BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124215.9 - phase: 0
(475 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86625 similar to GP|16226834|gb|AAL16275.1 AT3g54190/F24B22_15... 598 e-171
TC87343 homologue to GP|16226834|gb|AAL16275.1 AT3g54190/F24B22_... 552 e-157
TC79700 similar to GP|16226834|gb|AAL16275.1 AT3g54190/F24B22_15... 510 e-145
TC86626 similar to GP|16226834|gb|AAL16275.1 AT3g54190/F24B22_15... 111 5e-25
TC92752 similar to GP|16226834|gb|AAL16275.1 AT3g54190/F24B22_15... 86 2e-21
TC77757 weakly similar to PIR|A96610|A96610 probable pectinacety... 30 2.6
TC78162 29 3.4
TC78160 similar to GP|15293281|gb|AAK93751.1 unknown protein {Ar... 29 3.4
TC78161 29 3.4
TC80892 weakly similar to GP|23429649|gb|AAN17752.1 ovate protei... 29 4.5
TC76962 similar to GP|10241935|dbj|BAB13711. elicitor inducible ... 28 5.9
TC82106 similar to PIR|T49191|T49191 hypothetical protein MAA21.... 28 7.7
>TC86625 similar to GP|16226834|gb|AAL16275.1 AT3g54190/F24B22_150
{Arabidopsis thaliana}, partial (80%)
Length = 1447
Score = 598 bits (1542), Expect(2) = e-171
Identities = 296/361 (81%), Positives = 328/361 (89%)
Frame = +1
Query: 36 NIIKKLQTREISPKPHRSFVAATSPHRFQNMRLTHQFDTHDPKHHSSPSPFLPFLMKRTK 95
N +KKLQ REIS K R+F + RF+N+RL ++DTHDPK SS LPFL KR+K
Sbjct: 328 NSVKKLQRREISSKRDRAFSMTDAQERFRNIRLQEEYDTHDPKGPSSV--VLPFLRKRSK 501
Query: 96 VVEIVAAKNIVFALAHSGLCAAFSRETNERICFLNICPDEVIRSLFYNKNNDSLITVSVY 155
++EIVAA++IVFALA SG+CAAFSRETN+RICFLN+ PDEVIRSLFYNKNNDSLITVSVY
Sbjct: 502 IIEIVAAQDIVFALAQSGVCAAFSRETNQRICFLNVSPDEVIRSLFYNKNNDSLITVSVY 681
Query: 156 ASENFSSLKCRSTRIEYIKRAKPDAGFPLFQSESLKWPGFVEFDDVNAKVLTYSAQDSIY 215
AS+++SSLKCRSTRIEYI+R KPDAGF LF+SESLKWPGFVEFDDVN KVLTYSAQDSIY
Sbjct: 682 ASDSYSSLKCRSTRIEYIRRGKPDAGFALFESESLKWPGFVEFDDVNGKVLTYSAQDSIY 861
Query: 216 KVFDLKNYTLLYSISDRNVQEIKISPGIMLLIFNRASGHIPLKIISIEDGTVLKAFNHLL 275
KVFDLKNYT+LYSISD+NVQEIKISPGIMLLIF +AS H+PLKI+SIEDGTVLK+FNHLL
Sbjct: 862 KVFDLKNYTMLYSISDKNVQEIKISPGIMLLIFAKASSHVPLKILSIEDGTVLKSFNHLL 1041
Query: 276 HRNKKVDFIEQFNEKLLVKQENENLQILDVRSSELMEVSRTEFMTPSAFIFLYENQLFLT 335
+RNKKVDFIEQFNEKLLVKQENENLQILDVR+ EL EVSR+EFMTPSAFIFLYENQLFLT
Sbjct: 1042YRNKKVDFIEQFNEKLLVKQENENLQILDVRTFELTEVSRSEFMTPSAFIFLYENQLFLT 1221
Query: 336 FRNRTVSVWNFRGELVTSFEDHLLWHPDCNTNNIYITSDQDLIISYCKAESEDQWMEANA 395
FRNRTV+VWNFRGELVTSFEDHLLWHPDCNTNNIYITSDQDLIISYCKA+S+D E NA
Sbjct: 1222FRNRTVAVWNFRGELVTSFEDHLLWHPDCNTNNIYITSDQDLIISYCKADSDDSLTEGNA 1401
Query: 396 G 396
G
Sbjct: 1402G 1404
Score = 21.6 bits (44), Expect(2) = e-171
Identities = 8/10 (80%), Positives = 10/10 (100%)
Frame = +3
Query: 400 VSNILTGKCV 409
VSNIL+GKC+
Sbjct: 1416 VSNILSGKCL 1445
>TC87343 homologue to GP|16226834|gb|AAL16275.1 AT3g54190/F24B22_150
{Arabidopsis thaliana}, partial (64%)
Length = 1328
Score = 552 bits (1422), Expect = e-157
Identities = 271/338 (80%), Positives = 304/338 (89%), Gaps = 9/338 (2%)
Frame = +3
Query: 147 DSLITVSVYASENFSSLKCRSTRIEYIKRAKPDAGFPLFQSESLKWPGFVEFDDVNAKVL 206
DSLITVSVYAS+++SSLKCRSTRIEYI+R +PDAGF LF+SESLKWPGFVEFDDVN KVL
Sbjct: 3 DSLITVSVYASDSYSSLKCRSTRIEYIRRVQPDAGFALFESESLKWPGFVEFDDVNGKVL 182
Query: 207 TYSAQDSIYKVFDLKNYTLLYSISDRNVQEIKISPGIMLLIFNRASGHIPLKIISIEDGT 266
TYSAQDSIYKVFDLKNY++LYS++D+NVQEIKISPGIMLLI+ +AS H+PLKI+SIEDGT
Sbjct: 183 TYSAQDSIYKVFDLKNYSMLYSVADKNVQEIKISPGIMLLIYTKASSHVPLKILSIEDGT 362
Query: 267 VLKAFNHLLHRNKKVDFIEQFNEKLLVKQENENLQILDVRSSELMEVSRTEFMTPSAFIF 326
VLK+FNHL+HRN KVDFIEQFNEKLLVKQENENLQILDVR+ EL EVS+TEFMTPSAFIF
Sbjct: 363 VLKSFNHLIHRNSKVDFIEQFNEKLLVKQENENLQILDVRTFELTEVSKTEFMTPSAFIF 542
Query: 327 LYENQLFLTFRNRTVSVWNFRGELVTSFEDHLLWHPDCNTNNIYITSDQDLIISYCKAES 386
LYENQLFLTFRNRTV+VWNFRGELVTSFEDHLLWHPDCNTNNIYITSDQDLIISYCKA+S
Sbjct: 543 LYENQLFLTFRNRTVAVWNFRGELVTSFEDHLLWHPDCNTNNIYITSDQDLIISYCKADS 722
Query: 387 EDQWMEANAGSINVSNILTGKCVAKINAANC--ISKVDDCSSTCSC-------KHTDSSQ 437
++ E NAGSIN+SNILTGKC+AKI A+N I+K +C CS + SS+
Sbjct: 723 DESLSEGNAGSINISNILTGKCLAKIRASNSFPIAKECNCGDDCSTDGCNSRKRKQASSR 902
Query: 438 LRSSVAEALEDITALFYDEDRNEIYTGNRHGFVHVWSN 475
+R++V EALEDITALFYDEDRNEIYTGNRHG VHVWSN
Sbjct: 903 IRNTVREALEDITALFYDEDRNEIYTGNRHGLVHVWSN 1016
>TC79700 similar to GP|16226834|gb|AAL16275.1 AT3g54190/F24B22_150
{Arabidopsis thaliana}, partial (79%)
Length = 1644
Score = 510 bits (1314), Expect = e-145
Identities = 256/266 (96%), Positives = 258/266 (96%), Gaps = 1/266 (0%)
Frame = +3
Query: 1 MDERRRGVWSCRRIVATKGESSNNGSKPNIKINNKNIIKKLQTREISPKPHRSFVAATSP 60
MDERRRGVWSCRRIVATKGESSNNGSKPNIKINNKNIIKKLQTREISPKPHRSFVAATSP
Sbjct: 39 MDERRRGVWSCRRIVATKGESSNNGSKPNIKINNKNIIKKLQTREISPKPHRSFVAATSP 218
Query: 61 HRFQNMRLTHQFDTHDPKHHSSPSPFLPFLMKRTKVVEIVAAKNIVFALAHSGLCAAFSR 120
HRFQNMRLTHQFDTHDPKHHSSPSPFLPFLMKRTKVVEIVAAKNIVFALAHSGLCAAFSR
Sbjct: 219 HRFQNMRLTHQFDTHDPKHHSSPSPFLPFLMKRTKVVEIVAAKNIVFALAHSGLCAAFSR 398
Query: 121 ETNERICFLNICPDEVIRSLFYNKNNDSLITVSVYASENFSSLKCRSTRIEYIKRAKPDA 180
ETNERICFLNICPDEVIRSLFYNKNNDSLITVSVYASENFSSLKCRSTRIEYIKRAKPDA
Sbjct: 399 ETNERICFLNICPDEVIRSLFYNKNNDSLITVSVYASENFSSLKCRSTRIEYIKRAKPDA 578
Query: 181 GFPLFQSESLKWPGFVEFDDVNAKVLTYSAQDSIYKVFDLKNYTLLYSISDRNVQEIKIS 240
GFPLFQSESLKWPGFVEFDDVNAKVLTYSAQDSIYKVFDLKNYTLLYSISDRNVQEIKIS
Sbjct: 579 GFPLFQSESLKWPGFVEFDDVNAKVLTYSAQDSIYKVFDLKNYTLLYSISDRNVQEIKIS 758
Query: 241 PGIMLLIFNRAS-GHIPLKIISIEDG 265
PGIMLLIFNRA + K+ IEDG
Sbjct: 759 PGIMLLIFNRAKWSYSH*KLYPIEDG 836
Score = 433 bits (1114), Expect = e-122
Identities = 214/225 (95%), Positives = 216/225 (95%), Gaps = 1/225 (0%)
Frame = +1
Query: 252 SGHIPLK-IISIEDGTVLKAFNHLLHRNKKVDFIEQFNEKLLVKQENENLQILDVRSSEL 310
SGHIP + I + GTVLKAFNHLLHRNKKVDFIEQFNEKLLVKQENENLQILDVRSSEL
Sbjct: 793 SGHIPTENYIPLRMGTVLKAFNHLLHRNKKVDFIEQFNEKLLVKQENENLQILDVRSSEL 972
Query: 311 MEVSRTEFMTPSAFIFLYENQLFLTFRNRTVSVWNFRGELVTSFEDHLLWHPDCNTNNIY 370
MEVSRTEFMTPSAFIFLYENQLFLTFRNRTVSVWNFRGELVTS EDHLLWHP CNTNNIY
Sbjct: 973 MEVSRTEFMTPSAFIFLYENQLFLTFRNRTVSVWNFRGELVTSLEDHLLWHPHCNTNNIY 1152
Query: 371 ITSDQDLIISYCKAESEDQWMEANAGSINVSNILTGKCVAKINAANCISKVDDCSSTCSC 430
ITSDQDLIISYCKAESEDQWMEANAGSI VSNILTGKCVAKINAANCISKVDDCSSTCSC
Sbjct: 1153 ITSDQDLIISYCKAESEDQWMEANAGSITVSNILTGKCVAKINAANCISKVDDCSSTCSC 1332
Query: 431 KHTDSSQLRSSVAEALEDITALFYDEDRNEIYTGNRHGFVHVWSN 475
KHTDSSQLRSSVAEALEDITALFYDEDRNEIYTGNRHGFVHVWSN
Sbjct: 1333 KHTDSSQLRSSVAEALEDITALFYDEDRNEIYTGNRHGFVHVWSN 1467
>TC86626 similar to GP|16226834|gb|AAL16275.1 AT3g54190/F24B22_150
{Arabidopsis thaliana}, partial (13%)
Length = 596
Score = 111 bits (278), Expect = 5e-25
Identities = 61/89 (68%), Positives = 68/89 (75%), Gaps = 9/89 (10%)
Frame = +1
Query: 396 GSINVSNILTGKCVAKINAANCISKVDDCSSTC------SC---KHTDSSQLRSSVAEAL 446
GSINVSNIL+GKC+AKI A+N S +DD S C SC K S++RS+VAEAL
Sbjct: 1 GSINVSNILSGKCLAKIRASNS-SPMDDKCSCCDSSSGGSCNSRKRKHVSKMRSTVAEAL 177
Query: 447 EDITALFYDEDRNEIYTGNRHGFVHVWSN 475
EDITALFYDEDRNEIYTGNRHG VHVWSN
Sbjct: 178 EDITALFYDEDRNEIYTGNRHGLVHVWSN 264
>TC92752 similar to GP|16226834|gb|AAL16275.1 AT3g54190/F24B22_150
{Arabidopsis thaliana}, partial (20%)
Length = 661
Score = 86.3 bits (212), Expect(2) = 2e-21
Identities = 43/63 (68%), Positives = 53/63 (83%)
Frame = +1
Query: 68 LTHQFDTHDPKHHSSPSPFLPFLMKRTKVVEIVAAKNIVFALAHSGLCAAFSRETNERIC 127
L ++DT+DPK SS LPFL KR+K++EIVAA++IVFALA SG+CAAF+RETNERIC
Sbjct: 475 LQEEYDTYDPKGPSSI--VLPFLRKRSKIIEIVAAQDIVFALAQSGVCAAFNRETNERIC 648
Query: 128 FLN 130
FLN
Sbjct: 649 FLN 657
Score = 34.3 bits (77), Expect(2) = 2e-21
Identities = 21/56 (37%), Positives = 28/56 (49%)
Frame = +3
Query: 12 RRIVATKGESSNNGSKPNIKINNKNIIKKLQTREISPKPHRSFVAATSPHRFQNMR 67
RR+VA K + I N ++KLQ REIS K R F + RF+N+R
Sbjct: 321 RRVVAKKKQGRGGAGDGFI-----NSVRKLQRREISTKSVRGFSITDAQERFRNIR 473
>TC77757 weakly similar to PIR|A96610|A96610 probable pectinacetylesterase
precursor T8L23.6 [imported] - Arabidopsis thaliana,
partial (86%)
Length = 1536
Score = 29.6 bits (65), Expect = 2.6
Identities = 21/66 (31%), Positives = 25/66 (37%)
Frame = +3
Query: 310 LMEVSRTEFMTPSAFIFLYENQLFLTFRNRTVSVWNFRGELVTSFEDHLLWHPDCNTNNI 369
L ++ T P + E LFL N VW R L S D L DC +NN
Sbjct: 972 LNKMDPTSCFFPQNVVEHVETPLFLL--NAAYDVWQIRASLAPSTADPLGSWNDCKSNNA 1145
Query: 370 YITSDQ 375
S Q
Sbjct: 1146 NCNSSQ 1163
>TC78162
Length = 727
Score = 29.3 bits (64), Expect = 3.4
Identities = 10/31 (32%), Positives = 17/31 (54%)
Frame = +2
Query: 360 WHPDCNTNNIYITSDQDLIISYCKAESEDQW 390
W+ C TN+ + DQDL+ + E+ +W
Sbjct: 140 WYYGCGTNDFVVPGDQDLMARHPSPENWSKW 232
>TC78160 similar to GP|15293281|gb|AAK93751.1 unknown protein {Arabidopsis
thaliana}, partial (6%)
Length = 1630
Score = 29.3 bits (64), Expect = 3.4
Identities = 10/31 (32%), Positives = 17/31 (54%)
Frame = +1
Query: 360 WHPDCNTNNIYITSDQDLIISYCKAESEDQW 390
W+ C TN+ + DQDL+ + E+ +W
Sbjct: 157 WYYGCGTNDFVVPGDQDLMARHPSPENWSKW 249
>TC78161
Length = 819
Score = 29.3 bits (64), Expect = 3.4
Identities = 10/31 (32%), Positives = 17/31 (54%)
Frame = +2
Query: 360 WHPDCNTNNIYITSDQDLIISYCKAESEDQW 390
W+ C TN+ + DQDL+ + E+ +W
Sbjct: 155 WYYGCGTNDFVVPGDQDLMARHPSPENWSKW 247
>TC80892 weakly similar to GP|23429649|gb|AAN17752.1 ovate protein
{Lycopersicon esculentum}, partial (16%)
Length = 681
Score = 28.9 bits (63), Expect = 4.5
Identities = 19/55 (34%), Positives = 27/55 (48%)
Frame = +3
Query: 22 SNNGSKPNIKINNKNIIKKLQTREISPKPHRSFVAATSPHRFQNMRLTHQFDTHD 76
S+N S IKI N++ E S KP SF T+P+ + +H +THD
Sbjct: 15 SSNKSCGCIKIKPSNVL------EPSQKPKISFNQNTNPNTLTSPTTSHDANTHD 161
>TC76962 similar to GP|10241935|dbj|BAB13711. elicitor inducible protein
{Nicotiana tabacum}, partial (76%)
Length = 1024
Score = 28.5 bits (62), Expect = 5.9
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
Frame = -1
Query: 22 SNNGSKPNIKINNKNIIKKLQTR--EISPKPHRSFVAATSPHRFQNMRLTHQF 72
+NNGS IK+NN+ K+ +T +P RS ++ + HR R H +
Sbjct: 751 NNNGSTQAIKLNNQTK*KERKTTILNTTPTATRSHISYGNKHRKSRPREQHTY 593
>TC82106 similar to PIR|T49191|T49191 hypothetical protein MAA21.130 -
Arabidopsis thaliana, partial (5%)
Length = 990
Score = 28.1 bits (61), Expect = 7.7
Identities = 17/48 (35%), Positives = 23/48 (47%), Gaps = 1/48 (2%)
Frame = -2
Query: 41 LQTREISPKP-HRSFVAATSPHRFQNMRLTHQFDTHDPKHHSSPSPFL 87
L TR + P HR + S Q H++ H P H +SPSP+L
Sbjct: 734 LDTRPLFPPSLHRQHHSRLSLQLTQVCYHLHRYPPHAPLH*ASPSPYL 591
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.134 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,409,834
Number of Sequences: 36976
Number of extensions: 223662
Number of successful extensions: 1469
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 1456
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1465
length of query: 475
length of database: 9,014,727
effective HSP length: 100
effective length of query: 375
effective length of database: 5,317,127
effective search space: 1993922625
effective search space used: 1993922625
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Medicago: description of AC124215.9