Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC149135.13 - phase: 0 
         (316 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC12895 homologue to UP|Q8LAA2 (Q8LAA2) Cell division-like prote...   210  3e-55
AV774825                                                              134  2e-32
AV776603                                                               27  4.5
TC9313 homologue to UP|O82529 (O82529) Ribosomal protein L27a, p...    26  7.7

>TC12895 homologue to UP|Q8LAA2 (Q8LAA2) Cell division-like protein, partial
           (34%)
          Length = 433

 Score =  210 bits (534), Expect = 3e-55
 Identities = 104/105 (99%), Positives = 105/105 (99%)
 Frame = +2

Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGV+RVVDLCAAPGSWSQVLS
Sbjct: 119 MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVRRVVDLCAAPGSWSQVLS 298

Query: 61  RKLYLPAKLAPDAKDENLPLIVAIDLQPMAPIEGVIQVQGDITNA 105
           RKLYLPAKLAPDAKDENLPLIVAIDLQPMAPIEGVIQVQGDITNA
Sbjct: 299 RKLYLPAKLAPDAKDENLPLIVAIDLQPMAPIEGVIQVQGDITNA 433


>AV774825 
          Length = 461

 Score =  134 bits (338), Expect = 2e-32
 Identities = 61/70 (87%), Positives = 66/70 (94%)
 Frame = -3

Query: 233 VDDTDCVSGWLEGPNKVYIPFLACGDLTGYDSDRSYPLPKVAGGTYQSLDPVQPPIAPPY 292
           ++DTD  SGWLEGPNKVYIPFLACGDL+GYD DRSYPLPK+ GGTYQSLDPVQPPIAPPY
Sbjct: 459 LEDTDSCSGWLEGPNKVYIPFLACGDLSGYDFDRSYPLPKLDGGTYQSLDPVQPPIAPPY 280

Query: 293 KRALELKKAS 302
           +RALELKKAS
Sbjct: 279 QRALELKKAS 250



 Score = 30.8 bits (68), Expect = 0.31
 Identities = 18/31 (58%), Positives = 19/31 (61%), Gaps = 1/31 (3%)
 Frame = -1

Query: 287 PIAPPYKRALELKKASPQ-GFRELENLSLDS 316
           P+ P   R L   K  P  GFRELENLSLDS
Sbjct: 299 PLLPLPTRGLSS*KKPPHPGFRELENLSLDS 207



 Score = 29.6 bits (65), Expect = 0.70
 Identities = 18/50 (36%), Positives = 24/50 (48%), Gaps = 2/50 (4%)
 Frame = +2

Query: 241 GWLEGPNKVYIPFLACGDLTGYDSDRSYPL--PKVAGGTYQSLDPVQPPI 288
           G   G    Y+P  + G  +GYD  +SYPL  P+   G Y  L P   P+
Sbjct: 296 GGCTGSKL*YVPPSSLG--SGYDLSKSYPLRSPQARNGIYTLLGPSNHPL 439


>AV776603 
          Length = 359

 Score = 26.9 bits (58), Expect = 4.5
 Identities = 11/21 (52%), Positives = 13/21 (61%)
 Frame = +2

Query: 228 GSPSGVDDTDCVSGWLEGPNK 248
           GS SG  +TD  +GW   PNK
Sbjct: 218 GSKSGTSNTDVGAGWNNKPNK 280


>TC9313 homologue to UP|O82529 (O82529) Ribosomal protein L27a, partial
          (94%)
          Length = 624

 Score = 26.2 bits (56), Expect = 7.7
 Identities = 10/19 (52%), Positives = 14/19 (73%)
 Frame = +2

Query: 6  RDKRDIYYRKAKEEGWRAR 24
          R++R   YRKA+E  WR+R
Sbjct: 80 RERRSRPYRKAQEASWRSR 136


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.319    0.139    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,427,834
Number of Sequences: 28460
Number of extensions: 78801
Number of successful extensions: 374
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 372
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 374
length of query: 316
length of database: 4,897,600
effective HSP length: 90
effective length of query: 226
effective length of database: 2,336,200
effective search space: 527981200
effective search space used: 527981200
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)


Medicago: description of AC149135.13