BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122162.5 + phase: 1 /partial
(95 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC84335 weakly similar to GP|21741678|emb|CAD40580. oj000126_13.... 182 2e-47
BE942328 weakly similar to GP|21741678|emb oj000126_13.2 {Oryza ... 118 6e-46
TC89523 weakly similar to GP|17065276|gb|AAL32792.1 Unknown prot... 79 4e-16
TC80442 similar to PIR|F84793|F84793 probable RNA-binding protei... 70 1e-13
BI266384 65 4e-12
AL374421 similar to PIR|S59529|S59 RNA-binding glycine-rich prot... 63 2e-11
AW687209 weakly similar to GP|9757907|dbj| contains similarity t... 62 3e-11
TC80464 weakly similar to GP|13548328|emb|CAC35875. putative pro... 60 1e-10
AL371197 homologue to GP|6273331|gb| glycine-rich RNA binding pr... 59 2e-10
TC76556 similar to PIR|S71779|S71779 glycine-rich RNA-binding pr... 59 2e-10
TC90607 similar to GP|20466724|gb|AAM20679.1 unknown protein {Ar... 59 2e-10
TC79604 similar to GP|18175762|gb|AAL59923.1 unknown protein {Ar... 59 2e-10
CA917955 56 2e-09
TC89001 similar to GP|5726567|gb|AAD48471.1| glycine-rich RNA-bi... 56 3e-09
TC76948 similar to SP|Q08935|ROC1_NICSY 29 kDa ribonucleoprotein... 54 1e-08
TC76789 similar to GP|15294254|gb|AAK95304.1 AT4g24770/F22K18_30... 54 1e-08
TC77756 similar to PIR|T06796|T06796 glycine-rich RNA-binding pr... 54 1e-08
TC76790 similar to PIR|T06817|T06817 RNA-binding protein - garde... 52 4e-08
TC90113 similar to PIR|C86301|C86301 arginine/serine-rich protei... 52 5e-08
TC93504 weakly similar to PIR|S46286|S46286 RNA-binding protein ... 52 5e-08
>TC84335 weakly similar to GP|21741678|emb|CAD40580. oj000126_13.2 {Oryza
sativa}, partial (39%)
Length = 678
Score = 182 bits (461), Expect = 2e-47
Identities = 90/90 (100%), Positives = 90/90 (100%)
Frame = +2
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL
Sbjct: 221 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 400
Query: 61 HGRVLYVDMDPPNEQKNKIKQATKNTEVDN 90
HGRVLYVDMDPPNEQKNKIKQATKNTEVDN
Sbjct: 401 HGRVLYVDMDPPNEQKNKIKQATKNTEVDN 490
>BE942328 weakly similar to GP|21741678|emb oj000126_13.2 {Oryza sativa},
partial (34%)
Length = 548
Score = 118 bits (295), Expect(2) = 6e-46
Identities = 58/59 (98%), Positives = 59/59 (99%)
Frame = +3
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKI 59
LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGK+
Sbjct: 135 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKV 311
Score = 80.9 bits (198), Expect(2) = 6e-46
Identities = 37/38 (97%), Positives = 38/38 (99%)
Frame = +1
Query: 58 KILHGRVLYVDMDPPNEQKNKIKQATKNTEVDNEDVHM 95
+ILHGRVLYVDMDPPNEQKNKIKQATKNTEVDNEDVHM
Sbjct: 388 QILHGRVLYVDMDPPNEQKNKIKQATKNTEVDNEDVHM 501
>TC89523 weakly similar to GP|17065276|gb|AAL32792.1 Unknown protein
{Arabidopsis thaliana}, partial (64%)
Length = 729
Score = 78.6 bits (192), Expect = 4e-16
Identities = 35/77 (45%), Positives = 57/77 (73%)
Frame = +3
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
L+F TTE+ L++AFS YG VV+A ++ ++ ++ KG+G+VTFA ++EA KA MN K L
Sbjct: 147 LSFHTTEKTLSDAFSNYGQVVEAKVITDRISEKSKGYGFVTFASQDEAEKAITEMNEKAL 326
Query: 61 HGRVLYVDMDPPNEQKN 77
+GRV++VD P+ +++
Sbjct: 327 NGRVVFVDYAKPDTKRS 377
>TC80442 similar to PIR|F84793|F84793 probable RNA-binding protein
[imported] - Arabidopsis thaliana, partial (64%)
Length = 894
Score = 70.1 bits (170), Expect = 1e-13
Identities = 32/72 (44%), Positives = 49/72 (67%)
Frame = +1
Query: 5 TTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKILHGRV 64
TT+EKL EAFS +G +++A ++ ++ R KGF +V+++ EEA KA+ GMN K L G V
Sbjct: 322 TTDEKLTEAFSPFGQLLEAKVITDRGSGRSKGFAFVSYSTIEEAEKAREGMNAKFLDGWV 501
Query: 65 LYVDMDPPNEQK 76
++VD P E +
Sbjct: 502 IFVDPAKPREPR 537
>BI266384
Length = 626
Score = 65.1 bits (157), Expect = 4e-12
Identities = 33/95 (34%), Positives = 53/95 (55%)
Frame = +2
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
L STTE L + FS +G V + ++++K + GF Y+ F EE+ A+ A MNGK+
Sbjct: 230 LPLSTTELHLTKVFSMFGEVTQVKLLIDKKTGQSLGFAYIWFVEEDSAQSAAKEMNGKLF 409
Query: 61 HGRVLYVDMDPPNEQKNKIKQATKNTEVDNEDVHM 95
+GR +YV + P K K ++ + +D + V M
Sbjct: 410 YGRFIYVTIAKPGSSK-KFEEKSLQILIDMDSVSM 511
>AL374421 similar to PIR|S59529|S59 RNA-binding glycine-rich protein-1
(RGP-1c) - wood tobacco, partial (62%)
Length = 310
Score = 63.2 bits (152), Expect = 2e-11
Identities = 30/68 (44%), Positives = 47/68 (69%)
Frame = +2
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
LA++T E L +AFSQYG ++ + I+ ++ R +GFG+VTFA+E+ R A GMNG+ +
Sbjct: 35 LAWATDSEALEKAFSQYGEIIDSKIINDRETGRSRGFGFVTFADEKSMRDAIEGMNGQDM 214
Query: 61 HGRVLYVD 68
GR + V+
Sbjct: 215 DGRNITVN 238
>AW687209 weakly similar to GP|9757907|dbj| contains similarity to RNA
binding protein~gene_id:MMN10.9 {Arabidopsis thaliana},
partial (64%)
Length = 531
Score = 62.4 bits (150), Expect = 3e-11
Identities = 27/74 (36%), Positives = 51/74 (68%)
Frame = +2
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
L+F TT+++L FS +G + +A ++ ++ +R KGFG+V++ E EA KA+ +NG+I+
Sbjct: 209 LSFYTTQQQLESLFSPFGVLTEATLITDQNTQRPKGFGFVSYKSEIEAEKARKALNGRIV 388
Query: 61 HGRVLYVDMDPPNE 74
GR+++V+ P +
Sbjct: 389 DGRLIFVEHAKPKD 430
>TC80464 weakly similar to GP|13548328|emb|CAC35875. putative protein
{Arabidopsis thaliana}, partial (19%)
Length = 1437
Score = 60.5 bits (145), Expect = 1e-10
Identities = 32/78 (41%), Positives = 47/78 (60%)
Frame = +3
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
L +STTEE+L E FSQ+G+V +A +V++K KR KG Y+ F+ E A +A + I
Sbjct: 1020 LPYSTTEEELEEHFSQFGSVSQAHLVVDKDTKRSKGIAYIHFSAPEFAARALEESDNSIF 1199
Query: 61 HGRVLYVDMDPPNEQKNK 78
GR+L+V P N+
Sbjct: 1200 QGRLLHVMPAIPRHSNNE 1253
>AL371197 homologue to GP|6273331|gb| glycine-rich RNA binding protein
{Medicago sativa}, complete
Length = 437
Score = 59.3 bits (142), Expect = 2e-10
Identities = 30/68 (44%), Positives = 44/68 (64%)
Frame = +1
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
LA++T E L +AFSQYG +V + I+ ++ R +GFG+VTFA E+ A MNG+ L
Sbjct: 79 LAWATDNEALEKAFSQYGEIVDSKIINDRETGRSRGFGFVTFANEKSMNDAIEAMNGQDL 258
Query: 61 HGRVLYVD 68
GR + V+
Sbjct: 259 DGRNITVN 282
>TC76556 similar to PIR|S71779|S71779 glycine-rich RNA-binding protein GRP1
- wheat, partial (95%)
Length = 754
Score = 59.3 bits (142), Expect = 2e-10
Identities = 30/68 (44%), Positives = 44/68 (64%)
Frame = +2
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
LA++T E L +AFSQYG +V + I+ ++ R +GFG+VTFA E+ A MNG+ L
Sbjct: 122 LAWATDNEALEKAFSQYGEIVDSKIINDRETGRSRGFGFVTFANEKSMNDAIEAMNGQDL 301
Query: 61 HGRVLYVD 68
GR + V+
Sbjct: 302 DGRNITVN 325
>TC90607 similar to GP|20466724|gb|AAM20679.1 unknown protein {Arabidopsis
thaliana}, partial (49%)
Length = 1143
Score = 59.3 bits (142), Expect = 2e-10
Identities = 29/79 (36%), Positives = 48/79 (60%), Gaps = 3/79 (3%)
Frame = +1
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
L+F T+E+ L AF +G +V+ ++++K KR KG+ ++ + EE A A MNGKI+
Sbjct: 598 LSFYTSEKTLRAAFEGFGELVEVKVIIDKISKRSKGYAFIEYTTEEAASAALKEMNGKII 777
Query: 61 HGRVLYVDM---DPPNEQK 76
+G ++ VD+ PP K
Sbjct: 778 NGWMIVVDVAKTTPPRYNK 834
>TC79604 similar to GP|18175762|gb|AAL59923.1 unknown protein {Arabidopsis
thaliana}, partial (56%)
Length = 727
Score = 59.3 bits (142), Expect = 2e-10
Identities = 33/81 (40%), Positives = 46/81 (56%), Gaps = 4/81 (4%)
Frame = +2
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
L+ TT E L EAF ++G VV A +V ++ KGFG+V +A E+A K GM+GK L
Sbjct: 260 LSKRTTTETLREAFQKFGEVVHARVVTDRVSGYSKGFGFVKYATLEDAAKGIEGMDGKFL 439
Query: 61 HGRVLYVD----MDPPNEQKN 77
G V++ + PP E N
Sbjct: 440 EGWVIFAEYARPRPPPGEAVN 502
>CA917955
Length = 870
Score = 56.2 bits (134), Expect = 2e-09
Identities = 31/97 (31%), Positives = 50/97 (50%), Gaps = 13/97 (13%)
Frame = +2
Query: 1 LAFSTTEEKLAEAFSQYGNVVKAD-------------IVLNKAKKRCKGFGYVTFAEEEE 47
L STTE L + FS +G V + + ++++K + GF Y+ F EE+
Sbjct: 173 LPLSTTELHLTKVFSMFGEVTQGN*L*IYFGCFQRVKLLIDKKTGQSLGFAYIWFVEEDS 352
Query: 48 ARKAQIGMNGKILHGRVLYVDMDPPNEQKNKIKQATK 84
A+ A MNGK+ +GR +YV + P K+ ++A K
Sbjct: 353 AQSAAKEMNGKLFYGRFIYVTIAKPGSSKSLKRKAYK 463
>TC89001 similar to GP|5726567|gb|AAD48471.1| glycine-rich RNA-binding
protein {Glycine max}, partial (90%)
Length = 488
Score = 55.8 bits (133), Expect = 3e-09
Identities = 26/68 (38%), Positives = 45/68 (65%)
Frame = +3
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
LA++T + L +AFS++G + + ++ ++ R +GFG+VTFAEE+ R A MNG+ +
Sbjct: 72 LAWATDSQALEQAFSKFGEITDSKVINDRETGRSRGFGFVTFAEEKSMRDAIEEMNGQDI 251
Query: 61 HGRVLYVD 68
GR + V+
Sbjct: 252 DGRNITVN 275
>TC76948 similar to SP|Q08935|ROC1_NICSY 29 kDa ribonucleoprotein A
chloroplast precursor (CP29A). [Wood tobacco] {Nicotiana
sylvestris}, partial (61%)
Length = 1374
Score = 53.9 bits (128), Expect = 1e-08
Identities = 27/85 (31%), Positives = 46/85 (53%)
Frame = +1
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
L FS +LAE F G+V +++ +K+ R +GFG+VT + E A +NG ++
Sbjct: 361 LPFSVDSAQLAEIFENAGDVEMVEVIYDKSTGRSRGFGFVTMSSAAEVEAAAQQLNGYVV 540
Query: 61 HGRVLYVDMDPPNEQKNKIKQATKN 85
GR L V+ PP +++ + +N
Sbjct: 541 DGRELRVNAGPPPPPRSENSRFGEN 615
Score = 43.5 bits (101), Expect = 1e-05
Identities = 21/67 (31%), Positives = 37/67 (54%)
Frame = +1
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
LA+ L F + G V++A ++ ++ R +GFG+VTF+ +E A ++G L
Sbjct: 697 LAWGVDNLALESLFGEQGQVLEAKVIYDRESGRSRGFGFVTFSSADEVDSAIRTLDGADL 876
Query: 61 HGRVLYV 67
+GR + V
Sbjct: 877 NGRAIRV 897
>TC76789 similar to GP|15294254|gb|AAK95304.1 AT4g24770/F22K18_30
{Arabidopsis thaliana}, partial (62%)
Length = 1294
Score = 53.5 bits (127), Expect = 1e-08
Identities = 28/72 (38%), Positives = 40/72 (54%)
Frame = +3
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
L F EKLA+ F Q G V A+++ N+ R +GFG+VT + EE +A +G L
Sbjct: 474 LPFDVDSEKLAQLFEQSGTVEIAEVIYNRDTDRSRGFGFVTMSTSEEVERAVNKFSGFEL 653
Query: 61 HGRVLYVDMDPP 72
GR+L V+ P
Sbjct: 654 DGRLLTVNKAAP 689
Score = 46.2 bits (108), Expect = 2e-06
Identities = 21/69 (30%), Positives = 38/69 (54%)
Frame = +3
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
L + L + FS++G V A +V ++ R +GFG+VT + E E A ++G+
Sbjct: 756 LPWDVDNSSLEQLFSEHGKVESAQVVYDRETGRSRGFGFVTMSNEAEMNDAIAALDGQSF 935
Query: 61 HGRVLYVDM 69
+GR + V++
Sbjct: 936 NGRAIRVNV 962
>TC77756 similar to PIR|T06796|T06796 glycine-rich RNA-binding protein -
garden pea, partial (89%)
Length = 813
Score = 53.5 bits (127), Expect = 1e-08
Identities = 25/67 (37%), Positives = 45/67 (66%)
Frame = +3
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
L+++ ++ L +AF+ YG+VV+A ++ ++ R +GFG+V F EE A A + M+G+ L
Sbjct: 207 LSYNVDDQSLRDAFTTYGDVVEARVITDRETGRSRGFGFVNFTSEESATSA-LSMDGQDL 383
Query: 61 HGRVLYV 67
+GR + V
Sbjct: 384 NGRNIRV 404
>TC76790 similar to PIR|T06817|T06817 RNA-binding protein - garden pea,
partial (79%)
Length = 1141
Score = 52.0 bits (123), Expect = 4e-08
Identities = 23/69 (33%), Positives = 41/69 (59%)
Frame = +1
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
LA+ +L + FS++G +V A +V ++ R +GFG+VT ++E E A ++G+ L
Sbjct: 673 LAWEVDNSRLEQVFSEHGKIVSARVVYDRETGRSRGFGFVTMSDETEMNDAIAALDGQSL 852
Query: 61 HGRVLYVDM 69
GR + V +
Sbjct: 853 EGRTIRVSV 879
Score = 49.3 bits (116), Expect = 2e-07
Identities = 29/86 (33%), Positives = 43/86 (49%), Gaps = 1/86 (1%)
Frame = +1
Query: 3 FSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKILHG 62
F EKLA F Q G V A+++ N+ +GFG+VT + EEA A NG +G
Sbjct: 388 FDVDSEKLAMLFGQAGTVEIAEVIYNRQTDLSRGFGFVTMSTVEEAESAVEKFNGYDYNG 567
Query: 63 RVLYVD-MDPPNEQKNKIKQATKNTE 87
R L V+ P + + ++A + E
Sbjct: 568 RSLVVNKASPKGSRPERTERAPRTFE 645
>TC90113 similar to PIR|C86301|C86301 arginine/serine-rich protein
[imported] - Arabidopsis thaliana, partial (70%)
Length = 1259
Score = 51.6 bits (122), Expect = 5e-08
Identities = 28/76 (36%), Positives = 41/76 (53%)
Frame = +1
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
L+ + E L E FS +G VV ++V+++A KG+GYV F EA KA + M+G +
Sbjct: 283 LSRNVNEGHLKEIFSNFGEVVSVELVMDRAVNLPKGYGYVHFKTRGEAEKALLYMDGAQI 462
Query: 61 HGRVLYVDMDPPNEQK 76
G V+ P QK
Sbjct: 463 DGNVVKARFTLPPRQK 510
>TC93504 weakly similar to PIR|S46286|S46286 RNA-binding protein - wood
tobacco, partial (55%)
Length = 576
Score = 51.6 bits (122), Expect = 5e-08
Identities = 24/57 (42%), Positives = 38/57 (66%)
Frame = +3
Query: 12 EAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKILHGRVLYVD 68
+ FS+YG+V+ A I++++ R +GFG++TFA EEA A M+ K L GR + V+
Sbjct: 195 KXFSRYGDVLDAKIIMDRDTGRSRGFGFITFATSEEASSALQAMDNKELDGRTVRVN 365
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.312 0.130 0.356
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,996,568
Number of Sequences: 36976
Number of extensions: 17669
Number of successful extensions: 216
Number of sequences better than 10.0: 131
Number of HSP's better than 10.0 without gapping: 186
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 214
length of query: 95
length of database: 9,014,727
effective HSP length: 71
effective length of query: 24
effective length of database: 6,389,431
effective search space: 153346344
effective search space used: 153346344
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 51 (24.3 bits)
Medicago: description of AC122162.5