BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149547.6 + phase: 0
(160 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC17523 similar to UP|Q9FKX6 (Q9FKX6) Prolyl 4-hydroxylase, alph... 193 1e-50
TC15863 161 4e-41
TC9187 similar to UP|Q8LAN3 (Q8LAN3) Prolyl 4-hydroxylase alpha ... 155 2e-39
BP069281 122 2e-29
TC11925 similar to UP|Q9FKX6 (Q9FKX6) Prolyl 4-hydroxylase, alph... 117 7e-28
TC13277 similar to UP|Q9LSI6 (Q9LSI6) Prolyl 4-hydroxylase alpha... 92 3e-20
AV766573 83 3e-17
TC17036 similar to UP|Q8VZD7 (Q8VZD7) AT3g28480/MFJ20_16, partia... 75 7e-15
BP061999 70 1e-13
TC19415 similar to UP|BAD07294 (BAD07294) Prolyl 4-hydroxylase, ... 69 3e-13
TC16820 similar to UP|Q8L8T9 (Q8L8T9) Prolyl 4-hydroxylase alpha... 42 2e-11
BP038912 31 0.12
TC13810 29 0.34
TC8150 similar to UP|AAR88248 (AAR88248) Mitochondrial citrate s... 26 3.7
TC15237 similar to UP|AAQ24632 (AAQ24632) GPRP, partial (64%) 25 4.9
TC14691 25 4.9
TC10456 homologue to UP|O23207 (O23207) Minor allergen, partial ... 25 6.4
TC8691 similar to UP|Q940T5 (Q940T5) AT5g59550/f2o15_210, partia... 25 6.4
BP059690 25 8.3
>TC17523 similar to UP|Q9FKX6 (Q9FKX6) Prolyl 4-hydroxylase, alpha
subunit-like protein, partial (55%)
Length = 448
Score = 193 bits (490), Expect = 1e-50
Identities = 93/145 (64%), Positives = 106/145 (72%), Gaps = 21/145 (14%)
Frame = +1
Query: 4 STVDDETGKSVDNSARTSSGTFINRGHDKILRNIEQRIADFTFIPVENGESVNILHYEVG 63
+ VD ETGKS D+ RTSSGTF+ RG DKI+RNIE++IADFTFIPVE+GE + +LHYEVG
Sbjct: 13 TVVDSETGKSKDSRVRTSSGTFLPRGRDKIVRNIEKKIADFTFIPVEHGEGLQVLHYEVG 192
Query: 64 QKYEPHPDFFTDEINTKNGGERVATMLMYL---------------------PWWNELSDC 102
QKYEPH D+F DE NTKNGG+R+AT+LMYL PWWNELSDC
Sbjct: 193 QKYEPHYDYFLDEFNTKNGGQRIATVLMYLTDVEXGGETVFPSAKGNFXSVPWWNELSDC 372
Query: 103 GKKGLSIKPKMGDALLFWSMKPDGT 127
GKKGLSI P GDALLFWSM P T
Sbjct: 373 GKKGLSINPXXGDALLFWSMNPXAT 447
>TC15863
Length = 607
Score = 161 bits (408), Expect = 4e-41
Identities = 72/125 (57%), Positives = 90/125 (71%), Gaps = 21/125 (16%)
Frame = +2
Query: 55 VNILHYEVGQKYEPHPDFFTDEINTKNGGERVATMLMYL--------------------- 93
+ +LHYEVGQKYEPH D+F DE NTKNGG+R+AT+LMYL
Sbjct: 5 LQVLHYEVGQKYEPHYDYFLDEFNTKNGGQRIATVLMYLSDVEEGGETIFPAAKANFSSV 184
Query: 94 PWWNELSDCGKKGLSIKPKMGDALLFWSMKPDGTLDPLSMHGACPVIKGDKWSCTKWMRV 153
PW+N+LS C KKGLS+KPK GDALLFWS++PD TLDP S+HG CPVI+G+KWS TKWM +
Sbjct: 185 PWYNDLSVCAKKGLSVKPKRGDALLFWSIRPDATLDPSSLHGGCPVIRGNKWSSTKWMHL 364
Query: 154 GKWSI 158
++ +
Sbjct: 365 EEYKV 379
>TC9187 similar to UP|Q8LAN3 (Q8LAN3) Prolyl 4-hydroxylase alpha
subunit-like protein, partial (89%)
Length = 1198
Score = 155 bits (393), Expect = 2e-39
Identities = 76/173 (43%), Positives = 109/173 (62%), Gaps = 23/173 (13%)
Frame = +2
Query: 4 STVDDETGKSVDNSARTSSGTFINRGHDKILRNIEQRIADFTFIPVENGESVNILHYEVG 63
+ D+ +G S + RTSSG FI++ D I+ IE +I+ +TF+P ENGE + +L YE G
Sbjct: 278 AVADNLSGDSKLSEVRTSSGMFISKKKDPIVAGIEDKISAWTFLPKENGEDMQVLRYEHG 457
Query: 64 QKYEPHPDFFTDEINTKNGGERVATMLMYLP-----------------------WWNELS 100
QKY+PH D+FTD++N GG R+AT+L+YL ++LS
Sbjct: 458 QKYDPHYDYFTDKVNIVRGGHRMATVLLYLTNVTRGGETVFPVAEEPPRRRGLETNSDLS 637
Query: 101 DCGKKGLSIKPKMGDALLFWSMKPDGTLDPLSMHGACPVIKGDKWSCTKWMRV 153
+C KKG+++KP+ GDALLF+S+ T DP S+H CPVI+G+KWS TKW+ V
Sbjct: 638 ECAKKGIAVKPRRGDALLFFSLHTTATPDPDSLHAGCPVIEGEKWSATKWIHV 796
>BP069281
Length = 468
Score = 122 bits (307), Expect = 2e-29
Identities = 51/64 (79%), Positives = 56/64 (86%)
Frame = -3
Query: 93 LPWWNELSDCGKKGLSIKPKMGDALLFWSMKPDGTLDPLSMHGACPVIKGDKWSCTKWMR 152
+PWWNELSDCGKKGLSIKPK GDALLFWSMKPD T D S+HG CPV KGDKWSCTKWMR
Sbjct: 460 VPWWNELSDCGKKGLSIKPKRGDALLFWSMKPDTTADTSSLHGGCPVTKGDKWSCTKWMR 281
Query: 153 VGKW 156
+ ++
Sbjct: 280 LNEY 269
>TC11925 similar to UP|Q9FKX6 (Q9FKX6) Prolyl 4-hydroxylase, alpha
subunit-like protein, partial (43%)
Length = 613
Score = 117 bits (294), Expect = 7e-28
Identities = 56/80 (70%), Positives = 67/80 (83%), Gaps = 1/80 (1%)
Frame = +1
Query: 1 MHKSTV-DDETGKSVDNSARTSSGTFINRGHDKILRNIEQRIADFTFIPVENGESVNILH 59
M KSTV D ETGKS D+ ARTSSGTF+ RG DKI+RNIE+RIAD+TFIP+E+GE + +LH
Sbjct: 373 MRKSTVVDSETGKSKDSRARTSSGTFLARGRDKIVRNIEKRIADYTFIPIEHGEGLQVLH 552
Query: 60 YEVGQKYEPHPDFFTDEINT 79
YEVGQKY+ H D+F DE NT
Sbjct: 553 YEVGQKYDSHYDYFMDEFNT 612
>TC13277 similar to UP|Q9LSI6 (Q9LSI6) Prolyl 4-hydroxylase alpha
subunit-like protein, partial (34%)
Length = 582
Score = 92.4 bits (228), Expect = 3e-20
Identities = 39/66 (59%), Positives = 54/66 (81%)
Frame = +1
Query: 7 DDETGKSVDNSARTSSGTFINRGHDKILRNIEQRIADFTFIPVENGESVNILHYEVGQKY 66
D+E+GKS+ + RTSSG F+N+ D+++ +IE RIA +TF+P+ENGES+ ILHYE GQKY
Sbjct: 385 DNESGKSIKSEVRTSSGMFLNKAQDEVVADIEARIATWTFLPIENGESIQILHYENGQKY 564
Query: 67 EPHPDF 72
EPH D+
Sbjct: 565 EPHYDY 582
>AV766573
Length = 338
Score = 82.8 bits (203), Expect = 3e-17
Identities = 32/50 (64%), Positives = 42/50 (84%)
Frame = -3
Query: 109 IKPKMGDALLFWSMKPDGTLDPLSMHGACPVIKGDKWSCTKWMRVGKWSI 158
+KPK GDALLFWS++PD TLDP S+HG CPVI+G+KWS TKWM + ++ +
Sbjct: 336 VKPKRGDALLFWSIRPDATLDPSSLHGGCPVIRGNKWSSTKWMHLEEYKV 187
>TC17036 similar to UP|Q8VZD7 (Q8VZD7) AT3g28480/MFJ20_16, partial (21%)
Length = 697
Score = 74.7 bits (182), Expect = 7e-15
Identities = 35/75 (46%), Positives = 49/75 (64%), Gaps = 3/75 (4%)
Frame = +2
Query: 82 GGERV---ATMLMYLPWWNELSDCGKKGLSIKPKMGDALLFWSMKPDGTLDPLSMHGACP 138
GGE + A + P S+C KG ++KP+ GDALLF+S+ + T D S+HG+CP
Sbjct: 11 GGETIFPHAESKLSQPKDESWSECAHKGYAVKPRKGDALLFFSLHLNATTDSNSLHGSCP 190
Query: 139 VIKGDKWSCTKWMRV 153
VI+G+KWS TKW+ V
Sbjct: 191 VIEGEKWSATKWIHV 235
>BP061999
Length = 564
Score = 70.5 bits (171), Expect = 1e-13
Identities = 28/35 (80%), Positives = 30/35 (85%)
Frame = -2
Query: 119 FWSMKPDGTLDPLSMHGACPVIKGDKWSCTKWMRV 153
FWSMKPD T D S+HG CPVIKGDKWSCTKWMR+
Sbjct: 563 FWSMKPDTTADTSSLHGGCPVIKGDKWSCTKWMRL 459
>TC19415 similar to UP|BAD07294 (BAD07294) Prolyl 4-hydroxylase, partial
(24%)
Length = 443
Score = 69.3 bits (168), Expect = 3e-13
Identities = 27/47 (57%), Positives = 38/47 (80%)
Frame = +3
Query: 106 GLSIKPKMGDALLFWSMKPDGTLDPLSMHGACPVIKGDKWSCTKWMR 152
GL ++P+ GD LLF+S+ P+GT+DP S+ G+CPVIKG+KW KW+R
Sbjct: 51 GLRVRPRPGDGLLFYSLFPNGTIDPTSLPGSCPVIKGEKWVAPKWIR 191
>TC16820 similar to UP|Q8L8T9 (Q8L8T9) Prolyl 4-hydroxylase alpha
subunit-like protein, partial (7%)
Length = 571
Score = 42.4 bits (98), Expect(2) = 2e-11
Identities = 15/23 (65%), Positives = 18/23 (78%)
Frame = +3
Query: 130 PLSMHGACPVIKGDKWSCTKWMR 152
P S+HG C V+ G+KWS TKWMR
Sbjct: 363 PFSVHGGCEVLAGEKWSATKWMR 431
Score = 40.8 bits (94), Expect(2) = 2e-11
Identities = 20/33 (60%), Positives = 23/33 (69%), Gaps = 3/33 (9%)
Frame = +2
Query: 102 CGKK---GLSIKPKMGDALLFWSMKPDGTLDPL 131
CG K GLS+KP G+A+LFWSM DG DPL
Sbjct: 269 CGGKTVEGLSVKPTKGNAVLFWSMGLDGQSDPL 367
>BP038912
Length = 481
Score = 30.8 bits (68), Expect = 0.12
Identities = 11/19 (57%), Positives = 13/19 (67%)
Frame = -2
Query: 132 SMHGACPVIKGDKWSCTKW 150
S H CPV+KGD WS K+
Sbjct: 477 SYHARCPVLKGDMWSAIKF 421
>TC13810
Length = 486
Score = 29.3 bits (64), Expect = 0.34
Identities = 19/71 (26%), Positives = 33/71 (45%)
Frame = -3
Query: 11 GKSVDNSARTSSGTFINRGHDKILRNIEQRIADFTFIPVENGESVNILHYEVGQKYEPHP 70
G + N + IN G ++++R+ Q + F+ +P G L Y PHP
Sbjct: 364 GDGIFNYFKKQKKDTINSGQERMMRHHTQNLFKFSLVPRSFG-----LWY---LNQCPHP 209
Query: 71 DFFTDEINTKN 81
FF++ I++ N
Sbjct: 208 AFFSNMISSSN 176
>TC8150 similar to UP|AAR88248 (AAR88248) Mitochondrial citrate synthase,
partial (98%)
Length = 1956
Score = 25.8 bits (55), Expect = 3.7
Identities = 12/27 (44%), Positives = 17/27 (62%), Gaps = 1/27 (3%)
Frame = +3
Query: 92 YLPWWNELSDC-GKKGLSIKPKMGDAL 117
Y WWNE +DC G LS P+ G+++
Sbjct: 276 YGTWWNERNDCFGVAWLSC*PR*GNSV 356
>TC15237 similar to UP|AAQ24632 (AAQ24632) GPRP, partial (64%)
Length = 814
Score = 25.4 bits (54), Expect = 4.9
Identities = 7/13 (53%), Positives = 9/13 (68%)
Frame = -1
Query: 147 CTKWMRVGKWSIP 159
CT+W R+ W IP
Sbjct: 361 CTRWTRITSWGIP 323
>TC14691
Length = 1255
Score = 25.4 bits (54), Expect = 4.9
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = -1
Query: 18 ARTSSGTFINRGHDKILRNIEQR 40
AR+S F +G D RNIEQR
Sbjct: 616 ARSSRNIFHRKGQDNQRRNIEQR 548
>TC10456 homologue to UP|O23207 (O23207) Minor allergen, partial (27%)
Length = 463
Score = 25.0 bits (53), Expect = 6.4
Identities = 7/10 (70%), Positives = 9/10 (90%)
Frame = -1
Query: 93 LPWWNELSDC 102
+PWW +LSDC
Sbjct: 40 IPWWCQLSDC 11
>TC8691 similar to UP|Q940T5 (Q940T5) AT5g59550/f2o15_210, partial (16%)
Length = 942
Score = 25.0 bits (53), Expect = 6.4
Identities = 8/14 (57%), Positives = 8/14 (57%)
Frame = +1
Query: 95 WWNELSDCGKKGLS 108
WW E C KKG S
Sbjct: 220 WWGEFKPCTKKGFS 261
>BP059690
Length = 335
Score = 24.6 bits (52), Expect = 8.3
Identities = 7/15 (46%), Positives = 10/15 (66%)
Frame = +2
Query: 88 TMLMYLPWWNELSDC 102
T L Y+PW N++ C
Sbjct: 68 TFLFYIPWLNQMGKC 112
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.136 0.438
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,259,571
Number of Sequences: 28460
Number of extensions: 46069
Number of successful extensions: 233
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 230
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 231
length of query: 160
length of database: 4,897,600
effective HSP length: 83
effective length of query: 77
effective length of database: 2,535,420
effective search space: 195227340
effective search space used: 195227340
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)
Medicago: description of AC149547.6