BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149212.6 - phase: 0 /pseudo
(1684 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP052632 99 6e-21
BP066094 82 7e-16
BP084332 76 4e-14
AV427921 56 4e-08
AU088980 55 7e-08
BP063694 43 5e-04
BP085942 37 0.003
TC14791 similar to UP|Q9LJ04 (Q9LJ04) ESTs AU082210(C53655), par... 37 0.034
TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase regi... 36 0.044
TC14792 similar to UP|O73620 (O73620) Nuclear protein SDK2, part... 34 0.22
BP055130 32 0.63
TC17878 similar to GB|AAK91488.1|15215889|AY050475 AT5g02770/F9G... 32 0.63
AV415593 32 0.83
TC9358 weakly similar to GB|AAH10781.1|14789753|BC010781 RIO kin... 32 1.1
BP033602 31 1.4
BP063926 30 2.4
TC8273 UP|BAD11134 (BAD11134) Glutamate-rich protein, complete 30 4.1
TC20101 29 5.4
>BP052632
Length = 489
Score = 99.0 bits (245), Expect = 6e-21
Identities = 54/120 (45%), Positives = 72/120 (60%), Gaps = 2/120 (1%)
Frame = -3
Query: 934 KTAYELFKGTRPNISYFHQFGCTCYILNTKDYLKKFDAKAQRGIFLGYSERSKAYRVYNS 993
KT YEL++G RP +SYFH FGC +ILNT D + KFD K + I LGYS SKAY V+NS
Sbjct: 487 KTPYELWRGRRPTVSYFHPFGCK*FILNTNDNIGKFDNKFDKRILLGYSSNSKAYIVFNS 308
Query: 994 ETHCVEESMHVKFDD-REPGSKTPEQSESNAGTIDSEDASESDQLSDSE-KHSEVESSPE 1051
T VEES++VKFDD + E+ N D ++ +++ SE H E + P+
Sbjct: 307 RTQVVEESINVKFDD*SIEATYRLEKDFLNLDLSDQDEEVPTNEGQPSETSHEEPSTQPQ 128
>BP066094
Length = 532
Score = 82.0 bits (201), Expect = 7e-16
Identities = 60/132 (45%), Positives = 77/132 (57%)
Frame = +3
Query: 1265 KQSGYFYPMQLIMA*YCIKWMSKVVLLMVSLKKKFMSNNLLGLRILSILTMFINLRNHYM 1324
K S Y Q I *+ I+ M +V MV+ ++K M N ++ LT+F N RN M
Sbjct: 96 KLSDY*SHSQ*ITT*FFIR*MLRVPF*MVTSQRKSMFINPQVXKMRRTLTIFSN*RNLSM 275
Query: 1325 A*NKLPELGMID*VIS*LKMILKEDKLTQHSSEGLLRKTF*LCKYMLMI*YLVLLMHLFA 1384
*+KLPE GM D S KM K Q S L + +*LCK+MLMI YL LL+HL+A
Sbjct: 276 V*SKLPEHGMRDSAHSFWKMSX*GVK*IQLSFAKLTKMIY*LCKFMLMILYLDLLIHLYA 455
Query: 1385 KNFLS*CRMNLK 1396
K+FL *CR+NLK
Sbjct: 456 KSFLR*CRLNLK 491
>BP084332
Length = 368
Score = 76.3 bits (186), Expect = 4e-14
Identities = 47/109 (43%), Positives = 64/109 (58%)
Frame = +1
Query: 1567 NYCYVYSRSRIHFSCKLLYTTTLDETSVGRLSD*C*QYSHFL**YCCYLSVKESNSTFKS 1626
N+C +Y R RI+ S + ++ LDETS G LS+ QY + L**+CC ES TFK
Sbjct: 37 NHCTIYCRGRIYLSSNMQHSDALDETSAGGLSNP*EQYPNLL**HCCNFIE*ESYLTFKG 216
Query: 1627 QAY*DQTPFYQRLCSKRDFRYTIH*Y*TSMG*YIYKTFIG*KI*FY*EK 1675
+A+* + Y RLC++ + *Y* SMG Y+YK I*F+ EK
Sbjct: 217 KAH*GKVSLY*RLCTEGRTSSEVC*Y*PSMGRYLYKALSRG*I*FHSEK 363
>AV427921
Length = 284
Score = 56.2 bits (134), Expect = 4e-08
Identities = 30/79 (37%), Positives = 42/79 (52%)
Frame = +1
Query: 880 TPQQNGVVERKNRTLQEMARTMIHENNLAKHFWAEAINTSCYIQNRIYIRPMLEKTAYEL 939
TPQQNGV ERKNRT+ M R+++ + L K F EA+ S +I NR + E
Sbjct: 22 TPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQNMMPEEA 201
Query: 940 FKGTRPNISYFHQFGCTCY 958
+ G +P + +F C Y
Sbjct: 202 WSGRQPAVDHFRISRCLAY 258
>AU088980
Length = 360
Score = 55.5 bits (132), Expect = 7e-08
Identities = 28/112 (25%), Positives = 52/112 (46%)
Frame = +2
Query: 884 NGVVERKNRTLQEMARTMIHENNLAKHFWAEAINTSCYIQNRIYIRPMLEKTAYELFKGT 943
N +VERK+R + + R ++ + L K+ W A+ + Y+ N + + + Y+
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAYLINXLPSPLLKGQCPYQFLNND 181
Query: 944 RPNISYFHQFGCTCYILNTKDYLKKFDAKAQRGIFLGYSERSKAYRVYNSET 995
P + FG CY +K D +A++ +FLG+ + Y V + T
Sbjct: 182 SPTLLDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKTGTXGYIVMDLPT 337
>BP063694
Length = 511
Score = 42.7 bits (99), Expect = 5e-04
Identities = 25/75 (33%), Positives = 37/75 (49%)
Frame = -2
Query: 760 ACQKGKIVKSSFKSKDIVSTSRPLELLHIDLFGQVNTTSLYGSKYGLVIVDDYSRWTWVK 819
+CQ K + F + S L+L+H DL G S+ G Y ++ VDD+SR+TW
Sbjct: 252 SCQMAKSKRLVFHDNN-KRASAVLDLIHCDLRGPSPVASIDGFSYFVIFVDDFSRFTWFY 76
Query: 820 FIKSKDYACEVFSSF 834
+K K +V F
Sbjct: 75 PLKRKSDFSDVLLRF 31
>BP085942
Length = 208
Score = 36.6 bits (83), Expect(2) = 0.003
Identities = 20/61 (32%), Positives = 33/61 (53%)
Frame = +3
Query: 802 SKYGLVIVDDYSRWTWVKFIKSKDYACEVFSSFCTQIQSEKELKILKVRSDHGGEFENET 861
SK+ +V++D +I KD F++F TQ+Q E + ++ VRSDHG ++
Sbjct: 6 SKHRVVLLDQ-------NWILHKDETHTRFTNFITQVQKEFQTSVITVRSDHGWRI*EQS 164
Query: 862 F 862
F
Sbjct: 165 F 167
Score = 22.7 bits (47), Expect(2) = 0.003
Identities = 9/21 (42%), Positives = 12/21 (56%)
Frame = +1
Query: 854 GGEFENETFESFCEKHGILHE 874
GGEFEN+ E + G L +
Sbjct: 142 GGEFENKALEEWFNSQGNLSQ 204
>TC14791 similar to UP|Q9LJ04 (Q9LJ04) ESTs AU082210(C53655), partial (37%)
Length = 1779
Score = 36.6 bits (83), Expect = 0.034
Identities = 22/85 (25%), Positives = 42/85 (48%), Gaps = 2/85 (2%)
Frame = +2
Query: 1004 VKFDDREPGSKTP--EQSESNAGTIDSEDASESDQLSDSEKHSEVESSPEAEITPEAESN 1061
V+ D ++P + P + +SN E+ E DQ ++E +++ + E E PEA +
Sbjct: 143 VELDHQQPPNPVPTTDLDQSNNTMAVDEEPEEQDQ--ETEDQPQIDDAVEEEQDPEAPAT 316
Query: 1062 SEAEPSPEIQNENASEDIQDNSQQA 1086
+E+EP + + + D + S A
Sbjct: 317 TESEPQQQQETLEDNADAPNESSAA 391
>TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase region
(Fragment), partial (10%)
Length = 572
Score = 36.2 bits (82), Expect = 0.044
Identities = 16/53 (30%), Positives = 30/53 (56%)
Frame = +2
Query: 852 DHGGEFENETFESFCEKHGILHEFSSPRTPQQNGVVERKNRTLQEMARTMIHE 904
++G +F ++ + FC+ GI FSS + PQ NG E N+ + + + ++E
Sbjct: 8 ENGIQFTSKQTQDFCDGMGIQMRFSSVKHPQTNGQTEAANKVILKGIKRRLYE 166
>TC14792 similar to UP|O73620 (O73620) Nuclear protein SDK2, partial (5%)
Length = 492
Score = 33.9 bits (76), Expect = 0.22
Identities = 19/68 (27%), Positives = 36/68 (52%), Gaps = 2/68 (2%)
Frame = +2
Query: 1004 VKFDDREPGSKTP--EQSESNAGTIDSEDASESDQLSDSEKHSEVESSPEAEITPEAESN 1061
V+ D ++P + P + +SN E+ E DQ ++E +++ + E E PEA +
Sbjct: 158 VELDHQQPPNPEPTTDLDQSNNTMAVDEEPEEQDQ--ETEDQPQIDDAVEEEQDPEAPAT 331
Query: 1062 SEAEPSPE 1069
+E+EP +
Sbjct: 332 TESEPQQQ 355
>BP055130
Length = 567
Score = 32.3 bits (72), Expect = 0.63
Identities = 25/99 (25%), Positives = 44/99 (44%)
Frame = +2
Query: 806 LVIVDDYSRWTWVKFIKSKDYACEVFSSFCTQIQSEKELKILKVRSDHGGEFENETFESF 865
+V+VD S++ ++ + +V +F + I + V SD F + ++ F
Sbjct: 194 IVVVDRLSKYGHFAPHRANYTSSQVAETFVSTIVKLHGMPRAIV-SDRDKAFTSAFWKHF 370
Query: 866 CEKHGILHEFSSPRTPQQNGVVERKNRTLQEMARTMIHE 904
+ HG SS PQ +G E N+ L+ R +HE
Sbjct: 371 FKLHGTTLNMSSSYHPQTDGQTEALNKCLELYLRCFVHE 487
>TC17878 similar to GB|AAK91488.1|15215889|AY050475 AT5g02770/F9G14_80
{Arabidopsis thaliana;}, partial (17%)
Length = 465
Score = 32.3 bits (72), Expect = 0.63
Identities = 21/77 (27%), Positives = 38/77 (49%), Gaps = 1/77 (1%)
Frame = +2
Query: 1011 PGSKTPEQSESNAGTIDSEDASESDQLSDSEKHSEVESSPEAEITP-EAESNSEAEPSPE 1069
P TP Q N T++ E+ ++E+ + +S P+++ P AE +E EP PE
Sbjct: 77 PNDTTPTQENPNK-TLEPEE--------EAEEPTPPQSEPQSQPDPIAAEPETEPEPEPE 229
Query: 1070 IQNENASEDIQDNSQQA 1086
++ D+Q ++A
Sbjct: 230 PASDAPLSDVQKKMRRA 280
>AV415593
Length = 416
Score = 32.0 bits (71), Expect = 0.83
Identities = 26/94 (27%), Positives = 42/94 (44%)
Frame = +3
Query: 1000 ESMHVKFDDREPGSKTPEQSESNAGTIDSEDASESDQLSDSEKHSEVESSPEAEITPEAE 1059
ES H DD E S+ + S S + D E+ S E+H + E +AE EAE
Sbjct: 3 ESPHRSSDDSENASENVDVSGSESA--DGEECSR-------EEHEDREHDNKAESEGEAE 155
Query: 1060 SNSEAEPSPEIQNENASEDIQDNSQQAIQPKFKH 1093
+A +++ + S I + Q ++P K+
Sbjct: 156 GMVDAH---DVEGDGMSLTISERFLQTVKPLAKY 248
>TC9358 weakly similar to GB|AAH10781.1|14789753|BC010781 RIO kinase 2 {Mus
musculus;} , partial (10%)
Length = 1117
Score = 31.6 bits (70), Expect = 1.1
Identities = 24/112 (21%), Positives = 48/112 (42%)
Frame = +1
Query: 1010 EPGSKTPEQSESNAGTIDSEDASESDQLSDSEKHSEVESSPEAEITPEAESNSEAEPSPE 1069
E GS + + G++D E A+ D+E E TPE++SNS++E
Sbjct: 382 EDGSPCLSEIDRATGSLDRELAASGFTKKDAEDIQRF-----IEGTPESDSNSDSEGVDS 546
Query: 1070 IQNENASEDIQDNSQQAIQPKFKHKSSHLRS*SLEAKIVLEEQDHISDKKSH 1121
+++ N + D+ + +H+S + + ++ SD++ H
Sbjct: 547 VEDLNEAIIKDDDFSYLSEQDDEHESKGIEESCEAGQSYASDKGDASDEEEH 702
>BP033602
Length = 533
Score = 31.2 bits (69), Expect = 1.4
Identities = 11/34 (32%), Positives = 22/34 (64%)
Frame = +3
Query: 780 SRPLELLHIDLFGQVNTTSLYGSKYGLVIVDDYS 813
SRP L+H D++G T+ +G ++ + +DD++
Sbjct: 432 SRPFYLIHSDVWGPSKITTQFGKRWFVTFIDDHT 533
>BP063926
Length = 487
Score = 30.4 bits (67), Expect = 2.4
Identities = 22/80 (27%), Positives = 34/80 (42%), Gaps = 5/80 (6%)
Frame = +2
Query: 1025 TIDSEDA----SESDQLSDSEKHSEVESSPEAEITPEAESNSEAE-PSPEIQNENASEDI 1079
T+D D+ S LSD+ HS+ + P+ + P AE++ EA S E + E
Sbjct: 116 TVDDSDSDFEVSSDATLSDTASHSDSDDDPQTQNPPVAEADPEAHYHSSEQEYEEEGSFE 295
Query: 1080 QDNSQQAIQPKFKHKSSHLR 1099
D ++ H LR
Sbjct: 296 ADEYSPLVESPGSHGGIPLR 355
>TC8273 UP|BAD11134 (BAD11134) Glutamate-rich protein, complete
Length = 923
Score = 29.6 bits (65), Expect = 4.1
Identities = 18/72 (25%), Positives = 35/72 (48%)
Frame = +1
Query: 1007 DDREPGSKTPEQSESNAGTIDSEDASESDQLSDSEKHSEVESSPEAEITPEAESNSEAEP 1066
+ P +T E+ + A +E +E+ ++E +EVE+ E+T E + +E
Sbjct: 196 EQEHPKEETTEEPKVEAV---AETTTEAPAAPETEAPAEVETK---EVTEETKPEAEKTE 357
Query: 1067 SPEIQNENASED 1078
PE++ E E+
Sbjct: 358 EPEVKPEEVKEE 393
>TC20101
Length = 513
Score = 29.3 bits (64), Expect = 5.4
Identities = 16/38 (42%), Positives = 20/38 (52%)
Frame = +2
Query: 783 LELLHIDLFGQVNTTSLYGSKYGLVIVDDYSRWTWVKF 820
L + H G TT+LYG + VIVD Y +W KF
Sbjct: 152 LRVSH*PSLGVDITTTLYGVRVYGVIVDSYGQWCHGKF 265
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.359 0.159 0.563
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,817,555
Number of Sequences: 28460
Number of extensions: 358772
Number of successful extensions: 3849
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 2789
Number of HSP's successfully gapped in prelim test: 97
Number of HSP's that attempted gapping in prelim test: 991
Number of HSP's gapped (non-prelim): 2990
length of query: 1684
length of database: 4,897,600
effective HSP length: 103
effective length of query: 1581
effective length of database: 1,966,220
effective search space: 3108593820
effective search space used: 3108593820
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.8 bits)
S2: 62 (28.5 bits)
Medicago: description of AC149212.6