BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121233.6 - phase: 2
(123 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC79466 similar to PIR|T00839|T00839 hypothetical protein At2g17... 249 1e-67
TC86333 weakly similar to PIR|T00839|T00839 hypothetical protein... 166 2e-42
BE998732 weakly similar to PIR|T00839|T008 hypothetical protein ... 107 8e-38
TC86334 weakly similar to GP|10177132|dbj|BAB10422. senescence-a... 79 7e-25
TC86665 similar to GP|7594903|dbj|BAA88985.2 Ntdin {Nicotiana ta... 99 3e-22
BE203893 similar to GP|20197696|gb| senescence-associated protei... 96 3e-21
BG588641 similar to GP|20197696|gb| senescence-associated protei... 84 1e-17
TC78039 similar to PIR|T05875|T05875 hypothetical protein T29A15... 49 5e-07
TC86666 similar to GP|7594903|dbj|BAA88985.2 Ntdin {Nicotiana ta... 39 5e-04
TC87052 similar to GP|15010630|gb|AAK73974.1 At2g42220/T24P15.13... 37 0.001
TC84778 similar to GP|16323478|gb|AAL15233.1 putative peptidyl-p... 35 0.009
TC89250 weakly similar to GP|21592651|gb|AAM64600.1 rhodanese-li... 33 0.036
BI308335 similar to PIR|T05559|T05 hypothetical protein F22K18.5... 30 0.23
BQ137307 28 1.2
TC86070 weakly similar to GP|9294390|dbj|BAB02400.1 cytochrome P... 25 5.7
TC82883 25 7.5
TC88313 weakly similar to PIR|E84776|E84776 hypothetical protein... 25 7.5
TC88192 weakly similar to PIR|T05682|T05682 monooxygenase (EC 1.... 25 7.5
TC77566 similar to GP|310580|gb|AAA34017.1|| protein kinase 2 {G... 25 9.8
>TC79466 similar to PIR|T00839|T00839 hypothetical protein At2g17850
[imported] - Arabidopsis thaliana, partial (56%)
Length = 652
Score = 249 bits (637), Expect = 1e-67
Identities = 123/123 (100%), Positives = 123/123 (100%)
Frame = +3
Query: 1 SKTEVVTVDVLATKSLIKTTHVYLDVRTVEEFQKGHVDSEKIINIAYMFNTPEGRVKNPE 60
SKTEVVTVDVLATKSLIKTTHVYLDVRTVEEFQKGHVDSEKIINIAYMFNTPEGRVKNPE
Sbjct: 78 SKTEVVTVDVLATKSLIKTTHVYLDVRTVEEFQKGHVDSEKIINIAYMFNTPEGRVKNPE 257
Query: 61 FLKEVSSLCNKEDHLIVGCQSGVRSVYATADLLAEGFKDVYNMGGGYLEWVKKEFPVKIH 120
FLKEVSSLCNKEDHLIVGCQSGVRSVYATADLLAEGFKDVYNMGGGYLEWVKKEFPVKIH
Sbjct: 258 FLKEVSSLCNKEDHLIVGCQSGVRSVYATADLLAEGFKDVYNMGGGYLEWVKKEFPVKIH 437
Query: 121 VTE 123
VTE
Sbjct: 438 VTE 446
>TC86333 weakly similar to PIR|T00839|T00839 hypothetical protein At2g17850
[imported] - Arabidopsis thaliana, partial (58%)
Length = 941
Score = 166 bits (421), Expect = 2e-42
Identities = 78/117 (66%), Positives = 99/117 (83%)
Frame = +1
Query: 1 SKTEVVTVDVLATKSLIKTTHVYLDVRTVEEFQKGHVDSEKIINIAYMFNTPEGRVKNPE 60
S +VVT+DV A K+LI+T H+YLDVRTVEEF+KGHVD+ KIINI Y+ +TP+GRVKN
Sbjct: 274 SGAKVVTIDVHAAKNLIQTGHIYLDVRTVEEFEKGHVDATKIINIPYLLDTPKGRVKNLN 453
Query: 61 FLKEVSSLCNKEDHLIVGCQSGVRSVYATADLLAEGFKDVYNMGGGYLEWVKKEFPV 117
F+K+VSS C+KED L+VGCQSG RS AT++LLA+GFK+V+NMGGGY+EWV + PV
Sbjct: 454 FVKQVSSSCDKEDCLVVGCQSGKRSFSATSELLADGFKNVHNMGGGYMEWVSNKLPV 624
>BE998732 weakly similar to PIR|T00839|T008 hypothetical protein At2g17850
[imported] - Arabidopsis thaliana, partial (30%)
Length = 709
Score = 107 bits (267), Expect(2) = 8e-38
Identities = 51/77 (66%), Positives = 65/77 (84%)
Frame = +1
Query: 1 SKTEVVTVDVLATKSLIKTTHVYLDVRTVEEFQKGHVDSEKIINIAYMFNTPEGRVKNPE 60
S +VVT+DV A K+LI+T H+YLDVRTVEEF+KGHVD+ KIINI Y+ +TP+GRVKN
Sbjct: 151 SGAKVVTIDVHAAKNLIQTGHIYLDVRTVEEFEKGHVDATKIINIPYLLDTPKGRVKNLN 330
Query: 61 FLKEVSSLCNKEDHLIV 77
F+K+VSS C+KED L+V
Sbjct: 331 FVKQVSSSCDKEDCLVV 381
Score = 65.1 bits (157), Expect(2) = 8e-38
Identities = 27/43 (62%), Positives = 36/43 (82%)
Frame = +2
Query: 75 LIVGCQSGVRSVYATADLLAEGFKDVYNMGGGYLEWVKKEFPV 117
+ +GCQSG RS AT++LLA+GFK+V+NMGGGY+EWV + PV
Sbjct: 395 IFLGCQSGKRSFSATSELLADGFKNVHNMGGGYMEWVSNKLPV 523
>TC86334 weakly similar to GP|10177132|dbj|BAB10422. senescence-associated
protein sen1-like protein {Arabidopsis thaliana},
partial (53%)
Length = 593
Score = 79.0 bits (193), Expect(3) = 7e-25
Identities = 37/57 (64%), Positives = 47/57 (81%)
Frame = +1
Query: 1 SKTEVVTVDVLATKSLIKTTHVYLDVRTVEEFQKGHVDSEKIINIAYMFNTPEGRVK 57
S +VVT+DV A K+LI+T H+YLDVRTVEEF+KGHVD+ KIINI Y+ +TP+G K
Sbjct: 127 SGAKVVTIDVHAAKNLIQTGHIYLDVRTVEEFEKGHVDATKIINIPYLLDTPKGLPK 297
Score = 38.5 bits (88), Expect(3) = 7e-25
Identities = 17/27 (62%), Positives = 22/27 (80%)
Frame = +2
Query: 78 GCQSGVRSVYATADLLAEGFKDVYNMG 104
GCQSG RS AT++LLA+GFK+V+ G
Sbjct: 287 GCQSGKRSFSATSELLADGFKNVHQHG 367
Score = 31.6 bits (70), Expect(3) = 7e-25
Identities = 11/16 (68%), Positives = 13/16 (80%)
Frame = +3
Query: 102 NMGGGYLEWVKKEFPV 117
NMGGGY+EWV + PV
Sbjct: 360 NMGGGYMEWVSNKLPV 407
>TC86665 similar to GP|7594903|dbj|BAA88985.2 Ntdin {Nicotiana tabacum},
partial (60%)
Length = 821
Score = 99.4 bits (246), Expect = 3e-22
Identities = 52/112 (46%), Positives = 63/112 (55%)
Frame = +1
Query: 7 TVDVLATKSLIKTTHVYLDVRTVEEFQKGHVDSEKIINIAYMFNTPEGRVKNPEFLKEVS 66
+V V L+ H YLDVRT EEF GH INI YM+ G KN F+KEVS
Sbjct: 355 SVPVRVAHELLLAGHKYLDVRTTEEFNAGHAPGA--INIPYMYKVGSGMTKNSNFVKEVS 528
Query: 67 SLCNKEDHLIVGCQSGVRSVYATADLLAEGFKDVYNMGGGYLEWVKKEFPVK 118
S KED +IVGCQ G RS+ A DLLA GF + ++ GGY W + P +
Sbjct: 529 SHFRKEDEVIVGCQLGKRSMMAATDLLAAGFTGLTDIAGGYAAWTQNGLPTE 684
>BE203893 similar to GP|20197696|gb| senescence-associated protein
{Arabidopsis thaliana}, partial (12%)
Length = 643
Score = 95.9 bits (237), Expect = 3e-21
Identities = 50/90 (55%), Positives = 65/90 (71%), Gaps = 1/90 (1%)
Frame = +1
Query: 4 EVVTVDVLATKSLIKTT-HVYLDVRTVEEFQKGHVDSEKIINIAYMFNTPEGRVKNPEFL 62
+VVT+DV ATK L+ ++ + YLDVRTVEEF K HV E IN+ Y+F+T EGRVKNP+F+
Sbjct: 169 DVVTLDVHATKDLLDSSGYNYLDVRTVEEFNKSHV--ENAINVPYLFSTEEGRVKNPDFV 342
Query: 63 KEVSSLCNKEDHLIVGCQSGVRSVYATADL 92
+V ++ EDHLIV C +G RS A DL
Sbjct: 343 NQVEAIYKSEDHLIVACNAGGRSSRAWVDL 432
>BG588641 similar to GP|20197696|gb| senescence-associated protein
{Arabidopsis thaliana}, partial (41%)
Length = 516
Score = 84.0 bits (206), Expect = 1e-17
Identities = 41/70 (58%), Positives = 50/70 (70%)
Frame = +2
Query: 23 YLDVRTVEEFQKGHVDSEKIINIAYMFNTPEGRVKNPEFLKEVSSLCNKEDHLIVGCQSG 82
YLDVRTVEEF K HV E IN+ Y+F+T EGRVKNP+F+ +V ++ EDHLIV C +G
Sbjct: 77 YLDVRTVEEFNKSHV--ENAINVPYLFSTEEGRVKNPDFVNQVEAIYKSEDHLIVACNAG 250
Query: 83 VRSVYATADL 92
RS A DL
Sbjct: 251 GRSSRAWVDL 280
>TC78039 similar to PIR|T05875|T05875 hypothetical protein T29A15.190 -
Arabidopsis thaliana, partial (69%)
Length = 1080
Score = 48.9 bits (115), Expect = 5e-07
Identities = 33/126 (26%), Positives = 53/126 (41%), Gaps = 31/126 (24%)
Frame = +1
Query: 22 VYLDVRTVEEFQKGHVDSEKIINIAYM-----------------FNTPEGRVKNPEFLKE 64
V LDVR EF++GH + + + F G +NPEF+K
Sbjct: 484 VILDVRPEAEFKEGHPPDAINVQVYRLIKEWTAWDIARRAAFAFFGIFSGTEENPEFIKS 663
Query: 65 VSSLCNKEDHLIVGCQSG--------------VRSVYATADLLAEGFKDVYNMGGGYLEW 110
V +K +IV C +G RS+ A L+ G+ +V+++ GG +W
Sbjct: 664 VGEQLDKNAKIIVACSAGGTMKPTQNLPQGQQSRSLIAAYLLVLNGYNNVFHLKGGLYKW 843
Query: 111 VKKEFP 116
K++ P
Sbjct: 844 FKEDLP 861
>TC86666 similar to GP|7594903|dbj|BAA88985.2 Ntdin {Nicotiana tabacum},
partial (26%)
Length = 787
Score = 38.9 bits (89), Expect = 5e-04
Identities = 20/40 (50%), Positives = 22/40 (55%)
Frame = +2
Query: 15 SLIKTTHVYLDVRTVEEFQKGHVDSEKIINIAYMFNTPEG 54
SL+ H YLDVRT EEF GH INI YM+ G
Sbjct: 524 SLLLAGHKYLDVRTTEEFNAGHAPG--XINIPYMYKVGSG 637
>TC87052 similar to GP|15010630|gb|AAK73974.1 At2g42220/T24P15.13
{Arabidopsis thaliana}, partial (77%)
Length = 993
Score = 37.4 bits (85), Expect = 0.001
Identities = 35/134 (26%), Positives = 56/134 (41%), Gaps = 23/134 (17%)
Frame = +1
Query: 2 KTEVVTVDVLATKSLIKTT-HVYLDVRTVEEFQKGHVDSEKIINI--------------- 45
K E+ V K L+K + LDVR ++++ H+ + + +
Sbjct: 148 KAEIKFVTADDAKELVKVDGYNVLDVRDKSQYERAHIKTCYHVPLFVENTDNDPGTFLLR 327
Query: 46 -------AYMFNTPEGRVKNPEFLKEVSSLCNKEDHLIVGCQSGVRSVYATADLLAEGFK 98
F P R NP+F++ V S E L++ CQ G+RS A L GF+
Sbjct: 328 TVHNNFSGLFFGIPFTR-PNPDFVQSVKSQIQPETKLLIVCQEGLRSAAAANKLEDAGFQ 504
Query: 99 DVYNMGGGYLEWVK 112
+V + G L+ VK
Sbjct: 505 NVACITSG-LQTVK 543
>TC84778 similar to GP|16323478|gb|AAL15233.1 putative peptidyl-prolyl
cis-trans isomerase {Arabidopsis thaliana}, partial
(58%)
Length = 821
Score = 34.7 bits (78), Expect = 0.009
Identities = 18/53 (33%), Positives = 28/53 (51%)
Frame = +3
Query: 64 EVSSLCNKEDHLIVGCQSGVRSVYATADLLAEGFKDVYNMGGGYLEWVKKEFP 116
E++S + + V C GVRS+ L ++GF+ VYN+ GG + K P
Sbjct: 375 EINSKFDLQKDTYVLCHHGVRSLQVARWLQSQGFRKVYNISGGIHAYAVKVDP 533
>TC89250 weakly similar to GP|21592651|gb|AAM64600.1 rhodanese-like family
protein {Arabidopsis thaliana}, partial (78%)
Length = 1039
Score = 32.7 bits (73), Expect = 0.036
Identities = 17/51 (33%), Positives = 31/51 (60%), Gaps = 1/51 (1%)
Frame = +3
Query: 58 NPEFLKEVSSLC-NKEDHLIVGCQSGVRSVYATADLLAEGFKDVYNMGGGY 107
N EFL +V ++ K ++V C G+RS+ AT+ L G++++ + GG+
Sbjct: 405 NSEFLSQVENVVPGKGTKVLVACGEGLRSMTATSKLYNGGYRNLGWLVGGF 557
>BI308335 similar to PIR|T05559|T05 hypothetical protein F22K18.50 -
Arabidopsis thaliana, partial (38%)
Length = 699
Score = 30.0 bits (66), Expect = 0.23
Identities = 15/41 (36%), Positives = 24/41 (57%)
Frame = +2
Query: 61 FLKEVSSLCNKEDHLIVGCQSGVRSVYATADLLAEGFKDVY 101
FL +V+ K+ LIV CQ G+RS+ A L G+ +++
Sbjct: 530 FLPKVAEKFPKDAELIVACQKGLRSLAACELLYNAGYTNLF 652
>BQ137307
Length = 912
Score = 27.7 bits (60), Expect = 1.2
Identities = 14/42 (33%), Positives = 20/42 (47%)
Frame = -3
Query: 62 LKEVSSLCNKEDHLIVGCQSGVRSVYATADLLAEGFKDVYNM 103
L + S+C + D + VGC GVR V A +YN+
Sbjct: 430 LCQFESVCERLDRIRVGCGQGVRGVRGYRVERASRMNSLYNI 305
>TC86070 weakly similar to GP|9294390|dbj|BAB02400.1 cytochrome P450
{Arabidopsis thaliana}, partial (44%)
Length = 1831
Score = 25.4 bits (54), Expect = 5.7
Identities = 14/37 (37%), Positives = 20/37 (53%)
Frame = -2
Query: 42 IINIAYMFNTPEGRVKNPEFLKEVSSLCNKEDHLIVG 78
II+ M +TP G V++P F+ SL D +I G
Sbjct: 1347 IIDTGTMSSTPAGSVRSPSFV----SLRRNRDSIITG 1249
>TC82883
Length = 571
Score = 25.0 bits (53), Expect = 7.5
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = -3
Query: 67 SLCNKEDHLIVGCQSGVRSVYATADLLAEG 96
S+C+ HLI Q + S+Y +LL +G
Sbjct: 476 SICDPIQHLISKTQQNLTSIYHINNLLQDG 387
>TC88313 weakly similar to PIR|E84776|E84776 hypothetical protein At2g36070
[imported] - Arabidopsis thaliana, partial (31%)
Length = 1201
Score = 25.0 bits (53), Expect = 7.5
Identities = 17/47 (36%), Positives = 24/47 (50%), Gaps = 1/47 (2%)
Frame = +2
Query: 56 VKNPEFLKEVSSLCNKEDHLIVGCQSGVR-SVYATADLLAEGFKDVY 101
VKNPEF K V L K + L G + G++ T + L + F V+
Sbjct: 272 VKNPEFQKSVKELKEKAEEL-KGVKEGLKEKTKQTTEQLYKQFDGVW 409
>TC88192 weakly similar to PIR|T05682|T05682 monooxygenase (EC 1.-.-.-) 2 -
Arabidopsis thaliana, partial (61%)
Length = 1557
Score = 25.0 bits (53), Expect = 7.5
Identities = 12/25 (48%), Positives = 15/25 (60%), Gaps = 5/25 (20%)
Frame = -2
Query: 98 KDVYNMGGGYLEW-----VKKEFPV 117
KDVY GGGYL+ + K FP+
Sbjct: 323 KDVYVCGGGYLQHQQHPRLSKHFPI 249
>TC77566 similar to GP|310580|gb|AAA34017.1|| protein kinase 2 {Glycine
max}, partial (88%)
Length = 1502
Score = 24.6 bits (52), Expect = 9.8
Identities = 15/44 (34%), Positives = 27/44 (61%)
Frame = -3
Query: 52 PEGRVKNPEFLKEVSSLCNKEDHLIVGCQSGVRSVYATADLLAE 95
P GR+ N + +++S+LC + IVG +GV + Y + + L+E
Sbjct: 810 PLGRIGNKDARQKISALC--RNAYIVG--NGVLNTYYSPNSLSE 691
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.316 0.135 0.386
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,518,852
Number of Sequences: 36976
Number of extensions: 41042
Number of successful extensions: 203
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 198
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 198
length of query: 123
length of database: 9,014,727
effective HSP length: 84
effective length of query: 39
effective length of database: 5,908,743
effective search space: 230440977
effective search space used: 230440977
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)
Medicago: description of AC121233.6