BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149206.7 + phase: 0
(310 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8358 similar to UP|Q84T14 (Q84T14) Vacuolar ATPase subunit E (... 36 0.007
CB826758 36 0.007
TC15001 similar to UP|GLE1_YEAST (Q12315) RNA export factor GLE1... 32 0.10
AV408585 29 1.2
TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, ... 29 1.2
TC16374 similar to UP|Q7LZA6 (Q7LZA6) Protamine II (Fragment), p... 29 1.2
TC10393 29 1.2
TC12218 28 1.5
TC11276 weakly similar to UP|HDA1_CHICK (P56517) Histone deacety... 28 2.6
TC15746 28 2.6
TC15620 similar to UP|Q940X9 (Q940X9) AT5g21160/T10F18_190 (Prol... 27 3.4
AV770669 27 3.4
TC12355 similar to UP|PR18_HUMAN (Q99633) Pre-mRNA splicing fact... 27 3.4
TC12553 similar to GB|AAN31120.1|23506217|AY149966 At2g17220/T23... 27 4.4
TC14500 UP|Q9S7B1 (Q9S7B1) Nodule INCEPTION protein, complete 27 4.4
TC13558 similar to GB|AAD33770.1|4928919|AF138744 zinc finger pr... 27 4.4
TC14415 similar to UP|O04237 (O04237) Transcription factor, part... 27 4.4
AI967601 27 5.8
TC18261 similar to UP|WSC2_YEAST (P53832) Cell wall integrity an... 27 5.8
BP034563 27 5.8
>TC8358 similar to UP|Q84T14 (Q84T14) Vacuolar ATPase subunit E (Fragment),
complete
Length = 1321
Score = 36.2 bits (82), Expect = 0.007
Identities = 22/66 (33%), Positives = 39/66 (58%)
Frame = +1
Query: 93 ESKEKEFEIRVMEFQSKEEEFKVQVKVLFEAKEEKFEVKMQQFENQVEDNLKSVKALELK 152
E++EK EI V S EEEF ++ L EA+++K + ++ E Q+E K +++L
Sbjct: 145 EAEEKANEISV----SAEEEFNIEKLQLVEAEKKKIRQEYERKERQIEIRRKIEYSMQLN 312
Query: 153 ENQIEV 158
++I+V
Sbjct: 313 ASRIKV 330
>CB826758
Length = 537
Score = 36.2 bits (82), Expect = 0.007
Identities = 21/94 (22%), Positives = 50/94 (52%), Gaps = 1/94 (1%)
Frame = +2
Query: 22 EERRLRSI-KRDIEKCCEELENMETQVKEFETEKNILDGQVNEFESKKGELEGLLRDFES 80
EE LR + + ++E EELEN+++Q + + E ++ Q + ES+ E ++++ E
Sbjct: 155 EELNLRKMAEEELETEKEELENLKSQADKVKEELRLVQDQKSSLESQLASSELMVKELEQ 334
Query: 81 EKTNFERRQKEFESKEKEFEIRVMEFQSKEEEFK 114
+ + + ++++ +E E++ + EE +
Sbjct: 335 KIISAVDLLQNYKNEREELEVQRDSLLREAEELR 436
Score = 28.1 bits (61), Expect = 2.0
Identities = 30/114 (26%), Positives = 52/114 (45%), Gaps = 4/114 (3%)
Frame = +2
Query: 3 RKELASLKKSFAECNGQQVEERRLRSIKRDIEKCCEELENMETQVKEFE----TEKNILD 58
++EL +LK ++ + + E R ++ K +E +L + E VKE E + ++L
Sbjct: 203 KEELENLK---SQADKVKEELRLVQDQKSSLES---QLASSELMVKELEQKIISAVDLLQ 364
Query: 59 GQVNEFESKKGELEGLLRDFESEKTNFERRQKEFESKEKEFEIRVMEFQSKEEE 112
NE E + + + LLR+ E E R+K+ E+ EF E E
Sbjct: 365 NYKNEREELEVQRDSLLREAE------ELRKKQGEASSSHVSQLFSEFSFSEIE 508
>TC15001 similar to UP|GLE1_YEAST (Q12315) RNA export factor GLE1, partial
(4%)
Length = 988
Score = 32.3 bits (72), Expect = 0.10
Identities = 36/139 (25%), Positives = 62/139 (43%), Gaps = 10/139 (7%)
Frame = +2
Query: 21 VEERRLRSIKRDIEKCCEELEN-----METQVKEFETEKNILDGQVNEFESKKGELEGLL 75
+EE + ++ I K EE N +E Q + E K ++ + E +K
Sbjct: 386 IEEETAKRVEEAIRKRVEESLNSEEVQVEIQRRLEEGRKRLIVEVAVQLEKEKEAALIEA 565
Query: 76 RDFES----EKTNFERRQKEFESKEKEFEIR-VMEFQSKEEEFKVQVKVLFEAKEEKFEV 130
R+ E EK + +R +E K +E + R +E Q +EEE +++ + KEE
Sbjct: 566 REKEEQARKEKEDLDRMLEENRKKIEEAQRREALEQQRREEERYRELEEIQRQKEEAMRR 745
Query: 131 KMQQFENQVEDNLKSVKAL 149
K Q+ E ++ L +K L
Sbjct: 746 KKQEEE---QERLNQIKLL 793
Score = 31.6 bits (70), Expect = 0.18
Identities = 26/126 (20%), Positives = 57/126 (44%), Gaps = 1/126 (0%)
Frame = +2
Query: 65 ESKKGELEGLLRDFESEKTNFERRQKEFESKEKEFEIR-VMEFQSKEEEFKVQVKVLFEA 123
E +E +R E N E Q E + + +E R ++E + E+ K +
Sbjct: 392 EETAKRVEEAIRKRVEESLNSEEVQVEIQRRLEEGRKRLIVEVAVQLEKEKEAALIEARE 571
Query: 124 KEEKFEVKMQQFENQVEDNLKSVKALELKENQIEVQIKDLKSKLNNFGGQPKELELTEKQ 183
KEE+ + + + +E+N K ++ + +E + + ++ + + + KE + K+
Sbjct: 572 KEEQARKEKEDLDRMLEENRKKIEEAQRREALEQQRREEERYRELEEIQRQKEEAMRRKK 751
Query: 184 HDEEKE 189
+EE+E
Sbjct: 752 QEEEQE 769
>AV408585
Length = 430
Score = 28.9 bits (63), Expect = 1.2
Identities = 23/79 (29%), Positives = 36/79 (45%), Gaps = 2/79 (2%)
Frame = +2
Query: 20 QVEERRLRSIKRDIEKCCEEL--ENMETQVKEFETEKNILDGQVNEFESKKGELEGLLRD 77
Q+EE ++ SIKRD + E+L E +E E +K + +K+ E R
Sbjct: 152 QIEENKVESIKRD-KTATEKLLQETIEKHQNELAAQKEYYTNAL--AAAKEAEALAEARA 322
Query: 78 FESEKTNFERRQKEFESKE 96
+T E R +E E +E
Sbjct: 323 NNEARTELESRLREAEERE 379
>TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, partial
(31%)
Length = 727
Score = 28.9 bits (63), Expect = 1.2
Identities = 29/99 (29%), Positives = 49/99 (49%), Gaps = 6/99 (6%)
Frame = +1
Query: 58 DGQVNEFESKKGELEGLLRDFESEKTNFERRQK------EFESKEKEFEIRVMEFQSKEE 111
+ VNE +KK +L + + +S + F+ + K E+E E+E E V E + +EE
Sbjct: 82 EAMVNESITKKRKL--VPKSSQSAELPFKLQNKLEAEVQEYEEVEEEVEEEVEEEEEEEE 255
Query: 112 EFKVQVKVLFEAKEEKFEVKMQQFENQVEDNLKSVKALE 150
E + E +EE+ E + + E + ED+ K LE
Sbjct: 256 EEEEGE----EEEEEEEEEEEVEAEAEEEDDEPIQKLLE 360
>TC16374 similar to UP|Q7LZA6 (Q7LZA6) Protamine II (Fragment), partial
(63%)
Length = 1130
Score = 28.9 bits (63), Expect = 1.2
Identities = 24/83 (28%), Positives = 39/83 (46%)
Frame = +3
Query: 39 ELENMETQVKEFETEKNILDGQVNEFESKKGELEGLLRDFESEKTNFERRQKEFESKEKE 98
E+EN E E +TE D + N + KK +G EK N +R K+ K
Sbjct: 600 EIENDEDSETEIQTE----DTEENVQKKKKKNTKG-------EKQNKKRLGKKKSDKSNT 746
Query: 99 FEIRVMEFQSKEEEFKVQVKVLF 121
++R + Q+K+ + +V+ LF
Sbjct: 747 RKMRSTKRQAKKSRKE*EVRCLF 815
>TC10393
Length = 1063
Score = 28.9 bits (63), Expect = 1.2
Identities = 13/58 (22%), Positives = 31/58 (53%)
Frame = +1
Query: 24 RRLRSIKRDIEKCCEELENMETQVKEFETEKNILDGQVNEFESKKGELEGLLRDFESE 81
R + ++ + +K + ++ ++KE +TEKN L + +++K LE L+ ++
Sbjct: 556 RMVTQLRGEAQKMKDTNMGLQEKIKELKTEKNELRDEKQRLKTEKERLEQQLKSMNAQ 729
>TC12218
Length = 737
Score = 28.5 bits (62), Expect = 1.5
Identities = 13/49 (26%), Positives = 28/49 (56%)
Frame = +2
Query: 24 RRLRSIKRDIEKCCEELENMETQVKEFETEKNILDGQVNEFESKKGELE 72
R + ++ + EK E +++ ++KE + EKN + + N+ + K +LE
Sbjct: 401 RVVTQLRNEAEKLKEMNNDLQEKIKELKAEKNEIRDEKNKLKLDKEKLE 547
>TC11276 weakly similar to UP|HDA1_CHICK (P56517) Histone deacetylase 1
(HD1), partial (5%)
Length = 636
Score = 27.7 bits (60), Expect = 2.6
Identities = 21/70 (30%), Positives = 37/70 (52%)
Frame = +3
Query: 79 ESEKTNFERRQKEFESKEKEFEIRVMEFQSKEEEFKVQVKVLFEAKEEKFEVKMQQFENQ 138
+ E+ E QKE E K++ + ++K+EE K + + E KEE+ K ++ Q
Sbjct: 33 KKEEPKKEEPQKEEEKKDEPKSEEPQKEEAKKEEPKKEEEKKDEKKEEE---KKKEQPAQ 203
Query: 139 VEDNLKSVKA 148
++ L+ VKA
Sbjct: 204 IDPVLEWVKA 233
>TC15746
Length = 619
Score = 27.7 bits (60), Expect = 2.6
Identities = 18/77 (23%), Positives = 40/77 (51%), Gaps = 4/77 (5%)
Frame = +2
Query: 135 FENQVEDNLKSVKALELKENQIEVQI----KDLKSKLNNFGGQPKELELTEKQHDEEKEF 190
+E+ V ++V ++ + +++ + ++LKSKLN + +E E T +EE+
Sbjct: 14 YEDTVTRYTENVGDMKSENGDVQLNLAEDSEELKSKLNIMDSKLREAEATIMMLNEERRM 193
Query: 191 DTSYMDDDGASEEIDIL 207
+T + D E+++L
Sbjct: 194 NTK--EKDFFKRELEVL 238
>TC15620 similar to UP|Q940X9 (Q940X9) AT5g21160/T10F18_190 (Proline-rich
protein family), partial (19%)
Length = 1063
Score = 27.3 bits (59), Expect = 3.4
Identities = 24/83 (28%), Positives = 33/83 (38%), Gaps = 6/83 (7%)
Frame = +3
Query: 53 EKNILDGQVNEFES------KKGELEGLLRDFESEKTNFERRQKEFESKEKEFEIRVMEF 106
EK D +FE KG L GL + + R QKE K E E + E
Sbjct: 291 EKEFRDDLYKDFEQLSLDFYHKGNLYGLEKYWAFHHYRKLRNQKEPLHKHPELEKLLREE 470
Query: 107 QSKEEEFKVQVKVLFEAKEEKFE 129
E+F+ + K L + +K E
Sbjct: 471 YRSLEDFRAKEKNLVKEDTQKVE 539
>AV770669
Length = 469
Score = 27.3 bits (59), Expect = 3.4
Identities = 14/40 (35%), Positives = 24/40 (60%), Gaps = 2/40 (5%)
Frame = +3
Query: 55 NILDGQVNEFESKKG--ELEGLLRDFESEKTNFERRQKEF 92
++ +V ES+K ++EG L F +KTN +R++ EF
Sbjct: 42 SVKQSKVKSLESRKRGIDMEGNLSSFLIKKTNLQRKRFEF 161
>TC12355 similar to UP|PR18_HUMAN (Q99633) Pre-mRNA splicing factor 18
(PRP18 homolog) (hPRP18), partial (8%)
Length = 563
Score = 27.3 bits (59), Expect = 3.4
Identities = 19/70 (27%), Positives = 38/70 (54%), Gaps = 11/70 (15%)
Frame = +3
Query: 24 RRLRSIKRDIEKCCEE----LENMETQVKE--FETEKNILDGQVNEF-----ESKKGELE 72
RRLR +K+ + E+ L+ ++ +K FE + ++ +GQ N+F E +K +
Sbjct: 321 RRLRFLKQPVTLFGEDDDARLDRLKHVLKTGLFEVDSDMTEGQTNDFLRDIAELRKRQKT 500
Query: 73 GLLRDFESEK 82
G++ D + +K
Sbjct: 501 GIMSDRKRQK 530
>TC12553 similar to GB|AAN31120.1|23506217|AY149966 At2g17220/T23A1.8
{Arabidopsis thaliana;}, partial (13%)
Length = 670
Score = 26.9 bits (58), Expect = 4.4
Identities = 14/42 (33%), Positives = 27/42 (63%), Gaps = 2/42 (4%)
Frame = +3
Query: 256 ISPNIKPCVRDEALKLARELKANMKE--NTENYLEVLGFLLI 295
+SPN +P + + KL+RELK +++ N ++ L +L L++
Sbjct: 234 LSPNHRPSMNEMLEKLSRELKQPVRDLWNPDSGLLILHLLML 359
>TC14500 UP|Q9S7B1 (Q9S7B1) Nodule INCEPTION protein, complete
Length = 3001
Score = 26.9 bits (58), Expect = 4.4
Identities = 28/118 (23%), Positives = 48/118 (39%), Gaps = 6/118 (5%)
Frame = +3
Query: 15 ECNGQQVEERRLRSIKRDIEKCCEEL-----ENMETQVKEFETEKNILDGQVNEFESKKG 69
+C+ + +++ L S+ +++ C L E+ T T K L+
Sbjct: 1302 DCHDSEEQKQLLNSLSMVVQQACRSLHVVLVEDEYTLPMPSHTSKEELE----------- 1448
Query: 70 ELEGLLRDFESEKTNFERRQKEFE-SKEKEFEIRVMEFQSKEEEFKVQVKVLFEAKEE 126
E E + + +K E E SKE + +ME Q K + V ++ L E KEE
Sbjct: 1449 EEEITITNNHEQKLFVSPSSHESECSKESSWIAHMMEAQQKGKGVSVSLEYLEEPKEE 1622
>TC13558 similar to GB|AAD33770.1|4928919|AF138744 zinc finger protein 2
{Arabidopsis thaliana;}, partial (8%)
Length = 553
Score = 26.9 bits (58), Expect = 4.4
Identities = 17/63 (26%), Positives = 39/63 (60%), Gaps = 4/63 (6%)
Frame = -1
Query: 70 ELEGLLRDFESEK-TNFERR--QKEFESKEKE-FEIRVMEFQSKEEEFKVQVKVLFEAKE 125
+++ ++RD E+ K NF R+ +K+ +++ + + R + +S++ + ++ +LFE E
Sbjct: 220 DVKMMIRDSEN*KGLNFPRKILKKKLKNRNQR*IQTR*EKIESEKNQM*EKITILFELDE 41
Query: 126 EKF 128
EKF
Sbjct: 40 EKF 32
>TC14415 similar to UP|O04237 (O04237) Transcription factor, partial (83%)
Length = 1666
Score = 26.9 bits (58), Expect = 4.4
Identities = 34/138 (24%), Positives = 60/138 (42%)
Frame = +2
Query: 51 ETEKNILDGQVNEFESKKGELEGLLRDFESEKTNFERRQKEFESKEKEFEIRVMEFQSKE 110
ETEKN+ D +K +E + + N E++EKE E + M + E
Sbjct: 824 ETEKNLSD--------EKPAVEDVADGNKESSAN--------ENEEKEPEDKEMTLEEYE 955
Query: 111 EEFKVQVKVLFEAKEEKFEVKMQQFENQVEDNLKSVKALELKENQIEVQIKDLKSKLNNF 170
+ + + K L K E +V +++FE S++ L K++ D+ +KL +
Sbjct: 956 KVLEEKRKALQAQKTEGRKVDIKEFE--------SMQPLSCKKDN-----NDIFAKLGS- 1093
Query: 171 GGQPKELELTEKQHDEEK 188
+ K E EK+ +K
Sbjct: 1094-DKDKRKEAFEKEEKAKK 1144
>AI967601
Length = 388
Score = 26.6 bits (57), Expect = 5.8
Identities = 17/81 (20%), Positives = 36/81 (43%), Gaps = 8/81 (9%)
Frame = +1
Query: 57 LDGQVNEFESKKGELEGLLRDFESEKTNFERRQKEFESKEKEFEIRVME--------FQS 108
+ GQ+ + L++ + E + + E+E K +E E + +E + +
Sbjct: 46 VSGQLKRVSESMNDRTRLIKKIKDEMNKLKSLEDEYERKLQE-EAKELEQLLSKKNIYSA 222
Query: 109 KEEEFKVQVKVLFEAKEEKFE 129
KEEE+ +++ L + FE
Sbjct: 223 KEEEYTKKIRELGPLTSDAFE 285
>TC18261 similar to UP|WSC2_YEAST (P53832) Cell wall integrity and stress
response component 2 precursor, partial (5%)
Length = 538
Score = 26.6 bits (57), Expect = 5.8
Identities = 20/70 (28%), Positives = 37/70 (52%), Gaps = 11/70 (15%)
Frame = +2
Query: 24 RRLRSIKRDIEKCCEE----LENMETQVKE--FETEKNILDGQVNEF-----ESKKGELE 72
RRLR +K+ I E+ L+ ++ +K FE + ++ +GQ N+F E +K +
Sbjct: 302 RRLRFLKQPITLFGEDDGARLDRLKHVLKTGLFEVDSDMTEGQTNDFLRDISELRKRQKT 481
Query: 73 GLLRDFESEK 82
G + D + +K
Sbjct: 482 GTMSDRKRQK 511
>BP034563
Length = 501
Score = 26.6 bits (57), Expect = 5.8
Identities = 13/46 (28%), Positives = 23/46 (49%)
Frame = -1
Query: 62 NEFESKKGELEGLLRDFESEKTNFERRQKEFESKEKEFEIRVMEFQ 107
++FE + +EGL D E Q++ E K++E + +E Q
Sbjct: 414 SQFEGLQKHMEGLTNDVERSNEMILMLQEKLEEKDEEIQRMKLEQQ 277
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.312 0.133 0.351
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,461,647
Number of Sequences: 28460
Number of extensions: 33264
Number of successful extensions: 210
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 201
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 208
length of query: 310
length of database: 4,897,600
effective HSP length: 90
effective length of query: 220
effective length of database: 2,336,200
effective search space: 513964000
effective search space used: 513964000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 55 (25.8 bits)
Medicago: description of AC149206.7