BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122162.11 - phase: 0
(208 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BM815798 homologue to GP|7292702|gb|A CG15741 gene product {Dros... 211 1e-55
TC89339 similar to GP|17380986|gb|AAL36305.1 unknown protein {Ar... 156 6e-39
TC81938 similar to PIR|C84547|C84547 hypothetical protein At2g17... 111 2e-25
CB893128 weakly similar to PIR|F96671|F966 hypothetical protein ... 80 4e-16
TC82598 weakly similar to PIR|C84547|C84547 hypothetical protein... 64 5e-15
TC89716 69 2e-12
TC84584 similar to PIR|C84547|C84547 hypothetical protein At2g17... 64 3e-11
BE999602 weakly similar to GP|6686405|gb|A F1E22.11 {Arabidopsis... 50 5e-07
AL381637 weakly similar to PIR|C96738|C967 unknown protein F3I17... 46 1e-05
BE319225 45 2e-05
TC89529 homologue to PIR|H86400|H86400 protein T17H3.7 [imported... 36 0.012
TC86447 weakly similar to GP|19911585|dbj|BAB86896. syringolide-... 30 0.86
TC88273 similar to GP|15293103|gb|AAK93662.1 unknown protein {Ar... 28 1.9
TC77013 similar to GP|7025485|gb|AAF35897.1| pectin methylestera... 27 5.6
TC86861 similar to GP|21741562|emb|CAD39524. OSJNBa0027O01.12 {O... 27 5.6
TC77012 homologue to SP|P54776|PRSA_LYCES 26S protease regulator... 27 5.6
BG585459 similar to GP|1708781|emb LMP7-like protein {Botryllus ... 26 9.5
>BM815798 homologue to GP|7292702|gb|A CG15741 gene product {Drosophila
melanogaster}, partial (14%)
Length = 577
Score = 211 bits (538), Expect = 1e-55
Identities = 106/139 (76%), Positives = 117/139 (83%), Gaps = 4/139 (2%)
Frame = +3
Query: 74 PLDPGIPGLNKMLVESEGRLLLLAINEMFYSFSIDFFKLDEKEKKWVRLMDFDEKEKKWV 133
PL PG NKMLVESEGRLLLL I E FSIDFF+LDEK+KKWVRLMDFDEKEKKWV
Sbjct: 6 PLGLDSPGWNKMLVESEGRLLLLGIEESSNYFSIDFFELDEKKKKWVRLMDFDEKEKKWV 185
Query: 134 KLRHFGDRIFFIGRGCSFSASASDLCIPKGNCVVFIDETVLWIDN----KRVFHLDQNQL 189
KLR+FGDR+FFIGRGCSFSASASDLCI KGNC +FIDE+VL +N KRVFHLDQ++L
Sbjct: 186 KLRNFGDRVFFIGRGCSFSASASDLCIQKGNCAIFIDESVLHNNNMVRGKRVFHLDQDRL 365
Query: 190 SCGAKYLNLFLPPKWIHNI 208
S G+KYLNLFLPP+WI I
Sbjct: 366 SRGSKYLNLFLPPEWILKI 422
>TC89339 similar to GP|17380986|gb|AAL36305.1 unknown protein {Arabidopsis
thaliana}, partial (3%)
Length = 1290
Score = 156 bits (394), Expect = 6e-39
Identities = 96/213 (45%), Positives = 131/213 (61%), Gaps = 9/213 (4%)
Frame = +3
Query: 2 CHGKNPLLLGRLSHRFNRRIIFRGLDEQWKLISKRSYEYGDICLFKGRIYAVNQSGETVT 61
CHGKNPL++ +S ++ + +E+WK+I S E+GDICLFKGR YAV++ G+T+
Sbjct: 441 CHGKNPLIVATISSH-PEPLLSKCGNEKWKVIPDMSMEFGDICLFKGRAYAVDKIGKTIM 617
Query: 62 VGPDSSVELAAQPLDPGIPGLNKMLVESEGRLLLLAINEMFYSFSIDFFKLDEKEKKWVR 121
VG DS+V L A+PLD G K+LVES+G LLL ++ F S+D FKL+EK
Sbjct: 618 VGSDSNVHLVAEPLDGG--ENKKLLVESDGDLLLACVH-AFPVISVDLFKLNEK------ 770
Query: 122 LMDFDEKEKKWVKLRHFGDRIFFIGRGCSFSASASDLCIPKGNCVVF---IDETVLWIDN 178
EKKWVKL GD++ F+G CSFSAS SDL + KGNCV+ I + + +
Sbjct: 771 -------EKKWVKLTSLGDKVLFLGIKCSFSASFSDLYVAKGNCVIISADIFRRLSRLGS 929
Query: 179 KRVFHLDQNQ----LSCGA-KYLNLFL-PPKWI 205
+ + LD +Q L C + +Y NLF PPKWI
Sbjct: 930 QASYVLDLDQGWLPLLCDSPEYSNLFWPPPKWI 1028
>TC81938 similar to PIR|C84547|C84547 hypothetical protein At2g17030
[imported] - Arabidopsis thaliana, partial (1%)
Length = 916
Score = 111 bits (278), Expect = 2e-25
Identities = 70/174 (40%), Positives = 95/174 (54%), Gaps = 9/174 (5%)
Frame = +1
Query: 41 GDICLFKGRIYAVNQSGETVTVGPDSSVELAAQPLDPGIPGLNKMLVESEGRLLLLAINE 100
G++C+FKGR V+++G T +GPD + L A+P+ G +K LVES LLL
Sbjct: 28 GNVCVFKGRPCVVDKTGRTFMIGPDLTTHLIAEPV---FGGKSKFLVESSEFELLLVDRY 198
Query: 101 MFYSFSIDFFKLDEKEKKWVRLMDFDEKEKKWVKLRHFGDRIFFIGRGCSFSASASDLCI 160
Y + W+ ++ DEKEK+WVKL + GD++ F+G G SFSASAS+L
Sbjct: 199 ENYRVPV-----------WIDVLRLDEKEKRWVKLANLGDKVLFLGNGYSFSASASELGF 345
Query: 161 PKGNCVVFIDETVLWIDNKR-----VFHLDQNQ---LSCGAKYLNLFL-PPKWI 205
GNCV++ N R VFHLDQ Q LS +Y LF PP+WI
Sbjct: 346 ANGNCVIYSKSYGFHDLNIRKCKMSVFHLDQGQASPLSDYPEYFKLFWPPPEWI 507
>CB893128 weakly similar to PIR|F96671|F966 hypothetical protein F13O11.14
[imported] - Arabidopsis thaliana, partial (8%)
Length = 768
Score = 80.5 bits (197), Expect = 4e-16
Identities = 38/58 (65%), Positives = 41/58 (70%)
Frame = +3
Query: 2 CHGKNPLLLGRLSHRFNRRIIFRGLDEQWKLISKRSYEYGDICLFKGRIYAVNQSGET 59
CHGKNPLLLG LSH + +IFRG +EQWKL S S GDICLFKGR Y V SG T
Sbjct: 594 CHGKNPLLLGTLSHFSHLPVIFRGCNEQWKLFSNLSTVRGDICLFKGRFYVVEMSGRT 767
>TC82598 weakly similar to PIR|C84547|C84547 hypothetical protein At2g17030
[imported] - Arabidopsis thaliana, partial (2%)
Length = 1050
Score = 64.3 bits (155), Expect(2) = 5e-15
Identities = 40/100 (40%), Positives = 56/100 (56%), Gaps = 7/100 (7%)
Frame = +2
Query: 27 DEQWKLISKRSYEYGDICLFKGRIYAVNQSGETVTVGPDSSVELAAQPLDPGIPGLNKML 86
++ W LI S + DIC+FK R YAVN+ G TV G D SVEL A+ ++ G K L
Sbjct: 677 EKHWTLIPCMSTYFLDICVFKERFYAVNKIGRTVAFGLDYSVELVAEYVNGGD---MKFL 847
Query: 87 VESEGRLLLLAINEMF-------YSFSIDFFKLDEKEKKW 119
VE EG LLL+ I + + F+L+E++K+W
Sbjct: 848 VECEGELLLVDICDSHCFGFPGEKGLKLKVFRLNERKKEW 967
Score = 32.7 bits (73), Expect(2) = 5e-15
Identities = 14/20 (70%), Positives = 16/20 (80%)
Frame = +3
Query: 139 GDRIFFIGRGCSFSASASDL 158
G + F+G GCSFSASASDL
Sbjct: 984 GIGVLFLGNGCSFSASASDL 1043
>TC89716
Length = 735
Score = 68.6 bits (166), Expect = 2e-12
Identities = 46/92 (50%), Positives = 56/92 (60%), Gaps = 12/92 (13%)
Frame = +2
Query: 126 DEKEKKWVKLRHFGDRIFFIGRG--CSFSASASDLCIPKGNCVVF---IDETVL---WID 177
+EKEKKWVKL GDRI F+G G CSFSA ASDLC+ KGN V+F I E+ +
Sbjct: 8 NEKEKKWVKLTSLGDRILFLGLGLVCSFSACASDLCVAKGNSVIFNNYIFESYRPFGFQC 187
Query: 178 NKRVFHLDQNQ---LSCGAKYLNLFLPP-KWI 205
+ V LDQ + LS +Y NL PP +WI
Sbjct: 188 KQYVLDLDQGRHSLLSDCPEYSNLLWPPAEWI 283
>TC84584 similar to PIR|C84547|C84547 hypothetical protein At2g17030
[imported] - Arabidopsis thaliana, partial (4%)
Length = 812
Score = 64.3 bits (155), Expect = 3e-11
Identities = 33/82 (40%), Positives = 51/82 (61%)
Frame = +3
Query: 15 HRFNRRIIFRGLDEQWKLISKRSYEYGDICLFKGRIYAVNQSGETVTVGPDSSVELAAQP 74
H + +FR DE+W ++ + + D+C+FKGR AV+ +G TV + PD S++L A+P
Sbjct: 573 HISGKIALFRCGDERWTIVPEMPSPFDDVCVFKGRPVAVDGTGRTVALRPDLSLDLVAEP 752
Query: 75 LDPGIPGLNKMLVESEGRLLLL 96
+ G K LVES+G LLL+
Sbjct: 753 V---FGGDKKFLVESDGELLLV 809
>BE999602 weakly similar to GP|6686405|gb|A F1E22.11 {Arabidopsis thaliana},
partial (4%)
Length = 579
Score = 50.4 bits (119), Expect = 5e-07
Identities = 40/162 (24%), Positives = 75/162 (45%), Gaps = 6/162 (3%)
Frame = +1
Query: 35 KRSYEYGDICLFKGRIYAVNQSGETVTVGPDSSVELAAQPLDPGIPGLNKMLVESEGRLL 94
+ ++ Y D+ +FKG+ Y ++ G + S + P G G K LVES G L
Sbjct: 1 ENNFYYDDLIVFKGQFYVTDKWGTISWIDISSLKLIQFSPPLCGF-GNKKHLVESCGNLY 177
Query: 95 LL------AINEMFYSFSIDFFKLDEKEKKWVRLMDFDEKEKKWVKLRHFGDRIFFIGRG 148
++ M ++ + + + ++ DE+ KWV ++ +R F +
Sbjct: 178 VVDRYSYYEDENMGGNYDGRRHQARYEVVECFKVYKLDEEWGKWVDVKDLKNRAFILSPS 357
Query: 149 CSFSASASDLCIPKGNCVVFIDETVLWIDNKRVFHLDQNQLS 190
C+FS SA +L +GNC+ F D + R+++LD +++
Sbjct: 358 CNFSVSAKELIGYQGNCIYFKDSF-----SVRMYNLDDCRIT 468
>AL381637 weakly similar to PIR|C96738|C967 unknown protein F3I17.1
[imported] - Arabidopsis thaliana, partial (9%)
Length = 508
Score = 45.8 bits (107), Expect = 1e-05
Identities = 32/69 (46%), Positives = 37/69 (53%), Gaps = 9/69 (13%)
Frame = +3
Query: 146 GRGCSFSASASDLCIPKGNCVVFIDETVLWIDNKR-----VFHLDQNQ---LSCGAKYLN 197
G G SFSASAS+L GNCV++ N R VFHLDQ Q LS +Y
Sbjct: 3 GNGYSFSASASELGFANGNCVIYSKSYGFHDLNIRKCKMSVFHLDQGQASPLSDYPEYFK 182
Query: 198 LFL-PPKWI 205
LF PP+WI
Sbjct: 183 LFWPPPEWI 209
>BE319225
Length = 242
Score = 45.4 bits (106), Expect = 2e-05
Identities = 27/55 (49%), Positives = 36/55 (65%), Gaps = 1/55 (1%)
Frame = +2
Query: 40 YGDICLFKGRIYAVNQSGETVTVG-PDSSVELAAQPLDPGIPGLNKMLVESEGRL 93
+ DIC+FKGR Y V+++G T T+G D + EL A+ L G G K LVES+G L
Sbjct: 80 FEDICIFKGRPYVVHKTGRTATLGLDDFTFELVAESLVSG-GGDIKFLVESDGDL 241
>TC89529 homologue to PIR|H86400|H86400 protein T17H3.7 [imported] -
Arabidopsis thaliana, partial (1%)
Length = 1745
Score = 35.8 bits (81), Expect = 0.012
Identities = 20/57 (35%), Positives = 32/57 (56%), Gaps = 2/57 (3%)
Frame = +1
Query: 117 KKWVRLMDFDEKEKKWVKLRHFGDRIFFIGRGCSFSASASDL--CIPKGNCVVFIDE 171
K W+ M+++ KW +++ G+ FIG+ S S SA+D C P NC+ F D+
Sbjct: 1228 KFWIYKMNWNML--KWEEIQTLGEHSLFIGKNYSLSFSAADFAGCCP--NCIYFTDD 1386
>TC86447 weakly similar to GP|19911585|dbj|BAB86896. syringolide-induced
protein 13-1-1 {Glycine max}, partial (36%)
Length = 1223
Score = 29.6 bits (65), Expect = 0.86
Identities = 18/55 (32%), Positives = 29/55 (52%)
Frame = +2
Query: 78 GIPGLNKMLVESEGRLLLLAINEMFYSFSIDFFKLDEKEKKWVRLMDFDEKEKKW 132
G+ ++KM++ L + MF SF FFKL ++K W+ ++ D KKW
Sbjct: 551 GVLKIDKMVLRGFQLLEYQLVRSMFPSF---FFKLKNQQKVWINMVVLD-WFKKW 703
>TC88273 similar to GP|15293103|gb|AAK93662.1 unknown protein {Arabidopsis
thaliana}, partial (30%)
Length = 1340
Score = 28.5 bits (62), Expect = 1.9
Identities = 19/57 (33%), Positives = 27/57 (47%), Gaps = 11/57 (19%)
Frame = -1
Query: 139 GDRIFFIGRGCSFSASASDLC----------IPKGNCVVF-IDETVLWIDNKRVFHL 184
G +FF G G AS+ LC + +G+ V F +D V WI + VFH+
Sbjct: 1142 GSVLFFRGMGLQVKASSM*LCTKRVRKSIMLLKRGDKVEFSVDGEVSWIFREMVFHI 972
>TC77013 similar to GP|7025485|gb|AAF35897.1| pectin methylesterase isoform
alpha {Vigna radiata}, partial (94%)
Length = 1655
Score = 26.9 bits (58), Expect = 5.6
Identities = 12/28 (42%), Positives = 14/28 (49%)
Frame = +1
Query: 144 FIGRGCSFSASASDLCIPKGNCVVFIDE 171
FIG G A L K C++FIDE
Sbjct: 1390 FIGDGAKLVRDAFQLAKEKSPCIIFIDE 1473
>TC86861 similar to GP|21741562|emb|CAD39524. OSJNBa0027O01.12 {Oryza
sativa}, partial (82%)
Length = 893
Score = 26.9 bits (58), Expect = 5.6
Identities = 20/69 (28%), Positives = 32/69 (45%)
Frame = +3
Query: 78 GIPGLNKMLVESEGRLLLLAINEMFYSFSIDFFKLDEKEKKWVRLMDFDEKEKKWVKLRH 137
G+ G+ + S G LLLL + DF+ D ++K++ +L K +
Sbjct: 309 GLGGVLFIFGSSFGALLLLLHQLIATPIRYDFYNYDNEDKEFTQLF-----IKFTQNMAL 473
Query: 138 FGDRIFFIG 146
FG +FFIG
Sbjct: 474 FGALLFFIG 500
>TC77012 homologue to SP|P54776|PRSA_LYCES 26S protease regulatory subunit
6A homolog (TAT-binding protein homolog 1) (TBP-1)
(Mg(2+), complete
Length = 1793
Score = 26.9 bits (58), Expect = 5.6
Identities = 12/28 (42%), Positives = 14/28 (49%)
Frame = +1
Query: 144 FIGRGCSFSASASDLCIPKGNCVVFIDE 171
FIG G A L K C++FIDE
Sbjct: 862 FIGDGAKLVRDAFQLAKEKSPCIIFIDE 945
>BG585459 similar to GP|1708781|emb LMP7-like protein {Botryllus schlosseri},
partial (74%)
Length = 643
Score = 26.2 bits (56), Expect = 9.5
Identities = 11/29 (37%), Positives = 15/29 (50%)
Frame = +1
Query: 132 WVKLRHFGDRIFFIGRGCSFSASASDLCI 160
W++LRH R F IG+ S + CI
Sbjct: 460 WIRLRHVSSRCFRIGQTFGLSCCS*RCCI 546
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.325 0.144 0.457
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,134,781
Number of Sequences: 36976
Number of extensions: 124677
Number of successful extensions: 647
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 630
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 631
length of query: 208
length of database: 9,014,727
effective HSP length: 92
effective length of query: 116
effective length of database: 5,612,935
effective search space: 651100460
effective search space used: 651100460
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 56 (26.2 bits)
Medicago: description of AC122162.11