BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121239.10 - phase: 0
(90 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC82871 similar to GP|10177227|dbj|BAB10601. alanyl-tRNA synthet... 183 9e-48
BQ151242 similar to PIR|G86292|G86 hypothetical protein AAF82153... 32 0.042
TC81266 weakly similar to GP|16415994|emb|CAB88653. hypothetical... 29 0.36
TC82284 weakly similar to GP|11119510|gb|AAF37724.2 diaphanous p... 29 0.36
TC81144 similar to PIR|T04462|T04462 hypothetical protein F4D11.... 28 0.46
BQ143793 weakly similar to GP|6523547|emb| hydroxyproline-rich g... 27 1.0
TC85960 similar to GP|23094138|gb|AAF50778.2 CG4835-PA {Drosophi... 27 1.4
TC77953 similar to PIR|D84890|D84890 probable AT-hook DNA-bindin... 27 1.4
BQ144303 weakly similar to GP|6523547|emb| hydroxyproline-rich g... 27 1.8
BF006609 similar to GP|21955291|gb|A unknown protein {Arabidopsi... 27 1.8
TC87483 similar to GP|21955291|gb|AAK59492.2 unknown protein {Ar... 27 1.8
AW585061 similar to GP|15553336|gb| calyx protein; polyhedron en... 26 2.3
BE325479 SP|Q07176|MMK1 Mitogen-activated protein kinase homolog... 26 2.3
TC82309 similar to PIR|B84420|B84420 transfactor-like protein [i... 26 3.0
TC93249 weakly similar to GP|13877617|gb|AAK43886.1 protein kina... 26 3.0
TC80017 similar to GP|10177301|dbj|BAB10562. gene_id:MDC12.17~un... 26 3.0
BQ144486 similar to GP|12836661|db H6 homeo box 3~data source:MG... 26 3.0
BQ750920 similar to GP|15811426|gb| glycine-rich protein {Coccid... 26 3.0
TC93591 similar to PIR|T05209|T05209 hypothetical protein F24J7.... 26 3.0
BQ144482 similar to GP|6523547|emb| hydroxyproline-rich glycopro... 25 3.9
>TC82871 similar to GP|10177227|dbj|BAB10601. alanyl-tRNA synthetase
{Arabidopsis thaliana}, partial (11%)
Length = 689
Score = 183 bits (465), Expect = 9e-48
Identities = 90/90 (100%), Positives = 90/90 (100%)
Frame = +2
Query: 1 METLADPAAIVLGSCPGEGKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGGRPNFA 60
METLADPAAIVLGSCPGEGKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGGRPNFA
Sbjct: 65 METLADPAAIVLGSCPGEGKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGGRPNFA 244
Query: 61 QAGGRKPENLAGALEKAKSELIAYLCEKGN 90
QAGGRKPENLAGALEKAKSELIAYLCEKGN
Sbjct: 245 QAGGRKPENLAGALEKAKSELIAYLCEKGN 334
>BQ151242 similar to PIR|G86292|G86 hypothetical protein AAF82153.1
[imported] - Arabidopsis thaliana, partial (5%)
Length = 1371
Score = 32.0 bits (71), Expect = 0.042
Identities = 15/29 (51%), Positives = 16/29 (54%)
Frame = +2
Query: 50 GGGGGGRPNFAQAGGRKPENLAGALEKAK 78
GGGGGG Q G RK E GA E+ K
Sbjct: 167 GGGGGGERGGGQKGRRKEEGAEGAAERPK 253
>TC81266 weakly similar to GP|16415994|emb|CAB88653. hypothetical protein
{Neurospora crassa}, partial (2%)
Length = 644
Score = 28.9 bits (63), Expect = 0.36
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = -2
Query: 45 IAKLCGGGGGGRPNFAQAGGRK 66
+ + CGGGGGG F AGG K
Sbjct: 364 LGRCCGGGGGGAIGF*LAGGGK 299
>TC82284 weakly similar to GP|11119510|gb|AAF37724.2 diaphanous protein
{Entamoeba histolytica}, partial (4%)
Length = 671
Score = 28.9 bits (63), Expect = 0.36
Identities = 19/56 (33%), Positives = 23/56 (40%), Gaps = 4/56 (7%)
Frame = -1
Query: 13 GSCPGEGKVSLVAA---FTPGVVGQGIQAGKFIGQIA-KLCGGGGGGRPNFAQAGG 64
G PG G + + P + G G + G IG CGGGGGG GG
Sbjct: 233 GIAPGLGPFAAIGGGGGIDPDIGGGGARGGLPIGAPGGSPCGGGGGGGSGACGGGG 66
>TC81144 similar to PIR|T04462|T04462 hypothetical protein F4D11.160 -
Arabidopsis thaliana, partial (13%)
Length = 1102
Score = 28.5 bits (62), Expect = 0.46
Identities = 15/42 (35%), Positives = 19/42 (44%)
Frame = -3
Query: 35 GIQAGKFIGQIAKLCGGGGGGRPNFAQAGGRKPENLAGALEK 76
G G +G+ K CGGG GR GG P GA ++
Sbjct: 290 GALKGL*LGRAPKFCGGGPDGRRGGEPPGGGTPTPETGAAKE 165
>BQ143793 weakly similar to GP|6523547|emb| hydroxyproline-rich glycoprotein
DZ-HRGP {Volvox carteri f. nagariensis}, partial (19%)
Length = 1100
Score = 27.3 bits (59), Expect = 1.0
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = -1
Query: 33 GQGIQAGKFIGQIAKLCGGGGGGRPNFAQAGGR 65
G GI G++ G GGGGGG+ + GGR
Sbjct: 389 GNGIYEGEYKG------GGGGGGKRKKKREGGR 309
>TC85960 similar to GP|23094138|gb|AAF50778.2 CG4835-PA {Drosophila
melanogaster}, partial (2%)
Length = 1834
Score = 26.9 bits (58), Expect = 1.4
Identities = 12/20 (60%), Positives = 12/20 (60%)
Frame = -3
Query: 49 CGGGGGGRPNFAQAGGRKPE 68
C GG G NFA GGR PE
Sbjct: 1031 CSRGGRGALNFACFGGRPPE 972
>TC77953 similar to PIR|D84890|D84890 probable AT-hook DNA-binding protein
[imported] - Arabidopsis thaliana, partial (57%)
Length = 1695
Score = 26.9 bits (58), Expect = 1.4
Identities = 11/24 (45%), Positives = 16/24 (65%)
Frame = +1
Query: 45 IAKLCGGGGGGRPNFAQAGGRKPE 68
I +L GGGGGG P+ GG++ +
Sbjct: 1180 IQQLQGGGGGGSPSGGGGGGQQQQ 1251
>BQ144303 weakly similar to GP|6523547|emb| hydroxyproline-rich glycoprotein
DZ-HRGP {Volvox carteri f. nagariensis}, partial (11%)
Length = 1158
Score = 26.6 bits (57), Expect = 1.8
Identities = 14/27 (51%), Positives = 16/27 (58%)
Frame = +1
Query: 50 GGGGGGRPNFAQAGGRKPENLAGALEK 76
GGGGGGR + + GG K GA EK
Sbjct: 448 GGGGGGRRIYGERGGGK-----GAREK 513
>BF006609 similar to GP|21955291|gb|A unknown protein {Arabidopsis thaliana},
partial (97%)
Length = 597
Score = 26.6 bits (57), Expect = 1.8
Identities = 19/56 (33%), Positives = 29/56 (50%), Gaps = 2/56 (3%)
Frame = +1
Query: 4 LADPAAIVLGSCPGEGKVS-LVAAFTPGVVGQGIQAGKFIGQIA-KLCGGGGGGRP 57
+A P+ SC G G+V L + ++ G +G +G G++ CGG G GRP
Sbjct: 151 MAGPSFPRCPSCNGTGRVPCLCSRWSDGDIGCSTCSGS--GRMGCSSCGGSGTGRP 312
>TC87483 similar to GP|21955291|gb|AAK59492.2 unknown protein {Arabidopsis
thaliana}, partial (97%)
Length = 679
Score = 26.6 bits (57), Expect = 1.8
Identities = 19/56 (33%), Positives = 29/56 (50%), Gaps = 2/56 (3%)
Frame = +3
Query: 4 LADPAAIVLGSCPGEGKVS-LVAAFTPGVVGQGIQAGKFIGQIA-KLCGGGGGGRP 57
+A P+ SC G G+V L + ++ G +G +G G++ CGG G GRP
Sbjct: 225 MAGPSFPRCPSCNGTGRVPCLCSRWSDGDIGCSTCSGS--GRMGCSSCGGSGTGRP 386
>AW585061 similar to GP|15553336|gb| calyx protein; polyhedron envelope
protein PEP {Spodoptera litura nucleopolyhedrovirus},
partial (6%)
Length = 483
Score = 26.2 bits (56), Expect = 2.3
Identities = 14/29 (48%), Positives = 15/29 (51%)
Frame = +1
Query: 50 GGGGGGRPNFAQAGGRKPENLAGALEKAK 78
GGGGGGR Q G RK G E A+
Sbjct: 76 GGGGGGRVVKKQRGARKAGKGRGDTEGAR 162
>BE325479 SP|Q07176|MMK1 Mitogen-activated protein kinase homolog MMK1 (EC
2.7.1.-) (MAP kinase MSK7) (MAP kinase ERK1)., partial
(32%)
Length = 557
Score = 26.2 bits (56), Expect = 2.3
Identities = 13/38 (34%), Positives = 18/38 (47%)
Frame = -3
Query: 48 LCGGGGGGRPNFAQAGGRKPENLAGALEKAKSELIAYL 85
+CGG G AGG P ++ +L +A LI L
Sbjct: 243 ICGGAGAASDITVSAGGAPPPSIVVSLLQALKNLIFIL 130
>TC82309 similar to PIR|B84420|B84420 transfactor-like protein [imported] -
Arabidopsis thaliana, partial (44%)
Length = 954
Score = 25.8 bits (55), Expect = 3.0
Identities = 10/15 (66%), Positives = 10/15 (66%)
Frame = +1
Query: 50 GGGGGGRPNFAQAGG 64
GGGGGG FA GG
Sbjct: 100 GGGGGGGEQFANGGG 144
>TC93249 weakly similar to GP|13877617|gb|AAK43886.1 protein kinase-like
protein {Arabidopsis thaliana}, partial (5%)
Length = 669
Score = 25.8 bits (55), Expect = 3.0
Identities = 12/27 (44%), Positives = 16/27 (58%)
Frame = -3
Query: 33 GQGIQAGKFIGQIAKLCGGGGGGRPNF 59
G G + G+ +G + GGGGGG NF
Sbjct: 193 GSGAENGEDVGDVVG-DGGGGGGLENF 116
>TC80017 similar to GP|10177301|dbj|BAB10562. gene_id:MDC12.17~unknown
protein {Arabidopsis thaliana}, partial (45%)
Length = 879
Score = 25.8 bits (55), Expect = 3.0
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = -1
Query: 41 FIGQIAKLCGGGGGGR 56
FI + +CGG GGGR
Sbjct: 177 FINLVISVCGGSGGGR 130
>BQ144486 similar to GP|12836661|db H6 homeo box 3~data source:MGD source
key:MGI:107160 evidence:ISS~putative {Mus musculus},
partial (1%)
Length = 1490
Score = 25.8 bits (55), Expect = 3.0
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = +1
Query: 50 GGGGGGRPNFAQAGGR 65
GGGGGG A+ GGR
Sbjct: 130 GGGGGGGSQMAEKGGR 177
>BQ750920 similar to GP|15811426|gb| glycine-rich protein {Coccidioides
immitis}, partial (18%)
Length = 729
Score = 25.8 bits (55), Expect = 3.0
Identities = 17/57 (29%), Positives = 26/57 (44%), Gaps = 7/57 (12%)
Frame = +1
Query: 28 TPGVVGQGIQAG-------KFIGQIAKLCGGGGGGRPNFAQAGGRKPENLAGALEKA 77
TP ++ Q +AG + G GGGGG R +++GGR +L + A
Sbjct: 343 TPPILTQRWRAGAG*SACLQLSGSGGNGSGGGGGRRGGSSRSGGRGRSSLGSGISGA 513
>TC93591 similar to PIR|T05209|T05209 hypothetical protein F24J7.50 -
Arabidopsis thaliana, partial (12%)
Length = 688
Score = 25.8 bits (55), Expect = 3.0
Identities = 19/50 (38%), Positives = 25/50 (50%), Gaps = 4/50 (8%)
Frame = -3
Query: 21 VSLVAAFTPGV--VGQGIQAGKFIGQIAKLCGGGGGGRPNFAQ--AGGRK 66
VS+ A F V +G+G + K IG G GGG R F + GGR+
Sbjct: 383 VSVFADFVEAVEGIGEGREVWKEIGSGESGGGFGGGDRGEFGRGDCGGRE 234
>BQ144482 similar to GP|6523547|emb| hydroxyproline-rich glycoprotein DZ-HRGP
{Volvox carteri f. nagariensis}, partial (16%)
Length = 993
Score = 25.4 bits (54), Expect = 3.9
Identities = 16/43 (37%), Positives = 21/43 (48%)
Frame = -1
Query: 30 GVVGQGIQAGKFIGQIAKLCGGGGGGRPNFAQAGGRKPENLAG 72
G G G + G+ GQ + GGGGGGR A+ G + G
Sbjct: 342 GARGAGGRGGEGRGQ-GEAEGGGGGGRGRGAKEGEGRERGRGG 217
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.315 0.139 0.410
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,215,336
Number of Sequences: 36976
Number of extensions: 25575
Number of successful extensions: 352
Number of sequences better than 10.0: 65
Number of HSP's better than 10.0 without gapping: 308
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 347
length of query: 90
length of database: 9,014,727
effective HSP length: 66
effective length of query: 24
effective length of database: 6,574,311
effective search space: 157783464
effective search space used: 157783464
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 51 (24.3 bits)
Medicago: description of AC121239.10