Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC122727.10 - phase: 1 /pseudo
         (136 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

AL375901 similar to GP|9759134|dbj mutator-like transposase-like...    47  2e-06
TC79804 weakly similar to GP|6175165|gb|AAF04891.1| Mutator-like...    38  0.001
TC86954 similar to GP|11385433|gb|AAG34799.1 glutathione S-trans...    29  0.50
TC86762 similar to SP|P54967|BIOB_ARATH Biotin synthase (EC 2.8....    28  0.86
TC81520 similar to PIR|D86470|D86470 hypothetical protein AAD460...    27  3.3
TC92357 similar to GP|20259103|gb|AAM14267.1 unknown protein {Ar...    26  4.3
CA917564                                                               26  5.6
TC88417 similar to SP|Q40285|UFO2_MANES Flavonol 3-O-glucosyltra...    25  7.3
TC87218 similar to GP|8778643|gb|AAF79651.1| F5O11.25 {Arabidops...    25  7.3
CA920463 weakly similar to GP|2852684|gb|A resistance protein ca...    25  7.3
TC86955 similar to GP|11385433|gb|AAG34799.1 glutathione S-trans...    25  7.3
BG450473 similar to SP|P51567|AFC2 Protein kinase AFC2 (EC 2.7.1...    25  9.5
BE205299 similar to SP|P51567|AFC2 Protein kinase AFC2 (EC 2.7.1...    25  9.5
AW687663 homologue to GP|13487717|gb sulfate transporter 2 {Lyco...    25  9.5
TC76449 similar to GP|9293996|dbj|BAB01899.1 gene_id:MFD22.1~unk...    25  9.5
TC82742 similar to SP|P51567|AFC2_ARATH Protein kinase AFC2 (EC ...    25  9.5

>AL375901 similar to GP|9759134|dbj mutator-like transposase-like protein
           {Arabidopsis thaliana}, partial (27%)
          Length = 493

 Score = 47.4 bits (111), Expect = 2e-06
 Identities = 24/76 (31%), Positives = 36/76 (46%)
 Frame = +3

Query: 40  NFLRTFKNTEMKKKVRNMGYSMTQPSITHYRNQIRDWSDDVVKWVDDNPKKQWLQAHDQG 99
           NF  TFKNT++     N  Y++T         ++ + S DV+ W    P   W  A+  G
Sbjct: 54  NFRDTFKNTKLVNIFWNAVYALTAAEFESKITEMIEVSQDVISWFQHFPPFLWAVAYFDG 233

Query: 100 RRWGHITTNISECFNN 115
            R+GH T  ++E   N
Sbjct: 234 VRYGHFTLGVTELLYN 281


>TC79804 weakly similar to GP|6175165|gb|AAF04891.1| Mutator-like transposase
            {Arabidopsis thaliana}, partial (57%)
          Length = 2129

 Score = 38.1 bits (87), Expect = 0.001
 Identities = 18/72 (25%), Positives = 35/72 (48%)
 Frame = +1

Query: 40   NFLRTFKNTEMKKKVRNMGYSMTQPSITHYRNQIRDWSDDVVKWVDDNPKKQWLQAHDQG 99
            N  + FKN+ +   + +  Y+ T  +      +I + S +   W+      QW   + +G
Sbjct: 1051 NIGKEFKNSRLIHLLWSAAYATTINAFREKMAEIEEVSPNASMWLQHFHPSQWALVYFEG 1230

Query: 100  RRWGHITTNISE 111
             R+GH+++NI E
Sbjct: 1231 TRYGHLSSNIEE 1266


>TC86954 similar to GP|11385433|gb|AAG34799.1 glutathione S-transferase GST
           9 {Glycine max}, partial (98%)
          Length = 664

 Score = 29.3 bits (64), Expect = 0.50
 Identities = 15/41 (36%), Positives = 23/41 (55%), Gaps = 3/41 (7%)
 Frame = +3

Query: 64  PSITHYRNQIRDWSDDVVKWVDDNPKKQWL---QAHDQGRR 101
           PS  + R Q R W D + K V D  +K WL   + H++G++
Sbjct: 309 PSDPYERAQARFWVDYIDKKVYDTWRKMWLSKGEEHEEGKK 431


>TC86762 similar to SP|P54967|BIOB_ARATH Biotin synthase (EC 2.8.1.6)
           (Biotin synthetase). [Mouse-ear cress] {Arabidopsis
           thaliana}, partial (87%)
          Length = 1555

 Score = 28.5 bits (62), Expect = 0.86
 Identities = 17/56 (30%), Positives = 25/56 (44%)
 Frame = +1

Query: 75  DWSDDVVKWVDDNPKKQWLQAHDQGRRWGHITTNISECFNNVLKGGPQSSNHTNCP 130
           DW+ D VK + D+P    L    Q  R  H    + +C    +K G  S + + CP
Sbjct: 400 DWTKDEVKSIYDSPILDLLFHGAQVHRHAHNFREVQQCTLLSVKTGGCSEDCSYCP 567


>TC81520 similar to PIR|D86470|D86470 hypothetical protein AAD46010.1
           [imported] - Arabidopsis thaliana, partial (39%)
          Length = 1259

 Score = 26.6 bits (57), Expect = 3.3
 Identities = 15/48 (31%), Positives = 27/48 (56%), Gaps = 5/48 (10%)
 Frame = +2

Query: 17  QSKVLLGV*TVIGTMSSAFDT*L-----NFLRTFKNTEMKKKVRNMGY 59
           + + +L + +++G   SA  T L     N ++ F NT +KKK+  MG+
Sbjct: 275 EEQTILHLHSILGNKWSAIATHLPGRTDNEIKNFWNTHLKKKLIQMGF 418


>TC92357 similar to GP|20259103|gb|AAM14267.1 unknown protein {Arabidopsis
           thaliana}, partial (17%)
          Length = 496

 Score = 26.2 bits (56), Expect = 4.3
 Identities = 12/18 (66%), Positives = 13/18 (71%), Gaps = 2/18 (11%)
 Frame = +2

Query: 120 GPQSSNHT--NCPGNLLS 135
           GP S+NHT   CPG LLS
Sbjct: 440 GPDSNNHTAFPCPGELLS 493


>CA917564 
          Length = 656

 Score = 25.8 bits (55), Expect = 5.6
 Identities = 10/31 (32%), Positives = 17/31 (54%)
 Frame = -3

Query: 102 WGHITTNISECFNNVLKGGPQSSNHTNCPGN 132
           +G +   + +C N +LKG P+  N   C G+
Sbjct: 135 FGALI*TLGDCNNGLLKGSPEIVNECCCRGS 43


>TC88417 similar to SP|Q40285|UFO2_MANES Flavonol 3-O-glucosyltransferase
          2 (EC 2.4.1.91) (UDP-glucose flavonoid
          3-O-glucosyltransferase 2), partial (47%)
          Length = 828

 Score = 25.4 bits (54), Expect = 7.3
 Identities = 7/11 (63%), Positives = 10/11 (90%)
 Frame = +3

Query: 78 DDVVKWVDDNP 88
          DD++KW+DD P
Sbjct: 27 DDIIKWLDDQP 59


>TC87218 similar to GP|8778643|gb|AAF79651.1| F5O11.25 {Arabidopsis
           thaliana}, partial (85%)
          Length = 1557

 Score = 25.4 bits (54), Expect = 7.3
 Identities = 24/74 (32%), Positives = 33/74 (44%), Gaps = 4/74 (5%)
 Frame = +3

Query: 39  LNFLRTFK----NTEMKKKVRNMGYSMTQPSITHYRNQIRDWSDDVVKWVDDNPKKQWLQ 94
           ++FL TF     +T M  K         QP ITH R++ R  +          PKKQW+Q
Sbjct: 75  ISFLLTFSCNGGSTNMDNKKNE------QPKITHIRHK-RPITIRHTTNTTK*PKKQWIQ 233

Query: 95  AHDQGRRWGHITTN 108
            H Q +   + T N
Sbjct: 234 -HQQQQPSNNFTYN 272


>CA920463 weakly similar to GP|2852684|gb|A resistance protein candidate
           {Lactuca sativa}, partial (6%)
          Length = 810

 Score = 25.4 bits (54), Expect = 7.3
 Identities = 11/26 (42%), Positives = 16/26 (61%)
 Frame = -1

Query: 76  WSDDVVKWVDDNPKKQWLQAHDQGRR 101
           WS+D VKW++    K  LQ  D+G +
Sbjct: 726 WSEDRVKWIE---VKNLLQVGDEGSK 658


>TC86955 similar to GP|11385433|gb|AAG34799.1 glutathione S-transferase GST
           9 {Glycine max}, complete
          Length = 1065

 Score = 25.4 bits (54), Expect = 7.3
 Identities = 14/35 (40%), Positives = 17/35 (48%)
 Frame = +1

Query: 64  PSITHYRNQIRDWSDDVVKWVDDNPKKQWLQAHDQ 98
           PS  + R Q R W D   K V D  KK WL   ++
Sbjct: 391 PSDPYERAQARFWVDYSDKKVYDTWKKMWLSKGEE 495


>BG450473 similar to SP|P51567|AFC2 Protein kinase AFC2 (EC 2.7.1.-).
           [Mouse-ear cress] {Arabidopsis thaliana}, partial (28%)
          Length = 648

 Score = 25.0 bits (53), Expect = 9.5
 Identities = 8/21 (38%), Positives = 13/21 (61%)
 Frame = +1

Query: 82  KWVDDNPKKQWLQAHDQGRRW 102
           KWV ++  + W    ++GRRW
Sbjct: 181 KWVKEHLDRFWSAGTEKGRRW 243


>BE205299 similar to SP|P51567|AFC2 Protein kinase AFC2 (EC 2.7.1.-).
           [Mouse-ear cress] {Arabidopsis thaliana}, partial (28%)
          Length = 530

 Score = 25.0 bits (53), Expect = 9.5
 Identities = 8/21 (38%), Positives = 13/21 (61%)
 Frame = +3

Query: 82  KWVDDNPKKQWLQAHDQGRRW 102
           KWV ++  + W    ++GRRW
Sbjct: 309 KWVKEHLDRFWSAGTEKGRRW 371


>AW687663 homologue to GP|13487717|gb sulfate transporter 2 {Lycopersicon
          esculentum}, partial (23%)
          Length = 526

 Score = 25.0 bits (53), Expect = 9.5
 Identities = 12/37 (32%), Positives = 22/37 (59%)
 Frame = +1

Query: 11 FQIDTSQSKVLLGV*TVIGTMSSAFDT*LNFLRTFKN 47
          +Q+D ++  V LG   V+G+M+S +    +F R+  N
Sbjct: 58 YQLDGNKEMVALGAMNVVGSMTSCYVATGSFSRSAVN 168


>TC76449 similar to GP|9293996|dbj|BAB01899.1 gene_id:MFD22.1~unknown protein
            {Arabidopsis thaliana}, partial (24%)
          Length = 1154

 Score = 25.0 bits (53), Expect = 9.5
 Identities = 10/21 (47%), Positives = 14/21 (66%)
 Frame = +1

Query: 52   KKVRNMGYSMTQPSITHYRNQ 72
            +KVRN+GY   Q  + H RN+
Sbjct: 1009 QKVRNIGYQRFQHVLQHQRNK 1071


>TC82742 similar to SP|P51567|AFC2_ARATH Protein kinase AFC2 (EC 2.7.1.-).
           [Mouse-ear cress] {Arabidopsis thaliana}, partial (32%)
          Length = 646

 Score = 25.0 bits (53), Expect = 9.5
 Identities = 8/21 (38%), Positives = 13/21 (61%)
 Frame = +1

Query: 82  KWVDDNPKKQWLQAHDQGRRW 102
           KWV ++  + W    ++GRRW
Sbjct: 427 KWVKEHLDRFWSAGTEKGRRW 489


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.329    0.139    0.467 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,131,559
Number of Sequences: 36976
Number of extensions: 71773
Number of successful extensions: 375
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 375
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 375
length of query: 136
length of database: 9,014,727
effective HSP length: 86
effective length of query: 50
effective length of database: 5,834,791
effective search space: 291739550
effective search space used: 291739550
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 53 (25.0 bits)


Medicago: description of AC122727.10