BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122166.9 + phase: 0 /pseudo
(249 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC77850 weakly similar to PIR|H96517|H96517 protein T2E6.22 [imp... 262 1e-70
BG646718 weakly similar to PIR|H96517|H96 protein T2E6.22 [impor... 132 4e-38
BF637378 weakly similar to PIR|H96517|H965 protein T2E6.22 [impo... 133 7e-32
BF006197 weakly similar to GP|1310677|emb| protein z-type serpin... 87 5e-18
AW560154 71 3e-13
AW573956 similar to GP|20145851|emb auxin response factor 36 {Ar... 29 1.5
TC76323 homologue to GP|12054507|emb|CAC20221. ribosomal protein... 28 4.3
TC77705 similar to GP|21553857|gb|AAM62950.1 unknown {Arabidopsi... 28 4.3
BM815439 28 4.3
TC82714 similar to PIR|T07142|T07142 beta-glucan-elicitor recept... 27 7.4
TC78253 weakly similar to GP|22654983|gb|AAM98084.1 AT5g45110/K1... 27 7.4
TC84816 similar to GP|2313789|gb|AAD07729.1| H. pylori predicted... 27 7.4
TC90831 similar to GP|12831474|gb|AAB05871.2 PAPS-reductase-like... 27 9.6
BG587125 similar to SP|Q9RQ15|RS16_ 30S ribosomal protein S16. {... 27 9.6
BE319112 similar to GP|3377842|gb| T27D20.12 gene product {Arabi... 27 9.6
TC93048 similar to GP|13516922|dbj|BAB40340. 12-oxophytodienoic ... 27 9.6
>TC77850 weakly similar to PIR|H96517|H96517 protein T2E6.22 [imported] -
Arabidopsis thaliana, partial (65%)
Length = 1485
Score = 262 bits (669), Expect = 1e-70
Identities = 140/248 (56%), Positives = 177/248 (70%), Gaps = 11/248 (4%)
Frame = +2
Query: 6 RKRTKKNLIGKSLTKFTNVSLNITKHLLSNQKLNEKNIVFSPLSLNTVLIMIATGSEGPT 65
R + K + +S+ TNVSL++ KHL S + ++ NIVFSPLSL VL +IA+GSEGPT
Sbjct: 23 RNKQKTMDLRESIANQTNVSLSVAKHLFSKE--SDNNIVFSPLSLQVVLSIIASGSEGPT 196
Query: 66 QNQLLSFLQSESTGDLKSLCSQVVSSVLSDGARAGGPCLSYVNGVWVEKSLPLQPSFKQL 125
Q QL +FLQS+ST L SQ+VS +LSD + AGGP LS+V+GVWV+++L LQPSF+Q+
Sbjct: 197 QQQLFNFLQSKSTDHLNYFASQLVSVILSDASPAGGPLLSFVDGVWVDQTLSLQPSFQQI 376
Query: 126 MTTDFEATLSAVDFVNK-----------AEKKTKGLIQDLLPHGSVNSLTSLIFANALYF 174
++T F+A LS+VDF NK AEK+T GLI++LLP GSVN+ T LIFANALYF
Sbjct: 377 VSTHFKAALSSVDFQNKAVEVTNEVNSWAEKETNGLIKELLPLGSVNNATRLIFANALYF 556
Query: 175 KGVWKQKFDTSKTKDYDFDLLNGKSVKVPFMTSKNDQFISSLDGFKVLGLPINKAKMGVH 234
KG W KFD SKT+DY+F LLNG VKVPFMTSK QFI + DGFKVLGLP + +
Sbjct: 557 KGAWNDKFDASKTEDYEFHLLNGSPVKVPFMTSKKKQFIRAFDGFKVLGLPYKQGE-DKR 733
Query: 235 SLFTFFFL 242
+FFL
Sbjct: 734 QFTMYFFL 757
>BG646718 weakly similar to PIR|H96517|H96 protein T2E6.22 [imported] -
Arabidopsis thaliana, partial (30%)
Length = 702
Score = 132 bits (331), Expect(2) = 4e-38
Identities = 66/107 (61%), Positives = 85/107 (78%), Gaps = 11/107 (10%)
Frame = +2
Query: 90 SSVLSDGARAGGPCLSYVNGVWVEKSLPLQPSFKQLMTTDFEATLSAVDFVNKA------ 143
S VLSD A AGGP LS+V+GVW+EK+L LQPSFKQ+M++D++ATL++VDF+ KA
Sbjct: 224 SIVLSDAASAGGPHLSFVDGVWIEKTLSLQPSFKQIMSSDYKATLASVDFMFKAPEVRKE 403
Query: 144 -----EKKTKGLIQDLLPHGSVNSLTSLIFANALYFKGVWKQKFDTS 185
EK+T GLI++L+P GSV+SLTSLIFANALYF+G W +KFD S
Sbjct: 404 VNIWVEKETNGLIKELIPPGSVDSLTSLIFANALYFRGAWNEKFDVS 544
Score = 43.1 bits (100), Expect(2) = 4e-38
Identities = 24/41 (58%), Positives = 28/41 (67%)
Frame = +1
Query: 17 SLTKFTNVSLNITKHLLSNQKLNEKNIVFSPLSLNTVLIMI 57
S+T T VSL I KHL S Q +E NIVFSPLSL VL ++
Sbjct: 22 SMTNQTRVSLRIAKHLFSKQ--SENNIVFSPLSLQVVLSIL 138
>BF637378 weakly similar to PIR|H96517|H965 protein T2E6.22 [imported] -
Arabidopsis thaliana, partial (9%)
Length = 627
Score = 133 bits (334), Expect = 7e-32
Identities = 72/136 (52%), Positives = 92/136 (66%)
Frame = +1
Query: 22 TNVSLNITKHLLSNQKLNEKNIVFSPLSLNTVLIMIATGSEGPTQNQLLSFLQSESTGDL 81
T VSL I KHL S + +EKNIVFSPLSL VL +I GSEG TQ QLL FL+ +ST L
Sbjct: 37 TKVSLTIVKHLFSKE--SEKNIVFSPLSLQVVLSIITAGSEGSTQQQLLDFLRFKSTDHL 210
Query: 82 KSLCSQVVSSVLSDGARAGGPCLSYVNGVWVEKSLPLQPSFKQLMTTDFEATLSAVDFVN 141
S S +S +L D +GGP LS+V+GVW+EK+L LQ SFKQ+++ D++ATLS VDF N
Sbjct: 211 NSSVSHQLSIILKDATSSGGPHLSFVDGVWLEKTLSLQSSFKQIVSEDYKATLSEVDFKN 390
Query: 142 KAEKKTKGLIQDLLPH 157
K +++ L H
Sbjct: 391 KIHGTEIQILEGALKH 438
>BF006197 weakly similar to GP|1310677|emb| protein z-type serpin {Hordeum
vulgare subsp. vulgare}, partial (7%)
Length = 468
Score = 87.4 bits (215), Expect = 5e-18
Identities = 44/87 (50%), Positives = 59/87 (67%)
Frame = +3
Query: 56 MIATGSEGPTQNQLLSFLQSESTGDLKSLCSQVVSSVLSDGARAGGPCLSYVNGVWVEKS 115
++ EG TQ QLL FL+S+ L S S +VS +LSD A +GG LS VWV+++
Sbjct: 3 IVTAACEGRTQQQLLEFLRSKFIDHLNSFTSHLVSIILSDAAPSGGSRLSLTQRVWVDQT 182
Query: 116 LPLQPSFKQLMTTDFEATLSAVDFVNK 142
L LQPSFK+ M TD++ATL++VDF NK
Sbjct: 183 LSLQPSFKETMVTDYKATLASVDFQNK 263
>AW560154
Length = 470
Score = 71.2 bits (173), Expect = 3e-13
Identities = 34/62 (54%), Positives = 46/62 (73%)
Frame = +1
Query: 81 LKSLCSQVVSSVLSDGARAGGPCLSYVNGVWVEKSLPLQPSFKQLMTTDFEATLSAVDFV 140
L S S +VS +LSD A +GG LS+ VWV+++L LQPSFK+ M TD++ATL++VDF
Sbjct: 7 LNSFTSDLVSIILSDAAPSGGSRLSFTQRVWVDQTLSLQPSFKETMVTDYKATLASVDFQ 186
Query: 141 NK 142
NK
Sbjct: 187 NK 192
>AW573956 similar to GP|20145851|emb auxin response factor 36 {Arabidopsis
thaliana}, partial (15%)
Length = 555
Score = 29.3 bits (64), Expect = 1.5
Identities = 23/79 (29%), Positives = 37/79 (46%), Gaps = 3/79 (3%)
Frame = +2
Query: 125 LMTTDFEATLSAVD-FVNKAEKKTKGLIQDLLPHGSVNSLTSLIFANALYFKGVWKQKFD 183
L TTDF+ L+ F + +KK + P G+V + N +YF G W++
Sbjct: 176 LQTTDFQQQLALPKTFSDNLKKKLPENVTLKGPSGAVWDIGLTTRDNTVYFVGGWERFVK 355
Query: 184 TSKTKDYDFDLL--NGKSV 200
K+ DF + NG+S+
Sbjct: 356 DHSLKENDFLVFKYNGESL 412
>TC76323 homologue to GP|12054507|emb|CAC20221. ribosomal protein L2
{Glycine max}, complete
Length = 1031
Score = 27.7 bits (60), Expect = 4.3
Identities = 25/82 (30%), Positives = 39/82 (47%), Gaps = 5/82 (6%)
Frame = -1
Query: 19 TKFTNVSLNITKHLLSNQKLNEKNIVFSPLSLNTVLIMIATGSEGPTQNQ-LLSFL---- 73
T TN++LNIT H + +NI + L T + + TG + + N+ LL FL
Sbjct: 419 TTVTNMTLNITNHSTFRDRSKRQNITNNKSRLFTAVNKL-TGIQTLSSNEKLLLFLITEG 243
Query: 74 QSESTGDLKSLCSQVVSSVLSD 95
+ES +S + +V V D
Sbjct: 242 MTESDLSKRSTATWIVDDVGDD 177
>TC77705 similar to GP|21553857|gb|AAM62950.1 unknown {Arabidopsis
thaliana}, partial (58%)
Length = 1139
Score = 27.7 bits (60), Expect = 4.3
Identities = 32/124 (25%), Positives = 56/124 (44%), Gaps = 4/124 (3%)
Frame = +1
Query: 2 PPQKRKRTKKNLIGKSLTKFTNVSLNITKHLLSNQKLNEKNIVFSPLSLNTVLIMIATGS 61
PPQ +K +++ SL+ + Q++ + V + L++ + T S
Sbjct: 625 PPQVQK-----------VEYSGPSLSYYINEFKTQEITQAPSVSTETELSS---SVPTES 762
Query: 62 EGPTQNQLLSFLQSESTGDLKSLCSQVV--SSVLSDGARAGGPCLS--YVNGVWVEKSLP 117
+ PTQ +L S +QSES K+ S + SV +D + + P S V+ V VE +
Sbjct: 763 DVPTQTELSSSVQSESIAPAKTELSSSIQTESVATDASISVPPESSPAEVSQVQVESQIQ 942
Query: 118 LQPS 121
+ S
Sbjct: 943 TETS 954
>BM815439
Length = 705
Score = 27.7 bits (60), Expect = 4.3
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = -2
Query: 70 LSFLQSESTGDLKSLCSQVVSSVLSDGARAGGPCL 104
L FL+S ++ L+SLCS S S + G CL
Sbjct: 545 LCFLESLTSSSLESLCSTTPSFFSSKSTSSSGVCL 441
>TC82714 similar to PIR|T07142|T07142 beta-glucan-elicitor receptor -
soybean, partial (33%)
Length = 906
Score = 26.9 bits (58), Expect = 7.4
Identities = 14/32 (43%), Positives = 20/32 (61%)
Frame = +1
Query: 184 TSKTKDYDFDLLNGKSVKVPFMTSKNDQFISS 215
T +T DY LLNGK+V + F + QF++S
Sbjct: 397 TRETVDYGLLLLNGKNVGLGFSSYHCFQFLNS 492
>TC78253 weakly similar to GP|22654983|gb|AAM98084.1 AT5g45110/K17O22_11
{Arabidopsis thaliana}, partial (32%)
Length = 1669
Score = 26.9 bits (58), Expect = 7.4
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = +2
Query: 140 VNKAEKKTKGLIQDLLPHGSVNSLTSLIFANALY 173
++K E + K + DLLP+G V LIF + +Y
Sbjct: 1175 LSKNEGRLKYCLSDLLPYGKVGYEAFLIFLSYVY 1276
>TC84816 similar to GP|2313789|gb|AAD07729.1| H. pylori predicted coding
region HP0659 {Helicobacter pylori 26695}, partial (3%)
Length = 807
Score = 26.9 bits (58), Expect = 7.4
Identities = 16/46 (34%), Positives = 23/46 (49%)
Frame = +3
Query: 5 KRKRTKKNLIGKSLTKFTNVSLNITKHLLSNQKLNEKNIVFSPLSL 50
KRK +K NLI S + T + ++ +QKLN+ F SL
Sbjct: 246 KRKNSKNNLIPISSIRATTYLWIVIFKIVCSQKLNKNTHTFRRFSL 383
>TC90831 similar to GP|12831474|gb|AAB05871.2 PAPS-reductase-like protein
{Catharanthus roseus}, partial (14%)
Length = 856
Score = 26.6 bits (57), Expect = 9.6
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = -1
Query: 51 NTVLIMIATGSEGPTQNQLLSFLQ 74
NT L++ S+ P+ NQLLSFL+
Sbjct: 559 NTQLVITLKHSQMPSMNQLLSFLK 488
>BG587125 similar to SP|Q9RQ15|RS16_ 30S ribosomal protein S16. {Bacteroides
thetaiotaomicron}, partial (13%)
Length = 652
Score = 26.6 bits (57), Expect = 9.6
Identities = 31/122 (25%), Positives = 51/122 (41%)
Frame = +2
Query: 19 TKFTNVSLNITKHLLSNQKLNEKNIVFSPLSLNTVLIMIATGSEGPTQNQLLSFLQSEST 78
T F+ S ++L+S + + +++ S +SLN VLI + P + S L S
Sbjct: 113 TFFSTASTLSDRYLISLRHVPNPSLMASAMSLNIVLI---SSLVKPPTVVVTSSLAS--- 274
Query: 79 GDLKSLCSQVVSSVLSDGARAGGPCLSYVNGVWVEKSLPLQPSFKQLMTTDFEATLSAVD 138
+ S + S+ S A AG ++ G+ + TT F A L+ D
Sbjct: 275 ----AAASSLASAAASSLASAGASLRAFFRGLGLSS-----------FTTFFFAGLTTAD 409
Query: 139 FV 140
FV
Sbjct: 410 FV 415
>BE319112 similar to GP|3377842|gb| T27D20.12 gene product {Arabidopsis
thaliana}, partial (76%)
Length = 684
Score = 26.6 bits (57), Expect = 9.6
Identities = 12/32 (37%), Positives = 18/32 (55%)
Frame = +2
Query: 89 VSSVLSDGARAGGPCLSYVNGVWVEKSLPLQP 120
+++VL +G R G VN WV + L +QP
Sbjct: 314 INNVLKEGIRR*GTGFKIVNSNWVRQCL*MQP 409
>TC93048 similar to GP|13516922|dbj|BAB40340. 12-oxophytodienoic acid 10
11-reductase {Pisum sativum}, partial (16%)
Length = 804
Score = 26.6 bits (57), Expect = 9.6
Identities = 15/59 (25%), Positives = 25/59 (41%)
Frame = -1
Query: 87 QVVSSVLSDGARAGGPCLSYVNGVWVEKSLPLQPSFKQLMTTDFEATLSAVDFVNKAEK 145
+V+ S+LS R L VN +W LP+ + FE + + +N A +
Sbjct: 702 EVIKSILSHTTRQHLE*L*VVNQIWTNPKLPMSALAPLMEILSFETRTTKIQNLNMASQ 526
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.317 0.133 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,014,759
Number of Sequences: 36976
Number of extensions: 86123
Number of successful extensions: 510
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 505
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 505
length of query: 249
length of database: 9,014,727
effective HSP length: 94
effective length of query: 155
effective length of database: 5,538,983
effective search space: 858542365
effective search space used: 858542365
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC122166.9