BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124952.8 - phase: 0 /pseudo
(819 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI309682 weakly similar to GP|14532612|g unknown protein {Arabid... 460 e-137
AW696428 weakly similar to GP|14532612|g unknown protein {Arabid... 219 4e-57
TC93552 122 5e-28
BF640789 weakly similar to GP|14532612|gb unknown protein {Arabi... 51 4e-15
TC86567 similar to GP|15384989|emb|CAC59823. Xaa-Pro aminopeptid... 35 0.089
CA922647 similar to GP|11191231|em putative helicase {Arabidopsi... 34 0.20
BG587124 30 4.9
BI308650 OMNI|NTL01EC0 tRNA nucleotidyl transferase {Escherichia... 29 6.4
TC93383 similar to PIR|T47786|T47786 hypothetical protein F17J16... 29 6.4
TC92960 weakly similar to GP|10177030|dbj|BAB10268. contains sim... 29 8.3
>BI309682 weakly similar to GP|14532612|g unknown protein {Arabidopsis
thaliana}, partial (16%)
Length = 805
Score = 460 bits (1183), Expect(3) = e-137
Identities = 232/240 (96%), Positives = 236/240 (97%)
Frame = +3
Query: 522 LSQISGGSQPSQNDHIIVSDDETEDAEAPFIQKLFGILRIYFVFLESIRRVSLGILAGVF 581
LSQISGGSQPSQNDHIIVSDDETEDAEAPFIQKLFGILRIYFVFLESIRRVSLGILAGVF
Sbjct: 42 LSQISGGSQPSQNDHIIVSDDETEDAEAPFIQKLFGILRIYFVFLESIRRVSLGILAGVF 221
Query: 582 IHTRSQSSKSPIIIMLSITSFMLFFMVLKKPFIKKKVQLVEIISLTCEVAFFATCFVLLK 641
IHTRSQSSKSPIIIMLSITSFMLFFMVLKKPFIKKKVQLVEIISLTCEVAFFATCFVLLK
Sbjct: 222 IHTRSQSSKSPIIIMLSITSFMLFFMVLKKPFIKKKVQLVEIISLTCEVAFFATCFVLLK 401
Query: 642 KDFSVRTETKFGIFMLVLFLVGYCSQIANEWYALYAQTKLLDPEEKSLFRGLKVASIGFV 701
KDFSVRTETKFGIFMLVLFLVGYCSQIANEWYALYAQTKLLDPEEKSLFRGLKVASIGFV
Sbjct: 402 KDFSVRTETKFGIFMLVLFLVGYCSQIANEWYALYAQTKLLDPEEKSLFRGLKVASIGFV 581
Query: 702 LYFIPQKWIKNLEKKLPQNGHANSETRDNALIAERCMHSGSRSSGTPDIPWLKRVRELAK 761
LYFIPQKWIKNLEKKLPQNGHANSETRDNALIAERCMHSGSRSSGTPDIPW+++ + K
Sbjct: 582 LYFIPQKWIKNLEKKLPQNGHANSETRDNALIAERCMHSGSRSSGTPDIPWVEKSKGTCK 761
Score = 45.1 bits (105), Expect(3) = e-137
Identities = 22/23 (95%), Positives = 22/23 (95%)
Frame = +1
Query: 753 LKRVRELAKGSFSKDRSGVQITD 775
LKRVRELAK SFSKDRSGVQITD
Sbjct: 736 LKRVRELAKSSFSKDRSGVQITD 804
Score = 26.6 bits (57), Expect(3) = e-137
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +2
Query: 512 EDLRGPPKYM 521
EDLRGPPKYM
Sbjct: 11 EDLRGPPKYM 40
>AW696428 weakly similar to GP|14532612|g unknown protein {Arabidopsis
thaliana}, partial (12%)
Length = 654
Score = 219 bits (557), Expect = 4e-57
Identities = 112/112 (100%), Positives = 112/112 (100%)
Frame = +1
Query: 100 DRRKVYVNIRTHVPEKLVQINSETRTVQATNDHNKLKVYLYFSAPVLNSSTEIMNSLKVS 159
DRRKVYVNIRTHVPEKLVQINSETRTVQATNDHNKLKVYLYFSAPVLNSSTEIMNSLKVS
Sbjct: 16 DRRKVYVNIRTHVPEKLVQINSETRTVQATNDHNKLKVYLYFSAPVLNSSTEIMNSLKVS 195
Query: 160 QGSLLPTSAENLGNRRFGFMIANISSTAIISVNFNSKSIITRQGTQVSPNAP 211
QGSLLPTSAENLGNRRFGFMIANISSTAIISVNFNSKSIITRQGTQVSPNAP
Sbjct: 196 QGSLLPTSAENLGNRRFGFMIANISSTAIISVNFNSKSIITRQGTQVSPNAP 351
Score = 46.6 bits (109), Expect = 4e-05
Identities = 22/22 (100%), Positives = 22/22 (100%)
Frame = +1
Query: 212 FTN*NGAHTLLSYKQMMILYLS 233
FTN*NGAHTLLSYKQMMILYLS
Sbjct: 589 FTN*NGAHTLLSYKQMMILYLS 654
>TC93552
Length = 386
Score = 122 bits (306), Expect(2) = 5e-28
Identities = 62/66 (93%), Positives = 65/66 (97%)
Frame = +1
Query: 383 QYFERNSEKNGTYGALVFPRFEIFLLFLALPGICKASTGLIRGGAPAAMAVGIILLIFVS 442
++ +RNSEKNGTYGALVFPRFEIFLLFLALPGICKASTGLIRGGAPAAMAVGIILLIFVS
Sbjct: 154 KFRKRNSEKNGTYGALVFPRFEIFLLFLALPGICKASTGLIRGGAPAAMAVGIILLIFVS 333
Query: 443 TVLLAL 448
TVLLAL
Sbjct: 334 TVLLAL 351
Score = 21.2 bits (43), Expect(2) = 5e-28
Identities = 9/10 (90%), Positives = 9/10 (90%)
Frame = +2
Query: 450 MFLSVGITFG 459
MFLS GITFG
Sbjct: 356 MFLSGGITFG 385
>BF640789 weakly similar to GP|14532612|gb unknown protein {Arabidopsis
thaliana}, partial (8%)
Length = 680
Score = 50.8 bits (120), Expect(2) = 4e-15
Identities = 26/27 (96%), Positives = 27/27 (99%)
Frame = +2
Query: 1 MEIILLKSAPMDIKDSLAVLAITGLLS 27
MEIILLKSAPMDIKDSLAVLAITGLL+
Sbjct: 413 MEIILLKSAPMDIKDSLAVLAITGLLT 493
Score = 49.3 bits (116), Expect(2) = 4e-15
Identities = 23/24 (95%), Positives = 23/24 (95%)
Frame = +1
Query: 27 SVNACNLLVYGAGQVIPSSFKILK 50
SVNACNLLVYGAGQVIPS FKILK
Sbjct: 607 SVNACNLLVYGAGQVIPSXFKILK 678
>TC86567 similar to GP|15384989|emb|CAC59823. Xaa-Pro aminopeptidase 1
{Lycopersicon esculentum}, partial (91%)
Length = 2223
Score = 35.4 bits (80), Expect = 0.089
Identities = 21/90 (23%), Positives = 46/90 (50%), Gaps = 4/90 (4%)
Frame = +1
Query: 38 AGQVIPSSFKILKPNLMYS----LLVSLSSTVQYSRAILVMDKNFCTDIAGNSFTRMPNS 93
AG+ + K L+ L ++++ V + I D +C + ++F + ++
Sbjct: 535 AGRSVTDKLKDLRKKLAQEHARGIVLTALDEVAWLYNIRGKDVAYCPVV--HAFAIVTSN 708
Query: 94 SVYIHIDRRKVYVNIRTHVPEKLVQINSET 123
S +I++D+RKV + ++TH+ E ++I T
Sbjct: 709 SAFIYVDKRKVSIEVKTHLEENGIEIKEYT 798
>CA922647 similar to GP|11191231|em putative helicase {Arabidopsis thaliana},
partial (21%)
Length = 711
Score = 34.3 bits (77), Expect = 0.20
Identities = 20/73 (27%), Positives = 36/73 (48%)
Frame = +3
Query: 263 ASLQSVDTFTRSSSFLIVDPARNLFRILCHIQVFALARWLSVKWPVEFYEFSRHLQWTIP 322
+S S++ F R+ S L V ++ + L R S++ P F+EF R + +
Sbjct: 342 SSKYSINRF-RNPSLLTVSVSQTIQHCLLARVTATFIRRRSLRKPTSFFEFDRTIDIIMA 518
Query: 323 SFSVPWESGPMSL 335
SFS+PW+ + +
Sbjct: 519 SFSLPWKPSTVEI 557
>BG587124
Length = 733
Score = 29.6 bits (65), Expect = 4.9
Identities = 31/103 (30%), Positives = 50/103 (48%), Gaps = 11/103 (10%)
Frame = -2
Query: 630 VAFFATCFV-LLKKDFSVRTETKFGIFMLVLFLVGYCSQIANEWY---ALYAQTKLLDPE 685
V F+ C V LL+++F T GI +LVL VG C+ + +Y A Y T +L
Sbjct: 300 VGFYICCVVVLLRRNFMW---TSGGILILVLNKVGLCALMRLSFYSSEAFYGDTVVLVRG 130
Query: 686 EKSLFR------GLKVASIGFVLYFIPQ-KWIKNLEKKLPQNG 721
++ F L V+ GF+L+ + ++N K+L + G
Sbjct: 129 LRTYFNNTGVTGSLVVSFSGFLLWAMENCSSLRNYNKELWKYG 1
>BI308650 OMNI|NTL01EC0 tRNA nucleotidyl transferase {Escherichia coli
K12-MG1655}, partial (43%)
Length = 765
Score = 29.3 bits (64), Expect = 6.4
Identities = 13/29 (44%), Positives = 21/29 (71%), Gaps = 1/29 (3%)
Frame = +2
Query: 783 RW-SGFWGNKRSGSSSSDYKPKPKKALDE 810
RW +G W RSG+++ D KP+ +++LDE
Sbjct: 68 RWRAGAWLAARSGTAAPDPKPQTQRSLDE 154
>TC93383 similar to PIR|T47786|T47786 hypothetical protein F17J16.90 -
Arabidopsis thaliana, partial (43%)
Length = 1038
Score = 29.3 bits (64), Expect = 6.4
Identities = 20/72 (27%), Positives = 35/72 (47%)
Frame = +1
Query: 475 HWYQELIRVTLGPGKRGQWTWKEKAKSVYLTIFGPLFEDLRGPPKYMLSQISGGSQPSQN 534
+W++E+ L P +KAK++ L++ ED++ + +L I + P N
Sbjct: 583 NWFKEMALNGLLPD--------QKAKNILLSL-AKTEEDIKEANELVLHSIEINNLPKVN 735
Query: 535 DHIIVSDDETED 546
DH DD+ ED
Sbjct: 736 DHEEDDDDDDED 771
>TC92960 weakly similar to GP|10177030|dbj|BAB10268. contains similarity to
protein kinase~gene_id:MQJ2.14 {Arabidopsis thaliana},
partial (21%)
Length = 841
Score = 28.9 bits (63), Expect = 8.3
Identities = 27/88 (30%), Positives = 42/88 (47%), Gaps = 3/88 (3%)
Frame = +2
Query: 93 SSVYIHIDRRKVYVNIRTHVPEKLVQINSETRTVQATNDHNKLKVYLYFSAPVLNSSTEI 152
+SV++H +V+ + HV + SET TV TN H+ L + L + S T I
Sbjct: 38 TSVFLH-----TFVSTKKHVQIQ----KSETTTVSITNHHHHLLLLLLHTFSPNPSHTFI 190
Query: 153 MNSLKV---SQGSLLPTSAENLGNRRFG 177
+ + K+ S G L+ T + RR G
Sbjct: 191 L-TFKINGFSIGMLVSTQQQQQRRRRHG 271
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.325 0.138 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,931,595
Number of Sequences: 36976
Number of extensions: 359924
Number of successful extensions: 2700
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1996
Number of HSP's successfully gapped in prelim test: 102
Number of HSP's that attempted gapping in prelim test: 667
Number of HSP's gapped (non-prelim): 2155
length of query: 819
length of database: 9,014,727
effective HSP length: 104
effective length of query: 715
effective length of database: 5,169,223
effective search space: 3695994445
effective search space used: 3695994445
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 62 (28.5 bits)
Medicago: description of AC124952.8