BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124214.6 + phase: 0
(484 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC80709 similar to PIR|B84616|B84616 hypothetical protein At2g22... 328 4e-90
TC82001 similar to GP|14334566|gb|AAK59462.1 unknown protein {Ar... 179 3e-76
TC93582 similar to PIR|B84616|B84616 hypothetical protein At2g22... 108 5e-24
AL385707 homologue to GP|16755592|gb| small GTPase Rab2 {Nicotia... 41 0.001
TC85743 phosphate transporter PT4 [Medicago truncatula] 36 0.029
TC86049 weakly similar to GP|20466248|gb|AAM20441.1 putative tra... 36 0.038
TC77077 similar to GP|9293909|dbj|BAB01812.1 sugar transporter p... 35 0.064
TC86048 weakly similar to PIR|H96561|H96561 probable peptide tra... 34 0.11
TC89164 weakly similar to GP|10177340|dbj|BAB10596. transporter-... 33 0.32
BG587253 homologue to GP|9944648|gb|AA AMV125 {Amsacta moorei en... 32 0.54
AA660656 weakly similar to GP|6498428|dbj| Similar to Arabidopsi... 31 0.93
BG450637 similar to SP|P14895|ELI5_ High molecular mass early li... 31 1.2
TC80103 weakly similar to PIR|H96561|H96561 probable peptide tra... 31 1.2
BQ751186 weakly similar to PIR|T40402|T404 forkhead nuclear sign... 31 1.2
TC81307 weakly similar to GP|4335772|gb|AAD17449.1| unknown prot... 30 1.6
TC93119 similar to GP|6729039|gb|AAF27035.1| unknown protein {Ar... 30 2.1
BQ144079 similar to GP|6523547|emb| hydroxyproline-rich glycopro... 30 2.1
BQ138324 weakly similar to GP|12322902|gb| hypothetical protein;... 29 3.5
TC81270 homologue to GP|21536510|gb|AAM60842.1 unknown {Arabidop... 29 3.5
BG644380 SP|P48020|HMD1 3-hydroxy-3-methylglutaryl-coenzyme A re... 29 3.5
>TC80709 similar to PIR|B84616|B84616 hypothetical protein At2g22730
[imported] - Arabidopsis thaliana, partial (40%)
Length = 1250
Score = 328 bits (840), Expect = 4e-90
Identities = 159/236 (67%), Positives = 194/236 (81%), Gaps = 5/236 (2%)
Frame = +3
Query: 243 QLKDQLSLFLKDMKELLSDKVFVVNVLGYIAYNFVIGAYSYWGPKAGYSIYNMTNADMIF 302
+L +Q + FL DM+ELL ++V+V+NVLGYIAYNFVIGAYSYWGPKAGYSIY+M+NAD++F
Sbjct: 120 KLGNQFTRFLNDMQELLHERVYVINVLGYIAYNFVIGAYSYWGPKAGYSIYHMSNADLLF 299
Query: 303 GGITIVCGILGTLAGGLVLDYMTNTLSNAFKLLSLTTLVGGAFCFGAFAFKSMYGFLALF 362
GGITIVCGI GTLAGGL+LD M++T+SNAFK+LS T +G FC AF FK ++GF+ LF
Sbjct: 300 GGITIVCGIFGTLAGGLILDKMSSTISNAFKILSGATFLGAIFCLVAFLFKGLFGFIILF 479
Query: 363 AIGELLVFATQGPVNFVCLHCVKPSLRPLSMAMSTVAIHIFGDVPSAPLVGVVQDHINNW 422
++GELL+FATQ PVN+V L CVKPSLRPLSMA+STV+IHIFGDVPSAPLVGV+QDHIN+W
Sbjct: 480 SVGELLIFATQAPVNYVSLRCVKPSLRPLSMAISTVSIHIFGDVPSAPLVGVLQDHINDW 659
Query: 423 RTTALILTTIFFPAAAIWFIGIFLNSKDKFNEESEHQVSRVEGTTT-----APLLE 473
R T++ LT+IFF AA +WFIG FL S D FN++ E E TTT PLLE
Sbjct: 660 RKTSICLTSIFFLAAGVWFIGTFLKSDDLFNKDDEED----ESTTTLRGVRKPLLE 815
>TC82001 similar to GP|14334566|gb|AAK59462.1 unknown protein {Arabidopsis
thaliana}, partial (36%)
Length = 623
Score = 179 bits (455), Expect(2) = 3e-76
Identities = 90/118 (76%), Positives = 101/118 (85%), Gaps = 1/118 (0%)
Frame = +3
Query: 27 SVEPNMLPKSTMIPATSWFTPKRLLAIFCVINMLNYLDRGAIASNGVNGHRGTCTD-GIC 85
S+ M+PKS P SWFTPKRLL IFC+IN++NY+DRGAIASNGVNG T T+ G+C
Sbjct: 39 SLRAKMVPKSGQPPNPSWFTPKRLLIIFCIINLINYVDRGAIASNGVNGTLETRTESGVC 218
Query: 86 KSGTGIQGDFNLTNFQDGVLSSAFMVGLLIASPIFASLSKSVNPFRLIGVGLSVWTVA 143
+GTGIQGDFNL+NFQDGVLSSAFMVGLLIASPIFASL+KS NPFRLIGVGLSVWT A
Sbjct: 219 TAGTGIQGDFNLSNFQDGVLSSAFMVGLLIASPIFASLAKSHNPFRLIGVGLSVWTFA 392
Score = 124 bits (311), Expect(2) = 3e-76
Identities = 55/73 (75%), Positives = 62/73 (84%)
Frame = +1
Query: 147 CGLSFNFWSITVCRMLVGVGEASFISLAAPFIDDNAPASQKTVWLSIFYMCIPGGYAIGY 206
CG SF+FWSI +CRML+GV EASFISLAAPFIDDNAPA+QKT WL+ FYMCIP G A+GY
Sbjct: 403 CGSSFDFWSIAICRMLIGVSEASFISLAAPFIDDNAPAAQKTAWLATFYMCIPDGTALGY 582
Query: 207 VYGGVVGSHFGWR 219
V GG+V S F WR
Sbjct: 583 VNGGLVESQFNWR 621
>TC93582 similar to PIR|B84616|B84616 hypothetical protein At2g22730
[imported] - Arabidopsis thaliana, partial (4%)
Length = 591
Score = 108 bits (270), Expect = 5e-24
Identities = 52/54 (96%), Positives = 52/54 (96%)
Frame = +3
Query: 431 TIFFPAAAIWFIGIFLNSKDKFNEESEHQVSRVEGTTTAPLLEEKTAEPKSQEC 484
T FFPAA IWFIGIFLNSKDKFNEESEHQVSRVEGTTTAPLLEEKTAEPKSQEC
Sbjct: 3 TRFFPAAVIWFIGIFLNSKDKFNEESEHQVSRVEGTTTAPLLEEKTAEPKSQEC 164
>AL385707 homologue to GP|16755592|gb| small GTPase Rab2 {Nicotiana tabacum},
partial (10%)
Length = 540
Score = 40.8 bits (94), Expect = 0.001
Identities = 19/21 (90%), Positives = 19/21 (90%)
Frame = -3
Query: 310 GILGTLAGGLVLDYMTNTLSN 330
G GTLAGGLVLDYMTNTLSN
Sbjct: 64 GTRGTLAGGLVLDYMTNTLSN 2
>TC85743 phosphate transporter PT4 [Medicago truncatula]
Length = 1850
Score = 36.2 bits (82), Expect = 0.029
Identities = 30/132 (22%), Positives = 58/132 (43%), Gaps = 7/132 (5%)
Frame = +2
Query: 102 DGVLSSAFMVGLLIASPIFASLSKSVNPFRLIGVGLSVWTVATLCCGLSFNFWS----IT 157
+ V++ +VG L +F L + ++ GV L + +C GLSF + IT
Sbjct: 227 NNVVTGVALVGTLSGQLVFGWLGDKLGRKKVYGVTLIIMVACAICSGLSFGSSAKSVMIT 406
Query: 158 VC--RMLVGVGEASFISLAAPFIDDNAPASQKTVWL-SIFYMCIPGGYAIGYVYGGVVGS 214
+C R +G G L+A + + A + ++ ++F M +G ++ G+V
Sbjct: 407 LCFFRFWLGFGIGGDYPLSATIMSEYANKRTRGAFIAAVFAM-----QGVGIIFAGLVSM 571
Query: 215 HFGWRYAFWVEA 226
F + + +A
Sbjct: 572 VFSGIFKAYYQA 607
>TC86049 weakly similar to GP|20466248|gb|AAM20441.1 putative transport
protein {Arabidopsis thaliana}, partial (43%)
Length = 1836
Score = 35.8 bits (81), Expect = 0.038
Identities = 20/49 (40%), Positives = 28/49 (56%), Gaps = 2/49 (4%)
Frame = +2
Query: 205 GYVYGGVVGSHFGWRYAFWVEAVLMLPFAILGFVMKPL--QLKDQLSLF 251
G VY + H GW+ F V A+LML +L F+ PL ++K + SLF
Sbjct: 164 GIVY---IQDHLGWKVGFGVPAILMLISTVLFFLASPLYVKIKQKTSLF 301
>TC77077 similar to GP|9293909|dbj|BAB01812.1 sugar transporter protein
{Arabidopsis thaliana}, partial (85%)
Length = 1752
Score = 35.0 bits (79), Expect = 0.064
Identities = 41/182 (22%), Positives = 72/182 (39%), Gaps = 5/182 (2%)
Frame = +1
Query: 91 IQGDFNLTNFQDGVLSSAFMVGLLIASPIFASLSKSVNPFRLIGVGLSVWTVATLCCGLS 150
I+ D +++ + VL + LI S + S + I +++ V L G S
Sbjct: 193 IKRDLKVSDGKIEVLLGIINIYSLIGSCLAGRTSDWIGRRYTIVFAGAIFFVGALLMGFS 372
Query: 151 FNFWSITVCRMLVGVGEASFISLAAPFIDDNAPASQKTVWLSIFYMCIPGGYAIGYVYG- 209
N+ + R + GVG + +A + + +PAS + S + I G +GY+
Sbjct: 373 PNYNFLMFGRFVAGVGIGYALMIAPVYTAEVSPASSRGFLTSFPEVFINSGILLGYISNY 552
Query: 210 --GVVGSHFGWRYAFWVEAV--LMLPFAILGFVMKPLQLKDQLSLFLKDMKELLSDKVFV 265
+ GWR V A+ ++L +L P L M+ L D + V
Sbjct: 553 AFSKLSLKLGWRMMLGVGAIPSVILAVGVLAMPESPRWLV---------MRGRLGDAIKV 705
Query: 266 VN 267
+N
Sbjct: 706 LN 711
>TC86048 weakly similar to PIR|H96561|H96561 probable peptide transporter
[imported] - Arabidopsis thaliana, partial (47%)
Length = 1364
Score = 34.3 bits (77), Expect = 0.11
Identities = 18/41 (43%), Positives = 22/41 (52%)
Frame = +3
Query: 205 GYVYGGVVGSHFGWRYAFWVEAVLMLPFAILGFVMKPLQLK 245
G VY + H GWR F V A LML +L F+ PL +K
Sbjct: 678 GIVY---IQDHLGWRIGFGVPAALMLISTVLFFLASPLYVK 791
>TC89164 weakly similar to GP|10177340|dbj|BAB10596. transporter-like
protein {Arabidopsis thaliana}, partial (49%)
Length = 1129
Score = 32.7 bits (73), Expect = 0.32
Identities = 19/77 (24%), Positives = 36/77 (46%), Gaps = 6/77 (7%)
Frame = +3
Query: 94 DFNLT------NFQDGVLSSAFMVGLLIASPIFASLSKSVNPFRLIGVGLSVWTVATLCC 147
DFN+ ++ G + S+FM+G + S ++ +S +I +G+ +
Sbjct: 444 DFNVAKEEADISYYAGYVGSSFMLGRCLTSVLWGMVSDRYGRKPVIIMGIIAVVIFNTLF 623
Query: 148 GLSFNFWSITVCRMLVG 164
GLS +W + R L+G
Sbjct: 624 GLSTGYWMAIITRFLLG 674
>BG587253 homologue to GP|9944648|gb|AA AMV125 {Amsacta moorei
entomopoxvirus}, partial (20%)
Length = 606
Score = 32.0 bits (71), Expect = 0.54
Identities = 26/95 (27%), Positives = 43/95 (44%), Gaps = 5/95 (5%)
Frame = -2
Query: 185 SQKTVWLSIFYMCIPGGYAIGYVYGGVVGSHFGWRYAFWVEA--VLMLPFAILGFVMKPL 242
S++ I++ G +G V GG + + GWR+ +W V +L IL FV +
Sbjct: 302 SERNFAFGIYFAGYCVGDLLGPVIGGCLDQYLGWRWIYWFLTIYVSLLLLLILIFVPETH 123
Query: 243 QLKDQLSLFLKDMKELLS---DKVFVVNVLGYIAY 274
+ S L + LLS +K+ + LG + Y
Sbjct: 122 YRRPSSSSELPLSRSLLSRSQNKLNLFKPLGLLLY 18
>AA660656 weakly similar to GP|6498428|dbj| Similar to Arabidopsis thaliana
chromosome II BAC T27A16 sequence; hypothetical protein.
(AC005496), partial (14%)
Length = 634
Score = 31.2 bits (69), Expect = 0.93
Identities = 23/82 (28%), Positives = 41/82 (49%), Gaps = 1/82 (1%)
Frame = +3
Query: 103 GVLSSAFMVGLLIASPIFASLSKSVNPFRLIGVGLSVWTVAT-LCCGLSFNFWSITVCRM 161
G++ S+F G ++ L+K ++ VG+ +W+VAT L L+ + + R+
Sbjct: 330 GLVQSSFFWGYALSQLPGGWLAKIFGGXNVLVVGVLIWSVATALVPFLAGYMPGLILTRI 509
Query: 162 LVGVGEASFISLAAPFIDDNAP 183
LVG+ E+ S A I + P
Sbjct: 510 LVGIXESVSPSAATDLIAXSIP 575
>BG450637 similar to SP|P14895|ELI5_ High molecular mass early
light-inducible protein HV58 chloroplast precursor
(ELIP). [Barley], partial (10%)
Length = 678
Score = 30.8 bits (68), Expect = 1.2
Identities = 18/47 (38%), Positives = 24/47 (50%)
Frame = -1
Query: 406 VPSAPLVGVVQDHINNWRTTALILTTIFFPAAAIWFIGIFLNSKDKF 452
V +A L + I +WR AL+L FP+A W+ G FL D F
Sbjct: 387 VVTASLETSIGTPILSWRA-ALLLADGVFPSAPFWYTGAFLGPWDPF 250
>TC80103 weakly similar to PIR|H96561|H96561 probable peptide transporter
[imported] - Arabidopsis thaliana, partial (23%)
Length = 1236
Score = 30.8 bits (68), Expect = 1.2
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = +3
Query: 212 VGSHFGWRYAFWVEAVLMLPFAILGFVMKPLQLK 245
+ H GW+ F V A LML I F+ PL +K
Sbjct: 855 IQEHLGWKIGFGVPAALMLLSTICFFLASPLYVK 956
>BQ751186 weakly similar to PIR|T40402|T404 forkhead nuclear signaling
protein - fission yeast (Schizosaccharomyces pombe),
partial (19%)
Length = 711
Score = 30.8 bits (68), Expect = 1.2
Identities = 15/47 (31%), Positives = 24/47 (50%)
Frame = +2
Query: 3 QKSEDEPKPATTTSSSSTPNPVPSSVEPNMLPKSTMIPATSWFTPKR 49
Q+S ++T+S+SST ++ +PN P ST +SW R
Sbjct: 41 QRSNSSSSVSSTSSNSSTTTVATNNSQPNGTPASTNSDMSSWSAASR 181
>TC81307 weakly similar to GP|4335772|gb|AAD17449.1| unknown protein
{Arabidopsis thaliana}, partial (31%)
Length = 1171
Score = 30.4 bits (67), Expect = 1.6
Identities = 17/38 (44%), Positives = 24/38 (62%), Gaps = 1/38 (2%)
Frame = +2
Query: 9 PKPATTTSSSS-TPNPVPSSVEPNMLPKSTMIPATSWF 45
P+P++ TSSSS +P+P PSS P+ KS + S F
Sbjct: 518 PQPSSPTSSSSPSPSPSPSSASPSPSLKSFALSPPSSF 631
>TC93119 similar to GP|6729039|gb|AAF27035.1| unknown protein {Arabidopsis
thaliana}, partial (49%)
Length = 721
Score = 30.0 bits (66), Expect = 2.1
Identities = 20/57 (35%), Positives = 29/57 (50%), Gaps = 10/57 (17%)
Frame = +2
Query: 365 GELLVFATQGPVNFVCLHCVK--------PSLRPL--SMAMSTVAIHIFGDVPSAPL 411
GE L+F + +N C HC K PS RPL SM ++T+ +I+ V + L
Sbjct: 338 GEFLMFYGKQFLNVKCFHCTKAWGQKMSNPSFRPLFISMVIATLRDYIWKKVETRTL 508
>BQ144079 similar to GP|6523547|emb| hydroxyproline-rich glycoprotein DZ-HRGP
{Volvox carteri f. nagariensis}, partial (12%)
Length = 1071
Score = 30.0 bits (66), Expect = 2.1
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = +3
Query: 9 PKPATTTSSSSTPNPVPSSVEPNMLP 34
PKP T + + PNP+P + PN P
Sbjct: 570 PKPPTPQCAKTNPNPIPPATPPNPPP 647
>BQ138324 weakly similar to GP|12322902|gb| hypothetical protein; 50785-52656
{Arabidopsis thaliana}, partial (7%)
Length = 619
Score = 29.3 bits (64), Expect = 3.5
Identities = 14/42 (33%), Positives = 20/42 (47%)
Frame = +2
Query: 9 PKPATTTSSSSTPNPVPSSVEPNMLPKSTMIPATSWFTPKRL 50
P T + S S+ NP PSS P+ +P S + P R+
Sbjct: 158 PSSPTHSRSPSSSNPAPSSPSPSPVPSSIPTSSKPGLNPTRI 283
>TC81270 homologue to GP|21536510|gb|AAM60842.1 unknown {Arabidopsis
thaliana}, complete
Length = 645
Score = 29.3 bits (64), Expect = 3.5
Identities = 29/109 (26%), Positives = 46/109 (41%), Gaps = 16/109 (14%)
Frame = +3
Query: 190 WLSIFYMCI-PGGYAIGYVYGGVVGSHFGWRYAFWVEAVLMLPFAILGFVMKPLQLKDQL 248
W F++ PG + +GY+ G V G W V AV + P + QL +L
Sbjct: 180 WPDYFHLAQGPGNHIMGYIMGKVEGQGESWH--GHVTAVTVAP------DYRRQQLAKKL 335
Query: 249 SLFLKDMKELLSDKVFVVNV---------------LGYIAYNFVIGAYS 282
L+D+ + + DK + V++ LGY+ Y V+ YS
Sbjct: 336 MNLLEDISDNI-DKAYFVDLFVRASNAPAIKMYEKLGYVIYRRVLRYYS 479
>BG644380 SP|P48020|HMD1 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1
(EC 1.1.1.34) (HMG- CoA reductase 1) (HMGR1), partial
(36%)
Length = 697
Score = 29.3 bits (64), Expect = 3.5
Identities = 16/29 (55%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Frame = -2
Query: 122 SLSKSVNPFRLIGVGLSVWTVATL--CCG 148
S S S N +IG G SVW ATL CCG
Sbjct: 609 SSSSSDNAGIIIGAGFSVWDKATLAGCCG 523
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.324 0.139 0.432
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,683,675
Number of Sequences: 36976
Number of extensions: 261126
Number of successful extensions: 2387
Number of sequences better than 10.0: 53
Number of HSP's better than 10.0 without gapping: 2288
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2364
length of query: 484
length of database: 9,014,727
effective HSP length: 100
effective length of query: 384
effective length of database: 5,317,127
effective search space: 2041776768
effective search space used: 2041776768
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 60 (27.7 bits)
Medicago: description of AC124214.6