BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121238.7 - phase: 0
(83 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC87254 similar to PIR|JC7234|JC7234 27k vesicle-associated memb... 98 6e-22
AW775086 similar to PIR|JC7234|JC7 27k vesicle-associated membra... 90 2e-19
TC78518 similar to PIR|JC7234|JC7234 27k vesicle-associated memb... 89 2e-19
TC81745 similar to PIR|JC7234|JC7234 27k vesicle-associated memb... 79 3e-16
BG452644 similar to GP|21554053|gb putative VAMP-associated prot... 79 4e-16
TC90632 similar to GP|21554053|gb|AAM63134.1 putative VAMP-assoc... 79 4e-16
CA917079 weakly similar to PIR|B86220|B86 protein F22O13.31 [imp... 62 5e-11
BI266722 similar to GP|17473763|gb| putative protein {Arabidopsi... 40 2e-04
TC80263 similar to GP|20466540|gb|AAM20587.1 putative protein {A... 30 0.13
BQ144161 homologue to GP|17429361|emb PROBABLE CELL DIVISION FTS... 29 0.37
BQ137239 similar to GP|6523547|emb| hydroxyproline-rich glycopro... 28 0.48
BQ143931 similar to PIR|T02229|T022 protein BYJ15 - common tobac... 28 0.82
BF636819 27 1.4
TC89101 similar to GP|7268289|emb|CAB78584.1 UFD1 like protein {... 27 1.8
TC89715 similar to GP|12003384|gb|AAG43549.1 Avr9/Cf-9 rapidly e... 27 1.8
BQ141694 similar to GP|12697907|db KIAA1681 protein {Homo sapien... 27 1.8
CA921832 similar to GP|14194103|gb AT3g22390/MCB17_12 {Arabidops... 26 2.4
TC77868 similar to GP|11994207|dbj|BAB01310. gene_id:MPN9.20~unk... 26 3.1
TC88568 similar to GP|19310741|gb|AAL85101.1 unknown protein {Ar... 26 3.1
AA660403 25 4.1
>TC87254 similar to PIR|JC7234|JC7234 27k vesicle-associated membrane
protein-associated protein - curled-leaved tobacco,
partial (87%)
Length = 1040
Score = 97.8 bits (242), Expect = 6e-22
Identities = 49/78 (62%), Positives = 55/78 (69%)
Frame = +2
Query: 1 MQALEEAPPDMQCKDKFLLQSIVARPGATTKDITSDMFYKDSGYEVKESKFRAVCVAPPP 60
MQ +EAPPDMQCKDKFLLQS+VA PGAT KDIT +MF K+SGYEV E K R V VAPP
Sbjct: 275 MQGQKEAPPDMQCKDKFLLQSVVASPGATAKDITPEMFNKESGYEVDECKLRVVYVAPPQ 454
Query: 61 SPNPPLSVHASESLDQVS 78
P+P ES + S
Sbjct: 455 PPSPVREGSDEESSPRAS 508
>AW775086 similar to PIR|JC7234|JC7 27k vesicle-associated membrane
protein-associated protein - curled-leaved tobacco,
partial (52%)
Length = 747
Score = 89.7 bits (221), Expect = 2e-19
Identities = 45/72 (62%), Positives = 56/72 (77%)
Frame = +2
Query: 1 MQALEEAPPDMQCKDKFLLQSIVARPGATTKDITSDMFYKDSGYEVKESKFRAVCVAPPP 60
MQA ++APP+M C DKFL+QSIVA+PGATT+DITS+MF++DSGY+V+ K R V APP
Sbjct: 236 MQAQKKAPPNMHCIDKFLIQSIVAKPGATTEDITSEMFFQDSGYKVEMCKLRVVYDAPP- 412
Query: 61 SPNPPLSVHASE 72
PN P ASE
Sbjct: 413 -PNLPSPAQASE 445
>TC78518 similar to PIR|JC7234|JC7234 27k vesicle-associated membrane
protein-associated protein - curled-leaved tobacco,
partial (83%)
Length = 827
Score = 89.4 bits (220), Expect = 2e-19
Identities = 41/64 (64%), Positives = 50/64 (78%)
Frame = +1
Query: 1 MQALEEAPPDMQCKDKFLLQSIVARPGATTKDITSDMFYKDSGYEVKESKFRAVCVAPPP 60
MQA +EAP DMQCKDKFLLQS++A PG TTKDI +MF K++G+ V+E K R V VAPP
Sbjct: 292 MQAQKEAPSDMQCKDKFLLQSVIASPGTTTKDINPEMFSKEAGHNVEECKLRVVYVAPPG 471
Query: 61 SPNP 64
P+P
Sbjct: 472 PPSP 483
>TC81745 similar to PIR|JC7234|JC7234 27k vesicle-associated membrane
protein-associated protein - curled-leaved tobacco,
partial (94%)
Length = 945
Score = 79.0 bits (193), Expect = 3e-16
Identities = 36/64 (56%), Positives = 48/64 (74%)
Frame = +2
Query: 1 MQALEEAPPDMQCKDKFLLQSIVARPGATTKDITSDMFYKDSGYEVKESKFRAVCVAPPP 60
MQA +EAP DMQCKDKFLLQS+ G + KDI+++MF K++G+ V+E K R V V+PP
Sbjct: 314 MQAQKEAPADMQCKDKFLLQSVKTNDGVSPKDISAEMFNKEAGHVVEECKLRVVYVSPPQ 493
Query: 61 SPNP 64
P+P
Sbjct: 494 PPSP 505
>BG452644 similar to GP|21554053|gb putative VAMP-associated protein
{Arabidopsis thaliana}, partial (61%)
Length = 629
Score = 78.6 bits (192), Expect = 4e-16
Identities = 37/64 (57%), Positives = 46/64 (71%)
Frame = +2
Query: 1 MQALEEAPPDMQCKDKFLLQSIVARPGATTKDITSDMFYKDSGYEVKESKFRAVCVAPPP 60
MQA +E PPDMQCKDKFLLQS+ GA K+IT +MF K++G+ V+E K R V VAPP
Sbjct: 371 MQAQKEVPPDMQCKDKFLLQSVRVNDGADAKEITPEMFNKEAGHVVEECKLRVVYVAPPQ 550
Query: 61 SPNP 64
+P
Sbjct: 551 PQSP 562
>TC90632 similar to GP|21554053|gb|AAM63134.1 putative VAMP-associated
protein {Arabidopsis thaliana}, partial (63%)
Length = 681
Score = 78.6 bits (192), Expect = 4e-16
Identities = 37/64 (57%), Positives = 46/64 (71%)
Frame = +1
Query: 1 MQALEEAPPDMQCKDKFLLQSIVARPGATTKDITSDMFYKDSGYEVKESKFRAVCVAPPP 60
MQA +E PPDMQCKDKFLLQS+ GA K+IT +MF K++G+ V+E K R V VAPP
Sbjct: 379 MQAQKEVPPDMQCKDKFLLQSVRVNDGADAKEITPEMFNKEAGHVVEECKLRVVYVAPPQ 558
Query: 61 SPNP 64
+P
Sbjct: 559 PQSP 570
>CA917079 weakly similar to PIR|B86220|B86 protein F22O13.31 [imported] -
Arabidopsis thaliana, partial (23%)
Length = 487
Score = 61.6 bits (148), Expect = 5e-11
Identities = 28/62 (45%), Positives = 40/62 (64%)
Frame = +1
Query: 1 MQALEEAPPDMQCKDKFLLQSIVARPGATTKDITSDMFYKDSGYEVKESKFRAVCVAPPP 60
MQA AP D+ CKDKFL+QS + TT+D+TS +F KD ++E+K + ++PP
Sbjct: 100 MQAQRVAPEDLVCKDKFLVQSTLVDAEITTEDVTSSLFVKDGLRYIEENKLKVALISPPD 279
Query: 61 SP 62
SP
Sbjct: 280 SP 285
>BI266722 similar to GP|17473763|gb| putative protein {Arabidopsis thaliana},
partial (28%)
Length = 404
Score = 40.0 bits (92), Expect = 2e-04
Identities = 17/22 (77%), Positives = 19/22 (86%)
Frame = +2
Query: 1 MQALEEAPPDMQCKDKFLLQSI 22
MQA +E PPDMQCKDK LLQS+
Sbjct: 326 MQAQKEVPPDMQCKDKXLLQSV 391
>TC80263 similar to GP|20466540|gb|AAM20587.1 putative protein {Arabidopsis
thaliana}, partial (46%)
Length = 1041
Score = 30.4 bits (67), Expect = 0.13
Identities = 22/62 (35%), Positives = 28/62 (44%), Gaps = 5/62 (8%)
Frame = -3
Query: 26 PGATTKDITSDMFYKDSGYEVKESKFRAVCVAPPPSPNP-PL----SVHASESLDQVSFY 80
PGAT++ + D Y S + K CV P +P P PL S HAS + Y
Sbjct: 796 PGATSEGLNLDFSYNTSRIRLSFIKSLPYCVTPTDAPMPLPLNSLPSSHASILYPFETVY 617
Query: 81 FI 82
FI
Sbjct: 616 FI 611
>BQ144161 homologue to GP|17429361|emb PROBABLE CELL DIVISION FTSK
TRANSMEMBRANE PROTEIN {Ralstonia solanacearum}, partial
(1%)
Length = 1132
Score = 28.9 bits (63), Expect = 0.37
Identities = 9/16 (56%), Positives = 12/16 (74%)
Frame = -2
Query: 55 CVAPPPSPNPPLSVHA 70
C+APPP+P PP H+
Sbjct: 84 CIAPPPNPTPPPRFHS 37
>BQ137239 similar to GP|6523547|emb| hydroxyproline-rich glycoprotein DZ-HRGP
{Volvox carteri f. nagariensis}, partial (13%)
Length = 1097
Score = 28.5 bits (62), Expect = 0.48
Identities = 20/63 (31%), Positives = 29/63 (45%), Gaps = 2/63 (3%)
Frame = -2
Query: 21 SIVARPGA--TTKDITSDMFYKDSGYEVKESKFRAVCVAPPPSPNPPLSVHASESLDQVS 78
S+ RP A T + + +F+ S + S +C PPPSP+ P S A L +S
Sbjct: 364 SLPPRPPALPTPPPLLTSLFFLQSSFF---SGCGILCPCPPPSPSCPASPRALPPLS*IS 194
Query: 79 FYF 81
F
Sbjct: 193 LLF 185
>BQ143931 similar to PIR|T02229|T022 protein BYJ15 - common tobacco
(fragment), partial (15%)
Length = 1239
Score = 27.7 bits (60), Expect = 0.82
Identities = 10/16 (62%), Positives = 12/16 (74%)
Frame = +2
Query: 58 PPPSPNPPLSVHASES 73
PPP PNPPL + S+S
Sbjct: 5 PPPPPNPPLIIFISQS 52
>BF636819
Length = 635
Score = 26.9 bits (58), Expect = 1.4
Identities = 15/62 (24%), Positives = 26/62 (41%)
Frame = +2
Query: 18 LLQSIVARPGATTKDITSDMFYKDSGYEVKESKFRAVCVAPPPSPNPPLSVHASESLDQV 77
L+ ++ R G + F ++ + +K S + PPP P + V AS S +
Sbjct: 269 LVTDVMGRDGDDEYEYAEAEFAEEKKFGLKFSSSHWLMAPPPPEPPDKVKVDASVSSRNI 448
Query: 78 SF 79
F
Sbjct: 449 HF 454
>TC89101 similar to GP|7268289|emb|CAB78584.1 UFD1 like protein {Arabidopsis
thaliana}, partial (6%)
Length = 894
Score = 26.6 bits (57), Expect = 1.8
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +1
Query: 58 PPPSPNPPLSVHASESLDQVSF 79
PPP P PP S+H E++ + F
Sbjct: 403 PPPPPPPPPSLHEVEAILEYEF 468
>TC89715 similar to GP|12003384|gb|AAG43549.1 Avr9/Cf-9 rapidly elicited
protein 111B {Nicotiana tabacum}, partial (49%)
Length = 947
Score = 26.6 bits (57), Expect = 1.8
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = -3
Query: 56 VAPPPSPNPPLSVHA 70
VAP PSP PPLS A
Sbjct: 480 VAPSPSPTPPLSTKA 436
>BQ141694 similar to GP|12697907|db KIAA1681 protein {Homo sapiens}, partial
(2%)
Length = 1247
Score = 26.6 bits (57), Expect = 1.8
Identities = 11/24 (45%), Positives = 13/24 (53%)
Frame = +1
Query: 55 CVAPPPSPNPPLSVHASESLDQVS 78
CV P P+P PP H + QVS
Sbjct: 310 CVKPNPAPTPPTL*HGYVKVSQVS 381
>CA921832 similar to GP|14194103|gb AT3g22390/MCB17_12 {Arabidopsis
thaliana}, partial (3%)
Length = 802
Score = 26.2 bits (56), Expect = 2.4
Identities = 13/36 (36%), Positives = 17/36 (47%)
Frame = +3
Query: 38 FYKDSGYEVKESKFRAVCVAPPPSPNPPLSVHASES 73
F +D E E ++ PPP P PP S +S S
Sbjct: 207 FVRDEEEESVEGFNGSILTEPPPPPPPPSSSSSSSS 314
>TC77868 similar to GP|11994207|dbj|BAB01310. gene_id:MPN9.20~unknown
protein {Arabidopsis thaliana}, partial (36%)
Length = 1019
Score = 25.8 bits (55), Expect = 3.1
Identities = 9/27 (33%), Positives = 16/27 (58%)
Frame = -3
Query: 56 VAPPPSPNPPLSVHASESLDQVSFYFI 82
+ PPP P PP+ +H + L+ ++ I
Sbjct: 912 LXPPPLPPPPVLLHPTTDLNPITTVII 832
>TC88568 similar to GP|19310741|gb|AAL85101.1 unknown protein {Arabidopsis
thaliana}, partial (40%)
Length = 686
Score = 25.8 bits (55), Expect = 3.1
Identities = 14/53 (26%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
Frame = +1
Query: 17 FLLQSIVARPGATTKDITSDMFY-KDSGYEVKESKFRAVCVAPPPSPNPPLSV 68
F LQS+ + + T +I SDM+ +++ + + V PPP+ P ++
Sbjct: 145 FKLQSLSTKIVSATTNIASDMYIGRNNSSNKIYTNLNSCLVIPPPTSTKPRAI 303
>AA660403
Length = 283
Score = 25.4 bits (54), Expect = 4.1
Identities = 15/37 (40%), Positives = 17/37 (45%)
Frame = +3
Query: 26 PGATTKDITSDMFYKDSGYEVKESKFRAVCVAPPPSP 62
P T D TSD + + E S FR V PPP P
Sbjct: 57 PADTAFDFTSDPSFPNFAEEX--SSFRLVXGXPPPRP 161
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.318 0.133 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,874,460
Number of Sequences: 36976
Number of extensions: 40103
Number of successful extensions: 760
Number of sequences better than 10.0: 78
Number of HSP's better than 10.0 without gapping: 643
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 754
length of query: 83
length of database: 9,014,727
effective HSP length: 59
effective length of query: 24
effective length of database: 6,833,143
effective search space: 163995432
effective search space used: 163995432
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)
Medicago: description of AC121238.7