BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149131.7 + phase: 0
(111 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9284 weakly similar to UP|Q9LPY0 (Q9LPY0) T23J18.22, partial (... 42 2e-05
TC16410 similar to UP|Q9XHL6 (Q9XHL6) Sucrose transport protein ... 34 0.004
TC9116 similar to UP|Q9ZW85 (Q9ZW85) 3-isopropylmalate dehydrata... 33 0.007
TC19923 33 0.007
AV777934 33 0.007
BP072816 33 0.007
AV410591 33 0.007
TC18665 similar to UP|O23061 (O23061) BAC IG005I10, partial (65%) 33 0.009
TC11009 similar to GB|AAP21377.1|30102918|BT006569 At1g47970 {Ar... 33 0.009
TC7994 similar to UP|Q8RU73 (Q8RU73) Ribose-5-phosphate isomeras... 33 0.009
TC11455 similar to UP|Q9ZUS8 (Q9ZUS8) At2g37380 protein, partial... 33 0.009
TC11441 similar to PIR|S54157|S54157 extensin-like protein - cow... 32 0.012
TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%) 32 0.015
TC15802 similar to UP|Q9XIV1 (Q9XIV1) mRNA expressed in cucumber... 32 0.015
TC14933 homologue to UP|TCMO_GLYEC (Q96423) Trans-cinnamate 4-mo... 32 0.015
TC15016 weakly similar to UP|Q8L685 (Q8L685) Pherophorin-dz1 pro... 32 0.020
TC10688 similar to UP|AAR10812 (AAR10812) Superoxide dismutase, ... 32 0.020
TC13802 similar to UP|Q9M1T1 (Q9M1T1) Sugar-phosphate isomerase-... 31 0.026
TC9037 similar to GB|AAG24276.1|10880493|AF195889 fasciclin-like... 31 0.026
TC20065 similar to UP|AAR20725 (AAR20725) At5g18690, partial (21%) 31 0.026
>TC9284 weakly similar to UP|Q9LPY0 (Q9LPY0) T23J18.22, partial (33%)
Length = 898
Score = 41.6 bits (96), Expect = 2e-05
Identities = 25/66 (37%), Positives = 37/66 (55%)
Frame = +1
Query: 24 DNSHAVSSTTTEPTISASPGVLPYVTSPDISSFFPTPMSSSEAPYEAEASAPAPAPSSGE 83
D + A S++TT+P+ + SP P+ +P SS TP+SS+ P A S +PSS
Sbjct: 193 DPNPASSASTTKPSAATSPPSKPHAPTPSSSS--STPLSSTTNPNSASPSFATSSPSSPP 366
Query: 84 KSSSST 89
SS +T
Sbjct: 367 PSSPTT 384
>TC16410 similar to UP|Q9XHL6 (Q9XHL6) Sucrose transport protein SUT1,
partial (27%)
Length = 502
Score = 33.9 bits (76), Expect = 0.004
Identities = 25/68 (36%), Positives = 31/68 (44%), Gaps = 9/68 (13%)
Frame = +3
Query: 30 SSTTTEPTISASPGVLPYVTSPDISSFFPTPMSSSEAPYEAEAS---------APAPAPS 80
S TT + AS +P +SP S P+P SSS P + APAP+PS
Sbjct: 303 SVTTATAALHASAAAVP--SSPPAHSPSPSPSSSSATPPTSATP*ATGLTRRFAPAPSPS 476
Query: 81 SGEKSSSS 88
S S SS
Sbjct: 477 SSSDSGSS 500
>TC9116 similar to UP|Q9ZW85 (Q9ZW85) 3-isopropylmalate dehydratase, small
subunit, partial (25%)
Length = 426
Score = 33.1 bits (74), Expect = 0.007
Identities = 21/56 (37%), Positives = 28/56 (49%), Gaps = 1/56 (1%)
Frame = +1
Query: 30 SSTTTEPTISASPGVLPYVTSPDISSFFPTPMSS-SEAPYEAEASAPAPAPSSGEK 84
S+TT+ PT S+ P + P S PTP S+ S AP + AS P P S +
Sbjct: 256 SATTSTPTRSSPPSISP--------SSLPTPTSTRSSAPTPSSASPPPTPPDSSNR 399
>TC19923
Length = 700
Score = 33.1 bits (74), Expect = 0.007
Identities = 24/75 (32%), Positives = 32/75 (42%), Gaps = 11/75 (14%)
Frame = +1
Query: 28 AVSSTTTEPTISASPGVLPYVTSPDISSFFPTPMSSSEAPYEA------EASAPAPAPS- 80
++ T EPT+ P VL PD+ + FP P S P A A P PA S
Sbjct: 181 SIQVPTMEPTLPQGPAVLVVPFRPDLQTAFPVPNISELMPKPAPVVSQPHARYPTPADSW 360
Query: 81 ----SGEKSSSSTRL 91
GE+ +T+L
Sbjct: 361 PSQLLGEQPHFTTKL 405
>AV777934
Length = 265
Score = 33.1 bits (74), Expect = 0.007
Identities = 21/62 (33%), Positives = 33/62 (52%), Gaps = 2/62 (3%)
Frame = +1
Query: 29 VSSTTTEPTISASPGVLPYVTSPDISSFFPTPMSSSEA--PYEAEASAPAPAPSSGEKSS 86
+S T T P+ A P P +T+ +SF P P SS++ P +P+P+PS+ S
Sbjct: 64 LSQTLTPPSAMAPP---PPMTAITATSFPPPPDSSTKTLPPSHTTTGSPSPSPSASTPPS 234
Query: 87 SS 88
+S
Sbjct: 235 TS 240
>BP072816
Length = 492
Score = 33.1 bits (74), Expect = 0.007
Identities = 18/54 (33%), Positives = 31/54 (57%)
Frame = +1
Query: 25 NSHAVSSTTTEPTISASPGVLPYVTSPDISSFFPTPMSSSEAPYEAEASAPAPA 78
N+ + ST+++PT SA+P +++P + P+ SS+ +P A SAP A
Sbjct: 247 NTSSKPSTSSKPTTSAAPPASSLLSTPPKPAGPPSNPSSTNSPAPASTSAPHAA 408
Score = 27.3 bits (59), Expect = 0.38
Identities = 20/64 (31%), Positives = 34/64 (52%), Gaps = 4/64 (6%)
Frame = +1
Query: 30 SSTTTEPTISASPGVLPYVTSPDISSFFPTPMSSSEAPYEAEASAP----APAPSSGEKS 85
S+T P +S P P +S SS P+P ++ AP + +SAP AP PS+ +
Sbjct: 67 SNTHHNPPLSHQPCHSPSSSSSS-SSPLPSPPTTLHAPSTSPSSAPSATAAPPPSTPTPA 243
Query: 86 SSST 89
++++
Sbjct: 244 ANTS 255
Score = 26.6 bits (57), Expect = 0.65
Identities = 16/59 (27%), Positives = 31/59 (52%), Gaps = 1/59 (1%)
Frame = +1
Query: 30 SSTTTEPTISASPGVLPYVTSPDISSFFPTPMSSSEAPYE-AEASAPAPAPSSGEKSSS 87
++T+++P+ S+ P +P SS TP + P + ++PAPA +S ++S
Sbjct: 244 ANTSSKPSTSSKPTT---SAAPPASSLLSTPPKPAGPPSNPSSTNSPAPASTSAPHAAS 411
>AV410591
Length = 429
Score = 33.1 bits (74), Expect = 0.007
Identities = 22/68 (32%), Positives = 32/68 (46%), Gaps = 9/68 (13%)
Frame = +2
Query: 31 STTTEPTISASPGV---------LPYVTSPDISSFFPTPMSSSEAPYEAEASAPAPAPSS 81
S++ PT+S S G P + P SS P P +S+ + AS P P P+S
Sbjct: 77 SSSLYPTLSTSFGAPTPASAKP*SPPASPPPPSSLPPPPSNSTTSSPSKPASLPTPTPAS 256
Query: 82 GEKSSSST 89
SSS++
Sbjct: 257 SSTSSSTS 280
>TC18665 similar to UP|O23061 (O23061) BAC IG005I10, partial (65%)
Length = 451
Score = 32.7 bits (73), Expect = 0.009
Identities = 19/59 (32%), Positives = 34/59 (57%)
Frame = +2
Query: 31 STTTEPTISASPGVLPYVTSPDISSFFPTPMSSSEAPYEAEASAPAPAPSSGEKSSSST 89
++ + P+ + V +SP SS +P+SSS P E+ +S+ +P+P S +SSS+
Sbjct: 68 TSCSSPSKPEASSVASSSSSPSPSS--SSPISSSRKPSESRSSSSSPSPDSKSATSSSS 238
Score = 24.6 bits (52), Expect = 2.5
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = -1
Query: 55 SFFPTPMSSSEAPYEAEASAPAPAPS 80
S FPTP E+P + +P+ +PS
Sbjct: 403 SQFPTPYPRRESPSQTHPPSPSGSPS 326
>TC11009 similar to GB|AAP21377.1|30102918|BT006569 At1g47970 {Arabidopsis
thaliana;}, partial (44%)
Length = 700
Score = 32.7 bits (73), Expect = 0.009
Identities = 22/67 (32%), Positives = 37/67 (54%)
Frame = -2
Query: 25 NSHAVSSTTTEPTISASPGVLPYVTSPDISSFFPTPMSSSEAPYEAEASAPAPAPSSGEK 84
+S + SS+++ + S SP ++P SS + SSS P + +S+ +P+ SS
Sbjct: 219 SSSSSSSSSSSSSSSGSPPPPRPPSTPPSSSSSSSSSSSSPPPGASSSSSSSPSSSSPSS 40
Query: 85 SSSSTRL 91
SSSS+ L
Sbjct: 39 SSSSSPL 19
Score = 26.6 bits (57), Expect = 0.65
Identities = 18/60 (30%), Positives = 28/60 (46%)
Frame = -2
Query: 30 SSTTTEPTISASPGVLPYVTSPDISSFFPTPMSSSEAPYEAEASAPAPAPSSGEKSSSST 89
SS++ T+ + +P +S SS + SSS + P+ PSS SSSS+
Sbjct: 285 SSSSAAATVRRTRYSVPKSSSSSSSSSSSSSSSSSSSGSPPPPRPPSTPPSSSSSSSSSS 106
>TC7994 similar to UP|Q8RU73 (Q8RU73) Ribose-5-phosphate isomerase
precursor , partial (81%)
Length = 1355
Score = 32.7 bits (73), Expect = 0.009
Identities = 20/47 (42%), Positives = 25/47 (52%)
Frame = +2
Query: 42 PGVLPYVTSPDISSFFPTPMSSSEAPYEAEASAPAPAPSSGEKSSSS 88
P P TS D PT S++ +P + ASAPAP P S SS+S
Sbjct: 386 PSPSPKTTSRDSP---PTRPSTTSSPAWSSASAPAPPPPSSSPSSAS 517
>TC11455 similar to UP|Q9ZUS8 (Q9ZUS8) At2g37380 protein, partial (10%)
Length = 575
Score = 32.7 bits (73), Expect = 0.009
Identities = 28/83 (33%), Positives = 36/83 (42%), Gaps = 17/83 (20%)
Frame = +1
Query: 26 SHAVSSTTTEPTISASP-----------------GVLPYVTSPDISSFFPTPMSSSEAPY 68
SH+ SS+ E TIS SP +LP SP IS +SSS
Sbjct: 133 SHSSSSSDFEFTISISPRKSSTALCPADELFYKGQLLPLHLSPRISMVRTLLLSSSSTSS 312
Query: 69 EAEASAPAPAPSSGEKSSSSTRL 91
A +S AP S+G SS ++ L
Sbjct: 313 AASSSTTAPRDSTGSSSSFNSDL 381
Score = 25.4 bits (54), Expect = 1.4
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = +2
Query: 62 SSSEAPYEAEASAPAPAPSSGEKSSS 87
S++ +P A+ PAPAPS SSS
Sbjct: 368 STATSPSSPTATPPAPAPSQRTTSSS 445
>TC11441 similar to PIR|S54157|S54157 extensin-like protein - cowpea
(fragment) {Vigna unguiculata;} , partial (6%)
Length = 541
Score = 32.3 bits (72), Expect = 0.012
Identities = 25/68 (36%), Positives = 33/68 (47%)
Frame = +2
Query: 19 VIILLDNSHAVSSTTTEPTISASPGVLPYVTSPDISSFFPTPMSSSEAPYEAEASAPAPA 78
VI+L D S++ SS T + T +ASP P+P SSS +P P+PA
Sbjct: 2 VIVLADRSNSNSSNTNQTT-TASP---------------PSPQSSSSSP-------PSPA 112
Query: 79 PSSGEKSS 86
PS E S
Sbjct: 113PSKQEGQS 136
>TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%)
Length = 1008
Score = 32.0 bits (71), Expect = 0.015
Identities = 21/60 (35%), Positives = 33/60 (55%), Gaps = 2/60 (3%)
Frame = +1
Query: 25 NSHAVSSTTTEPTISASP--GVLPYVTSPDISSFFPTPMSSSEAPYEAEASAPAPAPSSG 82
+S ++SS++T + S+SP +S SS + +P SSS + + APAP P SG
Sbjct: 298 SSSSLSSSSTPSSSSSSPPSSSSSSSSSSSSSSSWSSPSSSSSSSPFSSGPAPAPGPPSG 477
Score = 27.7 bits (60), Expect = 0.29
Identities = 15/40 (37%), Positives = 22/40 (54%)
Frame = +1
Query: 50 SPDISSFFPTPMSSSEAPYEAEASAPAPAPSSGEKSSSST 89
S SS + +SSS P + +S P+ + SS SSSS+
Sbjct: 277 SSSSSSSSSSSLSSSSTPSSSSSSPPSSSSSSSSSSSSSS 396
>TC15802 similar to UP|Q9XIV1 (Q9XIV1) mRNA expressed in cucumber hypocotyls
(Arabinogalactan protein), partial (26%)
Length = 795
Score = 32.0 bits (71), Expect = 0.015
Identities = 19/69 (27%), Positives = 35/69 (50%)
Frame = +2
Query: 13 LAFVFVVIILLDNSHAVSSTTTEPTISASPGVLPYVTSPDISSFFPTPMSSSEAPYEAEA 72
LA + +V+ + ++ TT P A+P V++P P P +++ A + A
Sbjct: 68 LALICIVVAGVGGQSPTAAPTTSP---ATPSATTPVSTPVAPVASPKPAATTPAASPSTA 238
Query: 73 SAPAPAPSS 81
+APAPA ++
Sbjct: 239 TAPAPATTT 265
Score = 28.9 bits (63), Expect = 0.13
Identities = 16/52 (30%), Positives = 22/52 (41%)
Frame = +2
Query: 36 PTISASPGVLPYVTSPDISSFFPTPMSSSEAPYEAEASAPAPAPSSGEKSSS 87
P S P +P + P S P P + A PAPAPS +K ++
Sbjct: 305 PVASPPPAAVPVSSPPAKSPPAPAPTAVPVAAPVTTPEVPAPAPSKTKKDAA 460
>TC14933 homologue to UP|TCMO_GLYEC (Q96423) Trans-cinnamate
4-monooxygenase (Cinnamic acid 4-hydroxylase) (CA4H)
(C4H) (P450C4H) (Cytochrome P450 73) , partial (57%)
Length = 917
Score = 32.0 bits (71), Expect = 0.015
Identities = 22/59 (37%), Positives = 32/59 (53%)
Frame = +2
Query: 30 SSTTTEPTISASPGVLPYVTSPDISSFFPTPMSSSEAPYEAEASAPAPAPSSGEKSSSS 88
++TTT IS+S V+S +SS P+P +S A + AP P+PSS S S+
Sbjct: 38 TTTTTSQWISSSWRRPS*VSSSPLSS--PSPSQNSAASVSSSLLAPPPSPSSATGSKSA 208
>TC15016 weakly similar to UP|Q8L685 (Q8L685) Pherophorin-dz1 protein
precursor, partial (4%)
Length = 948
Score = 31.6 bits (70), Expect = 0.020
Identities = 26/84 (30%), Positives = 38/84 (44%), Gaps = 2/84 (2%)
Frame = +1
Query: 7 TTIIKLLAFVFVVIILLDNSHAVSSTTTEPTISASPGVLPYVTSP--DISSFFPTPMSSS 64
TT+ F + +S + TTT +A P P +SP SS P P SSS
Sbjct: 34 TTLSLTSPFPTTITPTKTSSFSSQPTTTTTAAAAQP---PPASSPPSSSSSSSPPPPSSS 204
Query: 65 EAPYEAEASAPAPAPSSGEKSSSS 88
P AS+ + + +SG +S+ S
Sbjct: 205 THPTRKSASSESKSTTSGSESTRS 276
Score = 26.2 bits (56), Expect = 0.85
Identities = 17/44 (38%), Positives = 24/44 (53%), Gaps = 2/44 (4%)
Frame = +1
Query: 48 VTSPDISSFFPTPMSS--SEAPYEAEASAPAPAPSSGEKSSSST 89
+TSP ++ PT SS S+ A+A P P+S SSSS+
Sbjct: 46 LTSPFPTTITPTKTSSFSSQPTTTTTAAAAQPPPASSPPSSSSS 177
>TC10688 similar to UP|AAR10812 (AAR10812) Superoxide dismutase, partial
(91%)
Length = 822
Score = 31.6 bits (70), Expect = 0.020
Identities = 23/74 (31%), Positives = 34/74 (45%), Gaps = 6/74 (8%)
Frame = +2
Query: 39 SASPGVLPYVTSPDIS--SFFPTPMSSSEAPYEAEASAPAPAPSSGE----KSSSSTRLD 92
S P +P SP S S P P SS P + S+ AP PS K ++ +L
Sbjct: 98 SDHPSPVPPSNSPPTSEPSHPPNPSPSSPPPRKPSPSSRAPPPSKASSPSPKKTTDQQL* 277
Query: 93 CVAAIVGIMLFSVF 106
+ ++V + +F VF
Sbjct: 278 MLESLVSLQVFMVF 319
>TC13802 similar to UP|Q9M1T1 (Q9M1T1) Sugar-phosphate isomerase-like
protein, partial (41%)
Length = 522
Score = 31.2 bits (69), Expect = 0.026
Identities = 25/69 (36%), Positives = 33/69 (47%), Gaps = 2/69 (2%)
Frame = +2
Query: 25 NSHAVSSTTTEPTISASPGVLPYVTSPDISSFFPTPMSS--SEAPYEAEASAPAPAPSSG 82
N + +STT+ PT S +P P SP SS P SS S +P + +P P+ S
Sbjct: 104 NPNKTTSTTSSPT-STTPKPSP---SPAPSSTLRAPSSSPASASPASSPTKSPKPSSPSA 271
Query: 83 EKSSSSTRL 91
SST L
Sbjct: 272 SAPPSSTPL 298
>TC9037 similar to GB|AAG24276.1|10880493|AF195889 fasciclin-like
arabinogalactan protein FLA8 {Arabidopsis thaliana;} ,
partial (61%)
Length = 989
Score = 31.2 bits (69), Expect = 0.026
Identities = 20/64 (31%), Positives = 33/64 (51%)
Frame = +2
Query: 25 NSHAVSSTTTEPTISASPGVLPYVTSPDISSFFPTPMSSSEAPYEAEASAPAPAPSSGEK 84
+S V+ T + T + V + P++ + P+P AP ASAP+PAP+SG
Sbjct: 404 DSSRVADTVLDSTPLSIYSVDSVLLPPELFAKSPSP-----APAPEPASAPSPAPASGPS 568
Query: 85 SSSS 88
+S+
Sbjct: 569 PASA 580
Score = 26.6 bits (57), Expect = 0.65
Identities = 19/72 (26%), Positives = 29/72 (39%)
Frame = +2
Query: 31 STTTEPTISASPGVLPYVTSPDISSFFPTPMSSSEAPYEAEASAPAPAPSSGEKSSSSTR 90
S P +++P P S+ P P S E APA APS+ + S+S +
Sbjct: 506 SPAPAPEPASAPSPAPASGPSPASAPSPLPASPPAPAGETPDGAPADAPSTAAERSTSGK 685
Query: 91 LDCVAAIVGIML 102
V ++L
Sbjct: 686 SAGVTVKASVIL 721
>TC20065 similar to UP|AAR20725 (AAR20725) At5g18690, partial (21%)
Length = 592
Score = 31.2 bits (69), Expect = 0.026
Identities = 22/66 (33%), Positives = 30/66 (45%), Gaps = 10/66 (15%)
Frame = +3
Query: 12 LLAFVFVVIILLDNSHAVSSTTTEPTISASPGVL---------PY-VTSPDISSFFPTPM 61
L+ F+ LL + ST PT+S+SP L P+ V SPDIS P+P
Sbjct: 96 LIIMAFMPSPLLSSYSEAESTPRVPTLSSSPASLSDPPPSSLSPFQVLSPDISPLLPSPG 275
Query: 62 SSSEAP 67
+ P
Sbjct: 276 GALPTP 293
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.315 0.126 0.343
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,874,901
Number of Sequences: 28460
Number of extensions: 32868
Number of successful extensions: 856
Number of sequences better than 10.0: 353
Number of HSP's better than 10.0 without gapping: 672
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 790
length of query: 111
length of database: 4,897,600
effective HSP length: 87
effective length of query: 24
effective length of database: 2,421,580
effective search space: 58117920
effective search space used: 58117920
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 47 (22.7 bits)
Medicago: description of AC149131.7