BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149574.1 - phase: 0 /pseudo
(619 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV776921 171 3e-43
BP042979 151 3e-37
AV411197 118 3e-27
BG662257 110 5e-25
BI417718 106 1e-23
AV418183 96 2e-20
BG662269 57 2e-19
BP039737 88 5e-18
TC11425 similar to UP|Q9M1Q9 (Q9M1Q9) P-glycoprotein-like proeti... 80 1e-15
BP055390 75 4e-14
TC11559 similar to UP|Q9SXT3 (Q9SXT3) Multidrug resistance prote... 72 3e-13
AV413975 71 4e-13
TC13598 similar to UP|Q9M1Q9 (Q9M1Q9) P-glycoprotein-like proeti... 67 1e-11
AV777119 64 7e-11
AV768362 59 3e-09
AV767093 57 9e-09
AW720269 57 9e-09
AU089009 53 1e-07
TC19496 similar to UP|Q8LPQ6 (Q8LPQ6) At4g25450/M7J2_180, partia... 51 5e-07
AU089197 47 9e-06
>AV776921
Length = 435
Score = 171 bits (433), Expect = 3e-43
Identities = 86/141 (60%), Positives = 110/141 (77%)
Frame = +2
Query: 450 FIYPSRPNVVIFKDFNLIVPSGKSLALVGHSGSGKSSIISLILRFYDPTSGKVMIDGKDI 509
F YPSRP+V I L +PSGK +ALVG SGSGKS++ISLI RFY+P SG +++DG DI
Sbjct: 11 FSYPSRPDVEILNKLCLDIPSGKIVALVGGSGSGKSTVISLIERFYEPLSGDILLDGNDI 190
Query: 510 KKMNLKSLRKQIGLVQQEPALFATSIYKNILYGKEEASESEVIEAAKLADAHNFISALPE 569
+ ++LK LR+QIGLV QEPALFATSI +NILYGK+ A+ E+ A KL+DA +FI+ LPE
Sbjct: 191 RDLDLKWLRQQIGLVNQEPALFATSIKENILYGKDNATLEELKRAVKLSDAQSFINNLPE 370
Query: 570 GYSTKAGDRGVLLSGGQKQRV 590
T+ G+RG+ LSGGQKQR+
Sbjct: 371 RLETQVGERGIQLSGGQKQRI 433
>BP042979
Length = 513
Score = 151 bits (381), Expect = 3e-37
Identities = 74/169 (43%), Positives = 114/169 (66%), Gaps = 3/169 (1%)
Frame = +2
Query: 285 EKLFMQGFGGNLSKAYLKANMLAGEAVSNIRTVAAFCAEEKVIDLYADELVEPSKRSFKR 344
+K+FM GF G+L A++KA LAGEA++N+RTVAAF +E K++ L+ L P +R F +
Sbjct: 2 QKMFMTGFSGDLEAAHVKATQLAGEAIANVRTVAAFNSEAKIVGLFTSNLEAPLQRCFWK 181
Query: 345 GQIAGIFYGISQFFIFSSYGLALWYGSVLLEKELASFKSIMKSFMVLIVTALAMGETLAL 404
GQI+G YGI+QF +++SY L LWY S L++ ++ F ++ FMVL+V+A ETL L
Sbjct: 182 GQISGSGYGIAQFALYASYALGLWYASWLVKHGISDFSKTIRVFMVLMVSANGAAETLTL 361
Query: 405 APDLLKGNQMVSSIFDMIDRKSGIIHDVGEELMT---VEGMIELKRINF 450
APD +KG + + S+FD++DR++ I D + + G +ELK ++F
Sbjct: 362 APDFIKGGRAMKSVFDLLDRRTEIEPDDQDATPVPDRLRGEVELKHVDF 508
>AV411197
Length = 391
Score = 118 bits (295), Expect = 3e-27
Identities = 57/97 (58%), Positives = 79/97 (80%)
Frame = +3
Query: 523 LVQQEPALFATSIYKNILYGKEEASESEVIEAAKLADAHNFISALPEGYSTKAGDRGVLL 582
L+QQEP +F+T+I +NI+Y + ASE+E+ EAA++A+AH+FIS+LP GY T G RGV L
Sbjct: 54 LIQQEPIIFSTTIRENIIYARHNASEAEMKEAARIANAHHFISSLPHGYDTHVGMRGVDL 233
Query: 583 SGGQKQRVAIARAILRNPKILLLDEATSALDVESERV 619
+ GQKQR+AIAR +L+N ILLLDEA+S+++ ES RV
Sbjct: 234 TPGQKQRIAIARVVLKNAPILLLDEASSSIESESSRV 344
>BG662257
Length = 336
Score = 110 bits (276), Expect = 5e-25
Identities = 55/78 (70%), Positives = 68/78 (86%)
Frame = +1
Query: 542 GKEEASESEVIEAAKLADAHNFISALPEGYSTKAGDRGVLLSGGQKQRVAIARAILRNPK 601
G + A+E+E+I AA+LA+AH FIS+L +GY T GDRG+ LSGGQKQRVAIARAI+++PK
Sbjct: 19 GGDAATEAEIIAAAELANAHKFISSLQQGYDTVVGDRGIQLSGGQKQRVAIARAIVKSPK 198
Query: 602 ILLLDEATSALDVESERV 619
ILLLDEATSALD ESE+V
Sbjct: 199 ILLLDEATSALDAESEKV 252
Score = 33.9 bits (76), Expect = 0.079
Identities = 14/19 (73%), Positives = 17/19 (88%)
Frame = +1
Query: 2 IGRTTVIVAHRLSTIKNAD 20
+ RTT++VAHRLSTIK AD
Sbjct: 280 VDRTTIVVAHRLSTIKGAD 336
>BI417718
Length = 515
Score = 106 bits (264), Expect = 1e-23
Identities = 60/165 (36%), Positives = 96/165 (57%), Gaps = 2/165 (1%)
Frame = +2
Query: 325 KVIDLYADELVEPSKRSFKRGQIAGIFYGISQFFIFSSYGLALWYGSVLLEKELASFKSI 384
+V +LY+ + P K +RG I+GI +G+S F +FS Y G+ + +ASF +
Sbjct: 29 RVGELYSRKCEGPVKTGIQRGLISGIGFGVSFFLLFSVYATTFHVGARFVGAGMASFSDV 208
Query: 385 MKSFMVLIVTALAMGETLALAPDLLKGNQMVSSIFDMIDRKSGI--IHDVGEELMTVEGM 442
+ L + A+ + AP+ K + +SIF++IDRKS I + G L + +G
Sbjct: 209 FQVLFALTMAAIGISRR---APNSSKAKIVTASIFEIIDRKSKIDPCDESGSTLDSTKGK 379
Query: 443 IELKRINFIYPSRPNVVIFKDFNLIVPSGKSLALVGHSGSGKSSI 487
IE ++F YPSRP++ IF D +L + +G ++ALVG SGSGKS++
Sbjct: 380 IEFCHVSFKYPSRPDIQIFPDLSLTIHAGTTVALVGESGSGKSTV 514
>AV418183
Length = 298
Score = 95.5 bits (236), Expect = 2e-20
Identities = 52/98 (53%), Positives = 68/98 (69%), Gaps = 1/98 (1%)
Frame = +3
Query: 506 GKDIKKMNLKSLRKQIGLVQQEPALFATSIYKNILYGKEEASESEVIE-AAKLADAHNFI 564
G + +++ + L ++I +V QEP LF SI +NI YG + S IE AAK+A+AH FI
Sbjct: 3 GVPLAEISHRHLHRKISIVSQEPTLFNCSIEENIAYGFDGKLNSVDIENAAKMANAHEFI 182
Query: 565 SALPEGYSTKAGDRGVLLSGGQKQRVAIARAILRNPKI 602
S PE Y T G+RGV LSGGQKQR+AIARA+L +PKI
Sbjct: 183 SKFPEKYQTFVGERGVRLSGGQKQRIAIARALLMDPKI 296
>BG662269
Length = 283
Score = 57.0 bits (136), Expect(2) = 2e-19
Identities = 29/56 (51%), Positives = 42/56 (74%), Gaps = 1/56 (1%)
Frame = +2
Query: 508 DIKKMNLKSLRKQIGLVQQEPALFATSIYKNILYGK-EEASESEVIEAAKLADAHN 562
+I+ + +K LR+Q+G+V QEP LF +I NI YGK A+E+E+I AA+LA+AHN
Sbjct: 17 EIQTLQVKWLRQQMGMVSQEPVLFNETIRANIAYGKGGNATEAEIIAAAELANAHN 184
Score = 56.2 bits (134), Expect(2) = 2e-19
Identities = 27/34 (79%), Positives = 31/34 (90%)
Frame = +3
Query: 573 TKAGDRGVLLSGGQKQRVAIARAILRNPKILLLD 606
T G+RG+ LSGGQKQRVAIARAI++NPKILLLD
Sbjct: 180 TIVGERGIQLSGGQKQRVAIARAIVKNPKILLLD 281
>BP039737
Length = 543
Score = 87.8 bits (216), Expect = 5e-18
Identities = 48/164 (29%), Positives = 83/164 (50%), Gaps = 2/164 (1%)
Frame = +3
Query: 317 VAAFCAEEKVIDLYADELVEPSKRSFKRGQIAGIFYGISQFFIFSSYGLALWYGSVLLEK 376
V ++ E K ++ Y+D + K +K G G+ G + S+ L WY V +
Sbjct: 51 VYSYVGESKALNSYSDAIQNTLKLGYKAGMAKGLGLGCTYGIACMSWALVFWYAGVFIRN 230
Query: 377 ELASFKSIMKSFMVLIVTALAMGETLALAPDLLKGNQMVSSIFDMIDRKSGIIHDV--GE 434
+ IV +++G++ + KG + ++I +K II D+ G+
Sbjct: 231 GQTDGGKAFTAIFSAIVGGMSLGQSFSNLGAFSKGKAAGYKLMEIIKQKPTIIEDLSDGK 410
Query: 435 ELMTVEGMIELKRINFIYPSRPNVVIFKDFNLIVPSGKSLALVG 478
L V G IE K + F YPSRP+V+IF++F++ P+GK++A+VG
Sbjct: 411 CLDEVNGNIEFKDVTFSYPSRPDVIIFRNFSIFFPAGKTVAVVG 542
>TC11425 similar to UP|Q9M1Q9 (Q9M1Q9) P-glycoprotein-like proetin, partial
(9%)
Length = 712
Score = 80.1 bits (196), Expect = 1e-15
Identities = 40/50 (80%), Positives = 43/50 (86%)
Frame = +2
Query: 570 GYSTKAGDRGVLLSGGQKQRVAIARAILRNPKILLLDEATSALDVESERV 619
GY T G+RG LLSGGQKQRVAIARAI++ P ILLLDEATSALD ESERV
Sbjct: 71 GYDTVVGERGTLLSGGQKQRVAIARAIIKTPNILLLDEATSALDAESERV 220
Score = 67.8 bits (164), Expect = 5e-12
Identities = 33/52 (63%), Positives = 41/52 (78%)
Frame = +2
Query: 1 MIGRTTVIVAHRLSTIKNADMIAVVEGGSVVETGNHEELISNPNSLYSSLVQ 52
M+ RTTVIVAHRLSTIKNAD+I V++ G +VE G HE LI+ + Y+SLVQ
Sbjct: 245 MVNRTTVIVAHRLSTIKNADVITVLKNGVIVEKGRHETLINIKDGYYASLVQ 400
>BP055390
Length = 488
Score = 74.7 bits (182), Expect = 4e-14
Identities = 37/47 (78%), Positives = 42/47 (88%)
Frame = +3
Query: 573 TKAGDRGVLLSGGQKQRVAIARAILRNPKILLLDEATSALDVESERV 619
T+ G RGV LSGGQKQR+AIARAIL+NP ILLLDEATSALD ESE++
Sbjct: 204 TQVGQRGVQLSGGQKQRIAIARAILKNPPILLLDEATSALDTESEKL 344
Score = 57.8 bits (138), Expect = 5e-09
Identities = 26/40 (65%), Positives = 32/40 (80%)
Frame = +3
Query: 1 MIGRTTVIVAHRLSTIKNADMIAVVEGGSVVETGNHEELI 40
M GRT +++AHRLST+ NAD+IAVVE G VVETG H L+
Sbjct: 369 MHGRTVILIAHRLSTVVNADVIAVVENGQVVETGTHHTLL 488
>TC11559 similar to UP|Q9SXT3 (Q9SXT3) Multidrug resistance protein
(Fragment), partial (41%)
Length = 460
Score = 72.0 bits (175), Expect = 3e-13
Identities = 37/110 (33%), Positives = 67/110 (60%), Gaps = 4/110 (3%)
Frame = +3
Query: 454 SRPNVVIFKDFNLIVPSGKSLALVGHSGSGKSSIISLILRFYDPTSGKVMIDGKDIKKMN 513
S V I K +L +P G + ++G SGSGKS+++ + R ++P S V +D +DI ++
Sbjct: 117 SDDGVPILKGIHLDIPKGVIVGVIGPSGSGKSTLLRALNRLWEPPSASVFLDARDICHLD 296
Query: 514 LKSLRKQIGLVQQEPALFATSIYKNILYGK----EEASESEVIEAAKLAD 559
+ SLR+++G++ Q PALF ++ N+ YG ++ ++ EV + +AD
Sbjct: 297 VLSLRRKVGMLFQLPALFEGTVADNVRYGPQLRGQKLTDDEVRKLLIMAD 446
>AV413975
Length = 343
Score = 71.2 bits (173), Expect = 4e-13
Identities = 38/94 (40%), Positives = 59/94 (62%), Gaps = 5/94 (5%)
Frame = +2
Query: 418 IFDMIDR-----KSGIIHDVGEELMTVEGMIELKRINFIYPSRPNVVIFKDFNLIVPSGK 472
+F ++DR KSG +G++ +G +EL + F YPSRP+ + K + + G
Sbjct: 71 VFQIMDRVSSMSKSGTKCPLGDQ----DGEVELDDVWFSYPSRPSHPVLKGITMKLHPGS 238
Query: 473 SLALVGHSGSGKSSIISLILRFYDPTSGKVMIDG 506
+ALVG SG GK++I +LI RFYDPT GK++++G
Sbjct: 239 KVALVGPSGGGKTTIANLIERFYDPTKGKILLNG 340
>TC13598 similar to UP|Q9M1Q9 (Q9M1Q9) P-glycoprotein-like proetin, partial
(7%)
Length = 552
Score = 66.6 bits (161), Expect = 1e-11
Identities = 33/52 (63%), Positives = 41/52 (78%)
Frame = +3
Query: 1 MIGRTTVIVAHRLSTIKNADMIAVVEGGSVVETGNHEELISNPNSLYSSLVQ 52
M+ RTTVIVAHRLSTIK+AD+I V++ G +VE G HE LIS + Y+SLVQ
Sbjct: 96 MVNRTTVIVAHRLSTIKSADVIIVLKNGVIVEKGRHETLISIKDGYYASLVQ 251
Score = 38.9 bits (89), Expect = 0.002
Identities = 18/23 (78%), Positives = 21/23 (91%)
Frame = +3
Query: 597 LRNPKILLLDEATSALDVESERV 619
+++P ILLLDE TSALDVESERV
Sbjct: 3 IKSPNILLLDEXTSALDVESERV 71
>AV777119
Length = 447
Score = 63.9 bits (154), Expect = 7e-11
Identities = 32/58 (55%), Positives = 43/58 (73%), Gaps = 1/58 (1%)
Frame = -1
Query: 1 MIGRTTVIVAHRLSTIKNADMIAVVEGGSVVETGNHEELIS-NPNSLYSSLVQGQPSP 57
M+GRT+V+VAHRLSTI+N D+IAV++ G VVE G+H L++ P+ Y SLV Q P
Sbjct: 444 MVGRTSVVVAHRLSTIQNCDLIAVLDKGRVVEKGSHSNLLAKGPSGAYYSLVSLQRRP 271
>AV768362
Length = 541
Score = 58.5 bits (140), Expect = 3e-09
Identities = 27/54 (50%), Positives = 39/54 (72%)
Frame = -2
Query: 1 MIGRTTVIVAHRLSTIKNADMIAVVEGGSVVETGNHEELISNPNSLYSSLVQGQ 54
M GRT +++AHRLST+K A+ + V+ G V E G H+EL+S N +YS+LV+ Q
Sbjct: 498 MKGRTVLVIAHRLSTVKTANTVVVISDGQVAEKGTHDELLSQ-NGVYSALVKRQ 340
>AV767093
Length = 603
Score = 57.0 bits (136), Expect = 9e-09
Identities = 29/75 (38%), Positives = 48/75 (63%), Gaps = 1/75 (1%)
Frame = +1
Query: 441 GMIELKRINFIYPSRPNV-VIFKDFNLIVPSGKSLALVGHSGSGKSSIISLILRFYDPTS 499
G IEL + Y RPN ++ K +L V G+ + +VG +GSGKS++I ++ R +P++
Sbjct: 379 GSIELNSLQVRY--RPNTPLVLKGISLTVQGGEKIGVVGRTGSGKSTLIQVLFRLIEPSA 552
Query: 500 GKVMIDGKDIKKMNL 514
GK++IDG +I + L
Sbjct: 553 GKIIIDGINICTLGL 597
>AW720269
Length = 524
Score = 57.0 bits (136), Expect = 9e-09
Identities = 49/165 (29%), Positives = 77/165 (45%), Gaps = 10/165 (6%)
Frame = +3
Query: 458 VVIFKDFNLIVPSGKSLALVGHSGSGKSSIISLIL-----RFYDPTSGKVMIDGKDIKKM 512
V I K + + S + +A+VG SG+GKS+++ +I R +DP + I D
Sbjct: 48 VNILKSVSFVARSSEIVAVVGPSGTGKSTLLRIIAGRVKDRDFDPKT----ISINDHPMT 215
Query: 513 NLKSLRKQIGLVQQEPALFATSIYKNILYGKEEASESEVIEAAKLADAHNFISAL----- 567
+ LRK G V QE L K L + E+ + + + L
Sbjct: 216 SPAQLRKICGFVAQEDNLLPLLTVKETLLFSAKFRLKEMTPNDREMRVESLMQELGLFHV 395
Query: 568 PEGYSTKAGDRGVLLSGGQKQRVAIARAILRNPKILLLDEATSAL 612
+ + +RG+ SGG+++RV+I ++ NP ILLLDE TS L
Sbjct: 396 SDSFVGDEENRGI--SGGERKRVSIGVDMIHNPPILLLDEPTSGL 524
>AU089009
Length = 209
Score = 53.1 bits (126), Expect = 1e-07
Identities = 25/49 (51%), Positives = 34/49 (69%)
Frame = +1
Query: 568 PEGYSTKAGDRGVLLSGGQKQRVAIARAILRNPKILLLDEATSALDVES 616
P G T+ G+RGV LSGGQKQRV +ARA+ +N + LLD+ A+D +
Sbjct: 7 PHGDLTEIGERGVNLSGGQKQRVQLARALYQNADVYLLDDPXXAVDAHT 153
>TC19496 similar to UP|Q8LPQ6 (Q8LPQ6) At4g25450/M7J2_180, partial (10%)
Length = 571
Score = 51.2 bits (121), Expect = 5e-07
Identities = 26/54 (48%), Positives = 36/54 (66%)
Frame = +3
Query: 1 MIGRTTVIVAHRLSTIKNADMIAVVEGGSVVETGNHEELISNPNSLYSSLVQGQ 54
M GRTT+++AHRLST++NA IA+ G + E G H EL++ Y+SLV Q
Sbjct: 48 MKGRTTLVIAHRLSTVQNAYQIALCSDGRIAEQGTHFELLAKKGQ-YASLVGTQ 206
>AU089197
Length = 658
Score = 47.0 bits (110), Expect = 9e-06
Identities = 25/77 (32%), Positives = 42/77 (54%), Gaps = 1/77 (1%)
Frame = +1
Query: 440 EGMIELKRINFIY-PSRPNVVIFKDFNLIVPSGKSLALVGHSGSGKSSIISLILRFYDPT 498
+GMIEL ++ Y P+ P ++ KD I K + + +GSGKS+++ + P
Sbjct: 349 DGMIELHKLQVQYDPAAP--IVLKDVTFIFSGQKKIGIXXRTGSGKSTLLQALF*IVXPL 522
Query: 499 SGKVMIDGKDIKKMNLK 515
SG ++IDG I ++ K
Sbjct: 523 SGCILIDGVXIPRLACK 573
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.136 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,593,876
Number of Sequences: 28460
Number of extensions: 102813
Number of successful extensions: 650
Number of sequences better than 10.0: 63
Number of HSP's better than 10.0 without gapping: 639
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 646
length of query: 619
length of database: 4,897,600
effective HSP length: 96
effective length of query: 523
effective length of database: 2,165,440
effective search space: 1132525120
effective search space used: 1132525120
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC149574.1