BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149496.5 + phase: 0
(1716 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP066094 231 3e-66
BP084332 145 7e-35
AV780009 110 1e-24
BP052632 104 1e-22
BP075943 79 6e-15
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 50 1e-12
BP063694 65 7e-11
AV766665 63 4e-10
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 60 3e-09
AV427921 59 6e-09
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 58 1e-08
AU088980 55 7e-08
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 54 2e-07
BP052756 53 5e-07
BP057233 50 4e-06
AV424544 49 7e-06
BP033602 48 1e-05
BP043850 44 2e-04
TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%) 44 3e-04
AV766088 43 4e-04
>BP066094
Length = 532
Score = 231 bits (590), Expect(3) = 3e-66
Identities = 112/149 (75%), Positives = 132/149 (88%)
Frame = +2
Query: 1294 ARLEAIRLLLSYAINHGIILYQMDVKSAFLNGVIEEEVYVKQPPGFEDIKHPDHVYKLKK 1353
++ EAIRLL+S+++NH IIL+QM+VKSAFLNG I EEVYV QPPG ED K+ DH++KLKK
Sbjct: 86 SKTEAIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPGXEDEKNSDHIFKLKK 265
Query: 1354 SLYGLKQAPRAWYDRLSNFLIKNDFERGQVDTTLFGKTLKKDILIVQIYVDDIIFGSTNA 1413
SLYGLKQAPRAWY+RLS+FL++N+ RG+VDTTLF KT K DILIVQIYVDDIIFGS N
Sbjct: 266 SLYGLKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANP 445
Query: 1414 SLCKEFSKLMQDEFEMSMMGELKFFLGIQ 1442
SLCKEFS++MQ EFEM MMGELK+FLGIQ
Sbjct: 446 SLCKEFSEMMQAEFEMRMMGELKYFLGIQ 532
Score = 37.4 bits (85), Expect(3) = 3e-66
Identities = 17/18 (94%), Positives = 17/18 (94%)
Frame = +3
Query: 1278 YSQQEGIDYTETFAPVAR 1295
YSQQ GIDYTETFAPVAR
Sbjct: 39 YSQQ*GIDYTETFAPVAR 92
Score = 23.1 bits (48), Expect(3) = 3e-66
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +1
Query: 1268 RNKARLVAQG 1277
RNKARLVAQG
Sbjct: 10 RNKARLVAQG 39
>BP084332
Length = 368
Score = 145 bits (365), Expect = 7e-35
Identities = 68/81 (83%), Positives = 76/81 (92%)
Frame = +2
Query: 1598 QATIAMSTAEAEYISAASCCTQLLWMKHQLEDYQINANSIPIYCDNTAAICLSKNPILHS 1657
Q+TIA+STAEAEYISAA C TQ+LWMKHQLEDYQI ++IPIYCDNTAAI LSKNPILHS
Sbjct: 32 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILESNIPIYCDNTAAISLSKNPILHS 211
Query: 1658 RAKHIEIKHHFIRDYVQKGIL 1678
RAKHIE+K+HFIRDYVQKG+L
Sbjct: 212 RAKHIEVKYHFIRDYVQKGVL 274
>AV780009
Length = 529
Score = 110 bits (276), Expect = 1e-24
Identities = 52/145 (35%), Positives = 88/145 (59%), Gaps = 1/145 (0%)
Frame = -1
Query: 1535 AVKRIFRYLKGTTNLGLLYRKSLDYKLIGFCDADYAGDRIERKSTSGNCQFLGENLISWA 1594
A R+ RY+KG GL + KL + D+D+AG R+S +G FLG +LISW
Sbjct: 526 AATRVLRYVKGAPAQGLFFSADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSLISWR 347
Query: 1595 SKRQATIAMSTAEAEYISAASCCTQLLWMKHQLEDYQINAN-SIPIYCDNTAAICLSKNP 1653
+K+Q T++ S++EAEY + A+ ++ W+ + + ++N +P++CDN +A+ ++ NP
Sbjct: 346 TKKQTTVSRSSSEAEYRALAATVCEVQWLSYLFQFLKLNVPLPVPLFCDNQSALHIAHNP 167
Query: 1654 ILHSRAKHIEIKHHFIRDYVQKGIL 1678
H R KHIE+ H +R +Q G++
Sbjct: 166 TFHERTKHIELDCHVVRAKLQAGLI 92
>BP052632
Length = 489
Score = 104 bits (259), Expect = 1e-22
Identities = 56/120 (46%), Positives = 74/120 (61%), Gaps = 2/120 (1%)
Frame = -3
Query: 1012 KTAYELFKGRRPNISYFHQFGCTCYILNTKDYLKKFDAKAQRGIFLGYSERSKAYRVYNS 1071
KT YEL++GRRP +SYFH FGC +ILNT D + KFD K + I LGYS SKAY V+NS
Sbjct: 487 KTPYELWRGRRPTVSYFHPFGCK*FILNTNDNIGKFDNKFDKRILLGYSSNSKAYIVFNS 308
Query: 1072 ETHCVEESIHVKFDD-REPGNKTPEQSESNAGTTDSEDASESDQPSDSE-KHSEVESRPE 1129
T VEESI+VKFDD E+ N +D ++ +++ SE H E ++P+
Sbjct: 307 RTQVVEESINVKFDD*SIEATYRLEKDFLNLDLSDQDEEVPTNEGQPSETSHEEPSTQPQ 128
>BP075943
Length = 547
Score = 79.0 bits (193), Expect = 6e-15
Identities = 47/157 (29%), Positives = 78/157 (48%), Gaps = 1/157 (0%)
Frame = -3
Query: 1401 IYVDDIIFGSTNASLCKEFSKLMQDEFEMSMMGELKFFLGIQINQSKEGVYVHQTKYTKE 1460
+YVDD++ + + + D+F + +GE K+FLG++I +S G+ ++Q KY +
Sbjct: 515 LYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEIARSTSGIVLNQRKYALQ 336
Query: 1461 LLKKFKLEDCKVMNTPMHPTCTLSKEDTGT-VVDQKLYRGMIGSLLYLTASRPDILFSVC 1519
L+ + +TGT + D YR ++G LLYL +RPDI F+V
Sbjct: 335 LISDSGHFGFQPRFYSHGTNSQTLGTNTGTPLTDIGSYRRIVGRLLYLNTTRPDITFAVN 156
Query: 1520 LCARFQSDPRESHLTAVKRIFRYLKGTTNLGLLYRKS 1556
++F S P + H + +YL G+ GL Y S
Sbjct: 155 QLSQFLSAPTDIHEQQLTGFLKYL*GSPGSGLFYPAS 45
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 49.7 bits (117), Expect(2) = 1e-12
Identities = 22/51 (43%), Positives = 36/51 (70%)
Frame = +3
Query: 1591 ISWASKRQATIAMSTAEAEYISAASCCTQLLWMKHQLEDYQINANSIPIYC 1641
++W S+ Q +A+STAEAE+I+A C +LLWMK+ L++ +++ PI C
Sbjct: 27 VAWPSRLQKCVALSTAEAEFIAATEACHELLWMKNFLQNAWFHSH--PILC 173
Score = 42.0 bits (97), Expect(2) = 1e-12
Identities = 15/30 (50%), Positives = 23/30 (76%)
Frame = +2
Query: 1639 IYCDNTAAICLSKNPILHSRAKHIEIKHHF 1668
+YCDN AI L +N HSR+KHI++++H+
Sbjct: 173 LYCDNQGAIYLGQNSTFHSRSKHIDVRYHW 262
>BP063694
Length = 511
Score = 65.5 bits (158), Expect = 7e-11
Identities = 40/116 (34%), Positives = 58/116 (49%), Gaps = 3/116 (2%)
Frame = -2
Query: 800 VWHKRLGHANWRLISKISK---LQLVKGLPNIDYHSDALCGACQKGKIVKSTFKSKDIVS 856
+WH RLGH N+ +I ++ K L + LP C +CQ K + F +
Sbjct: 360 LWHSRLGHVNFDIIKQLHKHGCLDVSSILPK-----PICCTSCQMAKSKRLVFHDNN-KR 199
Query: 857 TSRPLELLHIDLFGPVNTASLYGSKYGLVIVDDYSRWTWVKFIKSKDYACEVFSSF 912
S L+L+H DL GP AS+ G Y ++ VDD+SR+TW +K K +V F
Sbjct: 198 ASAVLDLIHCDLRGPSPVASIDGFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLLRF 31
>AV766665
Length = 601
Score = 62.8 bits (151), Expect = 4e-10
Identities = 34/76 (44%), Positives = 44/76 (57%)
Frame = +3
Query: 1534 TAVKRIFRYLKGTTNLGLLYRKSLDYKLIGFCDADYAGDRIERKSTSGNCQFLGENLISW 1593
T RIF YLK + G L++K + GF ADY G ++R ST G FL NL++W
Sbjct: 360 TFTTRIF*YLKANSRRGPLFQKEGKSSMDGFTYADYLGSIVDRLSTMGYYMFLSGNLVTW 539
Query: 1594 ASKRQATIAMSTAEAE 1609
SK+Q IA S+ EAE
Sbjct: 540 RSKQQNIIARSSGEAE 587
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 60.1 bits (144), Expect = 3e-09
Identities = 35/145 (24%), Positives = 73/145 (50%), Gaps = 9/145 (6%)
Frame = +2
Query: 1371 NFLIKNDFERGQVDTTLFGKTLK-KDILIVQIYVDDIIFGSTNASLCKEFSKLMQDEFEM 1429
+F++ + R D + K D +I+ +YVDD++ N +E + EF+M
Sbjct: 8 SFIMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREFDM 187
Query: 1430 SMMGELKFFLGIQINQSKEG--VYVHQTKYTKELLKKFKLEDCKVMNTPMHPTCTLSKED 1487
+G LG+QI++ ++ +++ Q Y +++L++F ++DC ++TP+ LS
Sbjct: 188 KDLGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSSSM 367
Query: 1488 TGTVVDQKL------YRGMIGSLLY 1506
+ +++ Y +GSL+Y
Sbjct: 368 IPSSEAERMEMSRVPYASAVGSLMY 442
>AV427921
Length = 284
Score = 58.9 bits (141), Expect = 6e-09
Identities = 32/79 (40%), Positives = 43/79 (53%)
Frame = +1
Query: 958 TPQQNGVVERKNRTLQEMARTMIHENNLAKHFWAEAVNTSCYIQNRIYIRPMLEKTAYEL 1017
TPQQNGV ERKNRT+ M R+++ + L K F EAV S +I NR + E
Sbjct: 22 TPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQNMMPEEA 201
Query: 1018 FKGRRPNISYFHQFGCTCY 1036
+ GR+P + +F C Y
Sbjct: 202 WSGRQPAVDHFRISRCLAY 258
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 58.2 bits (139), Expect = 1e-08
Identities = 44/148 (29%), Positives = 68/148 (45%), Gaps = 6/148 (4%)
Frame = -1
Query: 1454 QTKYTKELLKKFKLEDCKVMNTPM-HPTCTLSKEDTGTVVDQKLYRGMIGSLLYLTASRP 1512
Q Y ++LKKFK+ + K ++T + + + G VD Y+ +IGS+ YL R
Sbjct: 739 QKIYVDDILKKFKMTNSKYISTTIGGKEIEAGRRNGGKRVDSTYYKSLIGSVRYLNTVRS 560
Query: 1513 DILFSVCLCARFQSDPRESHLTAVKRIFRYLKGTTNLGLLYR-----KSLDYKLIGFCDA 1567
DI+ V L +RF +P + H +R RY+KGT G+ SLD +++
Sbjct: 559 DIVCGVGLRSRFM-EP*DCH*QGAQRSLRYIKGTLKDGIFMLVITM*NSLDTQIV----- 398
Query: 1568 DYAGDRIERKSTSGNCQFLGENLISWAS 1595
+ G LG ISW+S
Sbjct: 397 ----------TRPGYAFHLGRGAISWSS 344
>AU088980
Length = 360
Score = 55.5 bits (132), Expect = 7e-08
Identities = 29/112 (25%), Positives = 52/112 (45%)
Frame = +2
Query: 962 NGVVERKNRTLQEMARTMIHENNLAKHFWAEAVNTSCYIQNRIYIRPMLEKTAYELFKGR 1021
N +VERK+R + + R ++ + L K+ W AV + Y+ N + + + Y+
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAYLINXLPSPLLKGQCPYQFLNND 181
Query: 1022 RPNISYFHQFGCTCYILNTKDYLKKFDAKAQRGIFLGYSERSKAYRVYNSET 1073
P + FG CY +K D +A++ +FLG+ + Y V + T
Sbjct: 182 SPTLLDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKTGTXGYIVMDLPT 337
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 54.3 bits (129), Expect = 2e-07
Identities = 22/58 (37%), Positives = 37/58 (62%), Gaps = 1/58 (1%)
Frame = +1
Query: 1622 WMKHQLEDYQINANSIP-IYCDNTAAICLSKNPILHSRAKHIEIKHHFIRDYVQKGIL 1678
W+ + L+D ++ +YCDN +A ++ NP+ H R KHIEI H +R+ +QKG++
Sbjct: 4 WLTYLLQDLKVPFEQPALVYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGLI 177
>BP052756
Length = 488
Score = 52.8 bits (125), Expect = 5e-07
Identities = 40/117 (34%), Positives = 63/117 (53%), Gaps = 1/117 (0%)
Frame = +1
Query: 345 FSSSKFRKQIKKSLMATWEDLDSESGSDKEEAEDDTKAAMGLVATVSSEA-VSEAESDSE 403
F + RK K + T++ DS D+ E+E+D +A L+AT S A SE +SDSE
Sbjct: 142 FQFKRPRKVFTKKALITFDATDS----DEAESEED-EAVEALMATTSRGAEASEDDSDSE 306
Query: 404 DENEVYSKIPRQELVDSLKELLSLFEHRTNELTDLKEKYVDLMKQQKSTLLELKASE 460
+++EV+S EL +L E++ + N+ +LK+KY +K S + E SE
Sbjct: 307 NDDEVFSDFSLSELRTALSEIMKKHDILLNKHKELKKKYA--VKTSSSEVHEKSVSE 471
>BP057233
Length = 473
Score = 49.7 bits (117), Expect = 4e-06
Identities = 20/35 (57%), Positives = 28/35 (79%)
Frame = -2
Query: 1639 IYCDNTAAICLSKNPILHSRAKHIEIKHHFIRDYV 1673
++CDN +A L+ NP+LH+R+KHIEI H+IRD V
Sbjct: 445 LWCDNLSAKALASNPVLHARSKHIEIDVHYIRDQV 341
>AV424544
Length = 276
Score = 48.9 bits (115), Expect = 7e-06
Identities = 24/90 (26%), Positives = 49/90 (53%)
Frame = +3
Query: 1368 RLSNFLIKNDFERGQVDTTLFGKTLKKDILIVQIYVDDIIFGSTNASLCKEFSKLMQDEF 1427
+LS++L + + D +LF K ++ +YVDD+I + + + + +F
Sbjct: 6 KLSSYLHILGYIQSAHDHSLFTKFRDASFTVILVYVDDLILAGNDLNEIQCVKNKLDIQF 185
Query: 1428 EMSMMGELKFFLGIQINQSKEGVYVHQTKY 1457
+ +G LK+FLG+++ +S G+++ Q KY
Sbjct: 186 RIKDLGTLKYFLGLEVARSSCGLFLSQRKY 275
>BP033602
Length = 533
Score = 47.8 bits (112), Expect = 1e-05
Identities = 28/99 (28%), Positives = 53/99 (53%)
Frame = +3
Query: 793 SMNDKKWVWHKRLGHANWRLISKISKLQLVKGLPNIDYHSDALCGACQKGKIVKSTFKSK 852
S+ D+ +WH RLGH N++ + + L K N++ S C +C K ++ + S+
Sbjct: 255 SVRDQIMLWHNRLGHPNFQYLRHLFP-DLFK---NVNC-SSLECESCVLAKNQRAPYYSQ 419
Query: 853 DIVSTSRPLELLHIDLFGPVNTASLYGSKYGLVIVDDYS 891
SRP L+H D++GP + +G ++ + +DD++
Sbjct: 420 PY-HASRPFYLIHSDVWGPSKITTQFGKRWFVTFIDDHT 533
>BP043850
Length = 515
Score = 43.9 bits (102), Expect = 2e-04
Identities = 16/62 (25%), Positives = 38/62 (60%), Gaps = 1/62 (1%)
Frame = -1
Query: 1618 TQLLWMKHQLEDYQ-INANSIPIYCDNTAAICLSKNPILHSRAKHIEIKHHFIRDYVQKG 1676
++++W++ L + + + P++ DNT+AI ++ NP+ H +HIE+ H +R+ +
Sbjct: 515 SEIIWLRGLLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYDRR 336
Query: 1677 IL 1678
++
Sbjct: 335 VI 330
>TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%)
Length = 550
Score = 43.5 bits (101), Expect = 3e-04
Identities = 23/61 (37%), Positives = 36/61 (58%), Gaps = 1/61 (1%)
Frame = +3
Query: 1619 QLLWMKHQLEDYQI-NANSIPIYCDNTAAICLSKNPILHSRAKHIEIKHHFIRDYVQKGI 1677
+ LW+ + L D +I + + IYCDN +A+ L+ N + H R ++IEI H + V GI
Sbjct: 21 EALWLTYALADLRIASLLLVVIYCDNRSALHLAANSVFHKRTENIEIDCHIV*VKVLFGI 200
Query: 1678 L 1678
L
Sbjct: 201 L 203
>AV766088
Length = 501
Score = 43.1 bits (100), Expect = 4e-04
Identities = 31/107 (28%), Positives = 57/107 (52%), Gaps = 4/107 (3%)
Frame = -3
Query: 800 VWHKRLGHANWRLISKISKLQLVKGLPNIDY---HSDALCGACQKGKIVKSTFKSKDIVS 856
+WH RLGH + S L+ ++ I Y + +C +C GK V+ F S + V
Sbjct: 307 LWHSRLGHPS------SSALRYLRSNKFISYELLNYSPVCESCVFGKHVRLPFVSSNNV- 149
Query: 857 TSRPLELLHIDLF-GPVNTASLYGSKYGLVIVDDYSRWTWVKFIKSK 902
T P ++LH DL+ PV +++ G ++ ++ +DD++ + W + +K
Sbjct: 148 TVMPFDILHSDLWTSPVLSSA--GHRFYVLFLDDFTDFLWTFPLSNK 14
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.314 0.131 0.374
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,605,659
Number of Sequences: 28460
Number of extensions: 347492
Number of successful extensions: 1557
Number of sequences better than 10.0: 94
Number of HSP's better than 10.0 without gapping: 1515
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1543
length of query: 1716
length of database: 4,897,600
effective HSP length: 103
effective length of query: 1613
effective length of database: 1,966,220
effective search space: 3171512860
effective search space used: 3171512860
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 62 (28.5 bits)
Medicago: description of AC149496.5