BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121242.7 + phase: 0
(435 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC88726 similar to GP|11994239|dbj|BAB01361. gb|AAD27719.1~gene_... 426 0.0
BQ139044 similar to GP|9857294|dbj CpABA1 {Vigna unguiculata}, p... 48 8e-06
BI271514 similar to GP|9857294|dbj CpABA1 {Vigna unguiculata}, p... 47 1e-05
TC91982 weakly similar to GP|170525|gb|AAA34202.1|| phenol hydro... 39 0.003
TC80583 squalene monooxygenase 1 [Medicago truncatula] 37 0.019
TC79960 similar to PIR|T52419|T52419 CTF2A protein [imported] - ... 35 0.074
TC76999 homologue to GP|19749359|gb|AAD28640.2 geranylgeranyl hy... 35 0.074
TC88941 similar to PIR|T52419|T52419 CTF2A protein [imported] - ... 34 0.097
TC77092 squalene monooxygenase 2 [Medicago truncatula] 33 0.22
TC82836 similar to PIR|T05682|T05682 monooxygenase (EC 1.-.-.-) ... 33 0.22
TC86756 weakly similar to GP|18253083|dbj|BAB83948. CIG1 {Nicoti... 31 0.82
TC88192 weakly similar to PIR|T05682|T05682 monooxygenase (EC 1.... 31 1.1
BM813592 similar to GP|20161555|db putative homeodomain-leucine ... 30 2.4
BF004918 similar to GP|21553624|gb unknown {Arabidopsis thaliana... 28 5.3
TC86353 similar to GP|15450733|gb|AAK96638.1 At2g36220/F2H17.17 ... 28 5.3
TC76738 28 9.0
>TC88726 similar to GP|11994239|dbj|BAB01361.
gb|AAD27719.1~gene_id:MUJ8.10~similar to unknown protein
{Arabidopsis thaliana}, partial (58%)
Length = 2001
Score = 426 bits (1094), Expect(3) = 0.0
Identities = 207/207 (100%), Positives = 207/207 (100%)
Frame = +3
Query: 1 MPMTKQLSVAIIDSNPALSSGLCIKKEDPPDPRVSTVTPASISFLQEAGAWKYVEQNRHA 60
MPMTKQLSVAIIDSNPALSSGLCIKKEDPPDPRVSTVTPASISFLQEAGAWKYVEQNRHA
Sbjct: 456 MPMTKQLSVAIIDSNPALSSGLCIKKEDPPDPRVSTVTPASISFLQEAGAWKYVEQNRHA 635
Query: 61 YFKKMQVWDYTGFGYARYDAGDVNKDFLGCVAENKVLHSSLLSCIKDSDFKTTFYPSRLT 120
YFKKMQVWDYTGFGYARYDAGDVNKDFLGCVAENKVLHSSLLSCIKDSDFKTTFYPSRLT
Sbjct: 636 YFKKMQVWDYTGFGYARYDAGDVNKDFLGCVAENKVLHSSLLSCIKDSDFKTTFYPSRLT 815
Query: 121 SMTLNTNSMSVVEENTPSKEPSPAQGHASKLELSDGSSIYAKLVVGADGGKSRVRELAGF 180
SMTLNTNSMSVVEENTPSKEPSPAQGHASKLELSDGSSIYAKLVVGADGGKSRVRELAGF
Sbjct: 816 SMTLNTNSMSVVEENTPSKEPSPAQGHASKLELSDGSSIYAKLVVGADGGKSRVRELAGF 995
Query: 181 KTTGWNYSQNAIICTVEHTSANHCAWQ 207
KTTGWNYSQNAIICTVEHTSANHCAWQ
Sbjct: 996 KTTGWNYSQNAIICTVEHTSANHCAWQ 1076
Score = 399 bits (1026), Expect(3) = 0.0
Identities = 201/202 (99%), Positives = 201/202 (99%)
Frame = +2
Query: 234 TESNDRKSITEEMFLKDVNSALDYGYGPHPTSSSLGARDIFSWLKMDGTLSANEPFEIPP 293
TESNDRKSITEEMFLKDVNSALDYGYGPHPTSSSLGARDIFSWLKMDGTLSANEPFEIPP
Sbjct: 1157 TESNDRKSITEEMFLKDVNSALDYGYGPHPTSSSLGARDIFSWLKMDGTLSANEPFEIPP 1336
Query: 294 KAIRLASERMVFPLSLRHANSYATKRVVLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVIA 353
KAIRLASERMVFPLSLRHANSYATKRVVLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVIA
Sbjct: 1337 KAIRLASERMVFPLSLRHANSYATKRVVLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVIA 1516
Query: 354 EGIALGSDIGEVNLLKKYEQERKPANVTMMAILDGFQKAYSIDFGPFNFLRGAAFSGANF 413
EGIALGSDIGEVNLLKKYEQERKPANVTMMAILDGFQKAYSIDFGPFNFLRGAAF GANF
Sbjct: 1517 EGIALGSDIGEVNLLKKYEQERKPANVTMMAILDGFQKAYSIDFGPFNFLRGAAFRGANF 1696
Query: 414 VSPLKRSIISYASGERKLPIFF 435
VSPLKRSIISYASGERKLPIFF
Sbjct: 1697 VSPLKRSIISYASGERKLPIFF 1762
Score = 57.4 bits (137), Expect(3) = 0.0
Identities = 25/27 (92%), Positives = 26/27 (95%)
Frame = +1
Query: 207 QRFLPNGPIALLPMGENFSNIVWTMSP 233
+RFLPNGPIALLPMGENFS IVWTMSP
Sbjct: 1075 KRFLPNGPIALLPMGENFSTIVWTMSP 1155
>BQ139044 similar to GP|9857294|dbj CpABA1 {Vigna unguiculata}, partial (35%)
Length = 668
Score = 47.8 bits (112), Expect = 8e-06
Identities = 37/126 (29%), Positives = 56/126 (44%), Gaps = 17/126 (13%)
Frame = +3
Query: 319 RVVLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVI----AEGIALGSDIGEVNLLKKYEQE 374
RV L+GD+ H + P GQG + D+Y L++ + + I G+ I + L+ YE E
Sbjct: 192 RVTLLGDSVHAMQPNMGQGGCMAIEDSYQLAKELDNAWEQSIKSGNPIKVDSALRSYESE 371
Query: 375 RK------PANVTMMAILDGFQKAY-SIDFGPFNFLRG------AAFSGANFVSPLKRSI 421
RK M A++ KAY + GP FL G FV L S+
Sbjct: 372 RKLRVAVIHGMARMAALMASTYKAYLGVGLGPLEFLTNFRIPHPGRVGGRFFVDILMPSM 551
Query: 422 ISYASG 427
+++ G
Sbjct: 552 LNWILG 569
>BI271514 similar to GP|9857294|dbj CpABA1 {Vigna unguiculata}, partial (30%)
Length = 653
Score = 47.4 bits (111), Expect = 1e-05
Identities = 32/96 (33%), Positives = 45/96 (46%), Gaps = 11/96 (11%)
Frame = +2
Query: 319 RVVLIGDAAHTIHPLAGQG----VNLGFGDAYSLSRVIAEGIALGSDIGEVNLLKKYEQE 374
RV L+GD+ H + P GQG + G+ A+ L + GS I + LK YE+E
Sbjct: 131 RVTLLGDSVHAMQPNMGQGGCMAIEDGYQLAFELDNAWQQSAKSGSTIDIASSLKSYERE 310
Query: 375 RK------PANVTMMAILDGFQKAY-SIDFGPFNFL 403
R+ M A++ KAY + GPF FL
Sbjct: 311 RRLRVTFVHGMARMAALMASTYKAYLGVGLGPFEFL 418
>TC91982 weakly similar to GP|170525|gb|AAA34202.1|| phenol hydroxylase
{Trichosporon cutaneum}, partial (15%)
Length = 1328
Score = 39.3 bits (90), Expect = 0.003
Identities = 29/92 (31%), Positives = 44/92 (47%), Gaps = 1/92 (1%)
Frame = +2
Query: 304 VFPLSLRHANSYATK-RVVLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVIAEGIALGSDI 362
V+ + R A+ + RV L GDA HT P AGQG+N+ D Y++ +A A+
Sbjct: 155 VYAIGQRLADRFTQDYRVFLTGDACHTHSPKAGQGMNVSLQDGYNIGWKLA---AVLCGQ 325
Query: 363 GEVNLLKKYEQERKPANVTMMAILDGFQKAYS 394
LL+ Y ER+ ++ F K +S
Sbjct: 326 ARPALLETYVTERQETAKDLIEFDRYFTKLFS 421
>TC80583 squalene monooxygenase 1 [Medicago truncatula]
Length = 1830
Score = 36.6 bits (83), Expect = 0.019
Identities = 29/123 (23%), Positives = 46/123 (36%), Gaps = 1/123 (0%)
Frame = +3
Query: 312 ANSYATKRVVLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVIAEGIALGSDIGEVNLLKKY 371
A+ Y T +L+GDA + HPL G G+ + D L ++ L L+ +
Sbjct: 1098 ADPYPTPGALLMGDAFNMRHPLTGGGMTVALSDIVVLRNLLKPLRDLNDASSLCKYLESF 1277
Query: 372 EQERKPANVTMMAILDGFQKAYSIDFGPF-NFLRGAAFSGANFVSPLKRSIISYASGERK 430
RKP T+ + K + P +R A F + +S SG
Sbjct: 1278 YTLRKPVASTINTLAGALYKVFCASPDPARKEMRQACFDYLSLGGLFSEGPVSLLSGLNP 1457
Query: 431 LPI 433
P+
Sbjct: 1458 CPL 1466
>TC79960 similar to PIR|T52419|T52419 CTF2A protein [imported] - Arabidopsis
thaliana, partial (53%)
Length = 907
Score = 34.7 bits (78), Expect = 0.074
Identities = 19/41 (46%), Positives = 25/41 (60%)
Frame = +1
Query: 140 EPSPAQGHASKLELSDGSSIYAKLVVGADGGKSRVRELAGF 180
EPSP + LE DGS I AK+V+G DG +S + + GF
Sbjct: 568 EPSP--NGDTFLEFLDGSKILAKIVIGCDGIRSPIAKWMGF 684
>TC76999 homologue to GP|19749359|gb|AAD28640.2 geranylgeranyl hydrogenase
{Glycine max}, complete
Length = 2228
Score = 34.7 bits (78), Expect = 0.074
Identities = 21/75 (28%), Positives = 34/75 (45%)
Frame = +2
Query: 319 RVVLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVIAEGIALGSDIGEVNLLKKYEQERKPA 378
RV L+GDAA + +G+G+ + I +G A G + E L+KY ++
Sbjct: 983 RVALVGDAAGYVTKCSGEGIYFAAKSGRMCAEAIVQGSANGKRMVEEGDLRKYLEKWDKT 1162
Query: 379 NVTMMAILDGFQKAY 393
+LD QK +
Sbjct: 1163 YWPTYKVLDILQKVF 1207
>TC88941 similar to PIR|T52419|T52419 CTF2A protein [imported] - Arabidopsis
thaliana, partial (39%)
Length = 659
Score = 34.3 bits (77), Expect = 0.097
Identities = 18/41 (43%), Positives = 25/41 (60%)
Frame = +1
Query: 140 EPSPAQGHASKLELSDGSSIYAKLVVGADGGKSRVRELAGF 180
EPSP + LE DGS + AK+V+G DG +S + + GF
Sbjct: 520 EPSP--NGDTLLEFLDGSKLVAKIVIGCDGIRSPIAKWMGF 636
>TC77092 squalene monooxygenase 2 [Medicago truncatula]
Length = 1910
Score = 33.1 bits (74), Expect = 0.22
Identities = 22/83 (26%), Positives = 34/83 (40%)
Frame = +3
Query: 312 ANSYATKRVVLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVIAEGIALGSDIGEVNLLKKY 371
A+ T VL+GDA + HPL G G+ + D L ++ L L+ +
Sbjct: 1122 ADPRPTPGAVLMGDAFNMRHPLTGGGMTVALSDIVVLRNLLKPMRDLNDAPTLCKYLESF 1301
Query: 372 EQERKPANVTMMAILDGFQKAYS 394
RKP T+ + K +S
Sbjct: 1302 YTLRKPVASTINTLAGALYKVFS 1370
>TC82836 similar to PIR|T05682|T05682 monooxygenase (EC 1.-.-.-) 2 -
Arabidopsis thaliana, partial (19%)
Length = 996
Score = 33.1 bits (74), Expect = 0.22
Identities = 26/98 (26%), Positives = 42/98 (42%), Gaps = 3/98 (3%)
Frame = +1
Query: 151 LELSDGSSIYAKLVVGADGGKSRVRELAGFKT---TGWNYSQNAIICTVEHTSANHCAWQ 207
L L+DG++I K+++G DG S V + GFK TG ++ C H Q
Sbjct: 400 LHLADGTTIKTKVLIGCDGVNSLVAKWLGFKEASYTGRYAARGYSECETNH-ELEPMFMQ 576
Query: 208 RFLPNGPIALLPMGENFSNIVWTMSPTESNDRKSITEE 245
F LP E + W + T ++ K + ++
Sbjct: 577 YFGKGFRAGALPCNE--KGVYWFFTWTPTSQEKELLQD 684
>TC86756 weakly similar to GP|18253083|dbj|BAB83948. CIG1 {Nicotiana
tabacum}, partial (71%)
Length = 1715
Score = 31.2 bits (69), Expect = 0.82
Identities = 18/57 (31%), Positives = 29/57 (50%)
Frame = +2
Query: 105 IKDSDFKTTFYPSRLTSMTLNTNSMSVVEENTPSKEPSPAQGHASKLELSDGSSIYA 161
IK+ FKTT P + + S++E PS P P+ S L+L+DG +++
Sbjct: 131 IKNLRFKTTTKPLNSSHPSATAAVASLLEREQPSP-PQPSHQQPSYLDLNDGERLFS 298
>TC88192 weakly similar to PIR|T05682|T05682 monooxygenase (EC 1.-.-.-) 2 -
Arabidopsis thaliana, partial (61%)
Length = 1557
Score = 30.8 bits (68), Expect = 1.1
Identities = 25/81 (30%), Positives = 35/81 (42%), Gaps = 10/81 (12%)
Frame = +3
Query: 306 PLSLRHA-----NSYATKRVVLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVIAEGIALGS 360
PL RH + + V + GDA H + P GQG D L+R +AE ++
Sbjct: 906 PLRYRHPWELIMGNISKGNVCVAGDALHPMTPDLGQGGCSALEDGVVLARCLAEALSKKQ 1085
Query: 361 DIGE-----VNLLKKYEQERK 376
E LKKY +ER+
Sbjct: 1086KEEEKYKRIEQGLKKYAKERR 1148
Score = 30.0 bits (66), Expect = 1.8
Identities = 14/35 (40%), Positives = 21/35 (60%)
Frame = +2
Query: 151 LELSDGSSIYAKLVVGADGGKSRVRELAGFKTTGW 185
L L DG++I K+++G DG S V + GFK +
Sbjct: 518 LHLVDGTTIKTKVLIGCDGINSVVAKWLGFKEASY 622
>BM813592 similar to GP|20161555|db putative homeodomain-leucine zipper
{Oryza sativa (japonica cultivar-group)}, partial (42%)
Length = 683
Score = 29.6 bits (65), Expect = 2.4
Identities = 17/57 (29%), Positives = 26/57 (44%)
Frame = -3
Query: 58 RHAYFKKMQVWDYTGFGYARYDAGDVNKDFLGCVAENKVLHSSLLSCIKDSDFKTTF 114
+H++ +VW + R+D V + FL V+ +L SS S I S T F
Sbjct: 273 KHSFCFGFKVWFFLKLSSRRWDCSFVRRSFLRGVSPLTLLSSSSSSAIHSSSMVTRF 103
>BF004918 similar to GP|21553624|gb unknown {Arabidopsis thaliana}, partial
(49%)
Length = 646
Score = 28.5 bits (62), Expect = 5.3
Identities = 20/93 (21%), Positives = 39/93 (41%)
Frame = +1
Query: 114 FYPSRLTSMTLNTNSMSVVEENTPSKEPSPAQGHASKLELSDGSSIYAKLVVGADGGKSR 173
F+P+ +++TL+ + + + S EP P H+S S +Y ++ + S
Sbjct: 154 FHPTLTSTVTLHLHRWIFIASSV-SLEPPPTPPHSSPSSCRRDSDMYNVAILFCNSHSSG 330
Query: 174 VRELAGFKTTGWNYSQNAIICTVEHTSANHCAW 206
+E +G + YS+ T + N W
Sbjct: 331 FQESSGIT*KAYGYSKRG---TS*YNGCNSIIW 420
>TC86353 similar to GP|15450733|gb|AAK96638.1 At2g36220/F2H17.17
{Arabidopsis thaliana}, partial (12%)
Length = 1351
Score = 28.5 bits (62), Expect = 5.3
Identities = 33/136 (24%), Positives = 59/136 (43%), Gaps = 3/136 (2%)
Frame = +1
Query: 14 SNPALSSGLCIKKEDPPDPRVSTVTPASISFLQEAGAWKYVEQNRHAYFKKMQVWDYTGF 73
S+P +S C+ K + STVT A+ + ++E A +Q++H++FK +
Sbjct: 511 SSPKVS---CMGKVRSKRGKTSTVTAAAKAAVKEKTARTERKQSKHSFFKSFR------- 660
Query: 74 GYARYDAGDVNKDFLGCVAENKVLHSSLLSCIKDSDFKTTFYPSRLTSMTLNTNSMSVVE 133
A + +G NK +++ SS L + + S+ T + N +S VE
Sbjct: 661 --AIFRSGGRNK-------HDRMNDSSALDSSSITKNTNSVSDSKARDSTASLNDVSFVE 813
Query: 134 ---ENTPSKEPSPAQG 146
N+ S+ P G
Sbjct: 814 SVTRNSVSEGEPPGLG 861
>TC76738
Length = 368
Score = 27.7 bits (60), Expect = 9.0
Identities = 15/70 (21%), Positives = 31/70 (43%)
Frame = -3
Query: 163 LVVGADGGKSRVRELAGFKTTGWNYSQNAIICTVEHTSANHCAWQRFLPNGPIALLPMGE 222
LV ++ G++++ L+GF + N + + N +W+ +P+G
Sbjct: 276 LVTASEEGQAQILNLSGFTRSSCNLKKRFLRFWSAQVLLNEASWRVIIPSG*SFAFYDTL 97
Query: 223 NFSNIVWTMS 232
+ S +VW S
Sbjct: 96 SKSQVVWDCS 67
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.317 0.133 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,925,426
Number of Sequences: 36976
Number of extensions: 177232
Number of successful extensions: 807
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 802
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 806
length of query: 435
length of database: 9,014,727
effective HSP length: 99
effective length of query: 336
effective length of database: 5,354,103
effective search space: 1798978608
effective search space used: 1798978608
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 60 (27.7 bits)
Medicago: description of AC121242.7