BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124215.6 - phase: 0 /pseudo
(679 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imp... 218 9e-57
BE248682 similar to GP|18568269|gb putative gag-pol polyprotein ... 121 8e-28
BG586266 similar to GP|7267666|em RNA-directed DNA polymerase-li... 119 4e-27
TC80819 weakly similar to GP|10140689|gb|AAG13524.1 putative non... 63 8e-20
TC77455 similar to GP|22335695|dbj|BAC10549. nine-cis-epoxycarot... 47 3e-12
BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgari... 60 2e-09
BQ148771 60 3e-09
BG647708 weakly similar to GP|13786450|gb| putative reverse tran... 59 8e-09
TC91765 weakly similar to GP|19881779|gb|AAM01180.1 Putative ret... 39 2e-08
AW774658 similar to GP|2808681|emb| Hcr9-4B {Lycopersicon hirsut... 40 2e-07
TC83624 homologue to PIR|G84581|G84581 copia-like retroelement p... 46 5e-05
CB891696 43 5e-04
TC82520 42 6e-04
BF006686 42 0.001
TC82948 41 0.001
BG584442 40 0.003
TC82763 36 0.042
AJ497561 weakly similar to GP|10140689|gb putative non-LTR retro... 35 0.12
BF650593 29 0.84
AI737603 32 1.0
>TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imported] -
Arabidopsis thaliana, partial (6%)
Length = 951
Score = 218 bits (556), Expect(2) = 9e-57
Identities = 127/269 (47%), Positives = 166/269 (61%), Gaps = 1/269 (0%)
Frame = +2
Query: 1 DVQSAFIKGRQILDGILVANEVVDDARKRKKDLLLFKVDFEKAYDSVD*NYLEEVM-VKM 59
D QSAF+K RQIL+ + + + + + + K ++ + + V M
Sbjct: 203 DAQSAFVKNRQILEMVFL*QMRLWMRLRN*RKIFCCLRWILKRLITLSIGLIWILF*VGM 382
Query: 60 GFPTLWRKWIKKCVGTATASVLVNGSPTYEFSLHRGLRQGDPLSPFLFLLAAEGFHVLME 119
F LWRKWIK+CV TAT SVLVNGSPT +VLM+
Sbjct: 383 SFLVLWRKWIKECVSTATTSVLVNGSPT---------------------------NVLMK 481
Query: 120 ALVVNNLFNGYKVGSHEMVGVTHLQFADDTIILCDKSWANIRALRAILLLFQELSGLKVN 179
+LV LF Y G V V+HLQFA+DT++L K+WANIRALRA L++F +SGLKVN
Sbjct: 482 SLVQTQLFTRYSFGVVNPVVVSHLQFANDTLLLETKNWANIRALRAALVIF*AMSGLKVN 661
Query: 180 FSKSLFVGVNVHGSWLAEAATVLNCNAGSIPFLYLGLPIGGNASRMVFWKPLINRINSRL 239
F KS V VN+ SWL+EAA+VL+ G +PFLYLG+PI GN+ R+ FW+P++NRI +RL
Sbjct: 662 FHKSGLVCVNIAPSWLSEAASVLSWKVGKVPFLYLGMPIEGNSRRLSFWEPIVNRIKARL 841
Query: 240 SSWKSKHLSLGGRLVLLKSVLSSLPVYAL 268
+ W S+ LS GGRLVLLKSVL+SL VYAL
Sbjct: 842 TGWNSRFLSFGGRLVLLKSVLTSLSVYAL 928
Score = 20.8 bits (42), Expect(2) = 9e-57
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +1
Query: 269 SFFKAPSG 276
SFFKAPSG
Sbjct: 928 SFFKAPSG 951
>BE248682 similar to GP|18568269|gb putative gag-pol polyprotein {Zea mays},
partial (1%)
Length = 441
Score = 121 bits (304), Expect = 8e-28
Identities = 66/131 (50%), Positives = 85/131 (64%)
Frame = +3
Query: 89 EFSLHRGLRQGDPLSPFLFLLAAEGFHVLMEALVVNNLFNGYKVGSHEMVGVTHLQFADD 148
E S+ RGL+QGDPL+PFLFLL AEG LM+ V NLF G+ V V+HLQ+ADD
Sbjct: 9 EISVQRGLKQGDPLAPFLFLLVAEGISGLMKNAVNRNLFQGFDV-KRGGTRVSHLQYADD 185
Query: 149 TIILCDKSWANIRALRAILLLFQELSGLKVNFSKSLFVGVNVHGSWLAEAATVLNCNAGS 208
T+ + + N+ L+A+L F+ SGLKVNF KS +G+NV ++ A LNC S
Sbjct: 186 TLCIGMPTVDNLWTLKALLQGFEMASGLKVNFHKSSLIGINVPRDFMEAACRFLNCREES 365
Query: 209 IPFLYLGLPIG 219
IPF+YLGLP G
Sbjct: 366 IPFIYLGLPGG 398
>BG586266 similar to GP|7267666|em RNA-directed DNA polymerase-like protein
{Arabidopsis thaliana}, partial (18%)
Length = 789
Score = 119 bits (298), Expect = 4e-27
Identities = 67/184 (36%), Positives = 102/184 (55%), Gaps = 3/184 (1%)
Frame = -3
Query: 3 QSAFIKGRQILDGILVANEVVDDARK---RKKDLLLFKVDFEKAYDSVD*NYLEEVMVKM 59
QSAF+ GR I D +L+ ++++ R+ +K + K D KAYD + N+L EV+ ++
Sbjct: 670 QSAFVPGRAISDNVLITHKILHYLRQSGAKKHVSMAVKTDMTKAYDRIAWNFLREVLTRL 491
Query: 60 GFPTLWRKWIKKCVGTATASVLVNGSPTYEFSLHRGLRQGDPLSPFLFLLAAEGFHVLME 119
GF +W WI +CV T + S L+NG P RGLRQGDPLSP+LF+L E L +
Sbjct: 490 GFHGIWISWIMECVSTVSYSFLINGGPQGRVLPSRGLRQGDPLSPYLFILCTEVLSGLCQ 311
Query: 120 ALVVNNLFNGYKVGSHEMVGVTHLQFADDTIILCDKSWANIRALRAILLLFQELSGLKVN 179
+ G KV + + HL FADDT+ + ++ L +I+ ++ SG +N
Sbjct: 310 QALRKGTLPGVKV-ARNCPPINHLLFADDTMFFGKSNASSCAILLSIMDKYRAASGRCIN 134
Query: 180 FSKS 183
+KS
Sbjct: 133 *TKS 122
>TC80819 weakly similar to GP|10140689|gb|AAG13524.1 putative non-LTR
retroelement reverse transcriptase {Oryza sativa
(japonica cultivar-group)}, partial (2%)
Length = 1262
Score = 62.8 bits (151), Expect(2) = 8e-20
Identities = 30/74 (40%), Positives = 42/74 (56%), Gaps = 6/74 (8%)
Frame = +3
Query: 361 GGHLCSGGRNESMWWRNIVALHS---EG---WFQNHVSRSLGDGSSVLFWTDV*VGELSL 414
GG LC GGR SMWW+ I + EG WF+ ++ +GDG FW D G++ L
Sbjct: 144 GGWLCEGGRQSSMWWKTICKVREGVGEGVGNWFEENIRMVVGDGRDAFFWYDTWAGDVPL 323
Query: 415 RDRFSRLYDLSVLK 428
R ++ RL DL++ K
Sbjct: 324 RLKYPRLLDLAMDK 365
Score = 52.8 bits (125), Expect(2) = 8e-20
Identities = 21/34 (61%), Positives = 26/34 (75%)
Frame = +1
Query: 329 FNLALLGKWCWRVLTERDSLWFRVLAVRNRVEGG 362
FNL+LLGKWCWR+L +++ LW RVL R EGG
Sbjct: 49 FNLSLLGKWCWRLLVDKEGLWHRVLKARYGEEGG 150
>TC77455 similar to GP|22335695|dbj|BAC10549. nine-cis-epoxycarotenoid
dioxygenase1 {Pisum sativum}, partial (43%)
Length = 1865
Score = 46.6 bits (109), Expect(2) = 3e-12
Identities = 26/74 (35%), Positives = 37/74 (49%), Gaps = 8/74 (10%)
Frame = -2
Query: 367 GGRN----ESMWWRNIVALHSEG---WFQNHVSRSLGDGSSVLFWTDV*VGELSLRDRFS 419
GG N S WW+++++L G WF + R +GDG S FW D + LR+ F
Sbjct: 805 GGENWPAYASRWWKDLMSLEEVGRVRWFPRELIRKVGDGRSSFFWKDAWDSSVPLRESFP 626
Query: 420 R-LYDLSVLKGGVG 432
R + +LK G G
Sbjct: 625 RAFFPYRLLKMGCG 584
Score = 43.5 bits (101), Expect(2) = 3e-12
Identities = 20/41 (48%), Positives = 27/41 (65%)
Frame = -3
Query: 313 CRSQEVGGLEVRRIKEFNLALLGKWCWRVLTERDSLWFRVL 353
C + GGL VR I+ N++LL KW WR+L ++ SLW VL
Sbjct: 969 CLPRCKGGLGVRDIRLVNVSLLAKWWWRLLQDQSSLWKEVL 847
>BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgaris}, partial
(69%)
Length = 616
Score = 60.5 bits (145), Expect = 2e-09
Identities = 31/86 (36%), Positives = 53/86 (61%), Gaps = 1/86 (1%)
Frame = +3
Query: 3 QSAFIKGRQILDGILVANEVVDD-ARKRKKDLLLFKVDFEKAYDSVD*NYLEEVMVKMGF 61
QSAF+KGR I D I++++E+V +RK + K+D KAYDS + +++ +M+++GF
Sbjct: 351 QSAFVKGRVIFDNIILSHELVKSYSRKGISPRCMVKIDLXKAYDSXEWPFIKHLMLELGF 530
Query: 62 PTLWRKWIKKCVGTATASVLVNGSPT 87
P + W+ + TA+ + NG T
Sbjct: 531 PYKFVNWVMAXLTTASYTFNXNGDLT 608
>BQ148771
Length = 680
Score = 60.1 bits (144), Expect = 3e-09
Identities = 33/120 (27%), Positives = 61/120 (50%)
Frame = -3
Query: 234 RINSRLSSWKSKHLSLGGRLVLLKSVLSSLPVYALSFFKAPSGIVSSIESILNNFFFWGG 293
+++ L++WK+ HLSL R+ L KSV+ ++P+Y + P + I+ + F
Sbjct: 588 QVHVMLANWKANHLSLARRVTLAKSVIEAVPLYPMMTTIIPKACIEEIQKLQRKFV---- 421
Query: 294 WGGGSADHRKIIWVDWNSVCRSQEVGGLEVRRIKEFNLALLGKWCWRVLTERDSLWFRVL 353
WG R+ V W ++ + + + GL +RR+ N A + K W + + +SL V+
Sbjct: 420 WGDTEVS-RRYHAVGWETMSKPKTIYGLGLRRLDVMNKACIMKLGWSIYSGSNSLCTEVM 244
>BG647708 weakly similar to GP|13786450|gb| putative reverse transcriptase
{Oryza sativa}, partial (9%)
Length = 708
Score = 58.5 bits (140), Expect = 8e-09
Identities = 33/90 (36%), Positives = 49/90 (53%)
Frame = +1
Query: 94 RGLRQGDPLSPFLFLLAAEGFHVLMEALVVNNLFNGYKVGSHEMVGVTHLQFADDTIILC 153
+GLRQGDPLSP+LF+L A L++ +G +V + +THL FADD+++
Sbjct: 10 KGLRQGDPLSPYLFILCANVLSGLLKREGNKQNLHGIQVARSDPK-ITHLLFADDSLLFA 186
Query: 154 DKSWANIRALRAILLLFQELSGLKVNFSKS 183
+ + +L +Q SG VNF KS
Sbjct: 187 RANLTEAATIMQVLHSYQSASGQLVNFEKS 276
>TC91765 weakly similar to GP|19881779|gb|AAM01180.1 Putative retroelement
{Oryza sativa (japonica cultivar-group)}, partial (1%)
Length = 625
Score = 38.5 bits (88), Expect(2) = 2e-08
Identities = 15/28 (53%), Positives = 21/28 (74%)
Frame = +1
Query: 42 KAYDSVD*NYLEEVMVKMGFPTLWRKWI 69
K Y+ VD*+YL+E+M+KMGF W W+
Sbjct: 13 KVYNRVD*DYLKEIMIKMGFNNRWIYWM 96
Score = 38.1 bits (87), Expect(2) = 2e-08
Identities = 19/47 (40%), Positives = 26/47 (54%)
Frame = +2
Query: 72 CVGTATASVLVNGSPTYEFSLHRGLRQGDPLSPFLFLLAAEGFHVLM 118
CV + VLVN RGL+QGD LSP++F++ EG L+
Sbjct: 104 CVESNDYYVLVNNDAVDPIIPSRGLQQGDHLSPYIFIICVEGLSFLI 244
>AW774658 similar to GP|2808681|emb| Hcr9-4B {Lycopersicon hirsutum}, partial
(4%)
Length = 665
Score = 40.0 bits (92), Expect(2) = 2e-07
Identities = 17/39 (43%), Positives = 26/39 (66%)
Frame = -1
Query: 121 LVVNNLFNGYKVGSHEMVGVTHLQFADDTIILCDKSWAN 159
L+ +++ +G H + +HLQFADDT++L KSWAN
Sbjct: 443 LLSHSICLNLSIGMHSLTVFSHLQFADDTLLLGVKSWAN 327
Score = 33.1 bits (74), Expect(2) = 2e-07
Identities = 14/28 (50%), Positives = 22/28 (78%)
Frame = -3
Query: 161 RALRAILLLFQELSGLKVNFSKSLFVGV 188
RALR+IL++F+ +SGLKVN + + + V
Sbjct: 327 RALRSILVIFENMSGLKVNLREEVIIYV 244
>TC83624 homologue to PIR|G84581|G84581 copia-like retroelement pol
polyprotein [imported] - Arabidopsis thaliana, partial
(1%)
Length = 831
Score = 45.8 bits (107), Expect = 5e-05
Identities = 20/56 (35%), Positives = 33/56 (58%)
Frame = +1
Query: 180 FSKSLFVGVNVHGSWLAEAATVLNCNAGSIPFLYLGLPIGGNASRMVFWKPLINRI 235
FS+ F+ +N+ S++ + L CN +PF +LGLPIG N R KP+++ +
Sbjct: 256 FSRVNFMALNLEESFVEASPNFLLCNVNEVPFCFLGLPIGANPKRSSTRKPVLDSL 423
>CB891696
Length = 638
Score = 42.7 bits (99), Expect = 5e-04
Identities = 26/84 (30%), Positives = 42/84 (49%)
Frame = +1
Query: 159 NIRALRAILLLFQELSGLKVNFSKSLFVGVNVHGSWLAEAATVLNCNAGSIPFLYLGLPI 218
NI ++ I+ F+ S L VNF KS + +NV G + + C + F YLG+ +
Sbjct: 10 NILTMKTIVSYFELASSLWVNFLKSGLINLNVIGHF*GW*NIYIKCKVH*VIFKYLGILV 189
Query: 219 GGNASRMVFWKPLINRINSRLSSW 242
G N R+ + L+ + + L SW
Sbjct: 190 GENPCRVNM*ELLLKLLTN*LGSW 261
>TC82520
Length = 833
Score = 42.4 bits (98), Expect = 6e-04
Identities = 40/108 (37%), Positives = 48/108 (44%)
Frame = +3
Query: 568 IYFYIVIFLDRF*ITFSNGLE*LR*CLKM*HVILSSSLF*ALSLSRGNLFFR*YGLRLCG 627
I FYIVIFL F + F G L * + L + LF + G L G
Sbjct: 342 ICFYIVIFLALFGLMFCVGYIFCWFYLLT*DNFSFNLLLWRVRQDLLILFCKSCGSPLFG 521
Query: 628 KFGRKGIIEFLILRIALLCRWWIGLSC*HSSG*R*SMLPFPLTIMVGG 675
FGRKGIIE + +L W L C S G* + P L IM+GG
Sbjct: 522 CFGRKGIIECSKIHCPILRLLWNKLKCTLSYG*NFNRQPSALAIMIGG 665
>BF006686
Length = 325
Score = 41.6 bits (96), Expect = 0.001
Identities = 17/39 (43%), Positives = 24/39 (60%)
Frame = +3
Query: 218 IGGNASRMVFWKPLINRINSRLSSWKSKHLSLGGRLVLL 256
+ G + W+PL+ +N L SW +K LS GGR+VLL
Sbjct: 207 VEGRGEILPMWEPLLEHVNKMLKSWGNKLLSFGGRIVLL 323
>TC82948
Length = 705
Score = 41.2 bits (95), Expect = 0.001
Identities = 27/98 (27%), Positives = 46/98 (46%), Gaps = 8/98 (8%)
Frame = +3
Query: 303 KIIWVDWNSVCRSQEVGGLEVRRIKEFNLALLGKWCWRVLTERDSLWFRVLAVRNRVEGG 362
K++ V W VCR + G L +R + + N AL K CW ++ ++ W+ + NR G
Sbjct: 255 KVVKVSWEKVCRPIKEGSLGIRSLSKLNEALNLKLCWDMMISKEQ-WYAFMR-HNRALLG 428
Query: 363 H------LC--SGGRNESMWWRNIVALHSEGWFQNHVS 392
H LC S +++ V++ WF N ++
Sbjct: 429 HIDFMCSLCFKSFESTHHLFFECSVSVQIWDWFSNLIN 542
>BG584442
Length = 775
Score = 40.0 bits (92), Expect = 0.003
Identities = 26/104 (25%), Positives = 45/104 (43%)
Frame = +1
Query: 254 VLLKSVLSSLPVYALSFFKAPSGIVSSIESILNNFFFWGGWGGGSADHRKIIWVDWNSVC 313
V++K L S+ Y +S F + V IE I+N F W + + + W+ +
Sbjct: 430 VMIKYALQSISSYVMSIFLLLNSQVDEIEKIMNTF----SWVHVGENRKGMHWMS*EKLF 597
Query: 314 RSQEVGGLEVRRIKEFNLALLGKWCWRVLTERDSLWFRVLAVRN 357
+ GG+ FN+ +LGK L R +L+ ++ N
Sbjct: 598 VHKNYGGMGFTDFTTFNIPMLGKQV*SFLLNRTTLFLEKIS*HN 729
>TC82763
Length = 1376
Score = 36.2 bits (82), Expect = 0.042
Identities = 23/73 (31%), Positives = 38/73 (51%)
Frame = +1
Query: 309 WNSVCRSQEVGGLEVRRIKEFNLALLGKWCWRVLTERDSLWFRVLAVRNRVEGGHLCSGG 368
WN +CR GG+ +R ++ FN+A + W + TE +S F VL + G L
Sbjct: 244 WNWICRPIR-GGIRIRNLRFFNIA-VR**SWTLKTETNSFHFMVLFHK---YGSWLEGVN 408
Query: 369 RNESMWWRNIVAL 381
+ S+WWR + ++
Sbjct: 409 CSSSLWWRYLSSI 447
>AJ497561 weakly similar to GP|10140689|gb putative non-LTR retroelement
reverse transcriptase {Oryza sativa (japonica
cultivar-group)}, partial (2%)
Length = 621
Score = 34.7 bits (78), Expect = 0.12
Identities = 14/37 (37%), Positives = 29/37 (77%)
Frame = +3
Query: 308 DWNSVCRSQEVGGLEVRRIKEFNLALLGKWCWRVLTE 344
++N++ R + VGGL ++ +EFN+++LGK W++L++
Sbjct: 300 EFNNLARHKSVGGLGLQN-QEFNISMLGKQRWKLLSQ 407
>BF650593
Length = 486
Score = 29.3 bits (64), Expect(2) = 0.84
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = +1
Query: 348 LWFRVLAVRNRVEGGHLCSGGRNESMWWRNI 378
LWFR L + + G + R S+WW++I
Sbjct: 88 LWFRALVNKYGLNRGSITIENRGVSLWWKDI 180
Score = 21.2 bits (43), Expect(2) = 0.84
Identities = 11/24 (45%), Positives = 13/24 (53%), Gaps = 4/24 (16%)
Frame = +2
Query: 304 IIWVDWNSVCRSQEVG----GLEV 323
I+ V W VCR E G GLE+
Sbjct: 32 ILGVKWEKVCRPLEEG*LVCGLEL 103
>AI737603
Length = 531
Score = 31.6 bits (70), Expect = 1.0
Identities = 15/39 (38%), Positives = 22/39 (55%), Gaps = 6/39 (15%)
Frame = -2
Query: 368 GRNESMWWRNIVALHSE------GWFQNHVSRSLGDGSS 400
GR +S WR + A+ S GWF+ ++ R +G GSS
Sbjct: 125 GRRDSSVWRMLSAIRSGVGSGEGGWFEENIRRMVGGGSS 9
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.340 0.151 0.516
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,648,138
Number of Sequences: 36976
Number of extensions: 407838
Number of successful extensions: 3324
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 2437
Number of HSP's successfully gapped in prelim test: 101
Number of HSP's that attempted gapping in prelim test: 797
Number of HSP's gapped (non-prelim): 2643
length of query: 679
length of database: 9,014,727
effective HSP length: 103
effective length of query: 576
effective length of database: 5,206,199
effective search space: 2998770624
effective search space used: 2998770624
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.9 bits)
S2: 62 (28.5 bits)
Medicago: description of AC124215.6