BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122169.4 - phase: 0
(165 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI263941 homologue to GP|20160684|dbj contains EST C91729(E31432... 334 1e-92
BI269821 similar to GP|20160684|dbj contains EST C91729(E31432)~... 56 6e-09
BM814300 similar to PIR|T47833|T478 hypothetical protein T2O9.70... 37 0.004
AL378289 similar to PIR|T46022|T460 hypothetical protein T10K17.... 35 0.010
TC91290 weakly similar to PIR|G96836|G96836 unknown protein T21F... 32 0.089
BF646120 similar to GP|17064156|gb| WRKY transcription factor 22... 32 0.12
TC84329 similar to PIR|C86480|C86480 hypothetical protein AAG282... 31 0.20
AW689511 similar to GP|14140155|emb putative AP2 domain transcri... 31 0.26
AJ501905 similar to GP|21740554|emb OSJNBa0033G16.4 {Oryza sativ... 30 0.34
TC92312 weakly similar to GP|4567275|gb|AAD23688.1| expressed pr... 30 0.58
TC88566 similar to GP|21536824|gb|AAM61156.1 unknown {Arabidopsi... 29 0.98
TC85085 similar to GP|21618600|gb|AAH32743.1 GCN5 general contro... 28 1.7
TC88047 similar to PIR|T50561|T50561 SINA1 protein [imported] - ... 28 2.2
TC76532 weakly similar to SP|Q9BBN6|YCF1_LOTJA Hypothetical 214.... 28 2.2
BI270652 similar to PIR|A84766|A847 hypothetical protein At2g352... 28 2.2
TC76470 similar to PIR|D84651|D84651 hypothetical protein At2g25... 27 2.9
TC81296 similar to GP|6707571|gb|AAF25671.1| ATP-dependent phosp... 27 3.7
AJ503842 similar to GP|15982737|gb| AT4g23750/F9D16_220 {Arabido... 27 4.9
TC82499 SP|Q9XHM1|IF38_MEDTR Eukaryotic translation initiation f... 27 4.9
TC77154 weakly similar to PIR|S49878|S49878 probable narbonin - ... 27 4.9
>BI263941 homologue to GP|20160684|dbj contains EST C91729(E31432)~unknown
protein {Oryza sativa (japonica cultivar-group)},
partial (16%)
Length = 662
Score = 334 bits (856), Expect = 1e-92
Identities = 165/165 (100%), Positives = 165/165 (100%)
Frame = +3
Query: 1 MDSISTSSTSSSVISTVQQKTPTKLTKPKKKKNNNNTYNKPIKVVYISNPMKVKTSASEF 60
MDSISTSSTSSSVISTVQQKTPTKLTKPKKKKNNNNTYNKPIKVVYISNPMKVKTSASEF
Sbjct: 36 MDSISTSSTSSSVISTVQQKTPTKLTKPKKKKNNNNTYNKPIKVVYISNPMKVKTSASEF 215
Query: 61 RALVQELTGQYAESPPNPSRFQEFDVNDSGTDQGGCENMMDCDKSDQTVVGVPSLVDPDD 120
RALVQELTGQYAESPPNPSRFQEFDVNDSGTDQGGCENMMDCDKSDQTVVGVPSLVDPDD
Sbjct: 216 RALVQELTGQYAESPPNPSRFQEFDVNDSGTDQGGCENMMDCDKSDQTVVGVPSLVDPDD 395
Query: 121 KGKPSEAGSSNESFDEDVLLMPEMMDNIWDLLPTSAFYESFQLDS 165
KGKPSEAGSSNESFDEDVLLMPEMMDNIWDLLPTSAFYESFQLDS
Sbjct: 396 KGKPSEAGSSNESFDEDVLLMPEMMDNIWDLLPTSAFYESFQLDS 530
>BI269821 similar to GP|20160684|dbj contains EST C91729(E31432)~unknown
protein {Oryza sativa (japonica cultivar-group)},
partial (16%)
Length = 687
Score = 56.2 bits (134), Expect = 6e-09
Identities = 28/41 (68%), Positives = 31/41 (75%)
Frame = +3
Query: 30 KKKNNNNTYNKPIKVVYISNPMKVKTSASEFRALVQELTGQ 70
K K + K +KV YIS+PMKVKTSAS FRALVQELTGQ
Sbjct: 114 KGKKQSKKNKKEVKVTYISSPMKVKTSASNFRALVQELTGQ 236
>BM814300 similar to PIR|T47833|T478 hypothetical protein T2O9.70 -
Arabidopsis thaliana, partial (27%)
Length = 626
Score = 37.0 bits (84), Expect = 0.004
Identities = 23/69 (33%), Positives = 35/69 (50%)
Frame = +3
Query: 5 STSSTSSSVISTVQQKTPTKLTKPKKKKNNNNTYNKPIKVVYISNPMKVKTSASEFRALV 64
S++ST+SS + + KPK I++++I P +KT A+ FR LV
Sbjct: 126 SSTSTTSSKLGVDKDSQVISKVKPK------------IRIIHIYAPEIIKTDAANFRELV 269
Query: 65 QELTGQYAE 73
Q LTG+ E
Sbjct: 270 QRLTGKPEE 296
>AL378289 similar to PIR|T46022|T460 hypothetical protein T10K17.210 -
Arabidopsis thaliana, partial (25%)
Length = 476
Score = 35.4 bits (80), Expect = 0.010
Identities = 22/70 (31%), Positives = 34/70 (48%)
Frame = +1
Query: 8 STSSSVISTVQQKTPTKLTKPKKKKNNNNTYNKPIKVVYISNPMKVKTSASEFRALVQEL 67
S S+ K K++K K K I+++++ P +KT A FR LVQ+L
Sbjct: 70 SNSNQPNFLAMHKDSHKISKMKPK----------IRIIHVYAPEIIKTDAENFRELVQKL 219
Query: 68 TGQYAESPPN 77
TG+ +E N
Sbjct: 220 TGKPSEEKKN 249
>TC91290 weakly similar to PIR|G96836|G96836 unknown protein T21F11.18
[imported] - Arabidopsis thaliana, partial (14%)
Length = 980
Score = 32.3 bits (72), Expect = 0.089
Identities = 15/34 (44%), Positives = 24/34 (70%)
Frame = -3
Query: 6 TSSTSSSVISTVQQKTPTKLTKPKKKKNNNNTYN 39
T+S+ + ST + P K+ KPKKKKN+N+T++
Sbjct: 909 TTSSLYFLKSTQTK*DPPKMEKPKKKKNSNDTFH 808
>BF646120 similar to GP|17064156|gb| WRKY transcription factor 22
{Arabidopsis thaliana}, partial (21%)
Length = 615
Score = 32.0 bits (71), Expect = 0.12
Identities = 25/93 (26%), Positives = 37/93 (38%), Gaps = 20/93 (21%)
Frame = +1
Query: 6 TSSTSSSVISTVQQKTPTKLTKPKKKKNNNNTYN--------KPIKVVYISNPMKVKTSA 57
T+ TSSS ST T + NNNN N + +V +SNP + K+S
Sbjct: 100 TNLTSSSSSSTTTSTTTLATSSTHSYNNNNNNNNFFSIYPTEQTSQVFSLSNPFETKSSI 279
Query: 58 SEFRALVQEL------------TGQYAESPPNP 78
E L + +G ++ S P+P
Sbjct: 280 EELHELCKPFFFKSSSSSQPLSSGSFSYSSPSP 378
>TC84329 similar to PIR|C86480|C86480 hypothetical protein AAG28299.1
[imported] - Arabidopsis thaliana, partial (14%)
Length = 730
Score = 31.2 bits (69), Expect = 0.20
Identities = 30/82 (36%), Positives = 37/82 (44%), Gaps = 3/82 (3%)
Frame = +1
Query: 3 SISTSSTSSSVISTVQQKTPTKLTKPKKKKNNNNTYNKPIKVVYISN--PMKVKTS-ASE 59
S+ STS IS LT ++ NNNN P K S P V T+ +
Sbjct: 499 SLPQESTSRGGIS---------LTNDQQNLNNNNMGRNPKKRSRASRRAPTTVLTTDTTN 651
Query: 60 FRALVQELTGQYAESPPNPSRF 81
FRA+VQE TG +PP S F
Sbjct: 652 FRAMVQEFTG--IPAPPFSSPF 711
>AW689511 similar to GP|14140155|emb putative AP2 domain transcription factor
{Oryza sativa}, partial (32%)
Length = 703
Score = 30.8 bits (68), Expect = 0.26
Identities = 13/37 (35%), Positives = 22/37 (59%)
Frame = +1
Query: 5 STSSTSSSVISTVQQKTPTKLTKPKKKKNNNNTYNKP 41
STSS S ++Q+++PT+ +P+ NN+N P
Sbjct: 163 STSSQESPPTHSIQKQSPTRPKRPRDDDNNDNNNKHP 273
>AJ501905 similar to GP|21740554|emb OSJNBa0033G16.4 {Oryza sativa}, partial
(8%)
Length = 269
Score = 30.4 bits (67), Expect = 0.34
Identities = 23/73 (31%), Positives = 33/73 (44%), Gaps = 6/73 (8%)
Frame = +1
Query: 2 DSISTSSTSSSVISTVQQKTPTKLTKPKKK-KNNNNTYNKPIKV-----VYISNPMKVKT 55
++IST + + PT LT K K T I++ +Y +P + T
Sbjct: 49 NNISTERSQKRELQLQGLPRPTPLTINKNSHKIKKPTLGPKIQIHEPVIIYTESPKIIHT 228
Query: 56 SASEFRALVQELT 68
S SEF +LVQ LT
Sbjct: 229 SPSEFMSLVQSLT 267
>TC92312 weakly similar to GP|4567275|gb|AAD23688.1| expressed protein
{Arabidopsis thaliana}, partial (29%)
Length = 1319
Score = 29.6 bits (65), Expect = 0.58
Identities = 24/95 (25%), Positives = 43/95 (45%), Gaps = 7/95 (7%)
Frame = +1
Query: 78 PSRFQEFDVNDSGTDQGGCENMMDCDKSDQTVVGVPSLVDPDDKGKPSEAGS-------S 130
P + D ND+ + G + + D D S T + + D+G S+ S S
Sbjct: 508 PKKIFSSDTNDAPASEEGQQKVTDEDGSTTTEDDLENTDKKSDQGDDSDIDSVVEEDVPS 687
Query: 131 NESFDEDVLLMPEMMDNIWDLLPTSAFYESFQLDS 165
+ FD++ + ++++N L+ +SA ES DS
Sbjct: 688 EDDFDKEADIARKVLNN---LITSSAKDESVNNDS 783
>TC88566 similar to GP|21536824|gb|AAM61156.1 unknown {Arabidopsis
thaliana}, partial (65%)
Length = 1432
Score = 28.9 bits (63), Expect = 0.98
Identities = 15/73 (20%), Positives = 33/73 (44%)
Frame = +1
Query: 7 SSTSSSVISTVQQKTPTKLTKPKKKKNNNNTYNKPIKVVYISNPMKVKTSASEFRALVQE 66
SST +++S++ +TP+ L+ P ++ +N + + P+ S + ++ +
Sbjct: 253 SSTKKTLLSSLNPQTPSSLSNPSPSNHSQQNHNHHHRRRHHRRPLPFNPSRNSLQSFKKL 432
Query: 67 LTGQYAESPPNPS 79
T P PS
Sbjct: 433 *TSSNPCKNPKPS 471
>TC85085 similar to GP|21618600|gb|AAH32743.1 GCN5 general control of
amino-acid synthesis 5-like 2 (yeast) {Homo sapiens},
partial (3%)
Length = 693
Score = 28.1 bits (61), Expect = 1.7
Identities = 21/72 (29%), Positives = 30/72 (41%)
Frame = +3
Query: 7 SSTSSSVISTVQQKTPTKLTKPKKKKNNNNTYNKPIKVVYISNPMKVKTSASEFRALVQE 66
SS+SSS+ S+ QKT + +K NT PI KT R+ +
Sbjct: 282 SSSSSSMPSSPIQKTTQYSSSSSPEKTTANTSRVPIPT------QSPKTIKQNVRSPMHS 443
Query: 67 LTGQYAESPPNP 78
+ SPP+P
Sbjct: 444 PNARSTNSPPSP 479
>TC88047 similar to PIR|T50561|T50561 SINA1 protein [imported] - Vitis
vinifera, partial (50%)
Length = 831
Score = 27.7 bits (60), Expect = 2.2
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = -1
Query: 21 TPTKLTKPKKKKNNNNTYNKPIKVVYISNPMKVK 54
TP+ LTKP K+K N K ++ Y+ P+ +K
Sbjct: 291 TPSTLTKPLKQKQPTNQNRKFHQINYLGIPLFLK 190
>TC76532 weakly similar to SP|Q9BBN6|YCF1_LOTJA Hypothetical 214.8 kDa
protein ycf1. {Lotus japonicus}, partial (21%)
Length = 4509
Score = 27.7 bits (60), Expect = 2.2
Identities = 9/32 (28%), Positives = 20/32 (62%)
Frame = +2
Query: 28 PKKKKNNNNTYNKPIKVVYISNPMKVKTSASE 59
PKKKK N+Y++ ++++ + P ++ + E
Sbjct: 680 PKKKKEKKNSYSRQLELIRTTKPFLIERNVLE 775
>BI270652 similar to PIR|A84766|A847 hypothetical protein At2g35230
[imported] - Arabidopsis thaliana, partial (19%)
Length = 649
Score = 27.7 bits (60), Expect = 2.2
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +2
Query: 50 PMKVKTSASEFRALVQELTGQYAESPP 76
P S ++FR +VQ+LTG ++ PP
Sbjct: 206 PQVYNISKNDFRDIVQQLTGSPSQEPP 286
>TC76470 similar to PIR|D84651|D84651 hypothetical protein At2g25680
[imported] - Arabidopsis thaliana, partial (53%)
Length = 1344
Score = 27.3 bits (59), Expect = 2.9
Identities = 15/28 (53%), Positives = 19/28 (67%), Gaps = 1/28 (3%)
Frame = -3
Query: 23 TKLTKPKKKKNNNNTYNKPI-KVVYISN 49
TK TK KKKKN+N T N+ I K ++ N
Sbjct: 1204 TKKTKFKKKKNSNLTENQHIMKTIHTKN 1121
>TC81296 similar to GP|6707571|gb|AAF25671.1| ATP-dependent
phosphoenolpyruvate carboxykinase {Medicago sativa},
partial (44%)
Length = 813
Score = 26.9 bits (58), Expect = 3.7
Identities = 17/43 (39%), Positives = 24/43 (55%)
Frame = +3
Query: 1 MDSISTSSTSSSVISTVQQKTPTKLTKPKKKKNNNNTYNKPIK 43
MDS SS +V+ +T +L +KKKNN +T N PI+
Sbjct: 117 MDSSEICHDDSS--PSVKAQTIHELHSLQKKKNNKSTPNTPIR 239
>AJ503842 similar to GP|15982737|gb| AT4g23750/F9D16_220 {Arabidopsis
thaliana}, partial (20%)
Length = 541
Score = 26.6 bits (57), Expect = 4.9
Identities = 17/43 (39%), Positives = 23/43 (52%), Gaps = 4/43 (9%)
Frame = +3
Query: 21 TPTKLTKPKKKKNNNNTYN----KPIKVVYISNPMKVKTSASE 59
T K TKPKK NNNN + I+V Y ++P +S+ E
Sbjct: 39 TKMKYTKPKKHTNNNNKKSIQIPSVIRVTY-TDPDATDSSSDE 164
>TC82499 SP|Q9XHM1|IF38_MEDTR Eukaryotic translation initiation factor 3
subunit 8 (eIF3 p110) (eIF3c). [Barrel medic], partial
(91%)
Length = 2816
Score = 26.6 bits (57), Expect = 4.9
Identities = 21/70 (30%), Positives = 32/70 (45%), Gaps = 5/70 (7%)
Frame = +1
Query: 73 ESPPNPSRF-----QEFDVNDSGTDQGGCENMMDCDKSDQTVVGVPSLVDPDDKGKPSEA 127
E P SRF + D +D TD +D + SD P+ DP+ K K
Sbjct: 229 EKPTMTSRFFYQGGDQSDTDDEPTD-------IDDEPSDTE----PAPTDPNGKSKYLAG 375
Query: 128 GSSNESFDED 137
G++++S D+D
Sbjct: 376 GNADDSDDDD 405
>TC77154 weakly similar to PIR|S49878|S49878 probable narbonin - soybean,
partial (32%)
Length = 1260
Score = 26.6 bits (57), Expect = 4.9
Identities = 19/90 (21%), Positives = 42/90 (46%), Gaps = 9/90 (10%)
Frame = +1
Query: 21 TPTKLTKPKKKKNNNNTYNKPIKVVYIS---------NPMKVKTSASEFRALVQELTGQY 71
+P K+ K KK N +KV+ NP ++++ +++ + +++L +Y
Sbjct: 274 SPAKVAKLKKDHKN-------VKVIISIGGFGAENPFNPKEIESWSTKAKQSIKKLINEY 432
Query: 72 AESPPNPSRFQEFDVNDSGTDQGGCENMMD 101
QE+ + S TD+ C++++D
Sbjct: 433 ----------QEYSKDSSSTDECHCDDIID 492
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.306 0.125 0.346
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,417,678
Number of Sequences: 36976
Number of extensions: 53420
Number of successful extensions: 406
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 394
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 400
length of query: 165
length of database: 9,014,727
effective HSP length: 89
effective length of query: 76
effective length of database: 5,723,863
effective search space: 435013588
effective search space used: 435013588
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)
S2: 54 (25.4 bits)
Medicago: description of AC122169.4