BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149490.9 - phase: 0
(1049 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP040614 84 1e-16
BP062438 84 2e-16
AV407795 82 4e-16
CB829109 81 7e-16
AV779848 78 8e-15
TC15461 similar to UP|O24147 (O24147) Kinesin-like protein, part... 69 5e-12
TC15187 similar to UP|O24147 (O24147) Kinesin-like protein, part... 59 3e-09
AV773555 52 5e-07
AV421105 50 2e-06
BP063922 45 6e-05
TC11656 42 4e-04
TC12031 homologue to UP|ATK1_ARATH (Q07970) Kinesin 1 (Kinesin-l... 38 0.009
AV411945 34 0.14
TC13297 similar to UP|SY81_ARATH (P59277) Syntaxin 81 (AtSYP81),... 32 0.68
TC16641 30 1.5
BI418008 30 2.6
BP067112 30 2.6
TC17030 similar to UP|Q8S995 (Q8S995) Glucosyltransferase-14, pa... 29 3.4
CB828195 29 3.4
CB827211 29 4.4
>BP040614
Length = 504
Score = 84.0 bits (206), Expect = 1e-16
Identities = 56/163 (34%), Positives = 89/163 (54%), Gaps = 4/163 (2%)
Frame = +1
Query: 176 AQSAEYSMKVTFLELYNEEITDLLAPEETTKFVDEKSKKPIALMEDGKGGVLVRGLEEEI 235
A S +Y + V +E+YNE++ DLL + + +D ++ + G + +
Sbjct: 55 ADSIKYEVFVQMIEIYNEQVRDLLVSNGSNRRLDIRNNSQL------NGLNVPDAYLVPV 216
Query: 236 VCTANEIYKILEKGSAKRRTAETLLNKQSSRSHSIFSITIHIKECTPEGEEMIKC----G 291
CT + +Y ++E G R T LN++SSRSHS+ +T+H++ G E++ G
Sbjct: 217 TCTQDVLY-LMEVGQKNRAVGATALNERSSRSHSV--LTVHVR-----GRELVSGSILRG 372
Query: 292 KLNLVDLAGSENISRSGAREGRAREAGEINKSLLTLGRTINAL 334
L+LVDLAGSE + +S A R +EA IN+SL LG I+AL
Sbjct: 373 CLHLVDLAGSERVEKSEAVGERLKEAQHINRSLSALGDVISAL 501
>BP062438
Length = 498
Score = 83.6 bits (205), Expect = 2e-16
Identities = 45/110 (40%), Positives = 67/110 (60%), Gaps = 1/110 (0%)
Frame = +3
Query: 126 GYNCTIFAYGQTGTGKTYTMEGGAIKKNGEFPTDAGVIPRAVKQIFDILEAQ-SAEYSMK 184
G+N T+FAYGQT +GKT+TM G D GVIPRAV+ +F I++ E+ ++
Sbjct: 222 GFNGTVFAYGQTNSGKTHTMRGS--------KADPGVIPRAVRDLFQIIQQDVDREFLLR 377
Query: 185 VTFLELYNEEITDLLAPEETTKFVDEKSKKPIALMEDGKGGVLVRGLEEE 234
++++E+YNEEI DLLAPE + + + E+ + G+ V GL EE
Sbjct: 378 MSYMEIYNEEINDLLAPEH----------RKLQIHENLERGIYVAGLREE 497
>AV407795
Length = 424
Score = 82.0 bits (201), Expect = 4e-16
Identities = 54/149 (36%), Positives = 78/149 (52%), Gaps = 1/149 (0%)
Frame = +2
Query: 187 FLELYNEEITDLLAPEETTKFVDEKSKKPIALMEDGKGGVLVRGLEEEIVCTANEIYKIL 246
F E+Y ++ DLL +K + + EDGK V + GL+E V I +++
Sbjct: 2 FFEIYGGKLFDLL-----------NDRKKLFIREDGKQQVCIVGLQEYCVSDVESIKELI 148
Query: 247 EKGSAKRRTAETLLNKQSSRSHSIFSITIHIKECTPEGEEMIKCGKLNLVDLAGSENISR 306
E+G+A R T T N++SSRSH+I + I E + GKL+ +DLAGSE +
Sbjct: 149 ERGNATRSTGTTGANEESSRSHAILQLAIKRSVDGNESKPPRPVGKLSFIDLAGSERGAD 328
Query: 307 SGAREGRAR-EAGEINKSLLTLGRTINAL 334
+ + + R E EINKSLL L I AL
Sbjct: 329 TTDNDKQTRIEGAEINKSLLALKECIRAL 415
>CB829109
Length = 514
Score = 81.3 bits (199), Expect = 7e-16
Identities = 50/160 (31%), Positives = 83/160 (51%)
Frame = +2
Query: 116 VSPIVYEVLEGYNCTIFAYGQTGTGKTYTMEGGAIKKNGEFPTDAGVIPRAVKQIFDILE 175
V PI+ + E T FAYGQTG+GKTYTM+ P V+Q+ +
Sbjct: 11 VEPIIPTIFERTKATCFAYGQTGSGKTYTMQ----------PLPLRAAEDLVRQLHRPVY 160
Query: 176 AQSAEYSMKVTFLELYNEEITDLLAPEETTKFVDEKSKKPIALMEDGKGGVLVRGLEEEI 235
Q+ ++ + +++ E+Y ++ DLL+ +K + + EDG+ V + GL+E
Sbjct: 161 -QNQKFKLWLSYFEIYGGKLFDLLS-----------DRKKLCMREDGRQQVCIVGLQEFE 304
Query: 236 VCTANEIYKILEKGSAKRRTAETLLNKQSSRSHSIFSITI 275
V + + +EKG+A R T T N++SSRSH+I + +
Sbjct: 305 VFDVQIVKEFIEKGNAARSTGSTGANEESSRSHAILQLVV 424
>AV779848
Length = 293
Score = 77.8 bits (190), Expect = 8e-15
Identities = 46/61 (75%), Positives = 48/61 (78%), Gaps = 3/61 (4%)
Frame = +3
Query: 45 EKGVNVQVLVRCRPMNE---DEMRLHTPVVISCNEGRREVAAVQSIANKQIDRTFVFDKV 101
EKGVNVQVLVRC NE D H VVISCN+GRREV AVQ+IANKQIDRTFVFDKV
Sbjct: 66 EKGVNVQVLVRCSA-NE*R*D*CYTHR-VVISCNDGRREVTAVQNIANKQIDRTFVFDKV 239
Query: 102 F 102
F
Sbjct: 240 F 242
Score = 45.4 bits (106), Expect = 5e-05
Identities = 39/95 (41%), Positives = 50/95 (52%), Gaps = 4/95 (4%)
Frame = +2
Query: 28 DLRSADSNSNSHNKYDKEKGVNV--QVLVRCRPMNEDEMRLHTPVVISCNEGRREVAAVQ 85
DLRSADSNS+SH+KYDK + ++C+ M + + LHTP +
Sbjct: 14 DLRSADSNSSSHSKYDK*ERCQCAGSGSLQCQ*M-KMRLVLHTPCCDFVQ*W*KRGYCCP 190
Query: 86 SIANKQIDR--TFVFDKVFGPNSQQKELYDQAVSP 118
+ Q DR + V P SQQKELYDQAVSP
Sbjct: 191 EY-S*QADR*NLRI*QGVLCPTSQQKELYDQAVSP 292
>TC15461 similar to UP|O24147 (O24147) Kinesin-like protein, partial (9%)
Length = 527
Score = 68.6 bits (166), Expect = 5e-12
Identities = 46/136 (33%), Positives = 66/136 (47%)
Frame = +3
Query: 328 GRTINALVEHSGHVPYRDSKLTRLLRDSLGGKTKTCIIATVSPSIHCLEETLSTLDYAHR 387
G I+AL H+PYR+ KLT L+ DSLGG KT + VSP L+ET ++L YA R
Sbjct: 3 GDVISALSSGGQHIPYRNHKLTMLMSDSLGGNAKTLMFVNVSPIESSLDETHNSLMYASR 182
Query: 388 AKNIKNKPEVNQKMMKSAMIKDLYSEIDRLKQEVYAAREKNGIYIPRDRYLNEEAEKKAM 447
++I N P N EI RLK++V Y E+A ++
Sbjct: 183 VRSIVNDPSKNVSS----------KEIMRLKKQV--------------AYWKEQAGRRGE 290
Query: 448 AEKIERMELDADSKDK 463
E +E ++ +K+K
Sbjct: 291 EEDLEEIQEVRPTKEK 338
>TC15187 similar to UP|O24147 (O24147) Kinesin-like protein, partial (8%)
Length = 611
Score = 59.3 bits (142), Expect = 3e-09
Identities = 38/106 (35%), Positives = 54/106 (50%), Gaps = 1/106 (0%)
Frame = +2
Query: 341 VPYRDSKLTRLLRDSLGGKTKTCIIATVSPSIHCLEETLSTLDYAHRAKNIKNKPEVNQK 400
+PYR+ KLT L+ DSLGG +T + VSP L+ET ++L YA R ++I N P N
Sbjct: 2 IPYRNHKLTMLMSDSLGGNAQTLMFVNVSPVESSLDETHNSLMYASRVRSIINDPSQNVA 181
Query: 401 MMKSAMIKDLYSEIDRLKQEVYAAREKNGI-YIPRDRYLNEEAEKK 445
+ A +K L + KQ E + IP +R E E +
Sbjct: 182 SKEIARLKKL---VSYWKQPAGRGLENEDLEEIPDERSSEERTESR 310
>AV773555
Length = 460
Score = 52.0 bits (123), Expect = 5e-07
Identities = 27/65 (41%), Positives = 39/65 (59%)
Frame = -1
Query: 323 SLLTLGRTINALVEHSGHVPYRDSKLTRLLRDSLGGKTKTCIIATVSPSIHCLEETLSTL 382
SL +L I AL + H+P+R SKLT LL+ +LGG +KT + +SP E+L +L
Sbjct: 460 SLSSLSDVIFALAQQDDHIPFRHSKLTYLLQPALGGDSKTVMFVNISPDPASAGESLCSL 281
Query: 383 DYAHR 387
+A R
Sbjct: 280 RFASR 266
>AV421105
Length = 415
Score = 49.7 bits (117), Expect = 2e-06
Identities = 23/53 (43%), Positives = 40/53 (75%)
Frame = +2
Query: 341 VPYRDSKLTRLLRDSLGGKTKTCIIATVSPSIHCLEETLSTLDYAHRAKNIKN 393
VPYR+SKLTR+L+DSLGG ++ ++A ++P + +E++ T+ A R+++I N
Sbjct: 5 VPYRESKLTRILQDSLGGTSRALMVACLNPGEY--QESVHTVSLAARSRHITN 157
>BP063922
Length = 537
Score = 45.1 bits (105), Expect = 6e-05
Identities = 26/64 (40%), Positives = 35/64 (54%)
Frame = +2
Query: 292 KLNLVDLAGSENISRSGAREGRAREAGEINKSLLTLGRTINALVEHSGHVPYRDSKLTRL 351
KL+LVDLAGSE R + + KSL LG +++L VPY +S LT++
Sbjct: 344 KLSLVDLAGSEGSITEDDSGERVTDLLHVMKSLSALGDVLSSLTSKKDIVPYENSALTKV 523
Query: 352 LRDS 355
L DS
Sbjct: 524 LADS 535
>TC11656
Length = 566
Score = 42.4 bits (98), Expect = 4e-04
Identities = 25/71 (35%), Positives = 39/71 (54%)
Frame = +3
Query: 938 LNSMITPCCGDLTELKGGHYNRIVEITENAGKCLLNEYMVDEPSCSTPTRRLFNLPSVSS 997
++SM LT L H I I + A K L+ +Y+VD+ + ST + + +PS +S
Sbjct: 12 IDSMTARWLEQLTSLTHKHGEDISNIHDQAEKSLVKDYLVDQNTSSTSHKIVVLVPSPAS 191
Query: 998 IEELRTPSFEE 1008
IEE+RT E+
Sbjct: 192 IEEMRTQITED 224
>TC12031 homologue to UP|ATK1_ARATH (Q07970) Kinesin 1 (Kinesin-like protein
A), partial (7%)
Length = 740
Score = 37.7 bits (86), Expect = 0.009
Identities = 19/44 (43%), Positives = 28/44 (63%)
Frame = +2
Query: 344 RDSKLTRLLRDSLGGKTKTCIIATVSPSIHCLEETLSTLDYAHR 387
R+SKLT LL+ LGG +KT + +SP + E+L +L +A R
Sbjct: 2 RNSKLTYLLQPCLGGDSKTLMFVNISPDQSSVGESLCSLRFASR 133
>AV411945
Length = 405
Score = 33.9 bits (76), Expect = 0.14
Identities = 22/102 (21%), Positives = 49/102 (47%), Gaps = 5/102 (4%)
Frame = +3
Query: 521 ISNLLKSEKELVERAIE-----LRAELENAASDVSNLFSKIERKDKIEEENRVLIQKFQS 575
++N LK E ++ ++ RAE++NA + V + ++ ++E R+ +
Sbjct: 51 LANFLKQASEDAKKLVDEERAFARAEIDNARAAVQRV------EEALKEHERMSQASGKQ 212
Query: 576 QLAQQLEALHRTVSASVMHQEQQLKDMEKDMQSFVSTKSEAT 617
L Q + + ++HQ ++ DME ++Q+ + +E T
Sbjct: 213 DLEQLKKEVQEARRIKMLHQPSKVMDMEHELQALRAQLAEKT 338
>TC13297 similar to UP|SY81_ARATH (P59277) Syntaxin 81 (AtSYP81), partial
(34%)
Length = 563
Score = 31.6 bits (70), Expect = 0.68
Identities = 26/103 (25%), Positives = 48/103 (46%), Gaps = 10/103 (9%)
Frame = +2
Query: 644 ELKSNNQLTYEDLKSEVAKHSSALEDLFKGIALEADSLLNDLQNSL----------HKQE 693
EL+ ++L E L+ + L+D + + +E +LL+ +Q + H
Sbjct: 17 ELREPDELRSEPLRVQ----QQLLDDETRVLQVELTNLLDTVQETETKMVEMSALNHLMS 184
Query: 694 ANVTAFAHQQREAHSRAVETTRSVSKITMKFFETIDRHASSLT 736
+V AHQ + +AVE T++V + + I R++SS T
Sbjct: 185 THVLQQAHQIEYLYEQAVEATKNVEFGNKELSQAIQRNSSSRT 313
>TC16641
Length = 571
Score = 30.4 bits (67), Expect = 1.5
Identities = 17/77 (22%), Positives = 37/77 (47%)
Frame = -1
Query: 136 QTGTGKTYTMEGGAIKKNGEFPTDAGVIPRAVKQIFDILEAQSAEYSMKVTFLELYNEEI 195
++GTG + GA+ G + + R V+Q+ +LE + + ++ F E ++ +
Sbjct: 247 RSGTGAGVPLNNGAVSSAGSSSSKTPLEYRLVEQVVSVLETTGSSXTHELEFEE--SKFL 74
Query: 196 TDLLAPEETTKFVDEKS 212
+ P+ T+F+ S
Sbjct: 73 VXVFIPKSLTRFLSSIS 23
>BI418008
Length = 409
Score = 29.6 bits (65), Expect = 2.6
Identities = 16/51 (31%), Positives = 28/51 (54%)
Frame = +2
Query: 421 VYAAREKNGIYIPRDRYLNEEAEKKAMAEKIERMELDADSKDKNLVELQEL 471
+Y R+ +G+Y+P +YL E EK ++R+ S+D+ + L EL
Sbjct: 200 LYLIRDTSGMYLPPFKYLTPEQEK-----DVDRLLTRLVSRDEAHIALMEL 337
>BP067112
Length = 527
Score = 29.6 bits (65), Expect = 2.6
Identities = 13/40 (32%), Positives = 23/40 (57%), Gaps = 3/40 (7%)
Frame = -3
Query: 159 DAGVIPRAVKQIFDILE---AQSAEYSMKVTFLELYNEEI 195
D G+ R +++FD+ +++Y VT ELYNE++
Sbjct: 441 DRGLYARCFEELFDLANLDTTSTSQYKFCVTVCELYNEQV 322
>TC17030 similar to UP|Q8S995 (Q8S995) Glucosyltransferase-14, partial (51%)
Length = 1098
Score = 29.3 bits (64), Expect = 3.4
Identities = 16/37 (43%), Positives = 21/37 (56%), Gaps = 1/37 (2%)
Frame = +2
Query: 15 PSQTPRSTDKPARDLRSADSNSNSH-NKYDKEKGVNV 50
P+ S +P D +S SNSNSH K D +KGV +
Sbjct: 320 PTTHHASLTQPNPDPKSESSNSNSHTTKLDYQKGVRI 430
>CB828195
Length = 551
Score = 29.3 bits (64), Expect = 3.4
Identities = 28/98 (28%), Positives = 46/98 (46%), Gaps = 6/98 (6%)
Frame = +3
Query: 443 EKKAMAEKIERMELDADSKD------KNLVELQELYNSQQLLTAELSAKLEKTEKSLEET 496
E ++ +E I+R E+ + K EL+E Y S+ ELS E E SLEE
Sbjct: 246 ENESKSEGIQRDEVKKVMAELGFFCSKESEELEEKYGSK-----ELSELFEDQEPSLEEV 410
Query: 497 EQTLFDLEERHRQANATIKEKEFLISNLLKSEKELVER 534
+Q FD+ + +R +E ++ L E+ +E+
Sbjct: 411 KQA-FDVFDENRDGFIDARELHRVLCVLGLKEEAGIEK 521
>CB827211
Length = 545
Score = 28.9 bits (63), Expect = 4.4
Identities = 20/88 (22%), Positives = 43/88 (48%), Gaps = 7/88 (7%)
Frame = -3
Query: 952 LKGGHYNRIVEIT-------ENAGKCLLNEYMVDEPSCSTPTRRLFNLPSVSSIEELRTP 1004
L GG + ++ ++ ++GK + + ++ EPS S P R + S++ E +P
Sbjct: 297 LSGGRLSAVLPLSGVVSAAFSSSGKRVYSTILLIEPSVSVPIERPPSDMSLNLSSESASP 118
Query: 1005 SFEELLKAFWDAKSQKLANGDVKHIGSY 1032
+F+ + +F LAN + + +G +
Sbjct: 117 NFKSSMSSFKF*LVSSLANFNRRELGLF 34
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.311 0.127 0.341
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,209,866
Number of Sequences: 28460
Number of extensions: 146245
Number of successful extensions: 641
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 631
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 634
length of query: 1049
length of database: 4,897,600
effective HSP length: 100
effective length of query: 949
effective length of database: 2,051,600
effective search space: 1946968400
effective search space used: 1946968400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 60 (27.7 bits)
Medicago: description of AC149490.9