BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124963.21 + phase: 0 /pseudo
(739 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imp... 90 4e-18
BG586266 similar to GP|7267666|em RNA-directed DNA polymerase-li... 87 2e-17
BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgari... 76 5e-14
TC80819 weakly similar to GP|10140689|gb|AAG13524.1 putative non... 65 1e-10
TC90516 58 2e-08
BG586267 weakly similar to PIR|A84888|A8 hypothetical protein At... 51 2e-06
TC77455 similar to GP|22335695|dbj|BAC10549. nine-cis-epoxycarot... 48 1e-05
BG447966 weakly similar to PIR|G96509|G96 protein F27F5.21 [impo... 44 3e-04
TC82948 42 7e-04
BG587108 similar to PIR|G96509|G9 protein F27F5.21 [imported] - ... 39 0.007
TC82763 37 0.027
TC85251 homologue to PIR|JQ2128|JQ2128 metallothionein - soybean... 33 0.40
BG647376 similar to GP|19807725|gb| CAMP-REGULATED GUANINE NUCLE... 33 0.52
TC81596 similar to GP|21039012|dbj|BAB92985. protein kinase {Ara... 32 0.68
AJ497561 weakly similar to GP|10140689|gb putative non-LTR retro... 32 0.88
BQ148771 31 1.5
BI308850 homologue to GP|13591616|db UDP-D-glucuronate carboxy-l... 30 2.6
TC77141 similar to GP|14164407|dbj|BAB55806. cytosolic aldehyde ... 30 3.4
TC79091 similar to GP|9294651|dbj|BAB03000.1 nucleotide sugar ep... 30 3.4
TC77606 similar to SP|O65729|RL18_CICAR 60S ribosomal protein L1... 28 9.8
>TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imported] -
Arabidopsis thaliana, partial (6%)
Length = 951
Score = 89.7 bits (221), Expect = 4e-18
Identities = 60/135 (44%), Positives = 79/135 (58%), Gaps = 11/135 (8%)
Frame = +2
Query: 70 RRLNDFCLISLVESMYKILAKVLANRLRYVIGMVISDSQSTFVKGRQILDGILVANEVVD 129
+RLNDF ISLV S+YKIL K+LANRLR VIG VISD+QS FVK RQIL+ + +
Sbjct: 95 QRLNDFRPISLVGSLYKILGKLLANRLRVVIGSVISDAQSAFVKNRQILEMVFL------ 256
Query: 130 DAHRCKKELLLFKVDFEEAYDIVDWSYLDEVMYKMG-----------FPVL*QK*IKECI 178
+ L + + + + + W + +G F VL +K IKEC+
Sbjct: 257 ----*QMRLWMRLRN*RKIFCCLRWILKRLITLSIGLIWILF*VGMSFLVLWRKWIKECV 424
Query: 179 GTATTSMLVNGSPTD 193
TATTS+LVNGSPT+
Sbjct: 425 STATTSVLVNGSPTN 469
>BG586266 similar to GP|7267666|em RNA-directed DNA polymerase-like protein
{Arabidopsis thaliana}, partial (18%)
Length = 789
Score = 87.4 bits (215), Expect = 2e-17
Identities = 46/137 (33%), Positives = 82/137 (59%), Gaps = 3/137 (2%)
Frame = -3
Query: 69 ARRLNDFCLISLVESMYKILAKVLANRLRYVIGMVISDSQSTFVKGRQILDGILVANEVV 128
A+R++++ I+ + YKI+AK+L+ R++ ++ +IS SQS FV GR I D +L+ ++++
Sbjct: 787 AKRVSEYRTIAPCNTQYKIIAKILSKRMQPLLRSIISPSQSAFVPGRAISDNVLITHKIL 608
Query: 129 DDAHR--CKKEL-LLFKVDFEEAYDIVDWSYLDEVMYKMGFPVL*QK*IKECIGTATTSM 185
+ KK + + K D +AYD + W++L EV+ ++GF + I EC+ T + S
Sbjct: 607 HYLRQSGAKKHVSMAVKTDMTKAYDRIAWNFLREVLTRLGFHGIWISWIMECVSTVSYSF 428
Query: 186 LVNGSPTDEFPLKRGCK 202
L+NG P RG +
Sbjct: 427 LINGGPQGRVLPSRGLR 377
>BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgaris}, partial
(69%)
Length = 616
Score = 75.9 bits (185), Expect = 5e-14
Identities = 44/152 (28%), Positives = 84/152 (54%), Gaps = 2/152 (1%)
Frame = +3
Query: 43 LVNFIGMGGLPKE-SIALSLLLFLRLRARRLNDFCLISLVESMYKILAKVLANRLRYVIG 101
+++F G +PK + LL + + +F I+ +YKI++K+L +R++ V+
Sbjct: 153 ILDFFKTGFMPKIINCTYVTLLPKEVNVTSVKNFRPIACCSVIYKIISKILTSRMQGVLN 332
Query: 102 MVISDSQSTFVKGRQILDGILVANEVVDD-AHRCKKELLLFKVDFEEAYDIVDWSYLDEV 160
V+S++QS FVKGR I D I++++E+V + + + K+D +AYD +W ++ +
Sbjct: 333 SVVSENQSAFVKGRVIFDNIILSHELVKSYSRKGISPRCMVKIDLXKAYDSXEWPFIKHL 512
Query: 161 MYKMGFPVL*QK*IKECIGTATTSMLVNGSPT 192
M ++GFP + + TA+ + NG T
Sbjct: 513 MLELGFPYKFVNWVMAXLTTASYTFNXNGDLT 608
>TC80819 weakly similar to GP|10140689|gb|AAG13524.1 putative non-LTR
retroelement reverse transcriptase {Oryza sativa
(japonica cultivar-group)}, partial (2%)
Length = 1262
Score = 64.7 bits (156), Expect = 1e-10
Identities = 30/48 (62%), Positives = 35/48 (72%), Gaps = 7/48 (14%)
Frame = +1
Query: 429 FNSALLGKWCWQLLIEKESLWYRVLSARYNEEGG-------RLLDGGR 469
FN +LLGKWCW+LL++KE LW+RVL ARY EEGG LL GGR
Sbjct: 49 FNLSLLGKWCWRLLVDKEGLWHRVLKARYGEEGGGFVRVVDSLLCGGR 192
Score = 30.8 bits (68), Expect = 2.0
Identities = 15/44 (34%), Positives = 23/44 (52%)
Frame = +3
Query: 461 GGRLLDGGRSTSAWWRVIATLQREEWFNSCREIGGEWERNILLV 504
GG L +GGR +S WW+ I ++ +G +E NI +V
Sbjct: 144 GGWLCEGGRQSSMWWKTICKVRE----GVGEGVGNWFEENIRMV 263
>TC90516
Length = 983
Score = 57.8 bits (138), Expect = 2e-08
Identities = 27/57 (47%), Positives = 43/57 (75%)
Frame = -2
Query: 72 LNDFCLISLVESMYKILAKVLANRLRYVIGMVISDSQSTFVKGRQILDGILVANEVV 128
L DF L+S + S+ K++AKVLA RL +++ +I +QSTFV+GRQ +DG++ NE++
Sbjct: 301 LGDFSLMSFLGSL*KLMAKVLALRLAHIMEKIIFVNQSTFVRGRQHVDGVVAINEII 131
>BG586267 weakly similar to PIR|A84888|A8 hypothetical protein At2g45230
[imported] - Arabidopsis thaliana, partial (16%)
Length = 794
Score = 50.8 bits (120), Expect = 2e-06
Identities = 38/119 (31%), Positives = 63/119 (52%), Gaps = 1/119 (0%)
Frame = +1
Query: 8 QCGTVIIINIRALMVLFLASLKIFGIFCVIMLCVLLVNFIGMGGLPKESIA-LSLLLFLR 66
QC + +A +S FG ++ + N G L +ES S L+ +
Sbjct: 436 QCSISTHTSAQARTA*MCSSFNNFGTQWEMISHLWRKNSSEQGNLKRESTKQTSGLVPKK 615
Query: 67 LRARRLNDFCLISLVESMYKILAKVLANRLRYVIGMVISDSQSTFVKGRQILDGILVAN 125
L A+RL +F ISL YKI++KVL+ RL+ V+ +I+++Q+ F + + I D IL+A+
Sbjct: 616 LEAKRLVEFRPISLCNVAYKIVSKVLSKRLKSVLPWIITETQAAFGRRQLISDNILIAH 792
>TC77455 similar to GP|22335695|dbj|BAC10549. nine-cis-epoxycarotenoid
dioxygenase1 {Pisum sativum}, partial (43%)
Length = 1865
Score = 48.1 bits (113), Expect = 1e-05
Identities = 31/76 (40%), Positives = 38/76 (49%), Gaps = 12/76 (15%)
Frame = -3
Query: 413 CLSKEVGGYGVRRIREFNSALLGKWCWQLLIEKESLWYRVLSARYNEEG----------G 462
CL + GG GVR IR N +LL KW W+LL ++ SLW VL Y G
Sbjct: 969 CLPRCKGGLGVRDIRLVNVSLLAKWWWRLLQDQSSLWKEVLEDIYGPRVSSRTMMVGRIG 790
Query: 463 RLL--DGGRSTSAWWR 476
RL+ DGG+ W R
Sbjct: 789 RLMLQDGGKI**VWRR 742
>BG447966 weakly similar to PIR|G96509|G96 protein F27F5.21 [imported] -
Arabidopsis thaliana, partial (7%)
Length = 687
Score = 43.5 bits (101), Expect = 3e-04
Identities = 23/51 (45%), Positives = 32/51 (62%)
Frame = +2
Query: 74 DFCLISLVESMYKILAKVLANRLRYVIGMVISDSQSTFVKGRQILDGILVA 124
D+ ISL + KI+ KV+ANR++ + VI QS FV+GR I D L+A
Sbjct: 482 DYRPISLCNVVMKIITKVIANRVKQTLPDVIDVEQSAFVQGRLITDNALIA 634
>TC82948
Length = 705
Score = 42.4 bits (98), Expect = 7e-04
Identities = 20/48 (41%), Positives = 26/48 (53%)
Frame = +3
Query: 403 KVTWVDWNFVCLSKEVGGYGVRRIREFNSALLGKWCWQLLIEKESLWY 450
KV V W VC + G G+R + + N AL K CW ++I KE WY
Sbjct: 255 KVVKVSWEKVCRPIKEGSLGIRSLSKLNEALNLKLCWDMMISKEQ-WY 395
>BG587108 similar to PIR|G96509|G9 protein F27F5.21 [imported] - Arabidopsis
thaliana, partial (17%)
Length = 677
Score = 38.9 bits (89), Expect = 0.007
Identities = 25/77 (32%), Positives = 41/77 (52%), Gaps = 1/77 (1%)
Frame = +3
Query: 39 LCVLLVNFIGMGGLPKESIALSLLLF-LRLRARRLNDFCLISLVESMYKILAKVLANRLR 97
L ++ +F+ G L ++ L + R R+ + ISL YKI++KVL RL+
Sbjct: 447 LLKMVNSFLASGKLDTRLNTTNICLIPKKKRPTRMTELRPISLCNVGYKIISKVLCQRLK 626
Query: 98 YVIGMVISDSQSTFVKG 114
+ +IS++QS FV G
Sbjct: 627 VCLPSLISETQSAFVHG 677
>TC82763
Length = 1376
Score = 37.0 bits (84), Expect = 0.027
Identities = 31/99 (31%), Positives = 46/99 (46%), Gaps = 8/99 (8%)
Frame = +1
Query: 409 WNFVCLSKEVGGYGVRRIREFNSALLGKWCWQLLIEKESLWYRVLSARYNEEGGRLLDGG 468
WN++C GG +R +R FN A+ W L E S + VL +Y G L+G
Sbjct: 244 WNWICRPIR-GGIRIRNLRFFNIAVR**-SWTLKTETNSFHFMVLFHKY----GSWLEGV 405
Query: 469 R-STSAWWRVIATLQRE-----EWFNS--CREIGGEWER 499
S+S WWR ++++ +W S C + WER
Sbjct: 406 NCSSSLWWRYLSSIDTGMGYMLQWAGSLVC*KKYWRWER 522
>TC85251 homologue to PIR|JQ2128|JQ2128 metallothionein - soybean, partial
(93%)
Length = 1554
Score = 33.1 bits (74), Expect = 0.40
Identities = 22/63 (34%), Positives = 28/63 (43%)
Frame = +1
Query: 525 GGQMGVCVRYVSVGVG*EW*GVEVEEEVVCMGGGEGGGTLFITSKCNFAG*KGGHMALKF 584
G G V + G G W GV + GGG GGG + + + F G* GG L+
Sbjct: 514 GAGAGTGVAFTGAGAGGVWTGVGD----IFGGGGNGGGNVGVETGELFGG*GGGTFGLEA 681
Query: 585 GKI 587
G I
Sbjct: 682 GAI 690
>BG647376 similar to GP|19807725|gb| CAMP-REGULATED GUANINE NUCLEOTIDE
EXCHANGE FACTOR II {Dictyostelium discoideum}, partial
(2%)
Length = 789
Score = 32.7 bits (73), Expect = 0.52
Identities = 24/87 (27%), Positives = 46/87 (52%), Gaps = 6/87 (6%)
Frame = +1
Query: 20 LMVLF---LASLKIFGIFCVIMLCVLLV---NFIGMGGLPKESIALSLLLFLRLRARRLN 73
L++LF + +L + C I+L +L++ F + P + + LLLFL L ++ ++
Sbjct: 397 LLILFAL*MLTLNSY*EVCTIILSLLMILLEGFFNLINKPSHVLIVMLLLFLILVSKLMS 576
Query: 74 DFCLISLVESMYKILAKVLANRLRYVI 100
++CLI +E +IL L Y++
Sbjct: 577 NYCLI--MEPHTQILLHCCVVLLGYIV 651
>TC81596 similar to GP|21039012|dbj|BAB92985. protein kinase {Arabidopsis
thaliana}, partial (33%)
Length = 692
Score = 32.3 bits (72), Expect = 0.68
Identities = 19/56 (33%), Positives = 31/56 (54%), Gaps = 6/56 (10%)
Frame = +2
Query: 15 INIRALMVLFLASLKIFGIFCVIMLCV------LLVNFIGMGGLPKESIALSLLLF 64
+ IR +++ LKI+ +F + +C +L+NFI G +PK I +SLL F
Sbjct: 281 LGIRLRCLIYCEILKIWSVFILKFICSRL*STRILLNFIIHGLIPKMRILISLLKF 448
>AJ497561 weakly similar to GP|10140689|gb putative non-LTR retroelement
reverse transcriptase {Oryza sativa (japonica
cultivar-group)}, partial (2%)
Length = 621
Score = 32.0 bits (71), Expect = 0.88
Identities = 17/44 (38%), Positives = 29/44 (65%)
Frame = +3
Query: 408 DWNFVCLSKEVGGYGVRRIREFNSALLGKWCWQLLIEKESLWYR 451
++N + K VGG G++ +EFN ++LGK W+LL + L+Y+
Sbjct: 300 EFNNLARHKSVGGLGLQN-QEFNISMLGKQRWKLL--SQPLYYQ 422
>BQ148771
Length = 680
Score = 31.2 bits (69), Expect = 1.5
Identities = 24/96 (25%), Positives = 39/96 (40%), Gaps = 3/96 (3%)
Frame = -3
Query: 407 VDWNFVCLSKEVGGYGVRRIREFNSALLGKWCWQLLIEKESLWYRVLSARY--NEEGGRL 464
V W + K + G G+RR+ N A + K W + SL V+ +Y +E +
Sbjct: 384 VGWETMSKPKTIYGLGLRRLDVMNKACIMKLGWSIYSGSNSLCTEVMRGKYQRSESLEEI 205
Query: 465 LDGGRSTSAWWRVIATLQREEWFNSCREIGG-EWER 499
+ S+ W+ + L E N G WE+
Sbjct: 204 FLEKPTDSSLWKALVKLWPEIERNLVDSNGNWNWEK 97
>BI308850 homologue to GP|13591616|db UDP-D-glucuronate carboxy-lyase {Pisum
sativum}, partial (60%)
Length = 629
Score = 30.4 bits (67), Expect = 2.6
Identities = 18/59 (30%), Positives = 29/59 (48%), Gaps = 4/59 (6%)
Frame = -3
Query: 384 LLNLF*NVFFFCEGSDNHKKVTWV---DWNFVCLSKEVGGYGVRRIREF-NSALLGKWC 438
+L+++ N F C H++ TWV DW V S E + ++R+ +A L WC
Sbjct: 627 ILHIWINKLFHCLSKFRHREFTWVPKIDWTSV*SSHET-DKAINQVRDIAETASLRSWC 454
>TC77141 similar to GP|14164407|dbj|BAB55806. cytosolic aldehyde
dehydrogenase {Oryza sativa (japonica cultivar-group)},
partial (93%)
Length = 1699
Score = 30.0 bits (66), Expect = 3.4
Identities = 18/32 (56%), Positives = 22/32 (68%), Gaps = 2/32 (6%)
Frame = +1
Query: 348 LNIYLLVVIWFC*NLSCHLSL--FMLFLSSRL 377
L ++LLVV+WF L+ HLSL FML L RL
Sbjct: 619 LLLWLLVVLWFLSLLNKHLSLLCFMLILLRRL 714
>TC79091 similar to GP|9294651|dbj|BAB03000.1 nucleotide sugar
epimerase-like protein {Arabidopsis thaliana}, partial
(36%)
Length = 768
Score = 30.0 bits (66), Expect = 3.4
Identities = 16/28 (57%), Positives = 19/28 (67%)
Frame = -2
Query: 524 GGGQMGVCVRYVSVGVG*EW*GVEVEEE 551
GG ++GVCV V GVG EW +E EEE
Sbjct: 386 GGEEVGVCVEVVVGGVG-EWWIIEGEEE 306
>TC77606 similar to SP|O65729|RL18_CICAR 60S ribosomal protein L18
(Fragment). [Chickpea Garbanzo] {Cicer arietinum},
partial (98%)
Length = 834
Score = 28.5 bits (62), Expect = 9.8
Identities = 14/37 (37%), Positives = 23/37 (61%)
Frame = +1
Query: 8 QCGTVIIINIRALMVLFLASLKIFGIFCVIMLCVLLV 44
QC + +I I ++ +LFLA L FCV ++ VL++
Sbjct: 679 QCCYLCLIMIFSMEILFLAPLFSVXXFCVALVTVLML 789
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.350 0.157 0.564
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,265,312
Number of Sequences: 36976
Number of extensions: 503159
Number of successful extensions: 4811
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 1987
Number of HSP's successfully gapped in prelim test: 268
Number of HSP's that attempted gapping in prelim test: 2729
Number of HSP's gapped (non-prelim): 2452
length of query: 739
length of database: 9,014,727
effective HSP length: 103
effective length of query: 636
effective length of database: 5,206,199
effective search space: 3311142564
effective search space used: 3311142564
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.9 bits)
S2: 62 (28.5 bits)
Medicago: description of AC124963.21