BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124963.14 + phase: 0
(294 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86353 similar to GP|15450733|gb|AAK96638.1 At2g36220/F2H17.17 ... 40 0.001
TC78696 similar to GP|15450733|gb|AAK96638.1 At2g36220/F2H17.17 ... 37 0.007
AW559955 similar to GP|21593487|gb| unknown {Arabidopsis thalian... 35 0.034
TC77517 similar to PIR|T51159|T51159 HMG protein [imported] - Ar... 35 0.034
TC89113 homologue to GP|19911575|dbj|BAB86891. syringolide-induc... 34 0.059
BQ140761 weakly similar to GP|18076245|emb phosphophoryn {Rattus... 33 0.10
TC79366 similar to GP|21536751|gb|AAM61083.1 unknown {Arabidopsi... 33 0.13
TC77445 homologue to GP|19911575|dbj|BAB86891. syringolide-induc... 33 0.17
BG453609 similar to GP|603269|gb|AA Yer036cp {Saccharomyces cere... 32 0.22
BQ138520 similar to SP|O59953|RL5_ 60S ribosomal protein L5 (CPR... 32 0.38
TC90910 homologue to GP|21689883|gb|AAM67502.1 unknown protein {... 31 0.50
CB065368 weakly similar to GP|14025115|dbj sarcosine oxidase bet... 30 0.85
BG644710 similar to GP|3746570|gb|A putative pyruvate oxidoreduc... 30 0.85
TC77629 similar to GP|6648962|gb|AAF21309.1| seed maturation pro... 30 1.1
AW686016 weakly similar to GP|3581899|emb hypothetical serine-ri... 30 1.1
TC76514 homologue to PIR|T09648|T09648 nucleolin homolog nuM1 - ... 30 1.1
BQ750959 similar to GP|18376329|emb related to cell surface ferr... 29 1.9
TC86965 similar to GP|22022558|gb|AAM83236.1 At5g12410 {Arabidop... 29 1.9
TC80913 similar to PIR|T05616|T05616 hypothetical protein F20D10... 29 2.5
TC76523 homologue to PIR|T09648|T09648 nucleolin homolog nuM1 - ... 29 2.5
>TC86353 similar to GP|15450733|gb|AAK96638.1 At2g36220/F2H17.17
{Arabidopsis thaliana}, partial (12%)
Length = 1351
Score = 40.0 bits (92), Expect = 0.001
Identities = 30/113 (26%), Positives = 55/113 (48%)
Frame = +1
Query: 43 MFVLRNKKTIETTQEPSSPKVTCMGQVRAKRSSSKSSTTPTHHWWIKKPNPCRCRLVWPK 102
+F R+ ++ EPSSPKV+CMG+VR+KR K+ST K R K
Sbjct: 463 LFPKRSASVGKSFVEPSSPKVSCMGKVRSKR--GKTSTVTAAAKAAVKEKTARTERKQSK 636
Query: 103 WAFFRRKKPTKPKQDSVKSESNRNSNFTLEDEQRVSVSVESVDNAIVSNSNCS 155
+FF+ + + +++ +R ++ + D ++ + SV ++ +S S
Sbjct: 637 HSFFKSFRAI--FRSGGRNKHDRMNDSSALDSSSITKNTNSVSDSKARDSTAS 789
>TC78696 similar to GP|15450733|gb|AAK96638.1 At2g36220/F2H17.17
{Arabidopsis thaliana}, partial (13%)
Length = 1108
Score = 37.4 bits (85), Expect = 0.007
Identities = 23/60 (38%), Positives = 34/60 (56%), Gaps = 8/60 (13%)
Frame = +3
Query: 30 AGSRSSRRSRSSP----MFVLRNK----KTIETTQEPSSPKVTCMGQVRAKRSSSKSSTT 81
A +R RR+ P +F R+ K+ T EPSSPKV+C+G+VR+KR ++ T
Sbjct: 612 AEARGCRRNHHKPGNTRLFPKRSASIGGKSDSTVFEPSSPKVSCIGRVRSKRGHNRRLRT 791
>AW559955 similar to GP|21593487|gb| unknown {Arabidopsis thaliana}, partial
(7%)
Length = 411
Score = 35.0 bits (79), Expect = 0.034
Identities = 20/67 (29%), Positives = 38/67 (55%), Gaps = 1/67 (1%)
Frame = +1
Query: 56 QEPSSPKVTCMGQVRAKRSSSKSSTTPTHHWWIKKPNPCRCRLVW-PKWAFFRRKKPTKP 114
+EP+SPKV+CMG++++K+ K H I+ P + L+W K ++ ++ K
Sbjct: 121 KEPTSPKVSCMGEIKSKKKKKKKE--KKLHKGIQNVVPNKNVLLWISKGSYEGGRQGGKE 294
Query: 115 KQDSVKS 121
K+ ++ S
Sbjct: 295 KESAIVS 315
>TC77517 similar to PIR|T51159|T51159 HMG protein [imported] - Arabidopsis
thaliana, partial (70%)
Length = 907
Score = 35.0 bits (79), Expect = 0.034
Identities = 28/116 (24%), Positives = 53/116 (45%)
Frame = +3
Query: 107 RRKKPTKPKQDSVKSESNRNSNFTLEDEQRVSVSVESVDNAIVSNSNCSTPPKNALLLTR 166
+ KK K K+D K + ++ F ++ R + E+ + VS + K L T+
Sbjct: 231 KTKKEKKAKKDPNKPKRPPSAFFVFLEDFRKTFKAENPNVKAVSAVGKAGGEKWKSL-TK 407
Query: 167 CRSAPYRSSSLASRFWSSPLRNEEETESTSIDDNEESSSQSQKRESVSDKEDSIGE 222
APY + + + L N + +S DD+EE S + V++++++ GE
Sbjct: 408 AEKAPYEAKAAKRKVEYEKLMNAYNNKPSSADDDEEESDKD--NSEVNNEDEASGE 569
>TC89113 homologue to GP|19911575|dbj|BAB86891. syringolide-induced protein
14-1-1 {Glycine max}, partial (24%)
Length = 703
Score = 34.3 bits (77), Expect = 0.059
Identities = 12/24 (50%), Positives = 19/24 (79%)
Frame = +2
Query: 56 QEPSSPKVTCMGQVRAKRSSSKSS 79
+EP+SPK++CMGQ++ K+ K S
Sbjct: 374 EEPTSPKISCMGQIKHKKKQIKKS 445
>BQ140761 weakly similar to GP|18076245|emb phosphophoryn {Rattus
norvegicus}, partial (9%)
Length = 620
Score = 33.5 bits (75), Expect = 0.10
Identities = 30/122 (24%), Positives = 53/122 (42%), Gaps = 3/122 (2%)
Frame = +1
Query: 117 DSVKSESNRNSNFTLEDEQRVSVSVESVDNAIVSNSNCSTP---PKNALLLTRCRSAPYR 173
DS S+S+ +S+ + D S +S D++ +S+ P PK ++
Sbjct: 250 DSSDSDSSDSSDGSDSDSDASDDSDDSSDSSSDDSSSDEEPVPAPKKEKAKKAKKAKSVS 429
Query: 174 SSSLASRFWSSPLRNEEETESTSIDDNEESSSQSQKRESVSDKEDSIGERIGLTKDLENV 233
SSS +S SS +E E + + ++++SSS S +S SD D ++
Sbjct: 430 SSSDSSDDSSSESESEPEPXNVPLPESDDSSSASSDSDSSSDSSSDSSSDSSSXSDXDSS 609
Query: 234 QE 235
E
Sbjct: 610 SE 615
>TC79366 similar to GP|21536751|gb|AAM61083.1 unknown {Arabidopsis thaliana},
partial (79%)
Length = 1688
Score = 33.1 bits (74), Expect = 0.13
Identities = 26/81 (32%), Positives = 40/81 (49%)
Frame = -1
Query: 114 PKQDSVKSESNRNSNFTLEDEQRVSVSVESVDNAIVSNSNCSTPPKNALLLTRCRSAPYR 173
P + SNRNS + D + +SVES+ S+S +TPP +A+ C +
Sbjct: 1022 PSSSTTG*PSNRNSTGKIYDSSSLILSVESISRYGASSSAATTPPLDAMY---CPINFHL 852
Query: 174 SSSLASRFWSSPLRNEEETES 194
SSS+ SP +E+T+S
Sbjct: 851 SSSIP*LGSPSP-NTKEKTDS 792
>TC77445 homologue to GP|19911575|dbj|BAB86891. syringolide-induced protein
14-1-1 {Glycine max}, partial (22%)
Length = 978
Score = 32.7 bits (73), Expect = 0.17
Identities = 11/16 (68%), Positives = 16/16 (99%)
Frame = +2
Query: 55 TQEPSSPKVTCMGQVR 70
TQEP+SPK++CMGQ++
Sbjct: 323 TQEPTSPKISCMGQIK 370
>BG453609 similar to GP|603269|gb|AA Yer036cp {Saccharomyces cerevisiae},
partial (14%)
Length = 656
Score = 32.3 bits (72), Expect = 0.22
Identities = 41/127 (32%), Positives = 49/127 (38%), Gaps = 8/127 (6%)
Frame = +3
Query: 12 PARTDKFPP-PLMRFLRTNAGSRSSRRSRSSPMFVLRNKKTIETTQEP--SSPKVTCMGQ 68
P T + PP P +R S +RRSRSSP R T+ T P SP+ +
Sbjct: 291 PRPTPRMPPRPTLRTATRPRTSS*TRRSRSSP---CRRTSTVCLTVSPPVCSPRSSSRAM 461
Query: 69 VRAK--RSSS---KSSTTPTHHWWIKKPNPCRCRLVWPKWAFFRRKKPTKPKQDSVKSES 123
R+ RSSS SSTTP C R V + F R S S S
Sbjct: 462 SRSSLHRSSSTEKSSSTTPPSRSTTADDTVCWVRTVAERPLFSR-------LSTSASSPS 620
Query: 124 NRNSNFT 130
S FT
Sbjct: 621 QSTSTFT 641
>BQ138520 similar to SP|O59953|RL5_ 60S ribosomal protein L5 (CPR4).
{Neurospora crassa}, partial (63%)
Length = 584
Score = 31.6 bits (70), Expect = 0.38
Identities = 29/98 (29%), Positives = 41/98 (41%)
Frame = +2
Query: 1 MKLSTTKSISSPARTDKFPPPLMRFLRTNAGSRSSRRSRSSPMFVLRNKKTIETTQEPSS 60
M+ ST S +SP RT +R + T + + S SSPM T+ T P
Sbjct: 110 MRPSTASSSASPTRTSSLRLSPLRLVATRSSWLRTLTS-SSPMA----SPTV*PTGLPPM 274
Query: 61 PKVTCMGQVRAKRSSSKSSTTPTHHWWIKKPNPCRCRL 98
P V C V ++ SSS + + P+P RL
Sbjct: 275 PLVFCSPAVSSRSSSSTRLSPVLRRPMVSTPSPRPPRL 388
>TC90910 homologue to GP|21689883|gb|AAM67502.1 unknown protein {Arabidopsis
thaliana}, partial (32%)
Length = 683
Score = 31.2 bits (69), Expect = 0.50
Identities = 18/63 (28%), Positives = 30/63 (47%), Gaps = 2/63 (3%)
Frame = +1
Query: 38 SRSSPMFVLRNKKTIETTQEPSSPKVTCMGQVRAKRSSSK--SSTTPTHHWWIKKPNPCR 95
S+S P +L+ K T+ PSS + +S+ K +TT H +W+ + P
Sbjct: 484 SKSCPKILLKGSKEWNTSSCPSSSSKAQSYSSLSTKSNKKCFGATTSIHCFWLFRKYPST 663
Query: 96 CRL 98
CR+
Sbjct: 664 CRV 672
>CB065368 weakly similar to GP|14025115|dbj sarcosine oxidase beta subunit
{Mesorhizobium loti}, partial (15%)
Length = 592
Score = 30.4 bits (67), Expect = 0.85
Identities = 27/104 (25%), Positives = 42/104 (39%), Gaps = 4/104 (3%)
Frame = -1
Query: 86 WWIKKPNPCRCRLVWPKWAFFRRKKPTKPKQDSVKSESNRNSNFTLEDEQRVSVS----V 141
W + P P C WPK A RR PT+ ++ S S + ++++ S
Sbjct: 577 WCRQWPAPVHC*--WPKPASARRSTPTRCRRWSPSHSSRFSIHWSVPRRCTAMCSRPAAA 404
Query: 142 ESVDNAIVSNSNCSTPPKNALLLTRCRSAPYRSSSLASRFWSSP 185
S A+ + + C+TP + C P S SR W +P
Sbjct: 403 RSSSVAVPTPTRCTTPARPWTSRKVCWPTP----SKCSRSWPTP 284
>BG644710 similar to GP|3746570|gb|A putative pyruvate oxidoreductase; POR
{Brachyspira pilosicoli}, partial (5%)
Length = 661
Score = 30.4 bits (67), Expect = 0.85
Identities = 15/48 (31%), Positives = 28/48 (58%), Gaps = 6/48 (12%)
Frame = +3
Query: 34 SSRRSRSSPMFVLRNKKT------IETTQEPSSPKVTCMGQVRAKRSS 75
+ + +R +P+F+L KKT I T++ ++ K+ +G RA+R S
Sbjct: 87 AQKLTRPNPLFLLHQKKTQIPLLFIPVTEQTNTTKIRRVGDTRARRRS 230
>TC77629 similar to GP|6648962|gb|AAF21309.1| seed maturation protein PM23
{Glycine max}, partial (76%)
Length = 1741
Score = 30.0 bits (66), Expect = 1.1
Identities = 27/95 (28%), Positives = 43/95 (44%), Gaps = 2/95 (2%)
Frame = +3
Query: 74 SSSKSSTTPTHHWWIKKPNPCRCRLVWPKWAFFR-RKKPTK-PKQDSVKSESNRNSNFTL 131
SSS SS+ P H + +P+P ++F R PTK P V + S+ +SN
Sbjct: 120 SSSSSSSLPLHRF---RPSP--------SFSFSPLRSNPTKSPSPFLVLASSSHDSNDFT 266
Query: 132 EDEQRVSVSVESVDNAIVSNSNCSTPPKNALLLTR 166
+ +S ++ ++ VSN PP A + R
Sbjct: 267 SKKSALSELIQEIEPLDVSNIQKDVPPTTADAMKR 371
>AW686016 weakly similar to GP|3581899|emb hypothetical serine-rich protein
{Schizosaccharomyces pombe}, partial (4%)
Length = 593
Score = 30.0 bits (66), Expect = 1.1
Identities = 25/84 (29%), Positives = 43/84 (50%)
Frame = -3
Query: 149 VSNSNCSTPPKNALLLTRCRSAPYRSSSLASRFWSSPLRNEEETESTSIDDNEESSSQSQ 208
+++S+ S P N+ LLT SAP + S S+ SS S++I + ESS+ S
Sbjct: 387 LTSSSSSKPGCNSFLLTFSASAP-KFRSTVSKT*SS---------SSTIPSSRESSTNST 238
Query: 209 KRESVSDKEDSIGERIGLTKDLEN 232
K ++S+ S L++ +E+
Sbjct: 237 KSSTLSNSHSSSPSSAILSESIES 166
>TC76514 homologue to PIR|T09648|T09648 nucleolin homolog nuM1 - alfalfa,
partial (61%)
Length = 1288
Score = 30.0 bits (66), Expect = 1.1
Identities = 27/115 (23%), Positives = 48/115 (41%), Gaps = 7/115 (6%)
Frame = +3
Query: 113 KPKQDSVKSESN----RNSNFTLEDEQRVSVSVESVDNAIVSNSNCSTPPKNALLLTRCR 168
KP +V S++ + ++ DE S E A ++ N S P K A +
Sbjct: 678 KPAAKAVPSKNGSVPAKKADTESSDEDSESSDEEDKKPAAKASKNVSAPTKKAASSSDEE 857
Query: 169 SAPYRSSSLASRFWSSPL---RNEEETESTSIDDNEESSSQSQKRESVSDKEDSI 220
S ++ S P + ++ S S D++++SSS K+ + KED +
Sbjct: 858 SDEESDEDEDAKPVSKPAAVAKKSKKDSSDSDDEDDDSSSDEDKKPVAAKKEDKM 1022
>BQ750959 similar to GP|18376329|emb related to cell surface ferroxidase
{Neurospora crassa}, partial (13%)
Length = 663
Score = 29.3 bits (64), Expect = 1.9
Identities = 19/66 (28%), Positives = 28/66 (41%), Gaps = 2/66 (3%)
Frame = +2
Query: 34 SSRRSRSSPMFVLRNKKTIETTQEPSSPKV--TCMGQVRAKRSSSKSSTTPTHHWWIKKP 91
+ RR+RS P R + PS P+ T G R + ++ + P WW + P
Sbjct: 248 ADRRARSRPGGPARRRPRAPRRCPPSGPRRRGTPPGGPRGRPAAPAARRGP-RRWWRRDP 424
Query: 92 NPCRCR 97
PC R
Sbjct: 425 GPCATR 442
>TC86965 similar to GP|22022558|gb|AAM83236.1 At5g12410 {Arabidopsis
thaliana}, partial (59%)
Length = 1455
Score = 29.3 bits (64), Expect = 1.9
Identities = 12/28 (42%), Positives = 20/28 (70%)
Frame = +1
Query: 191 ETESTSIDDNEESSSQSQKRESVSDKED 218
+++S+SIDD+EE ++Q E +KED
Sbjct: 325 DSDSSSIDDDEEEEEKAQVPEGGEEKED 408
>TC80913 similar to PIR|T05616|T05616 hypothetical protein F20D10.10 -
Arabidopsis thaliana, partial (7%)
Length = 995
Score = 28.9 bits (63), Expect = 2.5
Identities = 24/87 (27%), Positives = 37/87 (41%), Gaps = 1/87 (1%)
Frame = +2
Query: 171 PYRSSSLASRFWSSPLRNEEETESTSIDDNEES-SSQSQKRESVSDKEDSIGERIGLTKD 229
P SS+ SRF + P EEE E ++N+E Q S E L
Sbjct: 659 PLLSSASVSRFSTIPENEEEELEKEENEENKEPVEFQGLDVSSTKTFEAFFLPETALVAS 838
Query: 230 LENVQELLLKGRVKKEEEDSAVARPVV 256
++ + L+ +VK + ++ V RP V
Sbjct: 839 NKSFETLIAVLKVKAKPYNAVVNRPPV 919
>TC76523 homologue to PIR|T09648|T09648 nucleolin homolog nuM1 - alfalfa,
partial (69%)
Length = 1615
Score = 28.9 bits (63), Expect = 2.5
Identities = 17/44 (38%), Positives = 25/44 (56%), Gaps = 1/44 (2%)
Frame = -3
Query: 41 SPMFVLRNKKT-IETTQEPSSPKVTCMGQVRAKRSSSKSSTTPT 83
S +F R+K IE++ SS T + + +S+S SSTTPT
Sbjct: 1316 SSLFPFRSKAWFIESSTTTSSSSTTSTATIPSTKSTSTSSTTPT 1185
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.310 0.124 0.356
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,052,664
Number of Sequences: 36976
Number of extensions: 152579
Number of successful extensions: 972
Number of sequences better than 10.0: 59
Number of HSP's better than 10.0 without gapping: 953
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 967
length of query: 294
length of database: 9,014,727
effective HSP length: 95
effective length of query: 199
effective length of database: 5,502,007
effective search space: 1094899393
effective search space used: 1094899393
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC124963.14