BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124963.19 - phase: 0 /pseudo
(206 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW691925 261 1e-70
TC84310 similar to GP|18844836|dbj|BAB85306. hypothetical protei... 77 5e-15
TC79142 52 2e-07
AW696656 31 0.38
TC88725 similar to GP|4704613|gb|AAD28178.1| monodehydroascorbat... 30 0.85
TC92539 similar to GP|16648877|gb|AAL24290.1 Unknown protein {Ar... 29 1.4
TC84479 ENBP1 28 1.9
TC87424 similar to GP|19699309|gb|AAL91265.1 AT4g10840/F25I24_50... 28 1.9
TC86932 similar to GP|20466530|gb|AAM20582.1 protein kinase-like... 28 2.5
TC79002 similar to GP|15375305|gb|AAK54745.2 putative transcript... 28 2.5
TC76510 homologue to GP|1616609|emb|CAA69931.1 PR10-1 protein {M... 28 2.5
TC79561 similar to GP|14194107|gb|AAK56248.1 At1g77260/T14N5_19 ... 28 3.2
TC83406 weakly similar to GP|19699359|gb|AAL91289.1 At1g79720/F1... 27 4.2
AW256777 weakly similar to PIR|T01269|T01 serine/threonine-speci... 27 5.5
TC86598 similar to GP|1129145|emb|CAA53078.1 acetyl-CoA C-acyltr... 27 5.5
TC90455 similar to GP|9758581|dbj|BAB09194.1 contains similarity... 27 5.5
TC88046 similar to GP|7839365|gb|AAF70243.1| S-adenosyl-L-methio... 27 5.5
TC90440 similar to GP|19310639|gb|AAL85050.1 unknown protein {Ar... 27 7.2
TC87373 homologue to PIR|A43350|A43350 formate--tetrahydrofolate... 27 7.2
BQ164993 GP|1616609|em PR10-1 protein {Medicago truncatula}, com... 26 9.4
>AW691925
Length = 649
Score = 261 bits (667), Expect = 1e-70
Identities = 124/124 (100%), Positives = 124/124 (100%)
Frame = +1
Query: 83 NLFHNLLYNWRHNLEEVFICNCPNITHGIIFNSFSFPDAPPRVKSLVLSDTGLSQPNIPQ 142
NLFHNLLYNWRHNLEEVFICNCPNITHGIIFNSFSFPDAPPRVKSLVLSDTGLSQPNIPQ
Sbjct: 1 NLFHNLLYNWRHNLEEVFICNCPNITHGIIFNSFSFPDAPPRVKSLVLSDTGLSQPNIPQ 180
Query: 143 PYFLNSLTTIKLFSCNISNQFLSSIKVLPLHTLLLGIHLSWFVRLLISAASNPPFGPFRC 202
PYFLNSLTTIKLFSCNISNQFLSSIKVLPLHTLLLGIHLSWFVRLLISAASNPPFGPFRC
Sbjct: 181 PYFLNSLTTIKLFSCNISNQFLSSIKVLPLHTLLLGIHLSWFVRLLISAASNPPFGPFRC 360
Query: 203 SLVY 206
SLVY
Sbjct: 361 SLVY 372
>TC84310 similar to GP|18844836|dbj|BAB85306. hypothetical protein {Oryza
sativa (japonica cultivar-group)}, partial (2%)
Length = 799
Score = 77.0 bits (188), Expect = 5e-15
Identities = 40/107 (37%), Positives = 65/107 (60%)
Frame = +3
Query: 2 AFGNSIPFLTSLVCSNVGSMSRQDISAISRSFQMLEVLDLGHPNLFVDGAHPNTAKALIN 61
AF +I LTSL+CSN+ S++ DI I+ +F +LE LDL +P+ ++ H + L
Sbjct: 471 AFSQNITTLTSLICSNLISLNSTDIHLIADTFPLLEELDLAYPSKIINHTHATFSTGLEA 650
Query: 62 LSLSTPRMREINLSSHSYISDNLFHNLLYNWRHNLEEVFICNCPNIT 108
LSL+ ++R++NLS H Y++ L +L N + L+EV + C +T
Sbjct: 651 LSLALIKLRKVNLSYHGYLNGTLLSHLFKNCKF-LQEVILLRCEQLT 788
>TC79142
Length = 1164
Score = 52.0 bits (123), Expect = 2e-07
Identities = 54/194 (27%), Positives = 89/194 (45%), Gaps = 19/194 (9%)
Frame = +3
Query: 3 FGNSIPFLTSLVCSNVGSMSRQDISAISRSFQMLEVLDLGHPNLF--VDGAHPNTAKALI 60
F +I LTSL CS++ + D+ I+ F +LE L+L P+ + + + N +
Sbjct: 525 FSQNIRTLTSLTCSDIDDFNTTDLFLIAECFPLLEELNLIFPSDWKSIYKNYNNYCDGIE 704
Query: 61 NLSLSTPRMREINLSSHSYISDNLFHNLLYNWRHNLEEVFICNCPNITHGIIFNSFSFPD 120
LSL+ ++R++ L +LF+ L+N LE+V + C IT I ++
Sbjct: 705 ALSLALVKLRKVKLCRFPMSDKSLFY--LFNSCKLLEDVTLFECDQITIAGIASAIR--- 869
Query: 121 APPRVKSLVLSDTGLSQPNIPQP---------------YFLNSLTTIKLFSCNISNQFLS 165
P +KSL + S+ +I L LT++ L S +IS++ L
Sbjct: 870 ERPTLKSLSFCNRTYSENSISNDDISHFKVTSDFIDSLVSLKGLTSLSLRSLDISDEMLY 1049
Query: 166 SI--KVLPLHTLLL 177
SI + LPL L L
Sbjct: 1050SIAREGLPLTRLAL 1091
>AW696656
Length = 668
Score = 30.8 bits (68), Expect = 0.38
Identities = 18/47 (38%), Positives = 24/47 (50%), Gaps = 13/47 (27%)
Frame = -3
Query: 82 DNLFHNLLYNW------------RHNLEEVFICN-CPNITHGIIFNS 115
DN FHNLLYN+ R + +F+C C N GIIF++
Sbjct: 360 DNFFHNLLYNFLFHRLCTSCGLTRGSFPWLFLCTLCFNCPRGIIFSN 220
>TC88725 similar to GP|4704613|gb|AAD28178.1| monodehydroascorbate reductase
{Brassica juncea}, partial (41%)
Length = 804
Score = 29.6 bits (65), Expect = 0.85
Identities = 12/33 (36%), Positives = 21/33 (63%)
Frame = -1
Query: 77 HSYISDNLFHNLLYNWRHNLEEVFICNCPNITH 109
HSYI+ + ++NL ++ + + ICN PNI +
Sbjct: 735 HSYITTSNYYNLFCFFQR*NQRISICNIPNIVN 637
>TC92539 similar to GP|16648877|gb|AAL24290.1 Unknown protein {Arabidopsis
thaliana}, partial (41%)
Length = 689
Score = 28.9 bits (63), Expect = 1.4
Identities = 17/66 (25%), Positives = 31/66 (46%)
Frame = +2
Query: 122 PPRVKSLVLSDTGLSQPNIPQPYFLNSLTTIKLFSCNISNQFLSSIKVLPLHTLLLGIHL 181
P ++ S + + ++ NI P+ + + L SC+ S + + + +LG L
Sbjct: 17 PTQIHSTFIKNQYSTKTNIITPFHIKIQQNLHLQSCSTSLRHFIEPVTVQMKRGVLGNGL 196
Query: 182 SWFVRL 187
SW VRL
Sbjct: 197 SWVVRL 214
>TC84479 ENBP1
Length = 5108
Score = 28.5 bits (62), Expect = 1.9
Identities = 18/49 (36%), Positives = 21/49 (42%), Gaps = 10/49 (20%)
Frame = -3
Query: 65 STPRMREINLSSHSYISDNLFHNLLYN----------WRHNLEEVFICN 103
ST +R NL+ S NL+HNLL W HNL I N
Sbjct: 1725 STTLLRPCNLTGTSNFMINLYHNLLSTF*TFWSTTLLWPHNLTSNIITN 1579
>TC87424 similar to GP|19699309|gb|AAL91265.1 AT4g10840/F25I24_50
{Arabidopsis thaliana}, partial (32%)
Length = 1014
Score = 28.5 bits (62), Expect = 1.9
Identities = 18/50 (36%), Positives = 22/50 (44%)
Frame = +2
Query: 147 NSLTTIKLFSCNISNQFLSSIKVLPLHTLLLGIHLSWFVRLLISAASNPP 196
NS+TT I QFLS + PLHT L L F + + PP
Sbjct: 56 NSITTFPFVLVEIVPQFLSLFYLSPLHTNFLHTILIQFTMPGLVSPGGPP 205
>TC86932 similar to GP|20466530|gb|AAM20582.1 protein kinase-like protein
{Arabidopsis thaliana}, partial (73%)
Length = 1464
Score = 28.1 bits (61), Expect = 2.5
Identities = 12/40 (30%), Positives = 23/40 (57%), Gaps = 5/40 (12%)
Frame = +2
Query: 78 SYISDNLFHNLLYNWRHNLEEVFICNC-----PNITHGII 112
S+ + N+F+ ++ WR LE +F +C ++T GI+
Sbjct: 1322 SFFTANIFYQIVVFWRSRLELLFYVHCCKYHAAHLTRGIV 1441
>TC79002 similar to GP|15375305|gb|AAK54745.2 putative transcription factor
MYB124 {Arabidopsis thaliana}, partial (54%)
Length = 2304
Score = 28.1 bits (61), Expect = 2.5
Identities = 14/51 (27%), Positives = 27/51 (52%), Gaps = 5/51 (9%)
Frame = -3
Query: 152 IKLFSCNISNQFLSSIKVLPLHTLLLGIHLSWFV-----RLLISAASNPPF 197
+ LF N+ +F +++ VLP T + +HL + + + S+ +PPF
Sbjct: 901 LALFLHNVEKRFFTALSVLPETTFAISVHLFPNIF*ASHKSMSSSGDHPPF 749
>TC76510 homologue to GP|1616609|emb|CAA69931.1 PR10-1 protein {Medicago
truncatula}, partial (97%)
Length = 658
Score = 28.1 bits (61), Expect = 2.5
Identities = 21/77 (27%), Positives = 37/77 (47%)
Frame = -3
Query: 106 NITHGIIFNSFSFPDAPPRVKSLVLSDTGLSQPNIPQPYFLNSLTTIKLFSCNISNQFLS 165
N+ IF+ S PP + L T S+ ++ + YF++ +T F + F S
Sbjct: 404 NLASKEIFSPVSGSPPPPTML*L*AKLTSSSKSSLCESYFVSPPSTKVSFFMVSAPPFPS 225
Query: 166 SIKVLPLHTLLLGIHLS 182
+I +L + ++ GI LS
Sbjct: 224 TISILWMASITFGIRLS 174
>TC79561 similar to GP|14194107|gb|AAK56248.1 At1g77260/T14N5_19
{Arabidopsis thaliana}, partial (33%)
Length = 1092
Score = 27.7 bits (60), Expect = 3.2
Identities = 16/34 (47%), Positives = 21/34 (61%)
Frame = +1
Query: 160 SNQFLSSIKVLPLHTLLLGIHLSWFVRLLISAAS 193
S+ LS+ LPLH+LLL H F LL+S+ S
Sbjct: 37 SHNLLSNPSHLPLHSLLLFNHHYLFPHLLLSSFS 138
>TC83406 weakly similar to GP|19699359|gb|AAL91289.1 At1g79720/F19K16_30
{Arabidopsis thaliana}, partial (51%)
Length = 1197
Score = 27.3 bits (59), Expect = 4.2
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = -1
Query: 132 DTGLSQPNIPQPYFLNSLTTIKLFSCNISNQFLSSIK 168
D+G QP P +FL ++ T+ +F C+ S + IK
Sbjct: 183 DSGRMQPFEPNWWFLCNICTLNIFFCSSSQPPFAMIK 73
>AW256777 weakly similar to PIR|T01269|T01 serine/threonine-specific protein
kinase (EC 2.7.1.-) F27F23.1 - Arabidopsis thaliana,
partial (13%)
Length = 373
Score = 26.9 bits (58), Expect = 5.5
Identities = 17/43 (39%), Positives = 25/43 (57%)
Frame = +3
Query: 4 GNSIPFLTSLVCSNVGSMSRQDISAISRSFQMLEVLDLGHPNL 46
GN+IP +TSL S+ G +IS+ ML+ LDL + +L
Sbjct: 231 GNNIPRITSLNLSSSGLTG--EISSSISKLTMLQYLDLSNNSL 353
>TC86598 similar to GP|1129145|emb|CAA53078.1 acetyl-CoA C-acyltransferase
{Mangifera indica}, partial (89%)
Length = 1534
Score = 26.9 bits (58), Expect = 5.5
Identities = 11/29 (37%), Positives = 16/29 (54%)
Frame = +3
Query: 90 YNWRHNLEEVFICNCPNITHGIIFNSFSF 118
Y+W NL+E F C C + HG++ F
Sbjct: 975 YSW--NLQEFFCCWCRSCCHGVLVQLLLF 1055
>TC90455 similar to GP|9758581|dbj|BAB09194.1 contains similarity to unknown
protein~dbj|BAA76827.1~gene_id:MBD2.12 {Arabidopsis
thaliana}, partial (16%)
Length = 636
Score = 26.9 bits (58), Expect = 5.5
Identities = 18/51 (35%), Positives = 25/51 (48%), Gaps = 5/51 (9%)
Frame = +3
Query: 86 HNLLYNWRHNLEEVFICN-----CPNITHGIIFNSFSFPDAPPRVKSLVLS 131
HNL+Y H L+ + C P+I ++ F DAP +K LVLS
Sbjct: 372 HNLVYEKSHYLKAIKACKDFKSKYPDIE--LVPEEEFFRDAPKDIKDLVLS 518
>TC88046 similar to GP|7839365|gb|AAF70243.1| S-adenosyl-L-methionine
Mg-protoporphyrin IX methyltranserase {Nicotiana
tabacum}, partial (84%)
Length = 1213
Score = 26.9 bits (58), Expect = 5.5
Identities = 18/64 (28%), Positives = 36/64 (56%)
Frame = -3
Query: 129 VLSDTGLSQPNIPQPYFLNSLTTIKLFSCNISNQFLSSIKVLPLHTLLLGIHLSWFVRLL 188
+L+ G+SQPNIP LNS+ I + + + +++LP + ++ ++F+RL
Sbjct: 428 ILNSLGMSQPNIP----LNSINIISITVNLLPSLKPVVVEILPHNFFVI---TAYFLRLF 270
Query: 189 ISAA 192
S++
Sbjct: 269 PSSS 258
>TC90440 similar to GP|19310639|gb|AAL85050.1 unknown protein {Arabidopsis
thaliana}, partial (80%)
Length = 1429
Score = 26.6 bits (57), Expect = 7.2
Identities = 12/33 (36%), Positives = 19/33 (57%), Gaps = 3/33 (9%)
Frame = +1
Query: 91 NWRHNLEEVFICNC---PNITHGIIFNSFSFPD 120
+W+ IC+C PNI IIF++ +FP+
Sbjct: 670 HWKDIYSRCIICSCNRLPNIWXKIIFHAEAFPN 768
>TC87373 homologue to PIR|A43350|A43350 formate--tetrahydrofolate ligase (EC
6.3.4.3) - spinach, partial (67%)
Length = 1591
Score = 26.6 bits (57), Expect = 7.2
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +1
Query: 154 LFSCNISNQFLSSIKVLPLHTLLLGIHLSWFVRL 187
L C + + L + L TLL+GI L W +RL
Sbjct: 352 LLLCRLWKELLFLSMQVHLQTLLMGILLLWLIRL 453
>BQ164993 GP|1616609|em PR10-1 protein {Medicago truncatula}, complete
Length = 603
Score = 26.2 bits (56), Expect = 9.4
Identities = 20/77 (25%), Positives = 37/77 (47%)
Frame = +2
Query: 106 NITHGIIFNSFSFPDAPPRVKSLVLSDTGLSQPNIPQPYFLNSLTTIKLFSCNISNQFLS 165
N+ IF++ S PP + L T ++ + + YF++ +T F + F S
Sbjct: 284 NLDSKEIFSTVSGSPPPPTML*L*AKLTSSTKSTLCKSYFVSPPSTKVSFLMVPAPPFPS 463
Query: 166 SIKVLPLHTLLLGIHLS 182
+I +L + ++ GI LS
Sbjct: 464 TISILWMASITFGIRLS 514
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.324 0.139 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,478,755
Number of Sequences: 36976
Number of extensions: 149684
Number of successful extensions: 1319
Number of sequences better than 10.0: 72
Number of HSP's better than 10.0 without gapping: 1312
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1319
length of query: 206
length of database: 9,014,727
effective HSP length: 92
effective length of query: 114
effective length of database: 5,612,935
effective search space: 639874590
effective search space used: 639874590
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 56 (26.2 bits)
Medicago: description of AC124963.19