLPEAVIHKLPNEGH 463
+ LP H+L GH
Sbjct: 956 QKLPWIQYHELQGAGH 1003
>AL372617 weakly similar to GP|6682247|gb|A unknown protein {Arabidopsis
thaliana}, partial (12%)
Length = 481
Score = 125 bits (314), Expect = 4e-29
Identities = 54/94 (57%), Positives = 70/94 (74%)
Frame = +1
Query: 419 ELAGYLGRIHIWQGLDDRMVPPSMTEYIERVLPEAVIHKLPNEGHFSYFFFCDECHKQIF 478
E G+LG IHIWQG+DD++VPPSMT+++ RVLP A +HKLP EGHF+Y +FCDECH+QIF
Sbjct: 10 EYTGFLGPIHIWQGMDDKVVPPSMTDFVHRVLPGAAVHKLPYEGHFTYIYFCDECHRQIF 189
Query: 479 STLFGTPQGPFERQEETMLEKNTEDALVSVSDIE 512
+TLFGTPQGP ++ T +A V + E
Sbjct: 190 TTLFGTPQGPLSLDISLEVDPITLEANVELEQQE 291
>TC80674 weakly similar to PIR|A86217|A86217 protein T23G18.18 [imported] -
Arabidopsis thaliana, partial (35%)
Length = 848
Score = 109 bits (272), Expect = 3e-24
Identities = 58/171 (33%), Positives = 98/171 (56%), Gaps = 4/171 (2%)
Frame = +1
Query: 153 IASRILLPDGRYMAYQDQGVPPGRARFSLVAPHSFLSSRLAGIPGVKASLLEDYGVRLVT 212
++ RI L DGR++AY ++GVP +A + ++ H F SS+ + L+++ G+ L+
Sbjct: 220 VSPRIRLRDGRHLAYVERGVPKDKATYKIIIVHGFGSSKEMNFLAPQ-ELIDELGIYLLQ 396
Query: 213 YDLPGFGESDPHPSRNFNSSAMDMLHLVDAVNVTDKFWVLCHSSGCIHAWASLKYIPERI 272
YD G+GESDP+P R+ S A+D+ L D + + F+V+ S G W+ LKY+P R+
Sbjct: 397 YDRAGYGESDPNPKRSLKSEALDIEELADQLQIGANFYVIGVSMGSYATWSCLKYLPHRL 576
Query: 273 AGAAMLAPMVS----PYESHMTKDEMKRTWEKWLPRRKYMYSLAYRFPKLL 319
AG A++AP+++ + +++ +R + KW LA PKLL
Sbjct: 577 AGLALIAPVINYRWPSLPGSLIREDYRRRFIKW------ALWLANHCPKLL 711
>TC78096 weakly similar to GP|17529096|gb|AAL38758.1 unknown protein
{Arabidopsis thaliana}, partial (78%)
Length = 1383
Score = 108 bits (269), Expect = 6e-24
Identities = 57/136 (41%), Positives = 85/136 (61%), Gaps = 3/136 (2%)
Frame = +1
Query: 151 PPI-ASRILLPDGRYMAYQDQGVPPGRARFSLVAPHSFLSSRLAGIPGVKAS--LLEDYG 207
PPI A RI L DGR++AY++ GVP A++ ++ H F S R + S +++ G
Sbjct: 286 PPITAPRIKLRDGRHLAYKEHGVPKDTAKYKIIYVHGFSSCRHDAVVAKTLSPDFVKELG 465
Query: 208 VRLVTYDLPGFGESDPHPSRNFNSSAMDMLHLVDAVNVTDKFWVLCHSSGCIHAWASLKY 267
V +V++D PG+GESDP P+R S A+D+ L D + + KF+V+ S G W LK+
Sbjct: 466 VYIVSFDRPGYGESDPDPNRTLKSIALDIEELADQLELGSKFYVIGTSLGGQIVWNCLKH 645
Query: 268 IPERIAGAAMLAPMVS 283
IP R+ GAA+LAP+V+
Sbjct: 646 IPHRLVGAALLAPVVN 693
Score = 42.4 bits (98), Expect = 4e-04
Identities = 22/59 (37%), Positives = 30/59 (50%)
Frame = +2
Query: 425 GRIHIWQGLDDRMVPPSMTEYIERVLPEAVIHKLPNEGHFSYFFFCDECHKQIFSTLFG 483
G +H+WQG +D MVP ++ YI + LP H+L GH F D + I L G
Sbjct: 1040 GSVHLWQGDEDIMVPVTLQRYIAQNLPWVHYHELSGSGHL--FPHADGVSETIIKLLLG 1210
>TC78097 weakly similar to GP|17529096|gb|AAL38758.1 unknown protein
{Arabidopsis thaliana}, partial (60%)
Length = 1070
Score = 103 bits (258), Expect = 1e-22
Identities = 58/182 (31%), Positives = 102/182 (55%), Gaps = 3/182 (1%)
Frame = +3
Query: 151 PPI-ASRILLPDGRYMAYQDQGVPPGRARFSLVAPHSFLSSRLAGIPG--VKASLLEDYG 207
PPI A RI L DGR++AY++ GV A++ ++ H + R + + ++++ G
Sbjct: 273 PPITAPRIKLRDGRHLAYKEHGVSKDVAKYKIIFVHGISTCRHDAVVANTLSPDVVKELG 452
Query: 208 VRLVTYDLPGFGESDPHPSRNFNSSAMDMLHLVDAVNVTDKFWVLCHSSGCIHAWASLKY 267
V +V++D PG+GESDP P+R S A+D+ L D + + KF+V+ S G W LK+
Sbjct: 453 VYIVSFDRPGYGESDPDPNRTLKSIALDIEELADQLGLGSKFYVIGFSMGGQIVWNCLKH 632
Query: 268 IPERIAGAAMLAPMVSPYESHMTKDEMKRTWEKWLPRRKYMYSLAYRFPKLLSFFYRKSF 327
IP R+AGAA+LAP+V+ + + + + + ++ +A+ P L ++ + +
Sbjct: 633 IPHRLAGAALLAPVVNYWWPDLPANLTAEAYSQMKMHDQWALRVAHYTPWLTYWWNTQRW 812
Query: 328 LP 329
P
Sbjct: 813 FP 818
>BI262788 similar to PIR|E96771|E96 unknown protein F1O17.3 [imported] -
Arabidopsis thaliana, partial (28%)
Length = 484
Score = 39.3 bits (90), Expect(2) = 1e-08
Identities = 22/42 (52%), Positives = 27/42 (63%), Gaps = 1/42 (2%)
Frame = +2
Query: 151 PPI-ASRILLPDGRYMAYQDQGVPPGRARFSLVAPHSFLSSR 191
PPI A RI L DGR++AY++ GVP A +V H F SSR
Sbjct: 122 PPITAPRIKLRDGRHLAYKEHGVPRQLASXKIVFLHGFGSSR 247
Score = 38.1 bits (87), Expect(2) = 1e-08
Identities = 18/38 (47%), Positives = 25/38 (65%)
Frame = +1
Query: 202 LLEDYGVRLVTYDLPGFGESDPHPSRNFNSSAMDMLHL 239
LLE+ GV +V+ D P +GESD P R+ S A+D+ L
Sbjct: 361 LLEELGVYVVSIDRPXYGESDADPKRSVKSLALDIEEL 474
>TC92936 similar to GP|15292741|gb|AAK92739.1 unknown protein {Arabidopsis
thaliana}, partial (41%)
Length = 762
Score = 52.4 bits (124), Expect = 4e-07
Identities = 40/136 (29%), Positives = 71/136 (51%), Gaps = 3/136 (2%)
Frame = +1
Query: 151 PPIAS-RILLPDGRYMAYQDQGVPPGRARFSLVAPHSFLSSRLAGIPGVKASLLEDYGVR 209
PP+ S R+ L DGR++AY++ G AR+ ++ H + +S+ +P V L++D G+
Sbjct: 283 PPVTSPRVKLSDGRHLAYREFGFSKEEARYKIIVIHGYANSKDTHLP-VSQELIDDLGIY 459
Query: 210 LVTYDLPGFGESDPHPSRNFNSSAMDMLHLVDAVNVTDKFWVLCHSS--GCIHAWASLKY 267
+ +D G+G + S F S + + + + D +L H + GC+ K
Sbjct: 460 FLHFDRAGYG-*E*SISITF-S*E*SI*YSRTSR*IADWEKILYHWNVIGCLLCLELPKN 633
Query: 268 IPERIAGAAMLAPMVS 283
IP R+ GAA++ P V+
Sbjct: 634 IPHRLLGAALVVPFVN 681
>TC80675 similar to GP|6714415|gb|AAF26103.1| hypothetical protein
{Arabidopsis thaliana}, partial (18%)
Length = 450
Score = 39.3 bits (90), Expect(2) = 2e-06
Identities = 16/39 (41%), Positives = 27/39 (69%)
Frame = +1
Query: 153 IASRILLPDGRYMAYQDQGVPPGRARFSLVAPHSFLSSR 191
++ RI L DGR++AY ++GVP +A + ++ H F SS+
Sbjct: 226 VSPRIRLRDGRHLAYVERGVPKDKATYKIIIVHGFGSSK 342
Score = 30.4 bits (67), Expect(2) = 2e-06
Identities = 11/22 (50%), Positives = 17/22 (77%)
Frame = +3
Query: 202 LLEDYGVRLVTYDLPGFGESDP 223
L+++ G+ L+ YD G+GESDP
Sbjct: 384 LIDELGIYLLQYDRAGYGESDP 449
>BF004807 similar to PIR|A86217|A862 protein T23G18.18 [imported] -
Arabidopsis thaliana, partial (15%)
Length = 521
Score = 44.7 bits (104), Expect = 9e-05
Identities = 21/62 (33%), Positives = 38/62 (60%)
Frame = +3
Query: 153 IASRILLPDGRYMAYQDQGVPPGRARFSLVAPHSFLSSRLAGIPGVKASLLEDYGVRLVT 212
+++RI L DGR++AY++ GV +A+ S++ H F SS+ L+++ GV ++
Sbjct: 333 VSTRIQLSDGRFLAYRESGVTKDKAKHSIIVVHGFGSSKDMNF-AAPQELIDELGVYILH 509
Query: 213 YD 214
YD
Sbjct: 510 YD 515
>TC81980 weakly similar to GP|4510348|gb|AAD21437.1| expressed protein
{Arabidopsis thaliana}, partial (25%)
Length = 684
Score = 43.1 bits (100), Expect = 3e-04
Identities = 22/59 (37%), Positives = 31/59 (52%)
Frame = +1
Query: 425 GRIHIWQGLDDRMVPPSMTEYIERVLPEAVIHKLPNEGHFSYFFFCDECHKQIFSTLFG 483
G +H+W G +D MVP ++ YI + LP H+LP GH F D + I +L G
Sbjct: 232 GSVHLWHGDEDIMVPVTLQRYIAQNLPWIHYHELPGSGHL--FPNADGVSETIIKSLLG 402
>TC78070 weakly similar to PIR|T07048|T07048 probable epoxide hydrolase (EC
3.3.2.3) (clone EH10.1) - potato, partial (95%)
Length = 1283
Score = 40.4 bits (93), Expect = 0.002
Identities = 27/83 (32%), Positives = 44/83 (52%), Gaps = 4/83 (4%)
Frame = +3
Query: 206 YGVRLVTYDLPGFGESD-PHPSRNFNSSAM--DMLHLVDAV-NVTDKFWVLCHSSGCIHA 261
+G R V DL G+G++D P ++ S + D++ L+DA+ +K +V+ H G + A
Sbjct: 189 HGYRCVAPDLRGYGDTDIPSSPTSYTSLHVVGDIIGLLDAIAGDQEKVFVVGHDWGALTA 368
Query: 262 WASLKYIPERIAGAAMLAPMVSP 284
W Y PERI L+ +P
Sbjct: 369 WYLSLYRPERIKALVNLSVAFTP 437
>TC86551 similar to SP|P48628|FD6C_SOYBN Omega-6 fatty acid desaturase
chloroplast precursor (EC 1.14.99.-). [Soybean] {Glycine
max}, partial (84%)
Length = 1629
Score = 28.1 bits (61), Expect = 8.4
Identities = 18/62 (29%), Positives = 24/62 (38%), Gaps = 7/62 (11%)
Frame = +3
Query: 249 FWVLCHSSGCIHAWASLKYIPERIAGAAMLAPMVSPYE-------SHMTKDEMKRTWEKW 301
F+V+ H C H S + E I G P++ PYE H K M + W
Sbjct: 522 FFVIGHD--CAHKSFSTNKLVEDIVGTLAFLPLIYPYEPWRFKHDKHHAKTNMLKEDTAW 695
Query: 302 LP 303
P
Sbjct: 696 QP 701
>BQ141661 weakly similar to GP|10177181|dbj gene_id:K19E20.3~unknown protein
{Arabidopsis thaliana}, partial (14%)
Length = 914
Score = 28.1 bits (61), Expect = 8.4
Identities = 25/93 (26%), Positives = 40/93 (42%), Gaps = 11/93 (11%)
Frame = +1
Query: 108 LPEDRDPLLAWSIVFTVFLLAFAAISVDSN----HQTSTKAA-------MVRMHPPIASR 156
LP P + + FT + F ++ D++ HQ S+ PP S
Sbjct: 241 LPPPHRPYSLFLL*FTQIIHIFNPVTPDTSQPHTHQQSSHVFPSPPSLFSFSRFPPHPSL 420
Query: 157 ILLPDGRYMAYQDQGVPPGRARFSLVAPHSFLS 189
+LPD Y ++ PP F+L+AP+ +LS
Sbjct: 421 FILPD--YSSHP----PPATRHFALIAPNIYLS 501
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.136 0.418
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,771,302
Number of Sequences: 36976
Number of extensions: 275724
Number of successful extensions: 1388
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 1374
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1381
length of query: 512
length of database: 9,014,727
effective HSP length: 100
effective length of query: 412
effective length of database: 5,317,127
effective search space: 2190656324
effective search space used: 2190656324
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Medicago: description of AC124963.7