BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124953.9 - phase: 0
(95 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG644643 similar to PIR|H86440|H864 unknown protein [imported] -... 71 6e-14
TC89053 similar to GP|17104693|gb|AAL34235.1 unknown protein {Ar... 30 0.12
TC87285 similar to GP|15450930|gb|AAK96736.1 Unknown protein {Ar... 29 0.35
BQ158042 homologue to GP|13430510|gb| putative translation elong... 29 0.35
BE204908 28 0.45
BQ154516 28 0.59
TC90155 similar to SP|Q9ZRF1|MTD_FRAAN Probable mannitol dehydro... 27 1.0
TC88762 similar to GP|22136928|gb|AAM91808.1 unknown protein {Ar... 27 1.0
BM778907 27 1.3
TC82695 similar to PIR|T48394|T48394 hypothetical protein F17C15... 27 1.3
TC77342 similar to GP|13785211|emb|CAC37357. putative membrane p... 27 1.3
TC88557 weakly similar to GP|17065320|gb|AAL32814.1 Unknown prot... 27 1.7
TC92525 27 1.7
TC78900 homologue to SP|P51819|HS83_PHANI Heat shock protein 83.... 26 2.2
TC81645 weakly similar to GP|22136076|gb|AAM91116.1 unknown prot... 26 2.2
AW127179 similar to GP|5679340|emb|C hypothetical protein {Galeg... 26 2.2
AW207949 26 2.2
TC84590 similar to SP|P26415|NO14_PEA Nodulin 14 precursor (N-14... 26 2.2
BE941074 26 2.9
TC88212 similar to GP|10178030|dbj|BAB11513. dbj|BAA90353.1~gene... 26 2.9
>BG644643 similar to PIR|H86440|H864 unknown protein [imported] - Arabidopsis
thaliana, partial (3%)
Length = 758
Score = 71.2 bits (173), Expect = 6e-14
Identities = 44/89 (49%), Positives = 46/89 (51%), Gaps = 12/89 (13%)
Frame = +2
Query: 1 MAEGSNTPVLLPDLLKNTPSNIARLKDVVEQSKARQKYLAHTNSSSDGGDVRWYFCKTSL 60
MAEGSNTPVLLPDLLKNTPSNIARL+DV VRWYFCKTSL
Sbjct: 512 MAEGSNTPVLLPDLLKNTPSNIARLEDV----------------------VRWYFCKTSL 625
Query: 61 AHNGMYYVL------------SSICSFCC 77
A N + L S C CC
Sbjct: 626 APNVLGKTLPYGACYHHGSTHHSCCCHCC 712
>TC89053 similar to GP|17104693|gb|AAL34235.1 unknown protein {Arabidopsis
thaliana}, partial (57%)
Length = 1327
Score = 30.4 bits (67), Expect = 0.12
Identities = 15/35 (42%), Positives = 19/35 (53%)
Frame = +1
Query: 55 FCKTSLAHNGMYYVLSSICSFCCINLILYYFTTLL 89
FC SL HN M + IC FCCI+ + T +L
Sbjct: 1075 FCSASL-HNVMLFC*IRICFFCCIHFCMSIHTGIL 1176
>TC87285 similar to GP|15450930|gb|AAK96736.1 Unknown protein {Arabidopsis
thaliana}, partial (67%)
Length = 1122
Score = 28.9 bits (63), Expect = 0.35
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = +1
Query: 72 ICSFCCINLILYYFTTLLV 90
+CSFCC+ LIL+Y L +
Sbjct: 997 VCSFCCLVLILFYTFNLYI 1053
>BQ158042 homologue to GP|13430510|gb| putative translation elongation factor
eEF-1 alpha chain A4 {Arabidopsis thaliana}, partial
(12%)
Length = 933
Score = 28.9 bits (63), Expect = 0.35
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = +2
Query: 70 SSICSFCCINLILYYFTTLLVCI 92
SSI +FCCI +ILY ++ VCI
Sbjct: 758 SSIDTFCCIFIILYSVSSYDVCI 826
>BE204908
Length = 646
Score = 28.5 bits (62), Expect = 0.45
Identities = 10/23 (43%), Positives = 16/23 (69%)
Frame = +1
Query: 68 VLSSICSFCCINLILYYFTTLLV 90
+L CS C+ L++YYF+ LL+
Sbjct: 94 LLLCFCSKICVKLLVYYFSNLLI 162
>BQ154516
Length = 506
Score = 28.1 bits (61), Expect = 0.59
Identities = 15/29 (51%), Positives = 19/29 (64%), Gaps = 2/29 (6%)
Frame = +2
Query: 54 YFCKTSLAHNGMYYVLSSICSFC--CINL 80
+F K LA M Y+ SS+CSFC C+NL
Sbjct: 248 FFLKAWLAQ--MDYISSSLCSFCLQCLNL 328
>TC90155 similar to SP|Q9ZRF1|MTD_FRAAN Probable mannitol dehydrogenase (EC
1.1.1.255) (NAD-dependent mannitol dehydrogenase).
[Strawberry], partial (78%)
Length = 1051
Score = 27.3 bits (59), Expect = 1.0
Identities = 8/29 (27%), Positives = 18/29 (61%)
Frame = -1
Query: 53 WYFCKTSLAHNGMYYVLSSICSFCCINLI 81
W+ C +++ H +++V S CC+++I
Sbjct: 856 WFQCVSNINHKSIFHVSLSKTLHCCVDII 770
>TC88762 similar to GP|22136928|gb|AAM91808.1 unknown protein {Arabidopsis
thaliana}, partial (3%)
Length = 805
Score = 27.3 bits (59), Expect = 1.0
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = +3
Query: 73 CSFCCINLILYYFTTLLVCIP 93
C F C N +L FT+++V P
Sbjct: 672 CEFSCFNFLLLQFTSIIVLFP 734
>BM778907
Length = 198
Score = 26.9 bits (58), Expect = 1.3
Identities = 8/23 (34%), Positives = 16/23 (68%)
Frame = +1
Query: 65 MYYVLSSICSFCCINLILYYFTT 87
+YY CS+C + +I++++TT
Sbjct: 4 LYYESMRFCSYCSVCVIVWFYTT 72
>TC82695 similar to PIR|T48394|T48394 hypothetical protein F17C15.90 -
Arabidopsis thaliana, partial (8%)
Length = 745
Score = 26.9 bits (58), Expect = 1.3
Identities = 12/27 (44%), Positives = 18/27 (66%), Gaps = 2/27 (7%)
Frame = +3
Query: 68 VLSSICSFCCINL--ILYYFTTLLVCI 92
VL +C F C+ L +++FTT LVC+
Sbjct: 153 VLLFVCLFVCLILHYTIFHFTTTLVCL 233
>TC77342 similar to GP|13785211|emb|CAC37357. putative membrane protein
{Solanum tuberosum}, partial (47%)
Length = 1581
Score = 26.9 bits (58), Expect = 1.3
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = +1
Query: 67 YVLSSICSFCCINLILYYFTTLLVCI 92
Y+L +C F C+ LY+F L +CI
Sbjct: 1471 YLLLGVC-FLCVRYTLYHFLFLHICI 1545
>TC88557 weakly similar to GP|17065320|gb|AAL32814.1 Unknown protein
{Arabidopsis thaliana}, partial (38%)
Length = 673
Score = 26.6 bits (57), Expect = 1.7
Identities = 21/81 (25%), Positives = 32/81 (38%), Gaps = 4/81 (4%)
Frame = -1
Query: 1 MAEGSNTPVLLPDLLKNTPSNIARLKDVVEQSKA----RQKYLAHTNSSSDGGDVRWYFC 56
M E + +++ + K TPSNI +S A R + HT S C
Sbjct: 583 MIESQHNALIMSTMKKQTPSNI--------KSSAF*CLRCCFCKHTCSLCFSFSCLGLPC 428
Query: 57 KTSLAHNGMYYVLSSICSFCC 77
+ NG + + + CS CC
Sbjct: 427 RCLSLSNGSFCLRNCFCSCCC 365
>TC92525
Length = 405
Score = 26.6 bits (57), Expect = 1.7
Identities = 15/35 (42%), Positives = 18/35 (50%), Gaps = 4/35 (11%)
Frame = +1
Query: 64 GMYYVLSSICSFCCINLILYYFTTLLVC----IPF 94
G Y + +ICS I I+YY T L C IPF
Sbjct: 247 GPRYQICNICSLSYIKYIMYY*TCL*TC*DSKIPF 351
>TC78900 homologue to SP|P51819|HS83_PHANI Heat shock protein 83. [Violet
Japanese morning glory] {Pharbitis nil}, partial (96%)
Length = 2432
Score = 26.2 bits (56), Expect = 2.2
Identities = 10/37 (27%), Positives = 18/37 (48%)
Frame = +2
Query: 55 FCKTSLAHNGMYYVLSSICSFCCINLILYYFTTLLVC 91
+C+ L + Y + S+ CI++ +YF L C
Sbjct: 2168 YCRKKLMVSSYYV*MFSVMDDACISVYTFYFLLSLAC 2278
>TC81645 weakly similar to GP|22136076|gb|AAM91116.1 unknown protein
{Arabidopsis thaliana}, partial (28%)
Length = 917
Score = 26.2 bits (56), Expect = 2.2
Identities = 12/47 (25%), Positives = 26/47 (54%)
Frame = +1
Query: 45 SSDGGDVRWYFCKTSLAHNGMYYVLSSICSFCCINLILYYFTTLLVC 91
S+ G + +++ ++ + G Y +I ++ ++ LYY+ T LVC
Sbjct: 568 STGG*SLAYWWRNNNVQNTGQYCKYDTINTYSPQSIPLYYYVT*LVC 708
>AW127179 similar to GP|5679340|emb|C hypothetical protein {Galega
orientalis}, partial (25%)
Length = 447
Score = 26.2 bits (56), Expect = 2.2
Identities = 9/26 (34%), Positives = 14/26 (53%)
Frame = +2
Query: 56 CKTSLAHNGMYYVLSSICSFCCINLI 81
CK SL YYV+ +C C + ++
Sbjct: 368 CKNSL*RRMSYYVIEKMCIICYVRIL 445
>AW207949
Length = 425
Score = 26.2 bits (56), Expect = 2.2
Identities = 18/59 (30%), Positives = 28/59 (46%)
Frame = +2
Query: 17 NTPSNIARLKDVVEQSKARQKYLAHTNSSSDGGDVRWYFCKTSLAHNGMYYVLSSICSF 75
+T + ++R+K + +K L N SSD K +AHNG+YY + SF
Sbjct: 113 STMAEVSRVKG--NHAMNAKKVLQSVNKSSD---------KRCIAHNGLYYNIIGDGSF 256
>TC84590 similar to SP|P26415|NO14_PEA Nodulin 14 precursor (N-14)
(Fragment). [Garden pea] {Pisum sativum}, partial (24%)
Length = 447
Score = 26.2 bits (56), Expect = 2.2
Identities = 9/26 (34%), Positives = 14/26 (53%)
Frame = +3
Query: 56 CKTSLAHNGMYYVLSSICSFCCINLI 81
CK SL YYV+ +C C + ++
Sbjct: 243 CKNSL*RRMSYYVIEKMCIICYVRIL 320
>BE941074
Length = 668
Score = 25.8 bits (55), Expect = 2.9
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -1
Query: 66 YYVLSSICSFCCINLILYYF 85
Y++LSS FCC N + YF
Sbjct: 107 YFILSSSFWFCCFNNHITYF 48
>TC88212 similar to GP|10178030|dbj|BAB11513.
dbj|BAA90353.1~gene_id:MUG13.24~similar to unknown
protein {Arabidopsis thaliana}, partial (77%)
Length = 865
Score = 25.8 bits (55), Expect = 2.9
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = +1
Query: 69 LSSICSFCCINLILYYFTTLLVCIPFD 95
L+++C FCC NL + L+ I D
Sbjct: 691 LNAVCEFCCHNLYIKMLKKLIKKIDLD 771
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.323 0.137 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,176,393
Number of Sequences: 36976
Number of extensions: 71730
Number of successful extensions: 687
Number of sequences better than 10.0: 78
Number of HSP's better than 10.0 without gapping: 682
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 686
length of query: 95
length of database: 9,014,727
effective HSP length: 71
effective length of query: 24
effective length of database: 6,389,431
effective search space: 153346344
effective search space used: 153346344
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 51 (24.3 bits)
Medicago: description of AC124953.9