BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122727.11 + phase: 0 /pseudo/partial
(359 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC88774 similar to PIR|T01394|T01394 hypothetical protein T4I9.1... 703 0.0
TC90395 similar to GP|19310761|gb|AAL85111.1 unknown protein {Ar... 211 3e-55
AW287931 weakly similar to PIR|T01062|T01 hypothetical protein Y... 69 4e-12
TC84177 similar to PIR|T22172|T22172 hypothetical protein F44E5.... 43 2e-04
TC91541 weakly similar to GP|15810253|gb|AAL07014.1 unknown prot... 42 4e-04
BQ141929 similar to GP|19310761|gb| unknown protein {Arabidopsis... 34 0.100
TC76790 similar to PIR|T06817|T06817 RNA-binding protein - garde... 31 0.65
TC80120 homologue to GP|11994319|dbj|BAB02278. casein kinase {Ar... 31 0.84
BG644365 31 0.84
TC87643 similar to PIR|C71405|C71405 probable casein kinase I - ... 31 0.84
BF004353 similar to SP|P22335|HSF2_ Heat shock factor protein HS... 29 2.5
TC92883 29 2.5
TC79403 homologue to GP|10190525|emb|CAC09322. unnamed protein p... 29 3.2
BQ752111 similar to PIR|JQ2282|JQ22 negatively phytochrome regul... 28 5.5
TC80228 similar to GP|21592725|gb|AAM64674.1 unknown {Arabidopsi... 28 5.5
>TC88774 similar to PIR|T01394|T01394 hypothetical protein T4I9.13 -
Arabidopsis thaliana, partial (47%)
Length = 1375
Score = 703 bits (1814), Expect = 0.0
Identities = 356/357 (99%), Positives = 357/357 (99%)
Frame = +2
Query: 3 IQWYPILKNMNFSGTITKPNFPFPPTEMPILTFGHSKLTAATLALQLPCNYIRKIRFITK 62
IQWYPILKNMNFSGTITKPNFPFPPTEMPILTFGHSKLTAATLALQLPCNYIRKIRFITK
Sbjct: 68 IQWYPILKNMNFSGTITKPNFPFPPTEMPILTFGHSKLTAATLALQLPCNYIRKIRFITK 247
Query: 63 LQCSVAGRTDSYRTSAADSQGSKRDSGEIQRKRRGASSLYAGYARPSLSEMKKDKATLRK 122
LQCSVAGRTDSYRTSAADSQGSKRDSGEIQRKRRGASSLYAGYARPSLSEMKKDKATLRK
Sbjct: 248 LQCSVAGRTDSYRTSAADSQGSKRDSGEIQRKRRGASSLYAGYARPSLSEMKKDKATLRK 427
Query: 123 VVYEFLRGIGIVPDELDGLELPVTVDVMKERVDFLHSLGLTIEDINNYPLVLGCSVKKNM 182
VVYEFLRGIGIVPDELDGLELPVTVDVMKERVDFLHSLGLTIEDINNYPLVLGCSVKKNM
Sbjct: 428 VVYEFLRGIGIVPDELDGLELPVTVDVMKERVDFLHSLGLTIEDINNYPLVLGCSVKKNM 607
Query: 183 VPVLDYLGKLGVRKSTITQFLRTYPQVLHASVVVDLVPVVKYLQGMDIKPDDIPRVLERY 242
VPVLDYLGKLGVRKSTITQFLRTYPQVLHASVVVDLVPVVKYLQGMDIKPDDIPRVLERY
Sbjct: 608 VPVLDYLGKLGVRKSTITQFLRTYPQVLHASVVVDLVPVVKYLQGMDIKPDDIPRVLERY 787
Query: 243 PEVLGFKLEGTMSTSVAYLIGIGVGRRELGGILTRFPEILGMRVGRVIKPFVEYLESLGI 302
PEVLGFKLEGTMSTSVAYLIGIGVGRRELGGILT+FPEILGMRVGRVIKPFVEYLESLGI
Sbjct: 788 PEVLGFKLEGTMSTSVAYLIGIGVGRRELGGILTKFPEILGMRVGRVIKPFVEYLESLGI 967
Query: 303 PRLAIARLIETQPYILGFDLDEKVKPNVKSLEEFNVRETSLASIIAQYPDIIGTDLE 359
PRLAIARLIETQPYILGFDLDEKVKPNVKSLEEFNVRETSLASIIAQYPDIIGTDLE
Sbjct: 968 PRLAIARLIETQPYILGFDLDEKVKPNVKSLEEFNVRETSLASIIAQYPDIIGTDLE 1138
>TC90395 similar to GP|19310761|gb|AAL85111.1 unknown protein {Arabidopsis
thaliana}, partial (36%)
Length = 785
Score = 211 bits (537), Expect = 3e-55
Identities = 95/169 (56%), Positives = 138/169 (81%), Gaps = 4/169 (2%)
Frame = +1
Query: 100 SLYAGYARPSLS----EMKKDKATLRKVVYEFLRGIGIVPDELDGLELPVTVDVMKERVD 155
S + Y P+++ + +K+K R +++++L+G+GI+PDEL LELP TV+VM+ERV+
Sbjct: 232 SKFPEYEMPTVTWGVIQGRKEKLVSRVIIFDYLKGLGIIPDELQDLELPSTVEVMRERVE 411
Query: 156 FLHSLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVRKSTITQFLRTYPQVLHASVV 215
F+ LGLTI+DIN YPL+LGCSV+KNM+P+L YL K+G+ +S + +F++ YPQVLHASV+
Sbjct: 412 FIQKLGLTIDDINQYPLILGCSVRKNMIPILGYLEKIGISRSKLGEFIKNYPQVLHASVI 591
Query: 216 VDLVPVVKYLQGMDIKPDDIPRVLERYPEVLGFKLEGTMSTSVAYLIGI 264
V+L PV+K+L+G+D++ DDI VL++YPE+LGFKLEGTMSTSVAYL+ I
Sbjct: 592 VELAPVIKFLRGLDVEKDDIGFVLQKYPELLGFKLEGTMSTSVAYLVSI 738
Score = 56.6 bits (135), Expect = 1e-08
Identities = 40/152 (26%), Positives = 74/152 (48%), Gaps = 1/152 (0%)
Frame = +1
Query: 183 VPVLDYLGKLGVRKSTITQFLRTYPQVLHASVVVDLVPV-VKYLQGMDIKPDDIPRVLER 241
V + DYL LG+ + Q L V+++ V+++Q + + DDI +
Sbjct: 310 VIIFDYLKGLGIIPDEL--------QDLELPSTVEVMRERVEFIQKLGLTIDDI----NQ 453
Query: 242 YPEVLGFKLEGTMSTSVAYLIGIGVGRRELGGILTRFPEILGMRVGRVIKPFVEYLESLG 301
YP +LG + M + YL IG+ R +LG + +P++L V + P +++L L
Sbjct: 454 YPLILGCSVRKNMIPILGYLEKIGISRSKLGEFIKNYPQVLHASVIVELAPVIKFLRGLD 633
Query: 302 IPRLAIARLIETQPYILGFDLDEKVKPNVKSL 333
+ + I +++ P +LGF L+ + +V L
Sbjct: 634 VEKDDIGFVLQKYPELLGFKLEGTMSTSVAYL 729
Score = 55.5 bits (132), Expect = 3e-08
Identities = 36/145 (24%), Positives = 72/145 (48%)
Frame = +1
Query: 215 VVDLVPVVKYLQGMDIKPDDIPRVLERYPEVLGFKLEGTMSTSVAYLIGIGVGRRELGGI 274
+V V + YL+G+ I PD++ + P + M V ++ +G+ ++
Sbjct: 298 LVSRVIIFDYLKGLGIIPDELQDL--ELPSTVE-----VMRERVEFIQKLGLTIDDIN-- 450
Query: 275 LTRFPEILGMRVGRVIKPFVEYLESLGIPRLAIARLIETQPYILGFDLDEKVKPNVKSLE 334
++P ILG V + + P + YLE +GI R + I+ P +L + ++ P +K L
Sbjct: 451 --QYPLILGCSVRKNMIPILGYLEKIGISRSKLGEFIKNYPQVLHASVIVELAPVIKFLR 624
Query: 335 EFNVRETSLASIIAQYPDIIGTDLE 359
+V + + ++ +YP+++G LE
Sbjct: 625 GLDVEKDDIGFVLQKYPELLGFKLE 699
>AW287931 weakly similar to PIR|T01062|T01 hypothetical protein YUP8H12R.46 -
Arabidopsis thaliana, partial (20%)
Length = 503
Score = 68.6 bits (166), Expect = 4e-12
Identities = 39/156 (25%), Positives = 77/156 (49%)
Frame = +2
Query: 167 INNYPLVLGCSVKKNMVPVLDYLGKLGVRKSTITQFLRTYPQVLHASVVVDLVPVVKYLQ 226
I P +LGCS K + V D +LGV++ + Q + PQ+L D + VV + +
Sbjct: 41 IKRQPQLLGCSTSKLKLMV-DQFAELGVQRKKLYQVITRSPQLL-LQKPEDFLQVVMFFE 214
Query: 227 GMDIKPDDIPRVLERYPEVLGFKLEGTMSTSVAYLIGIGVGRRELGGILTRFPEILGMRV 286
M ++I R+L R PE+ + T+ + + +L I V + + ++ ++PE+L +
Sbjct: 215 NMGFDKENIGRILARCPEIFATSISKTLQSKIEFLSRIEVSKAYIPLVIRKYPELLVSDI 394
Query: 287 GRVIKPFVEYLESLGIPRLAIARLIETQPYILGFDL 322
+ + + Y +G+ IA ++ +LG+ +
Sbjct: 395 NKTLPQRIVYFMKVGLSEKEIALMVRKFSPLLGYSI 502
>TC84177 similar to PIR|T22172|T22172 hypothetical protein F44E5.2 -
Caenorhabditis elegans, partial (2%)
Length = 824
Score = 42.7 bits (99), Expect = 2e-04
Identities = 38/169 (22%), Positives = 65/169 (37%)
Frame = +2
Query: 167 INNYPLVLGCSVKKNMVPVLDYLGKLGVRKSTITQFLRTYPQVLHASVVVDLVPVVKYLQ 226
I P +L C+ K ++P +L G S I +R P+ L S+ + +L
Sbjct: 116 IKRVPWILSCNTHKTILPKFQFLLSKGASTSDIVHMVRGNPRFLELSLKNHQIKFEFFLS 295
Query: 227 GMDIKPDDIPRVLERYPEVLGFKLEGTMSTSVAYLIGIGVGRRELGGILTRFPEILGMRV 286
I +L YP++L E + L ++ L + + +
Sbjct: 296 -KGASSSHIVSLLTTYPQILQTSFENRIIPLFKLLTRFFKTNKDTIVCLIQHSKWVTSHP 472
Query: 287 GRVIKPFVEYLESLGIPRLAIARLIETQPYILGFDLDEKVKPNVKSLEE 335
+I + + G+ IARL++ +P I G K +KSLEE
Sbjct: 473 HHLIVASINLISDFGVSDSVIARLLQNKPSIFG------SKDLIKSLEE 601
>TC91541 weakly similar to GP|15810253|gb|AAL07014.1 unknown protein
{Arabidopsis thaliana}, partial (66%)
Length = 820
Score = 42.0 bits (97), Expect = 4e-04
Identities = 19/67 (28%), Positives = 45/67 (66%), Gaps = 1/67 (1%)
Frame = +3
Query: 294 VEYLESLGIPRLAIARLIETQPYILGFDLDEKVKPNVKSL-EEFNVRETSLASIIAQYPD 352
++YL SL + +++L++ P +LG +L E++K N+K L E++++ SL +++ + P
Sbjct: 582 LDYLRSLNLSDDDLSKLLKKFPEVLGCNLKEELKANIKILKEQWSIEGKSLKNLLLRNPK 761
Query: 353 IIGTDLE 359
++G +++
Sbjct: 762 VLGYNID 782
Score = 38.9 bits (89), Expect = 0.003
Identities = 18/64 (28%), Positives = 38/64 (59%), Gaps = 1/64 (1%)
Frame = +3
Query: 224 YLQGMDIKPDDIPRVLERYPEVLGFKLEGTMSTSVAYL-IGIGVGRRELGGILTRFPEIL 282
YL+ +++ DD+ ++L+++PEVLG L+ + ++ L + + L +L R P++L
Sbjct: 588 YLRSLNLSDDDLSKLLKKFPEVLGCNLKEELKANIKILKEQWSIEGKSLKNLLLRNPKVL 767
Query: 283 GMRV 286
G +
Sbjct: 768 GYNI 779
Score = 36.6 bits (83), Expect = 0.015
Identities = 23/87 (26%), Positives = 47/87 (53%), Gaps = 1/87 (1%)
Frame = +3
Query: 240 ERYPEVLGFKLEGTMSTSVAYLIGIGVGRRELGGILTRFPEILGMRVGRVIKPFVEYL-E 298
+R EV F+ +++ ++ YL + + +L +L +FPE+LG + +K ++ L E
Sbjct: 537 DREKEVPKFE---SVNGTLDYLRSLNLSDDDLSKLLKKFPEVLGCNLKEELKANIKILKE 707
Query: 299 SLGIPRLAIARLIETQPYILGFDLDEK 325
I ++ L+ P +LG+++D K
Sbjct: 708 QWSIEGKSLKNLLLRNPKVLGYNIDWK 788
Score = 35.8 bits (81), Expect = 0.026
Identities = 19/67 (28%), Positives = 38/67 (56%), Gaps = 1/67 (1%)
Frame = +3
Query: 186 LDYLGKLGVRKSTITQFLRTYPQVLHASVVVDLVPVVKYL-QGMDIKPDDIPRVLERYPE 244
LDYL L + +++ L+ +P+VL ++ +L +K L + I+ + +L R P+
Sbjct: 582 LDYLRSLNLSDDDLSKLLKKFPEVLGCNLKEELKANIKILKEQWSIEGKSLKNLLLRNPK 761
Query: 245 VLGFKLE 251
VLG+ ++
Sbjct: 762 VLGYNID 782
>BQ141929 similar to GP|19310761|gb| unknown protein {Arabidopsis thaliana},
partial (10%)
Length = 866
Score = 33.9 bits (76), Expect = 0.100
Identities = 13/30 (43%), Positives = 22/30 (73%)
Frame = +3
Query: 114 KKDKATLRKVVYEFLRGIGIVPDELDGLEL 143
+K+K R +++ +L+G GI+PDE + LEL
Sbjct: 129 RKEKLVTRVIIFNYLKG*GIIPDESNELEL 218
>TC76790 similar to PIR|T06817|T06817 RNA-binding protein - garden pea,
partial (79%)
Length = 1141
Score = 31.2 bits (69), Expect = 0.65
Identities = 15/36 (41%), Positives = 23/36 (63%)
Frame = +2
Query: 21 PNFPFPPTEMPILTFGHSKLTAATLALQLPCNYIRK 56
P+FPFPP P ++ SK TA + +L LP + ++K
Sbjct: 125 PSFPFPPN--PSISISPSKPTAQSQSLPLPYSLLKK 226
>TC80120 homologue to GP|11994319|dbj|BAB02278. casein kinase {Arabidopsis
thaliana}, partial (71%)
Length = 1584
Score = 30.8 bits (68), Expect = 0.84
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +1
Query: 148 DVMKERVDFLHSLGLTIEDINNYPLVLGCSVKKNMVPVLDY 188
D + RV+++HS G DI ++G K N V ++DY
Sbjct: 529 DQLINRVEYMHSRGFLHRDIKPDNFLMGLGRKANQVYIIDY 651
>BG644365
Length = 555
Score = 30.8 bits (68), Expect = 0.84
Identities = 15/58 (25%), Positives = 27/58 (45%)
Frame = +3
Query: 290 IKPFVEYLESLGIPRLAIARLIETQPYILGFDLDEKVKPNVKSLEEFNVRETSLASII 347
+ F+E+ ++ G +LI +P +L +D+ +KP K L+ E II
Sbjct: 162 VSAFIEFFQNHGFSATQTKKLIRLRPKLLVSKVDKTLKPKFKFLQSIGFTEDERNKII 335
>TC87643 similar to PIR|C71405|C71405 probable casein kinase I - Arabidopsis
thaliana, partial (71%)
Length = 2349
Score = 30.8 bits (68), Expect = 0.84
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +3
Query: 148 DVMKERVDFLHSLGLTIEDINNYPLVLGCSVKKNMVPVLDY 188
D + RV+++HS G DI ++G K N V ++DY
Sbjct: 900 DQLINRVEYMHSRGFLHRDIKPDNFLMGLGRKANQVYIIDY 1022
>BF004353 similar to SP|P22335|HSF2_ Heat shock factor protein HSF24 (Heat
shock transcription factor 24) (HSTF 24), partial (17%)
Length = 406
Score = 29.3 bits (64), Expect = 2.5
Identities = 13/50 (26%), Positives = 23/50 (46%)
Frame = -3
Query: 173 VLGCSVKKNMVPVLDYLGKLGVRKSTITQFLRTYPQVLHASVVVDLVPVV 222
VL C +K + L YLGK+ S + +P H ++++ P +
Sbjct: 230 VLSCRIKLEKLLCLKYLGKVSSENSAGDHTTKPFPSSFHDTILMPFSPSI 81
>TC92883
Length = 924
Score = 29.3 bits (64), Expect = 2.5
Identities = 17/49 (34%), Positives = 25/49 (50%), Gaps = 3/49 (6%)
Frame = +3
Query: 180 KNMVPVLDYLGKLGVRKSTITQFLRTYPQV---LHASVVVDLVPVVKYL 225
K+++P GKL V K + R V LH + ++D V VVK+L
Sbjct: 585 KSLIPFAIRTGKLAVLKLLVANGCRINDSVDFVLHEAAIIDRVDVVKFL 731
>TC79403 homologue to GP|10190525|emb|CAC09322. unnamed protein product
{Pisum sativum}, partial (42%)
Length = 1027
Score = 28.9 bits (63), Expect = 3.2
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = -3
Query: 6 YPILKNMNFSGTITKPNFPF 25
YP L N NFS TI+ NF +
Sbjct: 608 YPFLSNQNFSNTISSSNFDY 549
>BQ752111 similar to PIR|JQ2282|JQ22 negatively phytochrome regulated protein
I - swollen duckweed, partial (17%)
Length = 737
Score = 28.1 bits (61), Expect = 5.5
Identities = 16/43 (37%), Positives = 27/43 (62%)
Frame = +1
Query: 227 GMDIKPDDIPRVLERYPEVLGFKLEGTMSTSVAYLIGIGVGRR 269
G++ + DD+ +VLE +V+G ++ G VAY+ G G+ RR
Sbjct: 619 GLEARFDDVAKVLEEKNDVVGSRVRG----QVAYVAG-GLPRR 732
>TC80228 similar to GP|21592725|gb|AAM64674.1 unknown {Arabidopsis
thaliana}, partial (42%)
Length = 798
Score = 28.1 bits (61), Expect = 5.5
Identities = 23/99 (23%), Positives = 41/99 (41%)
Frame = +1
Query: 105 YARPSLSEMKKDKATLRKVVYEFLRGIGIVPDELDGLELPVTVDVMKERVDFLHSLGLTI 164
+ R S+ + K+ +KVV +F+ GI + E VDV ++ ++ + +G+ +
Sbjct: 307 HRRDSVDDEPKEHKETKKVVSDFVDGIAKEQQQQKQKENGEEVDVNEDEIEMMKMMGIPV 486
Query: 165 EDINNYPLVLGCSVKKNMVPVLDYLGKLGVRKSTITQFL 203
S K VP D G V K Q++
Sbjct: 487 G---------FDSTKGKPVPGADVSGVRAVTKRQPRQYM 576
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.321 0.141 0.405
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,340,954
Number of Sequences: 36976
Number of extensions: 116496
Number of successful extensions: 628
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 607
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 618
length of query: 359
length of database: 9,014,727
effective HSP length: 97
effective length of query: 262
effective length of database: 5,428,055
effective search space: 1422150410
effective search space used: 1422150410
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
Medicago: description of AC122727.11