BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149197.4 - phase: 0
(204 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18883 68 1e-12
BP056414 60 2e-10
BP080173 48 1e-06
TC12547 45 9e-06
TC11323 38 0.001
BI417841 37 0.002
BP083970 37 0.002
TC16320 weakly similar to UP|Q84TE0 (Q84TE0) At5g51080, partial ... 34 0.020
BP069214 32 0.10
TC10933 similar to UP|Q9LNS0 (Q9LNS0) F1L3.4, partial (6%) 30 0.22
AV765095 29 0.50
AV779667 29 0.65
BP085925 29 0.65
BP062688 29 0.65
BP040706 28 1.1
TC13772 28 1.5
BI417620 27 1.9
TC15471 27 2.5
TC15715 similar to PIR|S33804|S33804 male sterility protein 2 - ... 27 3.2
TC7929 similar to UP|GGH_ARATH (O65355) Gamma-glutamyl hydrolase... 27 3.2
>TC18883
Length = 821
Score = 67.8 bits (164), Expect = 1e-12
Identities = 37/122 (30%), Positives = 62/122 (50%), Gaps = 1/122 (0%)
Frame = +1
Query: 59 GKAACGGIFRNRLGQFLGCFAEGLSYGNSLFAELCGAMRSIEFAQSKSWNNFWLETDSML 118
G+ GGIFRN + LG F++ + G + AE+ ++ ++ N +E+DS L
Sbjct: 1 GRCGIGGIFRNSDNEILGFFSKHVGEGFAFEAEVWAIFEALTICVNRQLRNLTIESDSSL 180
Query: 119 VVQAFKNSAIVPWQVRNRWNNCQIIISNMNFLA-SHIYREGNSCADVLANIGLTLDTYVY 177
V N + PW++ N+ N + ++ +N L+ HI+RE N D LA G +Y
Sbjct: 181 AVGWVNNRSXRPWKLLNKLNQIDLWVAEVNCLSVKHIFREANGEVDKLAKRGADHPRDLY 360
Query: 178 YY 179
Y+
Sbjct: 361 YF 366
>BP056414
Length = 606
Score = 60.5 bits (145), Expect = 2e-10
Identities = 44/149 (29%), Positives = 70/149 (46%), Gaps = 6/149 (4%)
Frame = -3
Query: 40 WSPPIHSWIKGNCVGAFASG-KAACGGIFRNRLGQFLGCFAEGLS---YGNSLFAELCGA 95
W+ P W K N GA + +A+CGG+ R+ G + FA L + + F EL
Sbjct: 562 WTRPPEGWFKVNVDGALSGDLQASCGGVVRDAAGSWSKGFARSLGVLRWHXAFFVELMAV 383
Query: 96 MRSIEFAQSKSWNNFWLETDSMLVVQAFKN-SAIVPWQVRN-RWNNCQIIISNMNFLASH 153
+++F S +E+DS VV+ +N +A P Q W+ Q I + + + +
Sbjct: 382 QTAVDFIMSWDIPQVIIESDSQQVVELLQNFNASSPHQYGEIAWDIQQKISVHGSIVITS 203
Query: 154 IYREGNSCADVLANIGLTLDTYVYYYSLP 182
RE N AD LA +GL+L + + P
Sbjct: 202 TPREANFLADYLAKVGLSLPFGTHSFDAP 116
>BP080173
Length = 382
Score = 47.8 bits (112), Expect = 1e-06
Identities = 30/85 (35%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = -2
Query: 38 VLWSPPIHSWIKGNCVGAFA--SGKAACGGIFRNRLGQFLGCFAEGLSYGNSLFAELCGA 95
V W P+ WIK N G+FA S AACGG+ + G F+ + L AEL G
Sbjct: 351 VRWPVPMEGWIKINIDGSFAPSSNCAACGGVMWDHEGTFITGASVRLGCYFINHAELWGI 172
Query: 96 MRSIEFAQSKSWNNFWLETDSMLVV 120
+ AQ++ + +E+DS VV
Sbjct: 171 FHGAKIAQARGFKKILVESDSSFVV 97
>TC12547
Length = 924
Score = 45.1 bits (105), Expect = 9e-06
Identities = 35/125 (28%), Positives = 58/125 (46%), Gaps = 4/125 (3%)
Frame = +1
Query: 59 GKAACGGIFRNRLGQFLGCFAEGLSYGNSLFAELCGA---MRSIEFAQSKSWNNFWLETD 115
G + G+ RNR G+ LGCF+ + G + +R S + N +E+D
Sbjct: 34 GDSGVAGVLRNREGKILGCFS--IPMGRKWLSRQK*KPF*VR*RFVRSSINVKNLTIESD 207
Query: 116 SMLVVQAFKNSAIVPWQVRNRWNNCQIIISNMNFLA-SHIYREGNSCADVLANIGLTLDT 174
S +V+ NS PW++ N N I+ +N L HI+RE N + A +G T +
Sbjct: 208 STMVMV*ANNSVPRPWKLVNELNLIDILRYEVNCLGIFHIFREANFLLMLWAELGCTCEN 387
Query: 175 YVYYY 179
++ +
Sbjct: 388 PLWAF 402
>TC11323
Length = 657
Score = 38.1 bits (87), Expect = 0.001
Identities = 25/119 (21%), Positives = 51/119 (42%), Gaps = 2/119 (1%)
Frame = +2
Query: 40 WSPPIHSWIKGNCVGAFAS--GKAACGGIFRNRLGQFLGCFAEGLSYGNSLFAELCGAMR 97
W P+H K N G+ G A GG+ R+ F+ F+ L+ + LC +
Sbjct: 5 WLKPVHGRFKLNFDGSSLGNRGNAGGGGLLRDGSSNFIFGFSIFLAVAQIMKLSLCAILE 184
Query: 98 SIEFAQSKSWNNFWLETDSMLVVQAFKNSAIVPWQVRNRWNNCQIIISNMNFLASHIYR 156
+ + ++ +E DS +VV ++ + W + + + ++ ++ HI+R
Sbjct: 185 GLLVCKPLGYDGIGIECDSNIVVSWIRSRSCQLWYLWDF*GPLKGLLDVKDYSIHHIFR 361
>BI417841
Length = 617
Score = 37.4 bits (85), Expect = 0.002
Identities = 33/120 (27%), Positives = 56/120 (46%), Gaps = 6/120 (5%)
Frame = +1
Query: 59 GKAACGGIFRNRLGQFLGCFAEGLSYGNSLFAELCGAMRSIEFAQSKSWNNFWLETDSML 118
G A G + R G + EG+ + AE G + ++ A + + + ++ DS L
Sbjct: 175 GSAGAGAVLRAEDGSKV-YLREGVGNQTNNQAEYRGLILGLKHAHEQGYQHINVKGDSQL 351
Query: 119 VVQAFKNSAIVPWQVRNR-----WNNCQIIISNM-NFLASHIYREGNSCADVLANIGLTL 172
V + + S W+ RN N + + S +F +H+ R+ NS ADV AN+G+ L
Sbjct: 352 VCKQVEGS----WKARNPNIASLCNEAKELKSKFQSFDINHVPRQYNSEADVQANLGVNL 519
>BP083970
Length = 375
Score = 37.0 bits (84), Expect = 0.002
Identities = 25/73 (34%), Positives = 36/73 (49%), Gaps = 1/73 (1%)
Frame = -3
Query: 109 NFWLETDSMLVVQAFKNSAIVPWQVRNRWNNCQIIISNMNFL-ASHIYREGNSCADVLAN 167
N + +DS L V N + PW++ + N + +N L SHIYRE NS AD LA
Sbjct: 364 NISI*SDSSLAVGWINNCSNRPWKLLSMLNPIDHWLVEVNCLKVSHIYREANSEADKLAK 185
Query: 168 IGLTLDTYVYYYS 180
G + Y++
Sbjct: 184 RGAIHSKDLVYFN 146
>TC16320 weakly similar to UP|Q84TE0 (Q84TE0) At5g51080, partial (11%)
Length = 632
Score = 33.9 bits (76), Expect = 0.020
Identities = 25/87 (28%), Positives = 44/87 (49%), Gaps = 8/87 (9%)
Frame = +2
Query: 94 GAMRSIEFAQSKSWNNFWLETDSMLVVQAFKNSAIVPWQVRNRWNNCQIIISN------- 146
G + ++ A + + + ++ DSMLV + W+++N+ N + S
Sbjct: 8 GLILGLKHAIKEGYKHIQVKGDSMLVCNQVQGL----WKIKNQ--NIASLCSEAKELKNK 169
Query: 147 -MNFLASHIYREGNSCADVLANIGLTL 172
++F +HI RE NS ADV AN G++L
Sbjct: 170 FLSFKINHIPREYNSEADVQANFGISL 250
>BP069214
Length = 545
Score = 31.6 bits (70), Expect = 0.10
Identities = 14/37 (37%), Positives = 19/37 (50%)
Frame = -2
Query: 137 WNNCQIIISNMNFLASHIYREGNSCADVLANIGLTLD 173
W+ ++ +H+ REGNSCAD LA G D
Sbjct: 253 WSIKDLLSREWEVSMTHMLREGNSCADFLAKYGAMQD 143
>TC10933 similar to UP|Q9LNS0 (Q9LNS0) F1L3.4, partial (6%)
Length = 596
Score = 30.4 bits (67), Expect = 0.22
Identities = 12/29 (41%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Frame = +2
Query: 40 WSPPIHSWIKGNCVGAFASGKA--ACGGI 66
W+PP W+K N G+F G + CGG+
Sbjct: 107 WTPPSVGWVKLNTDGSFTRGSSLMGCGGL 193
>AV765095
Length = 378
Score = 29.3 bits (64), Expect = 0.50
Identities = 13/34 (38%), Positives = 22/34 (64%), Gaps = 1/34 (2%)
Frame = -3
Query: 130 PWQVRNRWNNCQIIISNMNFLAS-HIYREGNSCA 162
PW++ N N +++S ++ + HI+REGNS A
Sbjct: 331 PWKLLNDLNLIDLLLSELDCIEIFHIFREGNSMA 230
>AV779667
Length = 455
Score = 28.9 bits (63), Expect = 0.65
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = -3
Query: 151 ASHIYREGNSCADVLA 166
+SH+ REGNSCAD LA
Sbjct: 174 SSHMGREGNSCADYLA 127
>BP085925
Length = 334
Score = 28.9 bits (63), Expect = 0.65
Identities = 14/47 (29%), Positives = 22/47 (46%)
Frame = -2
Query: 137 WNNCQIIISNMNFLASHIYREGNSCADVLANIGLTLDTYVYYYSLPL 183
W+ ++ + + + +H REGN+CAD L G D PL
Sbjct: 288 WDIQDLMSRSWHVVLNHTLREGNACADFLTKHGAGQDEAFVVIDNPL 148
>BP062688
Length = 536
Score = 28.9 bits (63), Expect = 0.65
Identities = 10/29 (34%), Positives = 19/29 (65%)
Frame = -1
Query: 142 IIISNMNFLASHIYREGNSCADVLANIGL 170
++ + N +H+ R+GN+ AD LAN+ +
Sbjct: 242 VLARSWNVTINHVTRDGNAVADALANLAI 156
>BP040706
Length = 506
Score = 28.1 bits (61), Expect = 1.1
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = +2
Query: 152 SHIYREGNSCADVLANIGLTLDTYVYYYSLPLQV 185
SH+YRE N+ AD LA G + + Y+ L V
Sbjct: 212 SHVYREANTEADKLAKRGAAFVSDLVYFHSSLAV 313
>TC13772
Length = 409
Score = 27.7 bits (60), Expect = 1.5
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +1
Query: 150 LASHIYREGNSCADVLANIG 169
+ SHIYRE N AD LA G
Sbjct: 58 VTSHIYREANESADSLAKAG 117
>BI417620
Length = 406
Score = 27.3 bits (59), Expect = 1.9
Identities = 16/41 (39%), Positives = 20/41 (48%), Gaps = 1/41 (2%)
Frame = +1
Query: 130 PWQVRNRWNNCQIIISNMNFLA-SHIYREGNSCADVLANIG 169
PW + N N + + SHI REGN+ AD LA G
Sbjct: 1 PWNLTNELNMIDYLFPLVACTGVSHILREGNAEADELAKEG 123
>TC15471
Length = 642
Score = 26.9 bits (58), Expect = 2.5
Identities = 12/22 (54%), Positives = 15/22 (67%), Gaps = 1/22 (4%)
Frame = +2
Query: 134 RNRWNNCQIIISNM-NFLASHI 154
+N WN+C II NM N LA H+
Sbjct: 449 KNIWNSCMTIIFNMDNMLALHL 514
>TC15715 similar to PIR|S33804|S33804 male sterility protein 2 - Arabidopsis
thaliana {Arabidopsis thaliana;}, partial (13%)
Length = 666
Score = 26.6 bits (57), Expect = 3.2
Identities = 16/44 (36%), Positives = 27/44 (61%), Gaps = 2/44 (4%)
Frame = -3
Query: 140 CQIIISNMNFLASHIYREGNSCADVLANIGLTL--DTYVYYYSL 181
C II S ++++ + +G +C+ NIGL + + YV+YYSL
Sbjct: 634 CFIISSKLHYMVN----KGYTCS--YCNIGLQVKKENYVFYYSL 521
>TC7929 similar to UP|GGH_ARATH (O65355) Gamma-glutamyl hydrolase precursor
(Gamma-Glu-X carboxypeptidase) (Conjugase) (GH) , partial
(83%)
Length = 1299
Score = 26.6 bits (57), Expect = 3.2
Identities = 20/76 (26%), Positives = 38/76 (49%), Gaps = 14/76 (18%)
Frame = -1
Query: 80 EGLSYGNS--LFAELCGAMRSIEFAQSKSWNN-----------FWLETDSMLVVQAFKNS 126
+G S+ N+ LF+ LC A+ ++ + +N +WL + + +VQ +K
Sbjct: 1227 KGHSFWNNGILFSFLCIAITTMIITHTTLHHNKHHTYMHVCYYYWLISPAFRLVQIYKLH 1048
Query: 127 AIVPWQVRNRWN-NCQ 141
I+ RN+W+ NC+
Sbjct: 1047 HILSQPSRNKWDCNCK 1000
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.135 0.433
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,537,963
Number of Sequences: 28460
Number of extensions: 69072
Number of successful extensions: 348
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 345
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 346
length of query: 204
length of database: 4,897,600
effective HSP length: 86
effective length of query: 118
effective length of database: 2,450,040
effective search space: 289104720
effective search space used: 289104720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 53 (25.0 bits)
Medicago: description of AC149197.4