BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149491.7 - phase: 0
(114 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AI967770 115 1e-27
AV771869 30 0.074
BP040333 28 0.22
TC8502 homologue to UP|Q84P31 (Q84P31) Aldehyde dehydrogenase fa... 28 0.28
BP035958 26 0.82
TC8037 similar to UP|AAR14273 (AAR14273) Predicted protein, part... 26 0.82
TC13462 26 1.1
TC16585 25 1.4
AU089616 25 1.4
TC18472 25 2.4
BP070747 25 2.4
AU251465 24 3.1
BP055222 24 3.1
TC11202 24 3.1
TC19282 similar to UP|Q84XU4 (Q84XU4) Protein disulphide isomera... 24 4.1
TC11768 24 4.1
AV406949 24 4.1
TC15740 weakly similar to GB|AAM19935.1|20453393|AY097419 AT5g58... 23 5.3
TC12715 similar to UP|Q9FFQ1 (Q9FFQ1) RNA helicase, partial (20%) 23 5.3
TC16740 similar to UP|Q9SIJ8 (Q9SIJ8) Expressed protein (RD2 pro... 23 5.3
>AI967770
Length = 269
Score = 115 bits (288), Expect = 1e-27
Identities = 50/56 (89%), Positives = 53/56 (94%)
Frame = +2
Query: 1 MSTMKFCRECNNILYPKEDREQKILLYACRNCDHQEAADNFCVYRNEIHHSVAERT 56
MSTMKFCRECNNILYPKEDR+QK+LLYACRNCDHQE AD+ CVYRNEIHHSV ERT
Sbjct: 101 MSTMKFCRECNNILYPKEDRDQKVLLYACRNCDHQEVADSCCVYRNEIHHSVGERT 268
>AV771869
Length = 429
Score = 29.6 bits (65), Expect = 0.074
Identities = 12/36 (33%), Positives = 20/36 (55%), Gaps = 1/36 (2%)
Frame = -1
Query: 80 NHGEAVFFQATARG-EEGMTLFFVCCNPNCGHRWRD 114
+H +A + + R +E T+F+ C N C H WR+
Sbjct: 306 SHDKAAYPEMQIRSADEPATIFYWCLNEKCRHPWRE 199
>BP040333
Length = 480
Score = 28.1 bits (61), Expect = 0.22
Identities = 17/51 (33%), Positives = 27/51 (52%)
Frame = -3
Query: 15 YPKEDREQKILLYACRNCDHQEAADNFCVYRNEIHHSVAERTQVLQDVAAD 65
Y E+KI L CRNCD++ +DN + E H+ E+ + +D + D
Sbjct: 415 YSDLTHEEKISLLFCRNCDYE--SDNEPDHTQE-PHAEKEKGYLSEDASED 272
>TC8502 homologue to UP|Q84P31 (Q84P31) Aldehyde dehydrogenase family 7
member A1 , partial (65%)
Length = 1415
Score = 27.7 bits (60), Expect = 0.28
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +3
Query: 92 RGEEGMTLFFVCCNPNCGHRW 112
R + G TL+FVCC CG+ W
Sbjct: 357 RHQTGCTLYFVCC---CGYCW 410
>BP035958
Length = 574
Score = 26.2 bits (56), Expect = 0.82
Identities = 13/29 (44%), Positives = 14/29 (47%)
Frame = -1
Query: 83 EAVFFQATARGEEGMTLFFVCCNPNCGHR 111
EA AT E M + VC NPNC R
Sbjct: 199 EAEAEAATETSETAMETWPVCVNPNCSKR 113
>TC8037 similar to UP|AAR14273 (AAR14273) Predicted protein, partial (5%)
Length = 895
Score = 26.2 bits (56), Expect = 0.82
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = +1
Query: 91 ARGEEGMTLFFVCCNPNC 108
AR EEG+ F+CCN C
Sbjct: 637 ARREEGIA*LFICCN*AC 690
>TC13462
Length = 625
Score = 25.8 bits (55), Expect = 1.1
Identities = 13/47 (27%), Positives = 24/47 (50%), Gaps = 3/47 (6%)
Frame = +3
Query: 6 FCRECNNILYPKEDREQKILLYACRNCD---HQEAADNFCVYRNEIH 49
FCR CN + +R +K+L +C++C H+ ++ R+ H
Sbjct: 486 FCRMCNRVENEGSERAKKML--SCKSCSKKYHRTCLKSWAQNRDLFH 620
>TC16585
Length = 426
Score = 25.4 bits (54), Expect = 1.4
Identities = 16/48 (33%), Positives = 21/48 (43%)
Frame = -2
Query: 54 ERTQVLQDVAADPTLPRTKAVRCVQCNHGEAVFFQATARGEEGMTLFF 101
E T+ +AD PRTK +HG + A EE MT+ F
Sbjct: 161 ESTRATLSTSADGWSPRTKGAAASSPSHGVSAPILAGTESEE-MTILF 21
>AU089616
Length = 375
Score = 25.4 bits (54), Expect = 1.4
Identities = 8/13 (61%), Positives = 9/13 (68%)
Frame = -3
Query: 102 VCCNPNCGHRWRD 114
+CC PNC H RD
Sbjct: 283 ICCLPNCNHTPRD 245
>TC18472
Length = 642
Score = 24.6 bits (52), Expect = 2.4
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = -1
Query: 7 CRECNNILYPKEDREQKIL 25
CR C+NI+ E+ QK+L
Sbjct: 639 CRACDNIIAGSENLSQKVL 583
>BP070747
Length = 425
Score = 24.6 bits (52), Expect = 2.4
Identities = 10/45 (22%), Positives = 21/45 (46%)
Frame = +2
Query: 34 HQEAADNFCVYRNEIHHSVAERTQVLQDVAADPTLPRTKAVRCVQ 78
HQ +C++ + HH +RT+ + + P P +R ++
Sbjct: 287 HQILLHKYCIHPLDFHHRSFQRTRYSKFLTLPPPYPYLLHLRVLE 421
>AU251465
Length = 363
Score = 24.3 bits (51), Expect = 3.1
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = +2
Query: 103 CCNPNCGHRW 112
CCNP C RW
Sbjct: 317 CCNPCCNGRW 346
>BP055222
Length = 536
Score = 24.3 bits (51), Expect = 3.1
Identities = 7/13 (53%), Positives = 10/13 (76%)
Frame = -2
Query: 100 FFVCCNPNCGHRW 112
FF C +P+ G+RW
Sbjct: 193 FFTCSDPSVGNRW 155
>TC11202
Length = 537
Score = 24.3 bits (51), Expect = 3.1
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = -1
Query: 89 ATARGEEGMTLFFVCC 104
A+A G TLFF+CC
Sbjct: 183 ASANNIHGSTLFFLCC 136
>TC19282 similar to UP|Q84XU4 (Q84XU4) Protein disulphide isomerase
(Fragment), partial (17%)
Length = 520
Score = 23.9 bits (50), Expect = 4.1
Identities = 14/42 (33%), Positives = 19/42 (44%), Gaps = 6/42 (14%)
Frame = -1
Query: 76 CVQCNHGEAVFFQATARGEEGMTLF------FVCCNPNCGHR 111
C+ C H ARGE+ + LF F+C N N +R
Sbjct: 439 CLHCPHLPPFLCLLLARGEKKLGLFSLSHYVFMCLNHNSINR 314
>TC11768
Length = 674
Score = 23.9 bits (50), Expect = 4.1
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = -1
Query: 41 FCVYRNEIHHSVAERTQVLQDVAADPTLPR 70
F Y NEIH +++T Q+ PT PR
Sbjct: 473 FDTYNNEIHPRRSKQTN*KQNNQPTPTPPR 384
>AV406949
Length = 443
Score = 23.9 bits (50), Expect = 4.1
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +1
Query: 57 QVLQDVAADPTLPRTKAVRCVQCNHGE 83
+VLQD+ ADP R + +R V ++ E
Sbjct: 121 RVLQDLGADPNNIRAQVIRMVGESNNE 201
>TC15740 weakly similar to GB|AAM19935.1|20453393|AY097419 AT5g58640/mzn1_90
{Arabidopsis thaliana;}, partial (65%)
Length = 575
Score = 23.5 bits (49), Expect = 5.3
Identities = 9/26 (34%), Positives = 16/26 (60%)
Frame = +3
Query: 10 CNNILYPKEDREQKILLYACRNCDHQ 35
CNN + +E+R Q ++ ++C HQ
Sbjct: 219 CNNSVPLRENRHQYWRCWSWQHCQHQ 296
>TC12715 similar to UP|Q9FFQ1 (Q9FFQ1) RNA helicase, partial (20%)
Length = 552
Score = 23.5 bits (49), Expect = 5.3
Identities = 7/23 (30%), Positives = 13/23 (56%)
Frame = -3
Query: 27 YACRNCDHQEAADNFCVYRNEIH 49
++C NC +Q D +Y + +H
Sbjct: 175 FSCWNCSNQAMPDMLLLYSSSLH 107
>TC16740 similar to UP|Q9SIJ8 (Q9SIJ8) Expressed protein (RD2 protein),
partial (90%)
Length = 765
Score = 23.5 bits (49), Expect = 5.3
Identities = 6/18 (33%), Positives = 14/18 (77%)
Frame = -3
Query: 18 EDREQKILLYACRNCDHQ 35
+++ Q++L+Y+C + HQ
Sbjct: 562 QEQSQQVLIYSCEHSTHQ 509
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.326 0.137 0.457
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,822,495
Number of Sequences: 28460
Number of extensions: 38636
Number of successful extensions: 223
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 223
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 223
length of query: 114
length of database: 4,897,600
effective HSP length: 90
effective length of query: 24
effective length of database: 2,336,200
effective search space: 56068800
effective search space used: 56068800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 47 (22.7 bits)
Medicago: description of AC149491.7