BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149574.9 + phase: 2 /pseudo
(88 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8603 similar to UP|Q8LBJ5 (Q8LBJ5) Nucleic acid binding protei... 84 3e-18
TC8119 similar to UP|Q9FFF5 (Q9FFF5) Nucleic acid binding protei... 66 1e-12
BP049886 61 3e-11
TC8118 similar to GB|AAN28771.1|23505823|AY143832 At3g11200/F11B... 60 5e-11
TC15130 similar to UP|O81488 (O81488) F9D12.13 protein, partial ... 59 2e-10
TC18764 similar to UP|Q8LBJ5 (Q8LBJ5) Nucleic acid binding prote... 48 3e-07
TC10767 similar to UP|Q8LBJ5 (Q8LBJ5) Nucleic acid binding prote... 43 9e-06
TC10171 similar to UP|Q9M2B4 (Q9M2B4) Nucleic acid binding prote... 31 0.044
TC14970 similar to UP|O24294 (O24294) Legumin (Minor small) prec... 30 0.098
TC17533 similar to UP|Q9ZS85 (Q9ZS85) T4B21.1 protein, partial (... 29 0.13
AV418055 29 0.13
TC12890 homologue to UP|Q40359 (Q40359) Alfin-1, partial (23%) 28 0.22
AV418706 27 0.48
TC16074 similar to UP|Q8H9E8 (Q8H9E8) Resistant specific protein... 27 0.48
TC8321 similar to UP|AAS46230 (AAS46230) Peroxiredoxin Q, partia... 27 0.48
TC10025 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein... 27 0.48
TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly pr... 27 0.63
CN825457 27 0.83
AV775735 27 0.83
TC9642 27 0.83
>TC8603 similar to UP|Q8LBJ5 (Q8LBJ5) Nucleic acid binding protein-like,
partial (88%)
Length = 1232
Score = 84.3 bits (207), Expect = 3e-18
Identities = 47/87 (54%), Positives = 57/87 (65%), Gaps = 5/87 (5%)
Frame = +1
Query: 1 KRLFTPINDLPTIFEVVTGSAKKQTKEKPSVSSHNSNKSKSGSKARGSEL-AKYSKPPAK 59
KRLF INDLPTIFEVVT KK KEK SVS+++ +KSKS SKAR +E + SK
Sbjct: 490 KRLFNMINDLPTIFEVVTNPTKKPVKEKSSVSNNSGSKSKSSSKARAAETQGRQSKVSLS 669
Query: 60 EDD----EEVDDEEEYQGECTACGENY 82
+D+ EE DD+E C ACG+NY
Sbjct: 670 KDEDEGIEEQDDDEHGDALCGACGDNY 750
>TC8119 similar to UP|Q9FFF5 (Q9FFF5) Nucleic acid binding protein-like,
partial (85%)
Length = 1249
Score = 65.9 bits (159), Expect = 1e-12
Identities = 36/82 (43%), Positives = 50/82 (60%)
Frame = +1
Query: 1 KRLFTPINDLPTIFEVVTGSAKKQTKEKPSVSSHNSNKSKSGSKARGSELAKYSKPPAKE 60
KRLF+ INDLPT+FEVVT +K K+KP+V S +KS+ G+K K S P +
Sbjct: 592 KRLFSLINDLPTVFEVVT--ERKPIKDKPTVDS--GSKSRGGTKRSSDGQVKSSNPKFAD 759
Query: 61 DDEEVDDEEEYQGECTACGENY 82
+ E D++E + C +CG NY
Sbjct: 760 EGYEEDEDEHSETLCGSCGGNY 825
Score = 23.9 bits (50), Expect = 5.4
Identities = 10/33 (30%), Positives = 20/33 (60%)
Frame = -1
Query: 42 GSKARGSELAKYSKPPAKEDDEEVDDEEEYQGE 74
GS+ RG + A +DD++ DD+++ +G+
Sbjct: 214 GSRKRGEGA*EEEIADACDDDDDDDDDDDSEGK 116
>BP049886
Length = 541
Score = 61.2 bits (147), Expect = 3e-11
Identities = 30/40 (75%), Positives = 33/40 (82%)
Frame = +2
Query: 1 KRLFTPINDLPTIFEVVTGSAKKQTKEKPSVSSHNSNKSK 40
KRLF INDLPT+FEVVTG AKKQ KEK S S+H+SNK K
Sbjct: 422 KRLFGMINDLPTVFEVVTGMAKKQGKEKTSFSNHSSNKPK 541
>TC8118 similar to GB|AAN28771.1|23505823|AY143832 At3g11200/F11B9.12
{Arabidopsis thaliana;}, partial (89%)
Length = 1177
Score = 60.5 bits (145), Expect = 5e-11
Identities = 34/82 (41%), Positives = 48/82 (58%)
Frame = +2
Query: 1 KRLFTPINDLPTIFEVVTGSAKKQTKEKPSVSSHNSNKSKSGSKARGSELAKYSKPPAKE 60
KRLF+ IND PT+FEVVT +K K+KP+ S + ++ GS R S+ S P E
Sbjct: 515 KRLFSLINDFPTVFEVVTD--RKPAKDKPATDSGSKSR---GSTKRSSDGQVKSNPKFAE 679
Query: 61 DDEEVDDEEEYQGECTACGENY 82
+ E +D+E + C +CG NY
Sbjct: 680 EGYEEEDDEHSETLCGSCGGNY 745
>TC15130 similar to UP|O81488 (O81488) F9D12.13 protein, partial (26%)
Length = 583
Score = 58.5 bits (140), Expect = 2e-10
Identities = 34/59 (57%), Positives = 40/59 (67%)
Frame = +3
Query: 30 SVSSHNSNKSKSGSKARGSELAKYSKPPAKEDDEEVDDEEEYQGECTACGENYVSASDE 88
SVS+ SN +K+ SKA GSE KY K K++DEE DDEE + C ACGENY ASDE
Sbjct: 3 SVSNIASNNTKTSSKAGGSESVKYLK-QVKDEDEEEDDEEHGETLCGACGENY--ASDE 170
>TC18764 similar to UP|Q8LBJ5 (Q8LBJ5) Nucleic acid binding protein-like,
partial (35%)
Length = 848
Score = 47.8 bits (112), Expect = 3e-07
Identities = 35/82 (42%), Positives = 43/82 (51%), Gaps = 15/82 (18%)
Frame = +1
Query: 22 KKQTKEKPSVSSHNSNKSKSGSKARGSE--LAKYSKPPAKEDD-------------EEVD 66
K Q +K SH+SNK KS SK RGSE KYSK +D+ EE D
Sbjct: 55 KSQKDKKQGKESHSSNKPKSNSK-RGSEPQXVKYSKGLISKDEDEGEGEDEDEEGLEEED 231
Query: 67 DEEEYQGECTACGENYVSASDE 88
+EE + C ACGE+Y A+DE
Sbjct: 232 EEEHGETLCGACGESY--AADE 291
>TC10767 similar to UP|Q8LBJ5 (Q8LBJ5) Nucleic acid binding protein-like,
partial (54%)
Length = 648
Score = 43.1 bits (100), Expect = 9e-06
Identities = 20/24 (83%), Positives = 22/24 (91%)
Frame = +1
Query: 1 KRLFTPINDLPTIFEVVTGSAKKQ 24
KRLF IN+LPTIFEVVTG+AKKQ
Sbjct: 577 KRLFNMINELPTIFEVVTGAAKKQ 648
>TC10171 similar to UP|Q9M2B4 (Q9M2B4) Nucleic acid binding protein-like,
partial (26%)
Length = 506
Score = 30.8 bits (68), Expect = 0.044
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = +2
Query: 60 EDDEEVDDEEEYQGECTACGENY 82
+DD+E DD+E + C ACG++Y
Sbjct: 5 DDDDEEDDDEHGETLCGACGDHY 73
>TC14970 similar to UP|O24294 (O24294) Legumin (Minor small) precursor,
partial (51%)
Length = 1566
Score = 29.6 bits (65), Expect = 0.098
Identities = 20/73 (27%), Positives = 30/73 (40%)
Frame = +1
Query: 8 NDLPTIFEVVTGSAKKQTKEKPSVSSHNSNKSKSGSKARGSELAKYSKPPAKEDDEEVDD 67
+DL I + + E H + SG + R +P +ED++E +D
Sbjct: 472 DDLSFISPESADEDEDEEDEDEDQEQHGRHSRPSGRRTR--------RPSREEDEDEDED 627
Query: 68 EEEYQGECTACGE 80
EEE QG GE
Sbjct: 628 EEERQGGRRGPGE 666
>TC17533 similar to UP|Q9ZS85 (Q9ZS85) T4B21.1 protein, partial (11%)
Length = 596
Score = 29.3 bits (64), Expect = 0.13
Identities = 21/61 (34%), Positives = 30/61 (48%), Gaps = 6/61 (9%)
Frame = +2
Query: 28 KPSVSSHNS-NKSKSGSKARGSELAKYSKP----PAKEDDEEVD-DEEEYQGECTACGEN 81
KP +H + SG++A G+E A P K+DDEE+ +E Q C CGE
Sbjct: 11 KPHPLTHKPLTRRLSGAEALGTESAPVFLPNDTVEEKKDDEELAVPADEDQNTCALCGEP 190
Query: 82 Y 82
+
Sbjct: 191F 193
>AV418055
Length = 407
Score = 29.3 bits (64), Expect = 0.13
Identities = 23/77 (29%), Positives = 36/77 (45%), Gaps = 2/77 (2%)
Frame = -3
Query: 9 DLPTIFEVVTGSAKKQTKEKPSVSSHNSNKSKSGSK-ARGSELA-KYSKPPAKEDDEEVD 66
D PT F +A K V+ S ++ S S A G ++ + P +D+EE +
Sbjct: 384 DRPTSFAPAINAASVHPNFKT*VTEAESRRACSSSYLAPGFGVSWRRKSSPVDDDEEEEE 205
Query: 67 DEEEYQGECTACGENYV 83
+EEE + E T G + V
Sbjct: 204 EEEEEEVESTMIGVSVV 154
>TC12890 homologue to UP|Q40359 (Q40359) Alfin-1, partial (23%)
Length = 460
Score = 28.5 bits (62), Expect = 0.22
Identities = 13/23 (56%), Positives = 15/23 (64%)
Frame = +3
Query: 60 EDDEEVDDEEEYQGECTACGENY 82
E EE DD+E+ C ACGENY
Sbjct: 12 ESGEEEDDDEQ-GATCGACGENY 77
>AV418706
Length = 374
Score = 27.3 bits (59), Expect = 0.48
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = -1
Query: 58 AKEDDEEVDDEEEYQGECTA 77
A+EDDE+ +DE+E EC A
Sbjct: 218 AEEDDEDEEDEDEPAEECAA 159
>TC16074 similar to UP|Q8H9E8 (Q8H9E8) Resistant specific protein-3, partial
(6%)
Length = 844
Score = 27.3 bits (59), Expect = 0.48
Identities = 13/29 (44%), Positives = 19/29 (64%)
Frame = +2
Query: 44 KARGSELAKYSKPPAKEDDEEVDDEEEYQ 72
+ G EL K KP K+DD+ DDE+E++
Sbjct: 218 EGNGVELNK--KPVQKDDDDGADDEDEHK 298
>TC8321 similar to UP|AAS46230 (AAS46230) Peroxiredoxin Q, partial (75%)
Length = 873
Score = 27.3 bits (59), Expect = 0.48
Identities = 22/73 (30%), Positives = 38/73 (51%), Gaps = 6/73 (8%)
Frame = +1
Query: 8 NDLPTIFEVVTGSAKKQTKEKPSVSSHNSNKSKSGSKARGSELAKY---SKPPA---KED 61
++LP F + +++ + S SSH+S+ S S RGS +AK SKPP K+
Sbjct: 70 SNLPFPFPQSSSNSQFIGLKLSSSSSHSSSFSVPSSSFRGSIVAKVTTGSKPPGFTLKDQ 249
Query: 62 DEEVDDEEEYQGE 74
D + +++G+
Sbjct: 250 DGKTVSLSKFKGK 288
>TC10025 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein
(Fragment), partial (15%)
Length = 672
Score = 27.3 bits (59), Expect = 0.48
Identities = 15/49 (30%), Positives = 22/49 (44%)
Frame = +1
Query: 22 KKQTKEKPSVSSHNSNKSKSGSKARGSELAKYSKPPAKEDDEEVDDEEE 70
K+ K S + K K ++ G E K PP ++ +V DEEE
Sbjct: 232 KRLKKMSSDFESKKTGKEKEEDESAGHEDDKPVVPPKRKRSSKVKDEEE 378
>TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly protein
1-like protein 3, partial (81%)
Length = 1298
Score = 26.9 bits (58), Expect = 0.63
Identities = 17/53 (32%), Positives = 26/53 (48%), Gaps = 2/53 (3%)
Frame = +2
Query: 22 KKQTKEKPSVSSHNSNKSKSGSKARGSELAKYSKPPAKEDDEEVDDE--EEYQ 72
+K K+KP S N+ K S ++ P EDD+++DD+ EE Q
Sbjct: 506 QKILKKKPKKGSKNTKPITKTEKCE-SFFNFFNPPQVPEDDDDIDDDAVEELQ 661
Score = 23.9 bits (50), Expect = 5.4
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +2
Query: 37 NKSKSGSKARGSELAKYSKPP 57
+KSKSGSKAR + +PP
Sbjct: 884 SKSKSGSKARPGKDQPTERPP 946
>CN825457
Length = 537
Score = 26.6 bits (57), Expect = 0.83
Identities = 17/50 (34%), Positives = 20/50 (40%)
Frame = -3
Query: 36 SNKSKSGSKARGSELAKYSKPPAKEDDEEVDDEEEYQGECTACGENYVSA 85
S K S AR E+ P KE + E G C C E+ VSA
Sbjct: 493 SKKQNKASTARPREVKLAVLAPRKEASDLHSSRTEGAGGCGRCREHAVSA 344
>AV775735
Length = 381
Score = 26.6 bits (57), Expect = 0.83
Identities = 14/36 (38%), Positives = 20/36 (54%), Gaps = 3/36 (8%)
Frame = -1
Query: 44 KARGSELAKYSKP---PAKEDDEEVDDEEEYQGECT 76
+A+GS + S P P DD+E DDE++ CT
Sbjct: 330 RAQGSAEEEGSDPVTSPKSHDDDEDDDEDDE*NHCT 223
>TC9642
Length = 657
Score = 26.6 bits (57), Expect = 0.83
Identities = 19/73 (26%), Positives = 35/73 (47%)
Frame = -3
Query: 15 EVVTGSAKKQTKEKPSVSSHNSNKSKSGSKARGSELAKYSKPPAKEDDEEVDDEEEYQGE 74
E T AK++TK ++++ + + K GS K + DD+E +DE+E + E
Sbjct: 391 ETKTPVAKRKTKGSTALNT-SPVWPELTVKGLGSGALKSTGAANDTDDDEDEDEDEDEDE 215
Query: 75 CTACGENYVSASD 87
+ G +V +
Sbjct: 214 ASEIGSCWVEVEE 176
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.299 0.121 0.324
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,281,090
Number of Sequences: 28460
Number of extensions: 12253
Number of successful extensions: 178
Number of sequences better than 10.0: 89
Number of HSP's better than 10.0 without gapping: 153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 168
length of query: 88
length of database: 4,897,600
effective HSP length: 64
effective length of query: 24
effective length of database: 3,076,160
effective search space: 73827840
effective search space used: 73827840
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (21.6 bits)
S2: 48 (23.1 bits)
Medicago: description of AC149574.9