BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122725.8 - phase: 0 /pseudo
(297 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC78818 similar to SP|Q9M1X0|RRF_ARATH Ribosome recycling factor... 473 e-134
TC83216 similar to GP|6091742|gb|AAF03454.1| putative ribosome r... 65 3e-11
TC84863 similar to GP|23498480|emb|CAD50431. hypothetical protei... 32 0.30
CA990666 PIR|C64809|C6 ybgB protein - Escherichia coli, partial ... 32 0.39
TC80242 weakly similar to GP|17062038|dbj|BAB72177. phytosulfoki... 30 0.86
TC92490 similar to GP|7269704|emb|CAB79652.1 predicted protein {... 30 1.1
TC92436 similar to GP|6862922|gb|AAF30311.1| unknown protein {Ar... 29 2.5
TC86958 similar to GP|12321869|gb|AAG50965.1 integral membrane p... 29 2.5
TC77447 similar to GP|16974501|gb|AAL31160.1 AT3g06720/F3E22_14 ... 28 4.3
TC78839 similar to PIR|T01318|T01318 pectinesterase homolog T14P... 28 5.6
TC85782 homologue to GP|21553758|gb|AAM62851.1 ribosomal protein... 27 9.5
TC91769 similar to GP|10434106|dbj|BAB14132. unnamed protein pro... 27 9.5
TC87688 similar to GP|10177535|dbj|BAB10930. gene_id:K1F13.21~un... 27 9.5
>TC78818 similar to SP|Q9M1X0|RRF_ARATH Ribosome recycling factor
chloroplast precursor (Ribosome releasing factor
chloroplast) (RRF), partial (69%)
Length = 935
Score = 473 bits (1216), Expect = e-134
Identities = 258/290 (88%), Positives = 258/290 (88%)
Frame = +1
Query: 1 MATSFSSTTPLRSIFQNLPIVPKALLSAHSSFTIKRSSTYVTLKLSGSGRHSLSKGLLTL 60
MATSFSSTTPLRSIFQNLPIVPKALLSAHSSFTIKRSSTYVTLKLSGSGRHSLSKGLLTL
Sbjct: 16 MATSFSSTTPLRSIFQNLPIVPKALLSAHSSFTIKRSSTYVTLKLSGSGRHSLSKGLLTL 195
Query: 61 HTRYQNYYSRSACFQF*TTSLVFIVICFF*KIPRKGFVRAATIEEIEAEKASIENDVKSR 120
HTR KGFVRAATIEEIEAEKASIENDVKSR
Sbjct: 196 HTR-------------------------------KGFVRAATIEEIEAEKASIENDVKSR 282
Query: 121 MERTLDNVKTNFSSIRTGRATPSMLDKIEVEYYGSPVSLKTIAQISTPDASSLLVSPYDK 180
MERTLDNVKTNFSSIRTGRATPSMLDKIEVEYYGSPVSLKTIAQISTPDASSLLVSPYDK
Sbjct: 283 MERTLDNVKTNFSSIRTGRATPSMLDKIEVEYYGSPVSLKTIAQISTPDASSLLVSPYDK 462
Query: 181 SSLKAIEKAIVNSDVGMTPNNDGEVIRLSIPQLTSDRRKELTKIVSKQVEEGKVALRNIR 240
SSLKAIEKAIVNSDVGMTPNNDGEVIRLSIPQLTSDRRKELTKIVSKQVEEGKVALRNIR
Sbjct: 463 SSLKAIEKAIVNSDVGMTPNNDGEVIRLSIPQLTSDRRKELTKIVSKQVEEGKVALRNIR 642
Query: 241 RDALKAYEKLEKDKKLSEDNVKDLSSDLQKLTDDYIKKVDALYKQKEKVL 290
RDALKAYEKLEKDKKLSEDNVKDLSSDLQKLTDDYIKKVDALYKQKEK L
Sbjct: 643 RDALKAYEKLEKDKKLSEDNVKDLSSDLQKLTDDYIKKVDALYKQKEKEL 792
>TC83216 similar to GP|6091742|gb|AAF03454.1| putative ribosome recycling
factor {Arabidopsis thaliana}, partial (30%)
Length = 794
Score = 65.1 bits (157), Expect = 3e-11
Identities = 35/86 (40%), Positives = 51/86 (58%)
Frame = +3
Query: 112 SIENDVKSRMERTLDNVKTNFSSIRTGRATPSMLDKIEVEYYGSPVSLKTIAQISTPDAS 171
+I+ S+ME + + S +RTGRA+ MLD I VE G + L IA +S D
Sbjct: 537 NIKASASSQMEAAMTALSVELSKLRTGRASAGMLDHIIVETGGVKMPLSRIALVSVLDPK 716
Query: 172 SLLVSPYDKSSLKAIEKAIVNSDVGM 197
+L V+PYD +LK +E AIV+S +G+
Sbjct: 717 TLSVNPYDPDTLKQLENAIVSSPLGL 794
>TC84863 similar to GP|23498480|emb|CAD50431. hypothetical protein;
hypothetical protein with PP2C domain {Plasmodium
falciparum 3D7}, partial (0%)
Length = 838
Score = 32.0 bits (71), Expect = 0.30
Identities = 19/53 (35%), Positives = 28/53 (51%)
Frame = +1
Query: 4 SFSSTTPLRSIFQNLPIVPKALLSAHSSFTIKRSSTYVTLKLSGSGRHSLSKG 56
SF++T L +I NLP + + L F + S T K +G+GRH L +G
Sbjct: 679 SFNATVKLGNISSNLPRLLNSRLLGKLGFMVPLSGCSKTGK*NGNGRHLLVQG 837
>CA990666 PIR|C64809|C6 ybgB protein - Escherichia coli, partial (19%)
Length = 670
Score = 31.6 bits (70), Expect = 0.39
Identities = 25/64 (39%), Positives = 30/64 (46%), Gaps = 5/64 (7%)
Frame = -1
Query: 30 SSFTIKRSSTYVTLKLSGSGRHSLSKGLLTLHTRYQN---YYSRSACF--QF*TTSLVFI 84
S + SST LS S RH +S LLT R + Y+SRS C *T FI
Sbjct: 346 SRIAVCPSST*YLYSLSCSSRHRISSILLTSRIRDSSVVKYHSRSQCICGVM*TRDTAFI 167
Query: 85 VICF 88
V+ F
Sbjct: 166 VVSF 155
>TC80242 weakly similar to GP|17062038|dbj|BAB72177. phytosulfokine
precursor 3_2 {Arabidopsis thaliana}, partial (45%)
Length = 698
Score = 30.4 bits (67), Expect = 0.86
Identities = 16/42 (38%), Positives = 22/42 (52%)
Frame = +1
Query: 247 YEKLEKDKKLSEDNVKDLSSDLQKLTDDYIKKVDALYKQKEK 288
YE LE DK E+N K +S + + + D +Y QKEK
Sbjct: 163 YEVLEDDKVDIEENCKSISEEDCLMRRTLVAHTDYIYTQKEK 288
>TC92490 similar to GP|7269704|emb|CAB79652.1 predicted protein {Arabidopsis
thaliana}, partial (57%)
Length = 1236
Score = 30.0 bits (66), Expect = 1.1
Identities = 41/197 (20%), Positives = 86/197 (42%), Gaps = 6/197 (3%)
Frame = -1
Query: 106 IEAEKASIENDVKSRMERTLDNVKTNF---SSIRTGRATPSMLDKIEVEYYGSPVSLKTI 162
+ A + E ++ +++ ++ K N + I+ A SM ++ S + +
Sbjct: 1092 VGAARKKYEGQLQKFLKKAFEDYKRNAFMEADIQCSNAIHSMEKRLRAACNASDTRVDNV 913
Query: 163 AQISTPDASSLLVSPYDKSSLKAIE-KAIVNSDVGMTPNNDGEVIRLSIPQLTSDRRKEL 221
A++ L+S Y+KS + K + V + + +G V+ L + ++
Sbjct: 912 AKVL-----DALLSEYEKSIQGPGKWKKLA---VFLQQSFEGPVLDLFKRVIDKVESEKS 757
Query: 222 TKIVSKQVEEGKVALRNIRRDALKAYEKLEKDKKLSEDNVKDLSSDLQKLTDDYIKKVDA 281
+ + +++ E K+ L R +A + EK E K+ +D +D +KLTD+Y+ ++
Sbjct: 756 SLALQRRLNEDKMTLLTKRLEASEG-EKSEYIKRY-----EDAINDKKKLTDEYMNRITE 595
Query: 282 LYKQKE--KVLYISTFF 296
L + Y S FF
Sbjct: 594 LQANRR*LDERYSSIFF 544
>TC92436 similar to GP|6862922|gb|AAF30311.1| unknown protein {Arabidopsis
thaliana}, partial (56%)
Length = 710
Score = 28.9 bits (63), Expect = 2.5
Identities = 20/54 (37%), Positives = 30/54 (55%), Gaps = 3/54 (5%)
Frame = +1
Query: 6 SSTTPLRSIFQNLPIVPKALLSAHSSFTIKRSST---YVTLKLSGSGRHSLSKG 56
SST+ RS+ +LP + +L S+HSS + SS+ YV + GS + KG
Sbjct: 178 SSTSSTRSLSFSLPFISPSLTSSHSSSSPSVSSSVSRYVAVLDRGSVSGKVVKG 339
>TC86958 similar to GP|12321869|gb|AAG50965.1 integral membrane protein
putative; 85705-84183 {Arabidopsis thaliana}, partial
(86%)
Length = 1467
Score = 28.9 bits (63), Expect = 2.5
Identities = 30/112 (26%), Positives = 51/112 (44%), Gaps = 9/112 (8%)
Frame = +2
Query: 4 SFSSTTPL---RSIFQNLPIVPKALLSAHSSFTIKRSSTYVTLKLSGSGRHSLSKGLLTL 60
SF S P+ RSI + PI+PK + + +FTI S + + S G L+K LL+
Sbjct: 20 SFYSNKPIPLHRSIHEQNPIIPKTMKESSKTFTITLISAWYS---SNIGVLLLNKYLLS- 187
Query: 61 HTRYQNYYSRSACFQF*TTSLVFIVICFF*KIP------RKGFVRAATIEEI 106
+ ++ + C + ++ I +F +P R F + AT+ I
Sbjct: 188 NYGFKYPIFLTMCHMTACSLFSYVAIAWFKMVPMQFMRSRLQFFKIATLSFI 343
>TC77447 similar to GP|16974501|gb|AAL31160.1 AT3g06720/F3E22_14 {Arabidopsis
thaliana}, partial (96%)
Length = 2271
Score = 28.1 bits (61), Expect = 4.3
Identities = 19/53 (35%), Positives = 29/53 (53%), Gaps = 1/53 (1%)
Frame = -1
Query: 7 STTPLRSIFQNLPIVPKALLSAHSSFTIKRSSTYVTLKLSGSGRHSLS-KGLL 58
S + + S ++ P V A LSA +F + S + T+ + GSG S S KGL+
Sbjct: 1506 SASSINSAYRLTPSVMPAFLSASPTFKMFSSPSRQTVTILGSGHVSRSHKGLM 1348
>TC78839 similar to PIR|T01318|T01318 pectinesterase homolog T14P8.1 -
Arabidopsis thaliana, partial (3%)
Length = 947
Score = 27.7 bits (60), Expect = 5.6
Identities = 17/51 (33%), Positives = 27/51 (52%), Gaps = 6/51 (11%)
Frame = +2
Query: 4 SFSSTTPLRSIFQNLPIVPKA--LLSAHSSFTIKRS----STYVTLKLSGS 48
+ SSTTP + + +LPI P + + H ++ K S Y+TL L+ S
Sbjct: 503 ALSSTTP*KRLSNHLPIAPDSEFAVEVHKAYLTKERLRIVSIYLTLPLTNS 655
>TC85782 homologue to GP|21553758|gb|AAM62851.1 ribosomal protein S15-like
{Arabidopsis thaliana}, complete
Length = 831
Score = 26.9 bits (58), Expect = 9.5
Identities = 13/42 (30%), Positives = 26/42 (60%)
Frame = -1
Query: 115 NDVKSRMERTLDNVKTNFSSIRTGRATPSMLDKIEVEYYGSP 156
N +++R E++L + + S R R+TP L+ + V ++G+P
Sbjct: 252 NLLRAREEKSLTSSSVDISK-RASRSTPRKLNFLNVLFFGTP 130
>TC91769 similar to GP|10434106|dbj|BAB14132. unnamed protein product {Homo
sapiens}, partial (4%)
Length = 828
Score = 26.9 bits (58), Expect = 9.5
Identities = 21/59 (35%), Positives = 29/59 (48%)
Frame = -3
Query: 230 EEGKVALRNIRRDALKAYEKLEKDKKLSEDNVKDLSSDLQKLTDDYIKKVDALYKQKEK 288
E+GK A N YEKL K+ K + KD +L+K ++ +V L K KEK
Sbjct: 550 EKGKRAQNN--------YEKLLKEVKKTNLAEKDTIGELRKKNNELELEVCELRKLKEK 398
>TC87688 similar to GP|10177535|dbj|BAB10930. gene_id:K1F13.21~unknown
protein {Arabidopsis thaliana}, partial (46%)
Length = 2077
Score = 26.9 bits (58), Expect = 9.5
Identities = 17/52 (32%), Positives = 27/52 (51%)
Frame = -2
Query: 1 MATSFSSTTPLRSIFQNLPIVPKALLSAHSSFTIKRSSTYVTLKLSGSGRHS 52
+++SFS + SIF+NL +P L + SS + SS + S S + S
Sbjct: 630 LSSSFSKYSVNSSIFKNLSSIPPPLTFSFSSSSSSSSSLPSSSSSSSSSKSS 475
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.133 0.359
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,196,402
Number of Sequences: 36976
Number of extensions: 88519
Number of successful extensions: 436
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 435
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 436
length of query: 297
length of database: 9,014,727
effective HSP length: 95
effective length of query: 202
effective length of database: 5,502,007
effective search space: 1111405414
effective search space used: 1111405414
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC122725.8