BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149494.2 - phase: 0
(268 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9588 138 1e-33
TC12466 similar to UP|Q8RX71 (Q8RX71) At3g51780/ORF3, partial (18%) 130 2e-31
TC16345 weakly similar to UP|Q8RX71 (Q8RX71) At3g51780/ORF3, par... 70 5e-13
TC8720 weakly similar to UP|Q8RX71 (Q8RX71) At3g51780/ORF3, part... 68 1e-12
AV772355 57 4e-09
AV426390 31 0.19
AV415704 30 0.33
AV419178 30 0.56
TC19839 weakly similar to UP|Q8VYI1 (Q8VYI1) At1g69640/F24J1.22,... 30 0.56
TC16761 similar to GB|AAL32703.1|17065098|AY062625 nucleotide su... 29 0.95
TC15402 UP|CAE45587 (CAE45587) Vacuolar proton-ATPase subunit-li... 28 2.1
TC16426 weakly similar to UP|Q9LIP0 (Q9LIP0) Genomic DNA, chromo... 27 2.8
TC8069 similar to UP|MSRA_LACSA (Q9SEC2) Peptide methionine sulf... 27 2.8
TC18806 similar to UP|Q8S564 (Q8S564) 4-coumarate:coenzyme A lig... 27 3.6
BP043924 27 3.6
BP062620 27 3.6
TC13594 homologue to GB|AAP47511.1|32331967|AY164937 RTNLB18w {M... 27 3.6
AV420704 27 4.7
AV413234 27 4.7
TC10240 27 4.7
>TC9588
Length = 644
Score = 138 bits (347), Expect = 1e-33
Identities = 83/138 (60%), Positives = 96/138 (69%), Gaps = 12/138 (8%)
Frame = +1
Query: 143 RVQKYVETLDMLKIKNS----NGGHVPKKKPQQK----VKLPPIDEQLEGMSIGNHKLQP 194
RVQKYVETLD+LK+KNS NGGH + KPQQK +L I EQ + +S G+H+LQ
Sbjct: 1 RVQKYVETLDILKVKNSMAKTNGGHA-QMKPQQKHLNGKRLATIQEQPQSVSNGHHRLQQ 177
Query: 195 SLEQQSQRNSNGNSQVFQQLQHKPSTNSTSEVVVTTKWETFDSLPPLIPVTSASSSSSS- 253
++EQQ QR+SNGNS SEVVVTTKWETFDSLPPLIPVTS SSSS+S
Sbjct: 178 NVEQQ-QRDSNGNSN--------------SEVVVTTKWETFDSLPPLIPVTSTSSSSTSS 312
Query: 254 ---TNNSVHPKFKWEHFE 268
TNNSVHPKF WE F+
Sbjct: 313 SVMTNNSVHPKFNWEFFD 366
>TC12466 similar to UP|Q8RX71 (Q8RX71) At3g51780/ORF3, partial (18%)
Length = 886
Score = 130 bits (328), Expect = 2e-31
Identities = 65/145 (44%), Positives = 104/145 (70%)
Frame = +3
Query: 16 TGLHHQDQKIFYKNKERDSKAFLDIVGVKDKSKLVVMEDPIAQEKRYLEMRKNIKMERAA 75
TGL ++Q++F++ +E++++ + GVKDKS+L+++ED ++E++ E+RK+ KM +A
Sbjct: 102 TGLKPEEQRLFFRGEEKENEEHFPVEGVKDKSELLLLEDGASKERKLEELRKHNKMLKAT 281
Query: 76 KSISEISLEVDRLAGQVSALETIISKGGKVVETDVLSLIEKLMNQLLKLDGIVADGDVKL 135
++I+ + EVD L+ +VSALE + G KV + + + E LM QLL LDGI A+G+ KL
Sbjct: 282 EAIAGVRAEVDMLSERVSALEVAVDGGTKVSDKEFVVSTELLMRQLLTLDGIEAEGEAKL 461
Query: 136 QRKMQVKRVQKYVETLDMLKIKNSN 160
QRK +V+RVQ V+TLD LK +NSN
Sbjct: 462 QRKAEVRRVQNLVDTLDSLKARNSN 536
Score = 25.8 bits (55), Expect = 8.1
Identities = 15/25 (60%), Positives = 17/25 (68%), Gaps = 1/25 (4%)
Frame = +1
Query: 227 VVTTKWETFDS-LPPLIPVTSASSS 250
+VTT WETFDS + L TS SSS
Sbjct: 565 LVTTPWETFDSGMGSLNAPTSNSSS 639
>TC16345 weakly similar to UP|Q8RX71 (Q8RX71) At3g51780/ORF3, partial (25%)
Length = 567
Score = 69.7 bits (169), Expect = 5e-13
Identities = 33/81 (40%), Positives = 57/81 (69%)
Frame = +2
Query: 6 GELKKMLTGPTGLHHQDQKIFYKNKERDSKAFLDIVGVKDKSKLVVMEDPIAQEKRYLEM 65
G++KK+L TGL ++Q++F++ E+D K L + GVKDKSKL+++E ++E++ E
Sbjct: 245 GDIKKLLVHKTGLEPEEQRLFFRGIEKDDKENLHLGGVKDKSKLLLLEGNTSKERKLEET 424
Query: 66 RKNIKMERAAKSISEISLEVD 86
RK +M +A+++IS + EVD
Sbjct: 425 RKLNEMIKASEAISRVKAEVD 487
>TC8720 weakly similar to UP|Q8RX71 (Q8RX71) At3g51780/ORF3, partial (24%)
Length = 667
Score = 68.2 bits (165), Expect = 1e-12
Identities = 54/167 (32%), Positives = 73/167 (43%)
Frame = +3
Query: 102 GGKVVETDVLSLIEKLMNQLLKLDGIVADGDVKLQRKMQVKRVQKYVETLDMLKIKNSNG 161
G + E + L L E LM+QLLKLDGI A+G+ +LQRK +V+RVQ V+ +D LK +NSN
Sbjct: 3 GNEASEKEFLELSELLMSQLLKLDGIEAEGEARLQRKAEVRRVQNIVDAIDSLKARNSN- 179
Query: 162 GHVPKKKPQQKVKLPPIDEQLEGMSIGNHKLQPSLEQQSQRNSNGNSQVFQQLQHKPSTN 221
P ID+ GN+
Sbjct: 180 --------------PFIDK-------------------------GNA------------- 203
Query: 222 STSEVVVTTKWETFDSLPPLIPVTSASSSSSSTNNSVHPKFKWEHFE 268
V VTT+WE FDS T +S +S+T +S WE F+
Sbjct: 204 ----VTVTTQWEAFDS------GTESSKDTSTTLSSTKITEDWERFD 314
>AV772355
Length = 115
Score = 56.6 bits (135), Expect = 4e-09
Identities = 27/34 (79%), Positives = 30/34 (87%)
Frame = +1
Query: 107 ETDVLSLIEKLMNQLLKLDGIVADGDVKLQRKMQ 140
ETD+LSL+E LMNQLL+LDGI DGDVK QRKMQ
Sbjct: 13 ETDLLSLVELLMNQLLRLDGIAGDGDVKWQRKMQ 114
>AV426390
Length = 399
Score = 31.2 bits (69), Expect = 0.19
Identities = 15/43 (34%), Positives = 21/43 (47%)
Frame = +3
Query: 177 PIDEQLEGMSIGNHKLQPSLEQQSQRNSNGNSQVFQQLQHKPS 219
P Q GMS+ N + QP +QQ Q +S+ + H PS
Sbjct: 30 PDHHQFPGMSLSNQQQQPQQQQQQQHHSHPDCASLIANSHSPS 158
>AV415704
Length = 355
Score = 30.4 bits (67), Expect = 0.33
Identities = 19/43 (44%), Positives = 26/43 (60%)
Frame = +1
Query: 225 EVVVTTKWETFDSLPPLIPVTSASSSSSSTNNSVHPKFKWEHF 267
E +V T+W L L P +S+SSSSS++ S +PK KW F
Sbjct: 124 ESLVRTRWP----LLALAPSSSSSSSSSTSATSPNPK-KWRKF 237
>AV419178
Length = 401
Score = 29.6 bits (65), Expect = 0.56
Identities = 13/39 (33%), Positives = 22/39 (56%)
Frame = -3
Query: 211 FQQLQHKPSTNSTSEVVVTTKWETFDSLPPLIPVTSASS 249
++ H PS+N +S V + +T + PPL+P + SS
Sbjct: 339 YRSFSHSPSSNFSSSVSKISGMKTISTKPPLLPSSIFSS 223
>TC19839 weakly similar to UP|Q8VYI1 (Q8VYI1) At1g69640/F24J1.22, partial
(13%)
Length = 412
Score = 29.6 bits (65), Expect = 0.56
Identities = 14/28 (50%), Positives = 17/28 (60%)
Frame = -1
Query: 211 FQQLQHKPSTNSTSEVVVTTKWETFDSL 238
F+Q Q P TNS S+ +TTK FD L
Sbjct: 229 FKQKQPNPETNSASQSCITTKDLNFDKL 146
>TC16761 similar to GB|AAL32703.1|17065098|AY062625 nucleotide sugar
epimerase-like protein {Arabidopsis thaliana;}, partial
(40%)
Length = 794
Score = 28.9 bits (63), Expect = 0.95
Identities = 21/56 (37%), Positives = 31/56 (54%), Gaps = 5/56 (8%)
Frame = +1
Query: 210 VFQQLQHKP-STNSTSEVVVTTKWETFDSLPP----LIPVTSASSSSSSTNNSVHP 260
+ Q Q KP S N T+ V + ++F +LPP P +S SSSSSS+ ++ P
Sbjct: 175 LLHQTQAKP*SWNVTTATSVKSTAQSFSTLPPNSSSAPPSSSHSSSSSSSPSTTLP 342
>TC15402 UP|CAE45587 (CAE45587) Vacuolar proton-ATPase subunit-like protein,
complete
Length = 2423
Score = 27.7 bits (60), Expect = 2.1
Identities = 21/81 (25%), Positives = 33/81 (39%), Gaps = 8/81 (9%)
Frame = +1
Query: 126 GIVADGDVKLQRKMQVKRVQKYVETLDMLKIKNSNGGHVPKKKPQQ--------KVKLPP 177
G++ DV + ++KR + L K + G PK Q +VKL
Sbjct: 133 GLLQFKDVSNSKPFKIKRCGEMARKLRFFKEQMLKAGVSPKLSTTQVDVNIDNLEVKLSE 312
Query: 178 IDEQLEGMSIGNHKLQPSLEQ 198
I+ +L M+ KLQ S +
Sbjct: 313 IESELTEMNANGEKLQRSYNE 375
>TC16426 weakly similar to UP|Q9LIP0 (Q9LIP0) Genomic DNA, chromosome 3, BAC
clone:F20C19, partial (82%)
Length = 518
Score = 27.3 bits (59), Expect = 2.8
Identities = 25/113 (22%), Positives = 56/113 (49%), Gaps = 8/113 (7%)
Frame = +2
Query: 83 LEVDRLAGQVSALETIISKGGKVVETDVLSLIEKLMNQLLKLDGI---VADGDVK----- 134
LE++ +A ++S+L G T+ L+ + QL + GI V +G+++
Sbjct: 143 LEMNSIARRLSSLF-----GKSACATEPLNSGRHQLQQLQQCRGIRVRVFNGNLESALAL 307
Query: 135 LQRKMQVKRVQKYVETLDMLKIKNSNGGHVPKKKPQQKVKLPPIDEQLEGMSI 187
+QRKM +++ ++ IKNS + +K +++++ + +L+ + I
Sbjct: 308 MQRKMTSSGIERMIKKEQRFHIKNSEKRVLARKNLERRLRSEDLARKLKAIMI 466
>TC8069 similar to UP|MSRA_LACSA (Q9SEC2) Peptide methionine sulfoxide
reductase (Protein-methionine-S-oxide reductase)
(Peptide Met(O) reductase) , partial (71%)
Length = 1267
Score = 27.3 bits (59), Expect = 2.8
Identities = 14/33 (42%), Positives = 19/33 (57%), Gaps = 6/33 (18%)
Frame = +1
Query: 238 LPPLIPVTSASSSSSST------NNSVHPKFKW 264
LPP +P+ + S SSSST N+S+ P W
Sbjct: 19 LPPRLPLFAVSESSSSTTIEAFSNSSLTPTLTW 117
>TC18806 similar to UP|Q8S564 (Q8S564) 4-coumarate:coenzyme A ligase ,
partial (26%)
Length = 529
Score = 26.9 bits (58), Expect = 3.6
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = -1
Query: 234 TFDSLPPLIPVTSASSSSSSTNNSVHPKFKWEHF 267
TF +L PL+ ++ S + S++P+F HF
Sbjct: 466 TFHNLVPLMQISPGVVSLEGKHRSLYPRFSSRHF 365
>BP043924
Length = 546
Score = 26.9 bits (58), Expect = 3.6
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +1
Query: 200 SQRNSNGNSQVFQQLQHKPSTNSTSEVVVTTKW 232
S RNS+ S + +L KP+ S S V +T+W
Sbjct: 100 SPRNSSSPSSISSKLILKPANRSRSHVNGSTRW 198
>BP062620
Length = 555
Score = 26.9 bits (58), Expect = 3.6
Identities = 23/79 (29%), Positives = 37/79 (46%), Gaps = 10/79 (12%)
Frame = +2
Query: 151 LDMLKIKNSNGGHVPKKKPQQKVKLPPIDEQLE--GMSIGNHKLQPSLEQQSQRNSNGNS 208
++ +K K G P + P PP D + G+++G H L + + QRN N +S
Sbjct: 254 VEWMKNKPDLKGGNPDRSP------PPADLKHSD*GINLGKHGLHHQIIRHHQRNXNLSS 415
Query: 209 ------QVFQQ--LQHKPS 219
Q+FQ+ HKP+
Sbjct: 416 *XSPYTQIFQKNTNNHKPT 472
>TC13594 homologue to GB|AAP47511.1|32331967|AY164937 RTNLB18w {Medicago
truncatula;} , partial (41%)
Length = 556
Score = 26.9 bits (58), Expect = 3.6
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = -3
Query: 201 QRNSNGNSQVFQQLQHKPSTNSTSEVVVTTKW 232
Q++SN + F+ +HK + +S + TTKW
Sbjct: 554 QKSSNSVNSQFRSAEHKTAAAVSSSNI*TTKW 459
>AV420704
Length = 245
Score = 26.6 bits (57), Expect = 4.7
Identities = 18/45 (40%), Positives = 24/45 (53%)
Frame = +3
Query: 218 PSTNSTSEVVVTTKWETFDSLPPLIPVTSASSSSSSTNNSVHPKF 262
PST+S V+T W S P L TS++SSS S+ P+F
Sbjct: 108 PSTSS-----VSTWWRASTSSPTLSASTSSTSSSVSSPLRSIPRF 227
>AV413234
Length = 378
Score = 26.6 bits (57), Expect = 4.7
Identities = 18/54 (33%), Positives = 26/54 (47%)
Frame = -1
Query: 214 LQHKPSTNSTSEVVVTTKWETFDSLPPLIPVTSASSSSSSTNNSVHPKFKWEHF 267
L H PS +S+S ++ F +LPPL T +S+ +ST F HF
Sbjct: 219 LLHHPSLSSSSVLIN*AFTFFFMALPPLQVETLEASTPTSTTTGFFKIFFMFHF 58
>TC10240
Length = 575
Score = 26.6 bits (57), Expect = 4.7
Identities = 18/51 (35%), Positives = 22/51 (42%)
Frame = -3
Query: 194 PSLEQQSQRNSNGNSQVFQQLQHKPSTNSTSEVVVTTKWETFDSLPPLIPV 244
PSL Q + + VF HKPS +VV W S PPL P+
Sbjct: 297 PSLSHQWRMVARSCGSVFLGHLHKPSPEHHWKVVDLPLWLC*CSNPPLSPL 145
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.311 0.129 0.350
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,755,201
Number of Sequences: 28460
Number of extensions: 45890
Number of successful extensions: 477
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 430
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 458
length of query: 268
length of database: 4,897,600
effective HSP length: 89
effective length of query: 179
effective length of database: 2,364,660
effective search space: 423274140
effective search space used: 423274140
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 54 (25.4 bits)
Medicago: description of AC149494.2