BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149600.13 + phase: 0 /pseudo
(232 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC10200 similar to UP|AAR01750 (AAR01750) Expressed protein, par... 148 9e-37
CN825386 133 3e-32
BP040908 127 2e-30
BP035322 115 5e-27
AV408775 69 5e-13
TC18247 similar to UP|BTG1_HUMAN (P31607) BTG1 protein (B-cell t... 69 9e-13
TC11170 weakly similar to UP|Q9FKT4 (Q9FKT4) RNA-binding protein... 36 0.005
TC14734 UP|Q9FKT4 (Q9FKT4) RNA-binding protein-like, partial (9%) 30 0.27
TC8322 weakly similar to UP|ATP7_ARATH (Q9SJ12) Probable ATP syn... 27 3.0
CN825587 27 3.9
TC8653 similar to UP|AAS46232 (AAS46232) Methionine sulfoxide re... 25 8.8
>TC10200 similar to UP|AAR01750 (AAR01750) Expressed protein, partial (30%)
Length = 615
Score = 148 bits (373), Expect = 9e-37
Identities = 75/88 (85%), Positives = 82/88 (92%)
Frame = +3
Query: 145 GFEHLNEPLHILIEAELPVNVVDLRLRQAQEIIEELLKPVDESQDIYKRQQLRELAMLNS 204
G+EH +EPLHILIEAELP +VD+RLRQAQEIIEE+LKPVDESQD YKRQQLRE AMLNS
Sbjct: 3 GYEHXSEPLHILIEAELPAXIVDVRLRQAQEIIEEILKPVDESQDFYKRQQLRERAMLNS 182
Query: 205 SFREESPQLSGSLSPFTSNEMIKRAKTD 232
+FREESPQLSGS+SPFTSNE IKRAKTD
Sbjct: 183 NFREESPQLSGSVSPFTSNE-IKRAKTD 263
>CN825386
Length = 670
Score = 133 bits (334), Expect = 3e-32
Identities = 75/167 (44%), Positives = 103/167 (60%), Gaps = 14/167 (8%)
Frame = +3
Query: 1 MQVLPLCTRLLNQEILRASGKSGLMQNQGFSDYDRVQFGSTKPSLMPSLDTTS-SFTGWN 59
+QVLP CTRL+ QEI R +G NQGF ++DR + S SL ++ GW
Sbjct: 183 VQVLPQCTRLITQEIRRITGF-----NQGFIEHDRPESDSPFRSLSQHPNSRPMDLEGWP 347
Query: 60 SL------------SHEGLNVDWQRAPAISNSHIVKKMLRLDIPHDNHPT-FNFVGRLLG 106
++ S + + W + + +VK+++RLD+P + +P +NFVGR+LG
Sbjct: 348 AVPIEDNGNLQRIASFQAPPMGWPGTQGVPTTPVVKRVIRLDVPVEKYPNQYNFVGRILG 527
Query: 107 PRGNSLKRVEATTGCRVFIRGKGSIKDFDKEELLRGRPGFEHLNEPL 153
PRGNSLKRVEA T CRV+IRG GS+KD KEE L+ +PG+EHLNEPL
Sbjct: 528 PRGNSLKRVEAMTECRVYIRGCGSVKDSIKEEKLKDKPGYEHLNEPL 668
>BP040908
Length = 523
Score = 127 bits (319), Expect = 2e-30
Identities = 72/133 (54%), Positives = 87/133 (65%), Gaps = 12/133 (9%)
Frame = -3
Query: 1 MQVLPLCTRLLNQEILRASGKSGLMQNQGFSDYDRVQFGSTKP----SLMPSLDTTSSFT 56
MQVLP C RLLNQEILR SG ++ NQGF D+DR++ S P +LM ++ T
Sbjct: 404 MQVLPTCNRLLNQEILRVSG---MLSNQGFGDFDRLRHRSPSPMAPSNLMSNVPGTG-LG 237
Query: 57 GWNSLSHE------GLNVDWQRAPAISNSHIVKKMLRLDIPHDNHPTFNFVGRLLGPRGN 110
GWN L E G+ +DWQ APA S+ V+++LRL+IP D P FNFVGRLLGPRGN
Sbjct: 236 GWNGLQQERLCGTPGMTMDWQVAPASPTSYTVRRILRLEIPVDTFPNFNFVGRLLGPRGN 57
Query: 111 SLKRVEA--TTGC 121
SLKRVEA GC
Sbjct: 56 SLKRVEAQQVAGC 18
Score = 30.4 bits (67), Expect = 0.27
Identities = 15/34 (44%), Positives = 18/34 (52%)
Frame = -2
Query: 96 PTFNFVGRLLGPRGNSLKRVEATTGCRVFIRGKG 129
P F F + G + +TTGCRVFIRG G
Sbjct: 102 PQFQFCWKTSGT*RQFSETGRSTTGCRVFIRGTG 1
>BP035322
Length = 556
Score = 115 bits (289), Expect = 5e-27
Identities = 59/91 (64%), Positives = 71/91 (77%), Gaps = 7/91 (7%)
Frame = +1
Query: 1 MQVLPLCTRLLNQEILRASGKSGLMQNQGFSDYDRVQFGSTKPSLMPSLDTTSSFTGWNS 60
MQVLPLC+RLLNQEILRASGK+G++QNQGFSD+DR+Q S P M S + T +F+GWNS
Sbjct: 289 MQVLPLCSRLLNQEILRASGKNGMLQNQGFSDFDRLQVLS--PGHMTSSELTPNFSGWNS 462
Query: 61 LSHE-------GLNVDWQRAPAISNSHIVKK 84
LSHE GLN+DWQ PA+ +SHIVKK
Sbjct: 463 LSHEQRLAGAQGLNMDWQAPPAVPSSHIVKK 555
>AV408775
Length = 427
Score = 69.3 bits (168), Expect = 5e-13
Identities = 32/35 (91%), Positives = 34/35 (96%)
Frame = +1
Query: 1 MQVLPLCTRLLNQEILRASGKSGLMQNQGFSDYDR 35
MQVLPLCTRLLNQEILR SGK+GL+QNQGFSDYDR
Sbjct: 322 MQVLPLCTRLLNQEILRVSGKNGLIQNQGFSDYDR 426
>TC18247 similar to UP|BTG1_HUMAN (P31607) BTG1 protein (B-cell
translocation gene 1 protein), partial (8%)
Length = 543
Score = 68.6 bits (166), Expect = 9e-13
Identities = 39/88 (44%), Positives = 52/88 (58%), Gaps = 10/88 (11%)
Frame = +2
Query: 2 QVLPLCTRLLNQEILRASGKSGLMQNQGFSDYDRVQFGSTKPSLMPSLDTTSSFT---GW 58
QVLP+C RL+NQEILR S ++ NQGF D+DR++ S P ++ + S T GW
Sbjct: 284 QVLPICNRLINQEILRV---SRMLSNQGFGDFDRLRHRSPSPMASSNIMSNVSGTGLGGW 454
Query: 59 NSLSHE-------GLNVDWQRAPAISNS 79
NSL E G+ +DWQ APA +S
Sbjct: 455 NSLQQEQRFSGPPGMTMDWQGAPASPSS 538
>TC11170 weakly similar to UP|Q9FKT4 (Q9FKT4) RNA-binding protein-like,
partial (32%)
Length = 613
Score = 36.2 bits (82), Expect = 0.005
Identities = 29/98 (29%), Positives = 41/98 (41%), Gaps = 8/98 (8%)
Frame = +2
Query: 1 MQVLPLCTRLLNQEILRASGKSGLMQNQGFSDYDRVQFGSTKPSLMPSLDTTSSFTGW-- 58
M VLP C RL+NQEILR + L+ N R++ S + + + GW
Sbjct: 329 MAVLPHCYRLINQEILRV---TTLLGNASVLGQSRLEHASPLATGGIFSNGGADVNGWAS 499
Query: 59 ------NSLSHEGLNVDWQRAPAISNSHIVKKMLRLDI 90
SL W S+ +VKK +R+DI
Sbjct: 500 RFQSEMPSLLQSSATPSWLSPQGSSSGLVVKKTMRVDI 613
>TC14734 UP|Q9FKT4 (Q9FKT4) RNA-binding protein-like, partial (9%)
Length = 598
Score = 30.4 bits (67), Expect = 0.27
Identities = 14/27 (51%), Positives = 20/27 (73%)
Frame = +2
Query: 205 SFREESPQLSGSLSPFTSNEMIKRAKT 231
+ REE +SGS+SPF ++ +KRAKT
Sbjct: 5 TLREEGSPMSGSVSPFHNSLGMKRAKT 85
>TC8322 weakly similar to UP|ATP7_ARATH (Q9SJ12) Probable ATP synthase 24
kDa subunit, mitochondrial precursor , partial (94%)
Length = 1089
Score = 26.9 bits (58), Expect = 3.0
Identities = 17/50 (34%), Positives = 29/50 (58%)
Frame = +1
Query: 146 FEHLNEPLHILIEAELPVNVVDLRLRQAQEIIEELLKPVDESQDIYKRQQ 195
F+ +N+ L I E +LP L L+ A+ +EEL K V E+ + K+++
Sbjct: 589 FDKINKQLGIRKE-DLPKYEEQLELKLAKAQLEELKKDVVEAMETQKKRE 735
>CN825587
Length = 663
Score = 26.6 bits (57), Expect = 3.9
Identities = 25/100 (25%), Positives = 47/100 (47%), Gaps = 2/100 (2%)
Frame = +2
Query: 122 RVFIRGKGSIKDFDKEELLRGRPGFEHLNEPLHILIEAELPVNVVDLRLRQAQEIIEELL 181
+V RGK KD D +++ R PGF + +++ EA + DL+ EI + L
Sbjct: 278 QVHSRGKALAKDVDFDKIARRTPGFTGA-DLQNLMNEAAILAARRDLKEISKDEISDALE 454
Query: 182 KPV--DESQDIYKRQQLRELAMLNSSFREESPQLSGSLSP 219
+ + E ++ ++ ++L ++ E L G+L P
Sbjct: 455 RIIAGPEKKNAVVSEEKKKLV----AYHEAGHALVGALMP 562
>TC8653 similar to UP|AAS46232 (AAS46232) Methionine sulfoxide reductase A,
partial (71%)
Length = 1175
Score = 25.4 bits (54), Expect = 8.8
Identities = 11/31 (35%), Positives = 15/31 (47%)
Frame = -2
Query: 95 HPTFNFVGRLLGPRGNSLKRVEATTGCRVFI 125
HP + G+L P S K + TGCR +
Sbjct: 439 HPRYPLEGQLNTPEATSTKLGKLLTGCRYVV 347
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.135 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,475,023
Number of Sequences: 28460
Number of extensions: 43346
Number of successful extensions: 209
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 201
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 202
length of query: 232
length of database: 4,897,600
effective HSP length: 87
effective length of query: 145
effective length of database: 2,421,580
effective search space: 351129100
effective search space used: 351129100
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)
Medicago: description of AC149600.13