BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149582.6 + phase: 0
(377 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP028499 50 5e-07
AV422434 45 2e-05
BP069765 41 3e-04
BP056337 32 0.13
TC9116 similar to UP|Q9ZW85 (Q9ZW85) 3-isopropylmalate dehydrata... 32 0.13
AV779394 31 0.30
TC17650 30 0.66
TC16741 weakly similar to UP|Q9FNS9 (Q9FNS9) Fructan 1-exohydrol... 30 0.86
TC16133 similar to UP|Q39449 (Q39449) Specific tissue protein 1,... 28 1.9
TC8594 similar to UP|GCSH_PEA (P16048) Glycine cleavage system H... 28 1.9
AV779129 28 2.5
TC11028 28 2.5
BP051299 28 3.3
AV410651 28 3.3
BP076687 28 3.3
TC14859 27 4.3
BP039012 27 4.3
CB827691 27 4.3
BP066916 27 4.3
BP048756 27 5.6
>BP028499
Length = 491
Score = 50.4 bits (119), Expect = 5e-07
Identities = 37/103 (35%), Positives = 50/103 (47%), Gaps = 5/103 (4%)
Frame = +2
Query: 6 DWSHLPKELWSKIGKYLDNHIDILRFRSVCESFR----SSIPPSHPNSPSFPLQIPHTIF 61
DWS LP EL I + L++ + +LRFRSVC ++R SS P H +FP H
Sbjct: 116 DWSQLPAELLHLISQRLNSPLYLLRFRSVCSTWRHSSASSFIPIHHFPFNFPPLSDHDHH 295
Query: 62 D-SLYYLHQSTIYLIEPTNGSSNFNLPPLAPSYTKGWLIKVEE 103
D S + L + T+ LI P PP WL+K+ E
Sbjct: 296 DASSFSLTKRTVVLITPP--------PPPHHQTQTPWLVKIGE 400
>AV422434
Length = 422
Score = 45.4 bits (106), Expect = 2e-05
Identities = 34/102 (33%), Positives = 49/102 (47%), Gaps = 15/102 (14%)
Frame = +3
Query: 5 VDWSHLPKELWSKIGKYLDNHIDILRFRSVCESFR----SSIPPSH--PNS--------- 49
VDWS LP EL I + LD+ + +LRFRSVC ++R S++ P H PN
Sbjct: 54 VDWSQLPTELLHLISQRLDSPLYLLRFRSVCSTWRRSSSSAVTPLHHFPNPLRRHRFHLL 233
Query: 50 PSFPLQIPHTIFDSLYYLHQSTIYLIEPTNGSSNFNLPPLAP 91
P ++PH + +++ L+ P N PPL P
Sbjct: 234 PLPTHRLPH--HPTATPTNRTPNPLVVPNQPRQRLNPPPLRP 353
>BP069765
Length = 417
Score = 41.2 bits (95), Expect = 3e-04
Identities = 18/39 (46%), Positives = 25/39 (63%)
Frame = -2
Query: 303 WVDVKNLRDRAFVLSRSCNFSVSAKELIGYQGNCIYFKD 341
WV V+N+RDR + C FSVSA +L +GN + F+D
Sbjct: 410 WVHVRNVRDRVLFIGDDCAFSVSASDLGVDEGNYVVFRD 294
>BP056337
Length = 550
Score = 32.3 bits (72), Expect = 0.13
Identities = 21/61 (34%), Positives = 30/61 (48%), Gaps = 4/61 (6%)
Frame = +1
Query: 38 FRSSIPPSHPN----SPSFPLQIPHTIFDSLYYLHQSTIYLIEPTNGSSNFNLPPLAPSY 93
F+++ PPS PN SPSFP P T S Y S+ P G++ ++ P P +
Sbjct: 376 FKATPPPSIPNPLNSSPSFPAPSPSTPPPSSYPTPSSS---YPPPTGTNPYHFPHYLPPF 546
Query: 94 T 94
T
Sbjct: 547 T 549
>TC9116 similar to UP|Q9ZW85 (Q9ZW85) 3-isopropylmalate dehydratase, small
subunit, partial (25%)
Length = 426
Score = 32.3 bits (72), Expect = 0.13
Identities = 28/93 (30%), Positives = 37/93 (39%)
Frame = +1
Query: 39 RSSIPPSHPNSPSFPLQIPHTIFDSLYYLHQSTIYLIEPTNGSSNFNLPPLAPSYTKGWL 98
RS P SPS PL H++F L +LH ++ + N L PL S+ L
Sbjct: 22 RSPPQPFLKTSPSLPLTNSHSLFHLLLHLHLTSSH-SPLLNQP*PLALSPLTKSHAPTLL 198
Query: 99 IKVEESNNNPLKSLLSPISDCKLSYPLDSNNSS 131
+ N P S S S S P S+ S
Sbjct: 199 LPPPPPNPQPHPSTASATSSATTSTPTRSSPPS 297
>AV779394
Length = 470
Score = 31.2 bits (69), Expect = 0.30
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = -3
Query: 88 PLAPSYTKGWLIKVEESNNNPLKSLLSP 115
P P+ T GW+IKV+ S + L SLL P
Sbjct: 231 PHKPTTTDGWMIKVKPSRPSALDSLLGP 148
>TC17650
Length = 826
Score = 30.0 bits (66), Expect = 0.66
Identities = 16/47 (34%), Positives = 27/47 (57%), Gaps = 4/47 (8%)
Frame = +2
Query: 288 VECFKVHMLDEEWGKW---VDVKNLRDRAF-VLSRSCNFSVSAKELI 330
+ CF + +L E W KW VD+++ R+ +F +LS FS + L+
Sbjct: 173 IPCFSISLLKEGWKKWENFVDMQSCRNGSFYLLSYFTIFSAVLRSLV 313
>TC16741 weakly similar to UP|Q9FNS9 (Q9FNS9) Fructan 1-exohydrolase I
precursor, partial (25%)
Length = 680
Score = 29.6 bits (65), Expect = 0.86
Identities = 18/51 (35%), Positives = 24/51 (46%)
Frame = -3
Query: 20 KYLDNHIDILRFRSVCESFRSSIPPSHPNSPSFPLQIPHTIFDSLYYLHQS 70
KYL + I + +VC + S PN PS PL FD+L LH +
Sbjct: 165 KYLLEDLYIRKKTAVCSLWSSEANADKPNGPSLPL------FDALSSLHSN 31
>TC16133 similar to UP|Q39449 (Q39449) Specific tissue protein 1, partial
(40%)
Length = 934
Score = 28.5 bits (62), Expect = 1.9
Identities = 15/47 (31%), Positives = 24/47 (50%)
Frame = -3
Query: 204 ENNFFYDDVIVFKGLFYVTDRWGTISWIDISSLRLIQFAPPLCGFGN 250
E+ F YD IV + ++T +G +SW D+ +Q L FG+
Sbjct: 281 EHLFLYDIFIVCNHMLFITFVFGILSWFDLR----LQLKKALNSFGH 153
>TC8594 similar to UP|GCSH_PEA (P16048) Glycine cleavage system H protein,
mitochondrial precursor , complete
Length = 742
Score = 28.5 bits (62), Expect = 1.9
Identities = 12/23 (52%), Positives = 16/23 (69%)
Frame = +3
Query: 93 YTKGWLIKVEESNNNPLKSLLSP 115
Y GW+IKV+ S+ + L SLL P
Sbjct: 426 YEDGWMIKVKPSSPSELDSLLGP 494
>AV779129
Length = 556
Score = 28.1 bits (61), Expect = 2.5
Identities = 18/55 (32%), Positives = 25/55 (44%), Gaps = 13/55 (23%)
Frame = +1
Query: 91 PSYTKGWLIKVEESNNNPLKS-------------LLSPISDCKLSYPLDSNNSSP 132
P + WL + +NNPLKS LLS +SDC S+ +S+P
Sbjct: 337 PFHPLFWLESYLQFSNNPLKSDSVTSMVLPSSPKLLSELSDCSFSFNNSLYSSNP 501
>TC11028
Length = 918
Score = 28.1 bits (61), Expect = 2.5
Identities = 18/46 (39%), Positives = 25/46 (54%)
Frame = +1
Query: 75 IEPTNGSSNFNLPPLAPSYTKGWLIKVEESNNNPLKSLLSPISDCK 120
I+P NGS +F L PS G+L VE+++ P SL + CK
Sbjct: 4 IQPNNGSDSF----LLPSGNNGFL--VEDTDGRPSSSLDTRRMSCK 123
>BP051299
Length = 539
Score = 27.7 bits (60), Expect = 3.3
Identities = 14/34 (41%), Positives = 23/34 (67%)
Frame = +2
Query: 9 HLPKELWSKIGKYLDNHIDILRFRSVCESFRSSI 42
HLP+E+W++I L +++ SVC+S+RS I
Sbjct: 2 HLPQEIWAEILHRLPPK-TLVKCTSVCKSWRSLI 100
>AV410651
Length = 294
Score = 27.7 bits (60), Expect = 3.3
Identities = 14/28 (50%), Positives = 16/28 (57%)
Frame = -2
Query: 43 PPSHPNSPSFPLQIPHTIFDSLYYLHQS 70
PP H +S FPL PH +SL LH S
Sbjct: 230 PPQHHHSSFFPLTFPHP--NSLLELHYS 153
>BP076687
Length = 389
Score = 27.7 bits (60), Expect = 3.3
Identities = 17/35 (48%), Positives = 23/35 (65%)
Frame = -3
Query: 8 SHLPKELWSKIGKYLDNHIDILRFRSVCESFRSSI 42
SHLP+EL +I L +LRF+SVC+S+ S I
Sbjct: 387 SHLPEELIPQILLRLPVR-SLLRFKSVCKSWLSLI 286
>TC14859
Length = 601
Score = 27.3 bits (59), Expect = 4.3
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = -3
Query: 340 KDTFNARMYNLDDNIIITIDLDPCIDKTLLSHS 372
K F + +YN N+ +T++L P + TLL+ S
Sbjct: 494 KSLFFSSLYNFAPNVNLTVNLKPNLTLTLLTFS 396
>BP039012
Length = 582
Score = 27.3 bits (59), Expect = 4.3
Identities = 28/98 (28%), Positives = 43/98 (43%), Gaps = 4/98 (4%)
Frame = +1
Query: 40 SSIPPSHPNSPSFPLQIPHTIFDSLYYLHQS--TIYLIEPTNGSSNFNL--PPLAPSYTK 95
SS PP P +P + L PH + S ++ + + L+ P S +L PP P K
Sbjct: 91 SSAPPPPPLTPHYSLSQPHLLH*SNLQINVNPPPLPLLPPPASHSGGSLSPPPPTPPPHK 270
Query: 96 GWLIKVEESNNNPLKSLLSPISDCKLSYPLDSNNSSPI 133
++ + L LSP K S S++S+PI
Sbjct: 271 MTVLHPLLHLHLHLPPSLSPSPTSKNSSTSRSSSSAPI 384
>CB827691
Length = 419
Score = 27.3 bits (59), Expect = 4.3
Identities = 10/18 (55%), Positives = 13/18 (71%)
Frame = +2
Query: 43 PPSHPNSPSFPLQIPHTI 60
PP+ PNS SFP + H+I
Sbjct: 188 PPTLPNSSSFPTHVSHSI 241
>BP066916
Length = 529
Score = 27.3 bits (59), Expect = 4.3
Identities = 21/94 (22%), Positives = 40/94 (42%)
Frame = +3
Query: 128 NNSSPIIWNLLNYRVIELCKSYTFQKTLYSPSYVSKVVFYPNSPRINVGDCVACCIFQEG 187
++ S IWN+ Y L+S Y++ + F I+ C C+ G
Sbjct: 33 SSPSXTIWNIRLY--------------LFSEMYINTL*F*G----IDTNFCKVACLSLNG 158
Query: 188 KLGFMKYGDTKWKLVDENNFFYDDVIVFKGLFYV 221
+ Y +K V ++F+Y D+ + G+F++
Sbjct: 159 R-----YDTASYKQVQVDSFWYTDMSLSXGIFFL 245
>BP048756
Length = 547
Score = 26.9 bits (58), Expect = 5.6
Identities = 8/30 (26%), Positives = 14/30 (46%)
Frame = +3
Query: 303 WVDVKNLRDRAFVLSRSCNFSVSAKELIGY 332
W D +++ F C + +K L+GY
Sbjct: 171 WFDTSEMKEEYFTEDEGCELHIVSKSLVGY 260
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.140 0.452
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,814,265
Number of Sequences: 28460
Number of extensions: 148010
Number of successful extensions: 911
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 906
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 910
length of query: 377
length of database: 4,897,600
effective HSP length: 92
effective length of query: 285
effective length of database: 2,279,280
effective search space: 649594800
effective search space used: 649594800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 56 (26.2 bits)
Medicago: description of AC149582.6