BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149305.8 - phase: 0 /pseudo
(439 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP039736 57 6e-09
TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, ... 35 0.025
TC19663 similar to UP|Q8H6S8 (Q8H6S8) Translation initiation fac... 34 0.042
TC15611 homologue to UP|Q40209 (Q40209) RAB5A, partial (56%) 32 0.21
BP085186 32 0.27
TC8853 similar to PIR|T09217|T09217 protein sam2B - spinach {Spi... 32 0.27
TC19735 similar to UP|Q8NJM5 (Q8NJM5) Probable ATP-dependent RNA... 32 0.27
TC16752 weakly similar to UP|AAQ96230 (AAQ96230) LRRGT00017, par... 31 0.35
TC18764 similar to UP|Q8LBJ5 (Q8LBJ5) Nucleic acid binding prote... 30 0.60
TC9623 homologue to UP|Q8H1K8 (Q8H1K8) Stromal ascorbate peroxid... 29 1.3
TC8917 similar to UP|AAH64262 (AAH64262) MGC76273 protein, parti... 29 1.3
TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%) 29 1.3
TC14820 homologue to UP|HS80_LYCES (P36181) Heat shock cognate p... 29 1.3
TC18517 weakly similar to UP|AAQ82688 (AAQ82688) Epa4p, partial ... 29 1.8
AW720070 28 2.3
BP034655 28 3.0
TC11270 similar to UP|Q9LM88 (Q9LM88) F2D10.15, partial (9%) 28 3.0
AV776074 28 3.0
BI420393 28 3.0
BF177554 28 3.9
>BP039736
Length = 455
Score = 57.0 bits (136), Expect = 6e-09
Identities = 37/79 (46%), Positives = 48/79 (59%)
Frame = -3
Query: 361 LR*KKTQSLLT*NFIVI*GQLVALNLIIRWLKN*V**NNRKWKWKGNKSWLKRKRLGD*G 420
L+*+K LT*+FIV+ Q +AL+L IR K+* * +N W+ + NKSW KRK+ *G
Sbjct: 453 LQ*RKLLYRLT*SFIVMCEQWIALSLTIR*QKS*A*WSNTNWRKRDNKSWKKRKKSEG*G 274
Query: 421 RNSFLKRSQCRILTDHLFQ 439
N K S C IL L Q
Sbjct: 273 GNLCQKHSPCHILIGLLSQ 217
>TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, partial
(31%)
Length = 727
Score = 35.0 bits (79), Expect = 0.025
Identities = 32/112 (28%), Positives = 49/112 (43%)
Frame = +1
Query: 92 ESQEGFFEKENFFDGEEKNDEEKGENVLEEVIEANLIIHDIGNEERKIDEEEGVEEENEE 151
+S E F+ +N + E + EE E V EEV E EE + +EEEG EEE EE
Sbjct: 139 QSAELPFKLQNKLEAEVQEYEEVEEEVEEEVEE---------EEEEEEEEEEGEEEEEEE 291
Query: 152 KGNCSSMVKRRREKVMEEARNSVPENGKVMHLVKAFERLLSIKKEKEKNEEE 203
+ E+V EA E + + E+++S+ + N +
Sbjct: 292 E---------EEEEVEAEAEEEDDEPIQKLLEPMGKEQIISLLADAASNHRD 420
>TC19663 similar to UP|Q8H6S8 (Q8H6S8) Translation initiation factor,
partial (10%)
Length = 485
Score = 34.3 bits (77), Expect = 0.042
Identities = 41/161 (25%), Positives = 62/161 (38%)
Frame = +3
Query: 44 PQKRIRQRKFVVAKKKKNDQSPRKTVLCKCGENDDGSKCVCEAYRNLRESQEGFFEKENF 103
P++ R KKKK + +K G A + E K+N
Sbjct: 6 PRRL*RSAAVQKKKKKKEKEKEKKAAAAAAGS--------APANETVEVKAEVVESKKN- 158
Query: 104 FDGEEKNDEEKGENVLEEVIEANLIIHDIGNEERKIDEEEGVEEENEEKGNCSSMVKRRR 163
+ + K ++K + E+ EA L E +K +EEE + +E EE+ +
Sbjct: 159 -ESKTKAADKKLPKHVREMQEA-LARRKEAEERKKREEEEKLRKEEEERRRQEEL----- 317
Query: 164 EKVMEEARNSVPENGKVMHLVKAFERLLSIKKEKEKNEEEE 204
E+ EEAR E K L K E L K+KE+ E
Sbjct: 318 ERQAEEARRRKKEREKEKLLKKKQEGKLLTGKQKEEQRRLE 440
>TC15611 homologue to UP|Q40209 (Q40209) RAB5A, partial (56%)
Length = 463
Score = 32.0 bits (71), Expect = 0.21
Identities = 19/42 (45%), Positives = 24/42 (56%)
Frame = -2
Query: 111 DEEKGENVLEEVIEANLIIHDIGNEERKIDEEEGVEEENEEK 152
+E KGE +LEE IGN++ DEEE EEE EE+
Sbjct: 108 EEWKGEKLLEE---------GIGNQKMMKDEEEEEEEEEEEE 10
>BP085186
Length = 388
Score = 31.6 bits (70), Expect = 0.27
Identities = 20/66 (30%), Positives = 29/66 (43%)
Frame = -1
Query: 114 KGENVLEEVIEANLIIHDIGNEERKIDEEEGVEEENEEKGNCSSMVKRRREKVMEEARNS 173
+G N ++ LI D + E +EEE EEE EE+ + EK EE N+
Sbjct: 334 EGSNPYARIVNGKLISPDNADGEGNEEEEEEEEEEEEEEEEEEEEAEPEEEKNEEENVNT 155
Query: 174 VPENGK 179
G+
Sbjct: 154 QEGEGE 137
>TC8853 similar to PIR|T09217|T09217 protein sam2B - spinach {Spinacia
oleracea;}, partial (43%)
Length = 1157
Score = 31.6 bits (70), Expect = 0.27
Identities = 38/140 (27%), Positives = 57/140 (40%), Gaps = 11/140 (7%)
Frame = +3
Query: 41 VVSPQKRIRQRKFVVAKKKKNDQSP-RKTVLCKCGENDDG----SKCVCEAYRNLRESQE 95
+ SP+ I +RK +A P ++ C C DG S+ V + +
Sbjct: 45 IASPRSLISERKPRLAGADALLSLPLKRDSACSCSYPSDGELSRSRVVVARGKKSNSEKN 224
Query: 96 GFF-----EKENFFDGEE-KNDEEKGENVLEEVIEANLIIHDIGNEERKIDEEEGVEEEN 149
G E F+DG+E K + + EE E + ++ N+ D EEG EE
Sbjct: 225 GVDFNASDEDGGFWDGDEWKKEMRRRVGEFEERRELEKMAEELQNQVDD-DGEEGKEETE 401
Query: 150 EEKGNCSSMVKRRREKVMEE 169
EEK VK+ KV +E
Sbjct: 402 EEK---RMRVKKELVKVAKE 452
>TC19735 similar to UP|Q8NJM5 (Q8NJM5) Probable ATP-dependent RNA helicase,
partial (5%)
Length = 565
Score = 31.6 bits (70), Expect = 0.27
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +3
Query: 131 DIGNEERKIDEEEGVEEENEEKGNCSSMVKRRREKVMEEAR 171
D+ NEE +EEE + ++ G S K+++ KV E R
Sbjct: 222 DVANEEHAEEEEEQIGNNSDRDGESSKKKKKKKHKVEVEER 344
>TC16752 weakly similar to UP|AAQ96230 (AAQ96230) LRRGT00017, partial (23%)
Length = 525
Score = 31.2 bits (69), Expect = 0.35
Identities = 19/62 (30%), Positives = 32/62 (50%)
Frame = +3
Query: 88 RNLRESQEGFFEKENFFDGEEKNDEEKGENVLEEVIEANLIIHDIGNEERKIDEEEGVEE 147
RN+R +E + K +G+E+ EK + E + E I ++ NEE + E +E+
Sbjct: 96 RNIRLRREYLYRKS--LEGKERLLYEKKRKIREALQEGKAIPTELRNEEAALRREIDLED 269
Query: 148 EN 149
EN
Sbjct: 270 EN 275
>TC18764 similar to UP|Q8LBJ5 (Q8LBJ5) Nucleic acid binding protein-like,
partial (35%)
Length = 848
Score = 30.4 bits (67), Expect = 0.60
Identities = 16/53 (30%), Positives = 28/53 (52%)
Frame = +1
Query: 100 KENFFDGEEKNDEEKGENVLEEVIEANLIIHDIGNEERKIDEEEGVEEENEEK 152
KE+ + K++ ++G LI D E + ++EEG+EEE+EE+
Sbjct: 82 KESHSSNKPKSNSKRGSEPQXVKYSKGLISKDEDEGEGEDEDEEGLEEEDEEE 240
>TC9623 homologue to UP|Q8H1K8 (Q8H1K8) Stromal ascorbate peroxidase,
partial (42%)
Length = 640
Score = 29.3 bits (64), Expect = 1.3
Identities = 11/18 (61%), Positives = 16/18 (88%)
Frame = -1
Query: 194 KKEKEKNEEEEENDKKNK 211
K+E+E+ EEEEEN+KK +
Sbjct: 196 KEEREEEEEEEENEKKER 143
Score = 26.6 bits (57), Expect = 8.7
Identities = 17/44 (38%), Positives = 23/44 (51%)
Frame = -1
Query: 131 DIGNEERKIDEEEGVEEENEEKGNCSSMVKRRREKVMEEARNSV 174
++ EERK +EE EEE EE+ K+ RE+ EE V
Sbjct: 226 EMRGEERKHWKEEREEEEEEEENE-----KKEREEYEEEESERV 110
>TC8917 similar to UP|AAH64262 (AAH64262) MGC76273 protein, partial (3%)
Length = 676
Score = 29.3 bits (64), Expect = 1.3
Identities = 29/100 (29%), Positives = 43/100 (43%), Gaps = 3/100 (3%)
Frame = +2
Query: 107 EEKNDEEKGENVLEEVIEANLIIHDIGNEERKIDEEEGVEEENEEKGNCSSMVKRRRE-- 164
E KN+E K E V E EAN+ E++ E NE+ + S+++ E
Sbjct: 179 EIKNEEVKVEQVQES--EANV-------------EKKTEETPNEDNKSLSTLLNENEEEA 313
Query: 165 -KVMEEARNSVPENGKVMHLVKAFERLLSIKKEKEKNEEE 203
K EE + E VK + ++ K E+E EEE
Sbjct: 314 KKPEEEVQAEPKEEKATTEEVKVETKEVNPKPEEEPKEEE 433
Score = 27.7 bits (60), Expect = 3.9
Identities = 28/95 (29%), Positives = 38/95 (39%)
Frame = +2
Query: 108 EKNDEEKGENVLEEVIEANLIIHDIGNEERKIDEEEGVEEENEEKGNCSSMVKRRREKVM 167
E N E+K E E ++ + + EE K EEE E EEK + KV
Sbjct: 224 EANVEKKTEETPNEDNKSLSTLLNENEEEAKKPEEEVQAEPKEEKATTEEV------KVE 385
Query: 168 EEARNSVPENGKVMHLVKAFERLLSIKKEKEKNEE 202
+ N PE K E +++EK K EE
Sbjct: 386 TKEVNPKPEEEP-----KEEEPKAQVQEEKPKTEE 475
>TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%)
Length = 1008
Score = 29.3 bits (64), Expect = 1.3
Identities = 19/53 (35%), Positives = 28/53 (51%)
Frame = -2
Query: 99 EKENFFDGEEKNDEEKGENVLEEVIEANLIIHDIGNEERKIDEEEGVEEENEE 151
E E DGE++ D++ + +E D G E+ +EEEGVEEE+ E
Sbjct: 431 EDEEDEDGEDQEDDDDDDEDDDE--------EDDGGED---EEEEGVEEEDNE 306
>TC14820 homologue to UP|HS80_LYCES (P36181) Heat shock cognate protein 80,
partial (53%)
Length = 1180
Score = 29.3 bits (64), Expect = 1.3
Identities = 32/130 (24%), Positives = 59/130 (44%), Gaps = 3/130 (2%)
Frame = +1
Query: 89 NLRESQEGFFEKENFFDGEEKNDEEKGENV---LEEVIEANLIIHDIGNEERKIDEEEGV 145
+L+E Q + E+ D +K+ E + +E+ E I D +EE K DEE V
Sbjct: 604 HLKEDQLEYLEERRLKDLIKKHSEFISYPISLWIEKTTEKE--ISDDEDEEEKKDEEGKV 777
Query: 146 EEENEEKGNCSSMVKRRREKVMEEARNSVPENGKVMHLVKAFERLLSIKKEKEKNEEEEE 205
E+ +EEK +++++K+ E + N ++ + ++K +E N+EE
Sbjct: 778 EDVDEEK----EKEEKKKKKIKEVSHEWSLVNK---------QKPIWMRKPEEINKEEYA 918
Query: 206 NDKKNKVMKW 215
K+ W
Sbjct: 919 AFYKSLTNDW 948
>TC18517 weakly similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (4%)
Length = 493
Score = 28.9 bits (63), Expect = 1.8
Identities = 27/96 (28%), Positives = 45/96 (46%), Gaps = 5/96 (5%)
Frame = -2
Query: 58 KKKNDQSPRK----TVLCKCGENDDGSKCVCEAYRNLRESQEGFFEKENFFD-GEEKNDE 112
++ N +P + TV+ GE++D + E E E + + D G+E+++E
Sbjct: 465 ERNNGNNPERGEDETVIDGAGEDNDRAVAEEEEEEPGEEDGEEDDDGDGVPDEGDEEDEE 286
Query: 113 EKGENVLEEVIEANLIIHDIGNEERKIDEEEGVEEE 148
E V EEV A +++H G + EGVE E
Sbjct: 285 EDHGVVAEEV--AEVVLHAEGGFAEGVRAGEGVEGE 184
>AW720070
Length = 484
Score = 28.5 bits (62), Expect = 2.3
Identities = 15/40 (37%), Positives = 22/40 (54%)
Frame = +1
Query: 134 NEERKIDEEEGVEEENEEKGNCSSMVKRRREKVMEEARNS 173
++ R+ +EEE EEE EE G + R E+ EE +S
Sbjct: 97 SDSREEEEEEEEEEEEEEAGEEGIITTTREEEEEEEESSS 216
Score = 28.1 bits (61), Expect = 3.0
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +1
Query: 133 GNEERKIDEEEGVEEENEEKGNCSSMVKRRREKVMEEARNS 173
G + + +EEE EEE EE+ ++ RE+ EE +S
Sbjct: 91 GYSDSREEEEEEEEEEEEEEAGEEGIITTTREEEEEEEESS 213
Score = 26.6 bits (57), Expect = 8.7
Identities = 17/55 (30%), Positives = 25/55 (44%)
Frame = +1
Query: 101 ENFFDGEEKNDEEKGENVLEEVIEANLIIHDIGNEERKIDEEEGVEEENEEKGNC 155
+ + D E+ +EE+ E EE E +I EEE EEE+ K +C
Sbjct: 88 KGYSDSREEEEEEEEEEEEEEAGEEGIIT--------TTREEEEEEEESSSKHHC 228
>BP034655
Length = 517
Score = 28.1 bits (61), Expect = 3.0
Identities = 25/108 (23%), Positives = 47/108 (43%), Gaps = 10/108 (9%)
Frame = +1
Query: 121 EVIEANLIIHDIG-----NEERKIDEEEGVEEENEEKGNCSSMVKRRREKVMEEARNSVP 175
E+ A+L D G EE + +EEE EEE EE+ + E+ +E V
Sbjct: 91 EIAAAHLTAADFGLSDDDEEEEEEEEEEEEEEEEEEEEEEEEEDEEDDEEDEDEDYIPVA 270
Query: 176 ENGKV-----MHLVKAFERLLSIKKEKEKNEEEEENDKKNKVMKWALP 218
+ +V + ++ I +++N EE+ +K+ ++ + P
Sbjct: 271 PSHRVSPNVHQNPTSDSVEVILIPDSEQQNYSEEDGNKRRRIEEGEAP 414
>TC11270 similar to UP|Q9LM88 (Q9LM88) F2D10.15, partial (9%)
Length = 735
Score = 28.1 bits (61), Expect = 3.0
Identities = 17/38 (44%), Positives = 22/38 (57%), Gaps = 1/38 (2%)
Frame = +3
Query: 107 EEKNDEEKGENVLEEVIEANLIIHDI-GNEERKIDEEE 143
EE +EE GE EE E +++ D GNE+ DEEE
Sbjct: 423 EEDEEEENGE---EEEEEEEVVVEDEQGNEDEDEDEEE 527
>AV776074
Length = 391
Score = 28.1 bits (61), Expect = 3.0
Identities = 16/43 (37%), Positives = 22/43 (50%)
Frame = +1
Query: 134 NEERKIDEEEGVEEENEEKGNCSSMVKRRREKVMEEARNSVPE 176
++ER + EEE EE + +G + KRRR K R PE
Sbjct: 73 SQERPMGEEEKKAEEKKSRGPTRAREKRRRRK--NRRRRQKPE 195
>BI420393
Length = 515
Score = 28.1 bits (61), Expect = 3.0
Identities = 16/43 (37%), Positives = 25/43 (57%), Gaps = 11/43 (25%)
Frame = +1
Query: 121 EVIEANLIIHDIGN--------EERKID---EEEGVEEENEEK 152
E++E ++HDIG+ EE+ ++ EEEGVEE + K
Sbjct: 100 EIVELAALLHDIGDYKYLMDPSEEKIVENFLEEEGVEENMKSK 228
>BF177554
Length = 246
Score = 27.7 bits (60), Expect = 3.9
Identities = 19/50 (38%), Positives = 26/50 (52%)
Frame = +1
Query: 104 FDGEEKNDEEKGENVLEEVIEANLIIHDIGNEERKIDEEEGVEEENEEKG 153
F E+ DE+ E EE E++ EE + +EEEG EEE E+ G
Sbjct: 4 FGTSEQQDEDSCE---EEAGESS--------EEEEEEEEEGEEEEEEDDG 120
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.339 0.149 0.490
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,634,078
Number of Sequences: 28460
Number of extensions: 107808
Number of successful extensions: 1324
Number of sequences better than 10.0: 65
Number of HSP's better than 10.0 without gapping: 1148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1237
length of query: 439
length of database: 4,897,600
effective HSP length: 93
effective length of query: 346
effective length of database: 2,250,820
effective search space: 778783720
effective search space used: 778783720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC149305.8