BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149205.5 + phase: 0 /pseudo
(738 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV767121 145 2e-35
AV780221 123 1e-28
TC13572 77 1e-15
BP041113 75 5e-14
AV780705 71 7e-13
TC18710 64 9e-11
AV414139 51 6e-07
BP060613 49 3e-06
TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%) 48 5e-06
BP056597 45 5e-05
TC12829 42 4e-04
AV412895 42 5e-04
BP078534 39 0.003
BP083121 33 0.005
AV426437 34 0.074
CB828232 34 0.074
BP085058 34 0.096
TC14123 similar to UP|Q93X25 (Q93X25) 2-Cys peroxiredoxin, parti... 34 0.096
TC8461 homologue to UP|AAQ08194 (AAQ08194) Eukaryotic translatio... 33 0.13
TC15408 similar to UP|Q9LIC2 (Q9LIC2) Multispanning membrane pro... 30 0.22
>AV767121
Length = 525
Score = 145 bits (367), Expect = 2e-35
Identities = 76/185 (41%), Positives = 105/185 (56%)
Frame = +2
Query: 401 LSGWQSCFLSFGGRLVLLKSVLTSLPVYALSFFKSPSGIISLIESLFNKFFWRGSEDNRK 460
LS W+ +SFGGR+ L++SVLT+LP++ LSFFK P G+ L F W GSE+ K
Sbjct: 2 LSKWKQKTVSFGGRIFLIQSVLTALPLFFLSFFKLPIGVGKSCVRLMRNFLWGGSENENK 181
Query: 461 ISWIAWSSVCLKKEFGGLGVRLLREFNLALMSKWCWRLLIDRGGFWYRVLVARYGEEARR 520
I+ + W+ VC KE GGLG++ L FN AL+ K WR L + W RV+ A+ +
Sbjct: 182 IA*VKWTDVCKPKELGGLGIKDLFTFNKALLGK*RWRYLTEPDSLWRRVIEAQPDHYS-- 355
Query: 521 LEVGGRSVSSWWKEVAKIKDGVGEDDEGWFVGKVSKLVGDGSNTLFWFDRWLGDVPFNRR 580
SSWW ++ + ED +GWF + KLVG+G T FW + WLG + R
Sbjct: 356 ------CGSSWWNDILSL---CPEDADGWFSSGLKKLVGEGDQTKFWSEDWLGSGLLSAR 508
Query: 581 FARLF 585
F RL+
Sbjct: 509 FRRLY 523
>AV780221
Length = 461
Score = 123 bits (308), Expect = 1e-28
Identities = 64/162 (39%), Positives = 96/162 (58%), Gaps = 3/162 (1%)
Frame = +3
Query: 415 LVLLKSVLTSLPVYALSFFKSPSG-IISLIESLFNKFFWRGSEDNRKISWIAWSSVCLKK 473
L L++SVL +LP++ L FFK P+G + + + + FF R E +KI+W+ WS+VC K
Sbjct: 3 LCLIRSVLIALPLFHLPFFKLPTGGFYTNVNN**DPFFGREVEGGKKIAWVKWSTVCRPK 182
Query: 474 EFGGLGVRLLREFNLALMSKWCWRLLIDRGGFWYRVLVARYGEEARRLEVGGRSVSSWWK 533
+ GGLGV+ L FN AL+ KW WR+L +R G WY+VL+ +Y +S SSWW
Sbjct: 183 DEGGLGVQNLGLFNKALLGKWRWRMLKERDGLWYKVLLIKYKNAI------PQSASSWWN 344
Query: 534 EVAK--IKDGVGEDDEGWFVGKVSKLVGDGSNTLFWFDRWLG 573
++ +DG G GW + + +G+G+ FW + WLG
Sbjct: 345 DLHSTCFEDGGG----GWMQRGLCRRIGEGTEVKFWNENWLG 458
>TC13572
Length = 534
Score = 77.0 bits (188), Expect(2) = 1e-15
Identities = 50/160 (31%), Positives = 78/160 (48%), Gaps = 6/160 (3%)
Frame = -3
Query: 506 WYRVLVARYGEEARRLEVGGRSVSSWWKEVAKIKDGVGEDDEGWFVGKVSKLVGDGSNTL 565
W RV++A+YG+ G ++SSWW++V G + GWF V K++ G++T
Sbjct: 478 WCRVVLAKYGDG------GCTNISSWWRDVQT----AGGGENGWFEEGVHKVLRSGNHTR 329
Query: 566 FWFDRWLGDVPFNRRFARLFDLTTNKMCTVADMYRLGWEEGGDAW--VWRRRLWAWEEEC 623
FW + W G ++ RL++L+ K ++ + GW +G W WRR L E +
Sbjct: 328 FWLENWTGVGILREKYYRLYNLSKLKWASIDECG--GWSQGVWNWDFRWRRPLAGRELDW 155
Query: 624 RSL--LDNVRLRL--NVLDRWQWDPDTIDVYTVRGVYQIL 659
LD VR+ L V D+W W P Y+V + L
Sbjct: 154 LQALSLDIVRVPLLAGVPDKWVWKPSEDGSYSVNSAFIFL 35
Score = 23.5 bits (49), Expect(2) = 1e-15
Identities = 8/12 (66%), Positives = 9/12 (74%)
Frame = -1
Query: 487 NLALMSKWCWRL 498
N AL+ KW WRL
Sbjct: 534 NKALLGKWLWRL 499
>BP041113
Length = 503
Score = 74.7 bits (182), Expect = 5e-14
Identities = 58/173 (33%), Positives = 73/173 (41%), Gaps = 10/173 (5%)
Frame = +1
Query: 566 FWFDRWLGDVPFNRRFARLFDLTTNKMCTVADMYRLGWEEGGDAW--VWRRRL----WAW 619
FW + W+G R+ RLF+L+ K C + + GW G W WRR L W
Sbjct: 1 FWTEDWIGMGTLRDRYYRLFNLSKEKRCCILECG--GWNHGVWTWKLSWRRALVGRELGW 174
Query: 620 EEECRSLLDNVRLRLNVLDRWQWDPDTIDVYTVRGVYQILTTSVSNTIVKTDEL---VWH 676
E L V L V D+W W P YTV Y L + T+ TD + +W
Sbjct: 175 LELMMKDLSGVCLSEGVEDKWLWLPG--GTYTVNSAYSFLQ---APTLADTDPIFATIWR 339
Query: 677 KQVPLN*VSIVAWRLLKDRLPTKTNLQRRDCLQATVNTCVSGC-GIAESASHL 728
P V AWR L RLPT NL +R + T C G ES +HL
Sbjct: 340 TVAPS--VKAFAWRCLLGRLPTYDNLIKRQVVVDPAMTVCKFCQGEVESVTHL 492
>AV780705
Length = 524
Score = 70.9 bits (172), Expect = 7e-13
Identities = 42/143 (29%), Positives = 73/143 (50%), Gaps = 3/143 (2%)
Frame = -2
Query: 375 YLGLSI--GGDPRKLAFWEPVVSTIKTRLSGWQSCFLSFGGRLVLLKSVLTSLPVYALSF 432
YLGL G + W + +K +L GW+ L+ G+ VL+K+++ ++P YA++
Sbjct: 484 YLGLPAIWGRNKSHSLVW--IEEKVKEKLEGWKETLLNQAGKEVLIKAIIQAIPSYAMTI 311
Query: 433 FKSPSGIISLIESLFNKFFWRG-SEDNRKISWIAWSSVCLKKEFGGLGVRLLREFNLALM 491
P + + +L F+W+ E + + + L KE GG+G R LR NLA +
Sbjct: 310 VHFPKTFCNSLYALVADFWWKSQGEGAVEYTGAVGTK*PLSKEKGGVGFRDLRTQNLAFL 131
Query: 492 SKWCWRLLIDRGGFWYRVLVARY 514
++ WR+L + W RV+ + Y
Sbjct: 130 ARQAWRVLTNPEALWVRVMKSLY 62
>TC18710
Length = 843
Score = 63.9 bits (154), Expect = 9e-11
Identities = 36/117 (30%), Positives = 61/117 (51%), Gaps = 3/117 (2%)
Frame = +2
Query: 169 VKDMQILDGILIANEV---VDEAKKTKKELLLFKVDFEKAYDSVD*GYLDAVMQKMAFPL 225
++ QI D +LI E ++ + + K+D KAYD V+ +L++ + F
Sbjct: 470 IQGRQIQDNLLIVQEAFHAINRPGALGRNHSIIKLDMNKAYDRVEWKFLESSLLAFGFST 649
Query: 226 LWRKWIKECISTTTASILVNGSPTDEFSLHRGLRQGDPLSPFLFMIAAEGLNVLMKS 282
W K I +S + + +NG + RGLRQGDP SP+LF+ E L++L+++
Sbjct: 650 NWVKMIMILVSGVSYNYKINGVVGPKLLPQRGLRQGDPFSPYLFLFTMEVLSLLIQN 820
>AV414139
Length = 309
Score = 51.2 bits (121), Expect = 6e-07
Identities = 33/96 (34%), Positives = 45/96 (46%), Gaps = 6/96 (6%)
Frame = -2
Query: 603 WEEGGDAWV--WRRRLWAWEEECRS----LLDNVRLRLNVLDRWQWDPDTIDVYTVRGVY 656
W G W WRR L E+ + L V L+ DRW+W DT D+YTVR Y
Sbjct: 290 WHNGVWEWEFRWRRELSVREQRQEADLIQALAGVCLQQGTEDRWKWTLDTEDIYTVRSAY 111
Query: 657 QILTTSVSNTIVKTDELVWHKQVPLN*VSIVAWRLL 692
+L ++++ + E VW P N + WRLL
Sbjct: 110 *LLLDPIADSDSEVYEKVWVGIAPSN-ANAFVWRLL 6
>BP060613
Length = 378
Score = 48.9 bits (115), Expect = 3e-06
Identities = 25/80 (31%), Positives = 39/80 (48%)
Frame = +1
Query: 452 WRGSEDNRKISWIAWSSVCLKKEFGGLGVRLLREFNLALMSKWCWRLLIDRGGFWYRVLV 511
W S+ R + W W + K+ GGLG + N AL++K WR+L + W ++L
Sbjct: 46 WWSSKGTRGVHWRKWDLLTELKDEGGLGFKDFEIQNQALLAKQAWRILHNPDALWVQILK 225
Query: 512 ARYGEEARRLEVGGRSVSSW 531
A Y L+ R+ +SW
Sbjct: 226 ALYFPHHDFLQTTKRTGASW 285
>TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%)
Length = 912
Score = 48.1 bits (113), Expect = 5e-06
Identities = 36/124 (29%), Positives = 62/124 (49%)
Frame = -3
Query: 302 VSHLQFADDTLLLGEKSWANVRALRAVLTLFAEMSGLKVNFNKSLLVGVNIGESWLLEAA 361
+SHL FAD+ ++ ++ + VL +F +SGL N KS V + + E+ +
Sbjct: 373 ISHLCFADNLMVFSNGYLESIAIINNVLHIFQHLSGLTPNPAKS-EVFILVKETTKNQIT 197
Query: 362 SVLNCKVGKILFLYLGLSIGGDPRKLAFWEPVVSTIKTRLSGWQSCFLSFGGRLVLLKSV 421
++L K GK+ LG+ K + + +V ++L W LS+ GRL L+ S+
Sbjct: 196 NMLGYKEGKLPVR*LGVPQLTTKLKASDCKVLVDRKPSKLQHWTGRSLSYAGRLQLINSI 17
Query: 422 LTSL 425
L S+
Sbjct: 16 LFSM 5
>BP056597
Length = 414
Score = 44.7 bits (104), Expect = 5e-05
Identities = 33/136 (24%), Positives = 58/136 (42%), Gaps = 11/136 (8%)
Frame = +3
Query: 581 FARLFDLTTNKMCTVADMYRLGWEEGGDAWVWRRRLWAWEEEC-----------RSLLDN 629
F LF ++ + C + +M W E + WVW ++W+ + ++ +
Sbjct: 9 FDSLFLISNQQNCKIVEMGH--WLE--NKWVWD---FSWKNPISGEDVLKLGVMKQIVSS 167
Query: 630 VRLRLNVLDRWQWDPDTIDVYTVRGVYQILTTSVSNTIVKTDELVWHKQVPLN*VSIVAW 689
L D W W + Y+V+ Y +L+ + + +W P N V++ W
Sbjct: 168 FSLINGKKDTWVWILEGDGQYSVKSAYDLLSGLDTTIGISVFSKLWKACAPSNAVAL-GW 344
Query: 690 RLLKDRLPTKTNLQRR 705
R+ DR+ TK NL RR
Sbjct: 345 RVFLDRIQTKDNLSRR 392
>TC12829
Length = 448
Score = 42.0 bits (97), Expect = 4e-04
Identities = 22/59 (37%), Positives = 32/59 (53%), Gaps = 2/59 (3%)
Frame = +3
Query: 408 FLSFGGRLVLLKSVLTSLPVYALSFFKSPSGIISLIESLFNKFF--WRGSEDNRKISWI 464
FL GR VL+KSV ++P Y +S F P I S IE + KF+ W + ++W+
Sbjct: 258 FLYRAGREVLIKSVTKAIPTYIMSCFALPDSICSQIEGMVGKFYLEW*CVSEEYSLAWM 434
Score = 28.5 bits (62), Expect = 4.0
Identities = 8/21 (38%), Positives = 10/21 (47%)
Frame = +1
Query: 450 FFWRGSEDNRKISWIAWSSVC 470
F W G R I W+ W +C
Sbjct: 385 FIWSGDVSRRSIHWLGWKKLC 447
>AV412895
Length = 405
Score = 41.6 bits (96), Expect = 5e-04
Identities = 21/60 (35%), Positives = 31/60 (51%), Gaps = 1/60 (1%)
Frame = +1
Query: 674 VWHKQVPLN*VSIVAWRLLKDRLPTKTNLQRRDCLQATVNTCVSGCGIA-ESASHLFLHC 732
+W PLN + WR+L R+ T+ NL +R + ++ CG+A ES SHL C
Sbjct: 82 IWMVPAPLN-IKAFVWRVLLGRIQTRDNLLKRQVIHNALDAICPLCGLAEESGSHLLFSC 258
>BP078534
Length = 410
Score = 38.9 bits (89), Expect = 0.003
Identities = 24/60 (40%), Positives = 29/60 (48%), Gaps = 4/60 (6%)
Frame = -3
Query: 567 WFDRWLGDVPFNRRFARLFDLTTNKMCTVADMYRLGWEEGGDAWVW----RRRLWAWEEE 622
W D W+ + F RLF L NK VAD +G GG +W W RR + WEEE
Sbjct: 216 WLDPWIRERALCDMFPRLFTLCENKGALVAD---VGSYHGG-SWHWNLSFRREFFQWEEE 49
>BP083121
Length = 527
Score = 33.1 bits (74), Expect(2) = 0.005
Identities = 15/27 (55%), Positives = 18/27 (66%)
Frame = +3
Query: 266 PFLFMIAAEGLNVLMKSVVENNLFKGY 292
P F+I AEGLN L+K V N FKG+
Sbjct: 312 PSFFLIVAEGLNGLLKQVATINKFKGF 392
Score = 23.9 bits (50), Expect(2) = 0.005
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = +2
Query: 251 EFSLHRGLRQGDPLSPFLF 269
EF + + L Q DP++ FLF
Sbjct: 266 EFKMWKSLHQVDPMAAFLF 322
>AV426437
Length = 303
Score = 34.3 bits (77), Expect = 0.074
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = +1
Query: 456 EDNRKISWIAWSSVCLKKEFGGLGVR 481
E+ RKI+WI W ++C K GLGV+
Sbjct: 214 ENQRKIAWINWETICKPKWLRGLGVK 291
>CB828232
Length = 532
Score = 34.3 bits (77), Expect = 0.074
Identities = 18/49 (36%), Positives = 30/49 (60%)
Frame = +1
Query: 404 WQSCFLSFGGRLVLLKSVLTSLPVYALSFFKSPSGIISLIESLFNKFFW 452
W++ LS G R+ K VL+SLP++ LSFFK + + + + ++F W
Sbjct: 388 WKTLCLS-GARVCCFKMVLSSLPLFFLSFFKIKC-VAKVCKQIQSRFLW 528
>BP085058
Length = 452
Score = 33.9 bits (76), Expect = 0.096
Identities = 22/80 (27%), Positives = 39/80 (48%)
Frame = +2
Query: 399 TRLSGWQSCFLSFGGRLVLLKSVLTSLPVYALSFFKSPSGIISLIESLFNKFFWRGSEDN 458
+RL+ ++ L+ RL L K VL+++ +Y + P I I ++ F W E+
Sbjct: 209 SRLAC*KAYLLNKPARLTLAKPVLSAISMYVMQLNWLPQSICDQICTVVRHFVW---ENV 379
Query: 459 RKISWIAWSSVCLKKEFGGL 478
I +AW ++ +K GL
Sbjct: 380 NGIPLVAWETIQSRKSMVGL 439
>TC14123 similar to UP|Q93X25 (Q93X25) 2-Cys peroxiredoxin, partial (89%)
Length = 1130
Score = 33.9 bits (76), Expect = 0.096
Identities = 11/21 (52%), Positives = 13/21 (61%)
Frame = -1
Query: 600 RLGWEEGGDAWVWRRRLWAWE 620
R+ W EG +W WR R W WE
Sbjct: 179 RVWWREG*GSWGWRERWWVWE 117
>TC8461 homologue to UP|AAQ08194 (AAQ08194) Eukaryotic translation
initiation factor 5A isoform IV, complete
Length = 796
Score = 33.5 bits (75), Expect = 0.13
Identities = 23/65 (35%), Positives = 32/65 (48%), Gaps = 11/65 (16%)
Frame = -3
Query: 494 WCWRLLID-RGGF---------WYRVLVARYGEEARR-LEVGGRSVSSWWKEVAKIKDGV 542
W W+L RGG WYR++ R+ RR G +WW+ +A+I DG
Sbjct: 230 WKWKLQQPCRGGS*C*CSRFCGWYRLVEGRFWRLRRRPWTRNGAPRQTWWRLLAEI-DGF 54
Query: 543 GEDDE 547
GED+E
Sbjct: 53 GEDEE 39
>TC15408 similar to UP|Q9LIC2 (Q9LIC2) Multispanning membrane protein-like,
partial (10%)
Length = 879
Score = 30.0 bits (66), Expect(2) = 0.22
Identities = 21/74 (28%), Positives = 31/74 (41%), Gaps = 8/74 (10%)
Frame = -1
Query: 610 WVW---RRRLWAWEE-----ECRSLLDNVRLRLNVLDRWQWDPDTIDVYTVRGVYQILTT 661
W W RRR + E + +LL++V L + D W W + YTV+ Y+ L
Sbjct: 768 WTWSFIRRRNPSTRENLLVADLCNLLESVPLSEDTNDSWSWTANPEGQYTVQTAYKFLRA 589
Query: 662 SVSNTIVKTDELVW 675
S + VW
Sbjct: 588 SSNEFTDPIFHFVW 547
Score = 21.2 bits (43), Expect(2) = 0.22
Identities = 7/18 (38%), Positives = 10/18 (54%)
Frame = -3
Query: 571 WLGDVPFNRRFARLFDLT 588
W G P N F +L+ L+
Sbjct: 874 WCGGTPLNISFCKLYHLS 821
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.335 0.146 0.498
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,342,252
Number of Sequences: 28460
Number of extensions: 240970
Number of successful extensions: 2175
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 2122
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2164
length of query: 738
length of database: 4,897,600
effective HSP length: 97
effective length of query: 641
effective length of database: 2,136,980
effective search space: 1369804180
effective search space used: 1369804180
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.6 bits)
S2: 59 (27.3 bits)
Medicago: description of AC149205.5