BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121242.1 + phase: 0 /pseudo
(403 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG644741 84 1e-16
BG580885 64 1e-10
AL366605 47 1e-05
BE999414 38 0.006
TC87382 similar to EGAD|146423|156195 vitellogenin {Anolis pulch... 31 0.98
TC80460 similar to GP|21537277|gb|AAM61618.1 unknown {Arabidopsi... 30 1.7
TC89175 GP|9087331|dbj|BAA99475.1 orf190 {Beta vulgaris}, partia... 30 2.2
BG449708 similar to GP|14194111|gb At1g15730/F7H2_7 {Arabidopsis... 29 2.8
TC77948 similar to PIR|C85360|C85360 hypothetical protein AT4g30... 24 3.1
TC88410 weakly similar to GP|12056928|gb|AAG48132.1 putative res... 28 4.8
TC81695 similar to GP|21539549|gb|AAM53327.1 putative guanine nu... 28 6.3
TC89918 similar to SP|Q39817|CALX_SOYBN Calnexin homolog precurs... 28 8.3
BF639281 similar to PIR|G71415|G71 hypothetical protein - Arabid... 28 8.3
>BG644741
Length = 735
Score = 83.6 bits (205), Expect = 1e-16
Identities = 40/109 (36%), Positives = 58/109 (52%)
Frame = -2
Query: 77 LHLFPFSLRDRASAWFHSLEVGSITSWDDMRRAFLARFFPPSKTAKLRDQITRFNQKDGE 136
L +FP SL A WF L SI +W+ +R FLAR++P SK D++ F GE
Sbjct: 587 LRVFPLSLMGEADIWFTELPYNSIFTWNQLRDVFLARYYPVSKKLNHNDRVNNFVALPGE 408
Query: 137 SLYEAWERFKEMLRLCPHHGLEKWLIVHTFYNGLSYTTKMSVDAAAGGA 185
S+ +W+RF LR P+H ++ + FY G K+ +D AGG+
Sbjct: 407 SVSSSWDRFTSFLRSVPNHRIDDDSLKEYFYRGQDDNNKVVLDTIAGGS 261
>BG580885
Length = 609
Score = 63.5 bits (153), Expect = 1e-10
Identities = 35/127 (27%), Positives = 68/127 (52%), Gaps = 2/127 (1%)
Frame = +3
Query: 46 FSGSPTEDPNLHISSFLRLSGTIKENQEAVRLHLFPFSLRDRASAWFH-SLEVGSIT-SW 103
F G+P E P H+S F ++ + ++ +FP +L + ++ W+ ++E I+ SW
Sbjct: 54 FRGTPNESPITHLSRFNKVCRANNASSVEMQKKIFPVTLEEESALWYDLNIEPYYISLSW 233
Query: 104 DDMRRAFLARFFPPSKTAKLRDQITRFNQKDGESLYEAWERFKEMLRLCPHHGLEKWLIV 163
D+++ +FL ++ +LR ++ +Q + E + + R + +L+ P HGLE +I
Sbjct: 234 DEIKLSFLQAYYEIEPVEELRSELMGIHQGEKERVRSYFLRLQWILKRWPEHGLEDDVIK 413
Query: 164 HTFYNGL 170
F NGL
Sbjct: 414 GVFVNGL 434
>AL366605
Length = 422
Score = 47.0 bits (110), Expect = 1e-05
Identities = 26/93 (27%), Positives = 50/93 (52%)
Frame = -3
Query: 54 PNLHISSFLRLSGTIKENQEAVRLHLFPFSLRDRASAWFHSLEVGSITSWDDMRRAFLAR 113
P HI ++R G K+N +++ +H F SL + A+ W+ SL I ++D++ AF +
Sbjct: 291 PQNHIIKYVRKMGNYKDN-DSLMIHCFQDSLMEDAAEWYTSLSKNDIHTFDELAAAFKSH 115
Query: 114 FFPPSKTAKLRDQITRFNQKDGESLYEAWERFK 146
+ ++ R+ + +QK ES E +R++
Sbjct: 114 YGFNTRLKPNREFLRSLSQKKEESFREYAQRWR 16
>BE999414
Length = 613
Score = 38.1 bits (87), Expect = 0.006
Identities = 24/73 (32%), Positives = 37/73 (49%)
Frame = +3
Query: 42 QQNQFSGSPTEDPNLHISSFLRLSGTIKENQEAVRLHLFPFSLRDRASAWFHSLEVGSIT 101
Q + + G T DP+ H+ + + + T + + AV+ LF +LR A WF +L SI
Sbjct: 6 QMDSYDG--T*DPDEHMEN-IEVVLTYRSVRGAVKCKLFVTTLRRGAMTWFKNLRRNSIG 176
Query: 102 SWDDMRRAFLARF 114
SW D+ F F
Sbjct: 177 SWGDLCHEFTTHF 215
>TC87382 similar to EGAD|146423|156195 vitellogenin {Anolis pulchellus},
partial (7%)
Length = 2304
Score = 30.8 bits (68), Expect = 0.98
Identities = 21/81 (25%), Positives = 34/81 (41%), Gaps = 13/81 (16%)
Frame = +2
Query: 50 PTEDPNLHISSFLRLSGTI------KENQEAVRLHLFPFSLRDRASAWFHSLE------- 96
P + NL + FL TI KE E ++ + L+ A W+ +L+
Sbjct: 629 PDFEGNLQLDDFLDWLQTIERVFEYKEVPEEQKVKIVAAKLKKHALIWWENLKRRRKREG 808
Query: 97 VGSITSWDDMRRAFLARFFPP 117
I +WD MR+ ++ PP
Sbjct: 809 KSKIKTWDKMRQKLTRKYLPP 871
>TC80460 similar to GP|21537277|gb|AAM61618.1 unknown {Arabidopsis
thaliana}, partial (52%)
Length = 1867
Score = 30.0 bits (66), Expect = 1.7
Identities = 14/30 (46%), Positives = 19/30 (62%)
Frame = -1
Query: 334 LENCIANQNKNLQELKNQTGFLNDSLSPHT 363
++NC+ NKNLQEL+N + S PHT
Sbjct: 358 MQNCLNQ*NKNLQELQNAEQRILHS*LPHT 269
>TC89175 GP|9087331|dbj|BAA99475.1 orf190 {Beta vulgaris}, partial (5%)
Length = 648
Score = 29.6 bits (65), Expect = 2.2
Identities = 18/57 (31%), Positives = 27/57 (46%)
Frame = +3
Query: 336 NCIANQNKNLQELKNQTGFLNDSLSPHTPKCLRPKSPKWPNKWPPLLKHQESFLVNL 392
NC+ NQ K+L + FL+ + P+ R KSP N LL QE +++
Sbjct: 78 NCLLNQTKSLYQFHLILSFLDKMILLLFPEHYRKKSPMNTNLLSDLLTLQERLTLSM 248
>BG449708 similar to GP|14194111|gb At1g15730/F7H2_7 {Arabidopsis thaliana},
partial (31%)
Length = 647
Score = 29.3 bits (64), Expect = 2.8
Identities = 20/53 (37%), Positives = 28/53 (52%), Gaps = 1/53 (1%)
Frame = +2
Query: 338 IANQNKNLQELKNQ-TGFLNDSLSPHTPKCLRPKSPKWPNKWPPLLKHQESFL 389
I N+ +LQ L++ T + +SL+ H P LR K P P PPL +FL
Sbjct: 98 IFNR*THLQFLQSSLTTTITNSLASHHPPHLRLKPPMTPTTSPPLFHLITAFL 256
>TC77948 similar to PIR|C85360|C85360 hypothetical protein AT4g30780
[imported] - Arabidopsis thaliana, partial (28%)
Length = 1916
Score = 24.3 bits (51), Expect(2) = 3.1
Identities = 10/27 (37%), Positives = 16/27 (59%), Gaps = 1/27 (3%)
Frame = -2
Query: 142 WERFKEMLRLCPHHGLEK-WLIVHTFY 167
W + ++ L PHH ++K L +H FY
Sbjct: 1012 WRKMIDLQILIPHHAVQKSQLSLHFFY 932
Score = 23.1 bits (48), Expect(2) = 3.1
Identities = 14/43 (32%), Positives = 18/43 (41%)
Frame = -1
Query: 72 QEAVRLHLFPFSLRDRASAWFHSLEVGSITSWDDMRRAFLARF 114
Q A +L F S + AW E+G + SW A RF
Sbjct: 1151 QIAEKLIRFRSSAPETFDAWGFITELGEVISWKLFSTALPIRF 1023
>TC88410 weakly similar to GP|12056928|gb|AAG48132.1 putative resistance
protein {Glycine max}, partial (8%)
Length = 2252
Score = 28.5 bits (62), Expect = 4.8
Identities = 11/33 (33%), Positives = 19/33 (57%)
Frame = +3
Query: 344 NLQELKNQTGFLNDSLSPHTPKCLRPKSPKWPN 376
N+ ++KNQTG +L+ H + + + KW N
Sbjct: 456 NISDVKNQTGSYGQALAVHKNRVMPERFEKWIN 554
>TC81695 similar to GP|21539549|gb|AAM53327.1 putative guanine nucleotide
regulatory protein {Arabidopsis thaliana}, partial (22%)
Length = 778
Score = 28.1 bits (61), Expect = 6.3
Identities = 14/26 (53%), Positives = 17/26 (64%)
Frame = -1
Query: 321 NQRVAQKSSLEILLENCIANQNKNLQ 346
N RV K+SL +NCI Q+KNLQ
Sbjct: 220 NYRVDVKNSLVPSSKNCIVQQSKNLQ 143
>TC89918 similar to SP|Q39817|CALX_SOYBN Calnexin homolog precursor. [Soybean]
{Glycine max}, partial (27%)
Length = 1064
Score = 27.7 bits (60), Expect = 8.3
Identities = 14/42 (33%), Positives = 19/42 (44%)
Frame = -3
Query: 334 LENCIANQNKNLQELKNQTGFLNDSLSPHTPKCLRPKSPKWP 375
+EN I N K + E K+ + D + T K R P WP
Sbjct: 1035 IENMITNG*KQIHEEKSSRKLMGDDPNTRTQKKQRRFQPLWP 910
>BF639281 similar to PIR|G71415|G71 hypothetical protein - Arabidopsis
thaliana, partial (0%)
Length = 482
Score = 27.7 bits (60), Expect = 8.3
Identities = 18/56 (32%), Positives = 28/56 (49%)
Frame = +1
Query: 275 DCQLGSAANIEQLNYAQYNQGMRPNQNFYKNPQGSYGQAAPPGYTNNQRVAQKSSL 330
D +L S A EQ + NQG R N + ++P + G+ +N A+KSS+
Sbjct: 322 DPKLRSPARTEQ---SPRNQGWRHNDHKLRSPARTEQSPRGQGWRHNDHKAEKSSM 480
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.315 0.131 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,485,071
Number of Sequences: 36976
Number of extensions: 201116
Number of successful extensions: 1134
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 1114
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1132
length of query: 403
length of database: 9,014,727
effective HSP length: 98
effective length of query: 305
effective length of database: 5,391,079
effective search space: 1644279095
effective search space used: 1644279095
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 59 (27.3 bits)
Medicago: description of AC121242.1