BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149130.9 + phase: 0 /pseudo
(1214 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18710 164 8e-41
TC18198 weakly similar to UP|Q9XGZ5 (Q9XGZ5) T1N24.20 protein, p... 123 2e-28
BP085058 107 9e-24
AV780705 87 2e-17
AV767121 84 2e-16
AV768875 75 5e-14
CN825682 66 3e-11
BP060613 63 2e-10
TC19704 weakly similar to UP|Q9SZ87 (Q9SZ87) RNA-directed DNA po... 62 7e-10
AV780221 61 9e-10
TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%) 61 1e-09
TC12829 35 0.093
TC7819 similar to UP|Q43817 (Q43817) Lipoxygenase , partial (97%) 32 0.78
AV426437 31 1.3
BP071102 30 1.7
AV780392 30 3.0
AV768548 29 5.1
BP065135 28 6.6
BP028413 28 6.6
BP033834 28 8.7
>TC18710
Length = 843
Score = 164 bits (415), Expect = 8e-41
Identities = 90/217 (41%), Positives = 124/217 (56%), Gaps = 1/217 (0%)
Frame = +2
Query: 327 GPDGFHCIFFKQYWHIVGDDVFHLVRSAFLTGHFDPAISNTLIALIPKIDSPNTYKDFRP 386
G DG + +F+++ +V D V + + I+ TL+ LIPK+ P + FRP
Sbjct: 188 GMDGMNGLFYQKNGDVVKDSVT*AILAFLERAEIPNEINETLVTLIPKVPHPESINQFRP 367
Query: 387 ISLCNTLYKIITKVLVHRLRPFLNNLIGPYQSSFLPGRGTADNSIILQEILHFMKR-SKR 445
IS C LYK+I+K+ V RL+ + +LI P QS F+ GR DN +I+QE H + R
Sbjct: 368 ISGCTFLYKVISKIFVARLKDAMPDLISPMQSGFIQGRQIQDNLLIVQEAFHAINRPGAL 547
Query: 446 KKGYVAFKLDLEKAFDNVNWDFLNSCLLDFGFPDIIVKLIMHCVSSANYSLLWNGNKMPP 505
+ + KLD+ KA+D V W FL S LL FGF VK+IM VS +Y+ NG P
Sbjct: 548 GRNHSIIKLDMNKAYDRVEWKFLESSLLAFGFSTNWVKMIMILVSGVSYNYKINGVVGPK 727
Query: 506 FKPTHGLRQGDPLSPYLFILCMEKLSVAIQDAVLQGS 542
P GLRQGDP SPYLF+ ME LS+ IQ++ G+
Sbjct: 728 LLPQRGLRQGDPFSPYLFLFTMEVLSLLIQNSFNMGN 838
>TC18198 weakly similar to UP|Q9XGZ5 (Q9XGZ5) T1N24.20 protein, partial
(14%)
Length = 592
Score = 123 bits (308), Expect = 2e-28
Identities = 69/191 (36%), Positives = 105/191 (54%)
Frame = -3
Query: 225 VKLGDKNTAFFHAQTVIRRKWNKIHKLQLPNGISTSDSNILQEEALKYFKKFFCGSQIPY 284
+K GD NT FFHA T+ RR +N+I K++ +GI + E A+ YF + S + Y
Sbjct: 578 LK*GDHNTRFFHASTIQRRDFNRILKIKDAHGIWVEGQKKVNEAAVNYFSDIYSSSPLQY 399
Query: 285 SRFFNEGRHPALDDTGKTSLTSPITKKEVFAALNSMKPYKAPGPDGFHCIFFKQYWHIVG 344
R + D L + ++ E+ A+ SM KAPG DG + +F++Q IV
Sbjct: 398 LRECLAPIPKLVTDRLNHKLCAQVSTDEIKEAVFSMGDLKAPGMDGINGLFYQQNREIVK 219
Query: 345 DDVFHLVRSAFLTGHFDPAISNTLIALIPKIDSPNTYKDFRPISLCNTLYKIITKVLVHR 404
+DV + + F G ++ TL+ LIPKI + FRPIS C+ +YK+I+K++V R
Sbjct: 218 NDVNNAILDFFDHGELPVELNETLVTLIPKIPHAEAIQQFRPISCCSFIYKVISKIIVAR 39
Query: 405 LRPFLNNLIGP 415
L+P + NLI P
Sbjct: 38 LKPDMVNLISP 6
>BP085058
Length = 452
Score = 107 bits (268), Expect = 9e-24
Identities = 57/120 (47%), Positives = 77/120 (63%)
Frame = +2
Query: 612 NNLTAISGIQSTTTLGKYLGFPMLQGRPKRSDFNFILEKMQTRLASWKNRLLNRTGRLTL 671
+ + +I+ I T +GKY+ FP+ QG + DF F L+++ +RLA K LLN+ RLTL
Sbjct: 86 DRMVSITSIPFTDNIGKYMSFPIHQGHV*KEDFVFTLDRVISRLAC*KAYLLNKPARLTL 265
Query: 672 ATSVLSTIPTYYMQINWLPQNICDSIDQTARNFLWKGSNNKGIHLVNWKTITRPKSIGGL 731
A VLS I Y MQ+NWLPQ+ICD I R+F+W+ N GI LV W+TI KS+ GL
Sbjct: 266 AKPVLSAISMYVMQLNWLPQSICDQICTVVRHFVWENVN--GIPLVAWETIQSRKSMVGL 439
>AV780705
Length = 524
Score = 86.7 bits (213), Expect = 2e-17
Identities = 44/143 (30%), Positives = 68/143 (46%), Gaps = 2/143 (1%)
Frame = -2
Query: 627 GKYLGFPMLQGRPKRSDFNFILEKMQTRLASWKNRLLNRTGRLTLATSVLSTIPTYYMQI 686
G YLG P + GR K +I EK++ +L WK LLN+ G+ L +++ IP+Y M I
Sbjct: 490 GHYLGLPAIWGRNKSHSLVWIEEKVKEKLEGWKETLLNQAGKEVLIKAIIQAIPSYAMTI 311
Query: 687 NWLPQNICDSIDQTARNFLWKGSNNKGIHLVNWKTITRP--KSIGGLGIRSARDANICLL 744
P+ C+S+ +F WK + P K GG+G R R N+ L
Sbjct: 310 VHFPKTFCNSLYALVADFWWKSQGEGAVEYTGAVGTK*PLSKEKGGVGFRDLRTQNLAFL 131
Query: 745 GKLVWDMVQSTNKLWVNLLAKKY 767
+ W ++ + LWV ++ Y
Sbjct: 130 ARQAWRVLTNPEALWVRVMKSLY 62
>AV767121
Length = 525
Score = 83.6 bits (205), Expect = 2e-16
Identities = 54/177 (30%), Positives = 84/177 (46%), Gaps = 7/177 (3%)
Frame = +2
Query: 655 LASWKNRLLNRTGRLTLATSVLSTIPTYYMQINWLPQNICDSIDQTARNFLWKGSNNKG- 713
L+ WK + ++ GR+ L SVL+ +P +++ LP + S + RNFLW GS N+
Sbjct: 2 LSKWKQKTVSFGGRIFLIQSVLTALPLFFLSFFKLPIGVGKSCVRLMRNFLWGGSENENK 181
Query: 714 IHLVNWKTITRPKSIGGLGIRSARDANICLLGKLVWDMVQSTNKLWVNLLAKK---YSSG 770
I V W + +PK +GGLGI+ N LLGK W + + LW ++ + YS G
Sbjct: 182 IA*VKWTDVCKPKELGGLGIKDLFTFNKALLGK*RWRYLTEPDSLWRRVIEAQPDHYSCG 361
Query: 771 TSLLE--ANVNSNSSPSWFSIIRAKDILKTGYSWRAGAG-TSSFWFSNWSSHGYLGS 824
+S ++ + WFS +G G G + FW +W G L +
Sbjct: 362 SSWWNDILSLCPEDADGWFS---------SGLKKLVGEGDQTKFWSEDWLGSGLLSA 505
>AV768875
Length = 232
Score = 75.5 bits (184), Expect = 5e-14
Identities = 32/63 (50%), Positives = 43/63 (67%)
Frame = +1
Query: 1 INDTITGPWMLIGDFNETHLPSEQRGGTFHHNRAATFSNFMNNCNLLDLTTTGGRFTWHK 60
I +I+ PW+LIGD NE PS+ RGG FH NRA F+ +++CNL+DL GG+FTW +
Sbjct: 34 IRASISIPWLLIGDMNEILFPSQVRGGEFHPNRAQRFATVLDDCNLIDLGMVGGKFTWFR 213
Query: 61 NNN 63
N
Sbjct: 214KRN 222
>CN825682
Length = 626
Score = 66.2 bits (160), Expect = 3e-11
Identities = 35/95 (36%), Positives = 47/95 (48%), Gaps = 1/95 (1%)
Frame = -3
Query: 9 WMLIGDFNETHLPSEQRGGTFHH-NRAATFSNFMNNCNLLDLTTTGGRFTWHKNNNGIRI 67
W GDFNE E+ G R F +F++ NL+DL G RFTW N +
Sbjct: 300 WTCYGDFNEILHHQEKEGARDQDPTRIQVFRDFLDGANLMDLELKGCRFTWLSNLRNGLV 121
Query: 68 LSKKLDRGMANVDWRLSFPEAFVEVLCRLHSDHNP 102
++LDR + N WRL +P A L + SDH+P
Sbjct: 120 TKERLDRVLVNWTWRLDYPNATAMALPIISSDHSP 16
>BP060613
Length = 378
Score = 63.2 bits (152), Expect = 2e-10
Identities = 30/118 (25%), Positives = 52/118 (43%)
Frame = +1
Query: 690 PQNICDSIDQTARNFLWKGSNNKGIHLVNWKTITRPKSIGGLGIRSARDANICLLGKLVW 749
PQ++ + W +G+H W +T K GGLG + N LL K W
Sbjct: 1 PQDLXQGSMLSGCQVWWSSKGTRGVHWRKWDLLTELKDEGGLGFKDFEIQNQALLAKQAW 180
Query: 750 DMVQSTNKLWVNLLAKKYSSGTSLLEANVNSNSSPSWFSIIRAKDILKTGYSWRAGAG 807
++ + + LWV +L Y L+ + +S W S++ +++L W+ G+G
Sbjct: 181 RILHNPDALWVQILKALYFPHHDFLQTTKRTGASWVWSSLLHGRELLLKQGQWQLGSG 354
>TC19704 weakly similar to UP|Q9SZ87 (Q9SZ87) RNA-directed DNA
polymerase-like protein, partial (3%)
Length = 530
Score = 61.6 bits (148), Expect = 7e-10
Identities = 37/126 (29%), Positives = 66/126 (52%), Gaps = 8/126 (6%)
Frame = -3
Query: 135 VKRSW-------STHTHNPTASLIKVMENSIIFNHDVFGNIFQRKSRVEWRLKGVQSYLE 187
+K++W S+H N + ++H F + + ++ +L+ +Q+Y
Sbjct: 513 IKKAWDNTSETASSHCSNLSGKTTNTKLALTEWHHQNFSRADKEIAELKNQLEHLQNYSN 334
Query: 188 RVDSYRHTLLEK-ELQDEYNHILFQEEMLWYQKSREQWVKLGDKNTAFFHAQTVIRRKWN 246
+ ++ K L+D + QEE+ W Q+SR +W+K GDKNT FFHA TV RR+ N
Sbjct: 333 SGEDWQEIKQRKMRLKDLWR----QEELFWSQRSRIKWLKSGDKNTKFFHASTVQRRERN 166
Query: 247 KIHKLQ 252
+I +++
Sbjct: 165 RIERIK 148
>AV780221
Length = 461
Score = 61.2 bits (147), Expect = 9e-10
Identities = 47/158 (29%), Positives = 68/158 (42%), Gaps = 10/158 (6%)
Frame = +3
Query: 669 LTLATSVLSTIPTYYMQINWLPQN-ICDSIDQTARNFLWKG-SNNKGIHLVNWKTITRPK 726
L L SVL +P +++ LP +++ F + K I V W T+ RPK
Sbjct: 3 LCLIRSVLIALPLFHLPFFKLPTGGFYTNVNN**DPFFGREVEGGKKIAWVKWSTVCRPK 182
Query: 727 SIGGLGIRSARDANICLLGKLVWDMVQSTNKLWVNLLAKKYSSGTSLLEANVNSNSSPSW 786
GGLG+++ N LLGK W M++ + LW +L KY N S+ SW
Sbjct: 183 DEGGLGVQNLGLFNKALLGKWRWRMLKERDGLWYKVLLIKYK--------NAIPQSASSW 338
Query: 787 FSIIRAKDILKTGYSW-------RAGAGTS-SFWFSNW 816
++ + + G W R G GT FW NW
Sbjct: 339 WNDLHSTCFEDGGGGWMQRGLCRRIGEGTEVKFWNENW 452
>TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%)
Length = 912
Score = 60.8 bits (146), Expect = 1e-09
Identities = 37/123 (30%), Positives = 60/123 (48%)
Frame = -3
Query: 554 QISHLLFADDVLLFTKAKSSQLQFITNLFDRFSRASGLKINISKSRAYYSSGTPNGKINN 613
+ISHL FAD++++F+ + I N+ F SGL N +KS + N
Sbjct: 376 EISHLCFADNLMVFSNGYLESIAIINNVLHIFQHLSGLTPNPAKSEVFIL--VKETTKNQ 203
Query: 614 LTAISGIQSTTTLGKYLGFPMLQGRPKRSDFNFILEKMQTRLASWKNRLLNRTGRLTLAT 673
+T + G + + LG P L + K SD ++++ ++L W R L+ GRL L
Sbjct: 202 ITNMLGYKEGKLPVR*LGVPQLTTKLKASDCKVLVDRKPSKLQHWTGRSLSYAGRLQLIN 23
Query: 674 SVL 676
S+L
Sbjct: 22 SIL 14
>TC12829
Length = 448
Score = 34.7 bits (78), Expect = 0.093
Identities = 18/44 (40%), Positives = 21/44 (46%)
Frame = +3
Query: 661 RLLNRTGRLTLATSVLSTIPTYYMQINWLPQNICDSIDQTARNF 704
+ L R GR L SV IPTY M LP +IC I+ F
Sbjct: 255 KFLYRAGREVLIKSVTKAIPTYIMSCFALPDSICSQIEGMVGKF 386
>TC7819 similar to UP|Q43817 (Q43817) Lipoxygenase , partial (97%)
Length = 2842
Score = 31.6 bits (70), Expect = 0.78
Identities = 22/80 (27%), Positives = 36/80 (44%), Gaps = 2/80 (2%)
Frame = +2
Query: 242 RRKWNKIHKLQLPNGISTSDSNILQEEALKYFKKFFCGSQIPYSRFFNEGRHPALDDTGK 301
R++W++++ + N + DSNI + + S +PY R GR PA D
Sbjct: 677 RKEWDRVYDYDVYNDLGDPDSNIRLKRPV-----LGGNSDLPYPRRGRTGRKPAKKDPQS 841
Query: 302 TSLTS--PITKKEVFAALNS 319
S +S + + E F L S
Sbjct: 842 ESRSSFFYLPRDEAFGHLKS 901
>AV426437
Length = 303
Score = 30.8 bits (68), Expect = 1.3
Identities = 11/26 (42%), Positives = 19/26 (72%)
Frame = +1
Query: 710 NNKGIHLVNWKTITRPKSIGGLGIRS 735
N + I +NW+TI +PK + GLG+++
Sbjct: 217 NQRKIAWINWETICKPKWLRGLGVKN 294
>BP071102
Length = 450
Score = 30.4 bits (67), Expect = 1.7
Identities = 17/58 (29%), Positives = 28/58 (47%), Gaps = 1/58 (1%)
Frame = +2
Query: 114 RGPRPFRFEAAWIDHYDYGNVVKRSWSTHTH-NPTASLIKVMENSIIFNHDVFGNIFQ 170
R PR FRF + +DH Y K+ + T H P + + V N +++ V+ +Q
Sbjct: 2 RAPRIFRFSSKHLDHIKYRMSEKQPYRTSYHFQPQQNWMNVDPNGPMYHKGVYHFFYQ 175
>AV780392
Length = 534
Score = 29.6 bits (65), Expect = 3.0
Identities = 10/25 (40%), Positives = 17/25 (68%)
Frame = -3
Query: 792 AKDILKTGYSWRAGAGTSSFWFSNW 816
A + ++ G+S+R G +S W+SNW
Sbjct: 400 ASNFVRDGFSFRLQPGNTSRWYSNW 326
>AV768548
Length = 254
Score = 28.9 bits (63), Expect = 5.1
Identities = 16/43 (37%), Positives = 24/43 (55%), Gaps = 8/43 (18%)
Frame = -2
Query: 811 FWFSN----WSSHGYLGS----LVPIIDIHDIHLTVKDVLTSV 845
FW + W+S G+L + L+P ID+H +HL + D SV
Sbjct: 226 FWSEDFAYIWNSLGFLCAFSQWLLPTIDVHYLHLILDDYYLSV 98
>BP065135
Length = 492
Score = 28.5 bits (62), Expect = 6.6
Identities = 12/37 (32%), Positives = 16/37 (42%)
Frame = +2
Query: 487 HCVSSANYSLLWNGNKMPPFKPTHGLRQGDPLSPYLF 523
HC SS + W GNK + Q + L P+ F
Sbjct: 164 HCKSSVASGIFWQGNKARTLVDVELMEQPEQLKPFSF 274
>BP028413
Length = 528
Score = 28.5 bits (62), Expect = 6.6
Identities = 17/68 (25%), Positives = 32/68 (47%)
Frame = +2
Query: 754 STNKLWVNLLAKKYSSGTSLLEANVNSNSSPSWFSIIRAKDILKTGYSWRAGAGTSSFWF 813
S LW NL ++ + L+E N + + S + + +KT SW +G +
Sbjct: 95 SLTLLWANLCLQQLKTTNLLIETNYQ*SPTKSEDME*KGE*CMKTDVSWVSGFQLLQEFI 274
Query: 814 SNWSSHGY 821
+++SSH +
Sbjct: 275 NSFSSHQF 298
>BP033834
Length = 534
Score = 28.1 bits (61), Expect = 8.7
Identities = 13/28 (46%), Positives = 16/28 (56%)
Frame = +1
Query: 710 NNKGIHLVNWKTITRPKSIGGLGIRSAR 737
+ G HLV+W T K+ GLG R AR
Sbjct: 10 SRNG*HLVSWGKATHVKNY*GLGFREAR 93
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.331 0.143 0.457
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,376,072
Number of Sequences: 28460
Number of extensions: 354749
Number of successful extensions: 1996
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 1972
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1992
length of query: 1214
length of database: 4,897,600
effective HSP length: 101
effective length of query: 1113
effective length of database: 2,023,140
effective search space: 2251754820
effective search space used: 2251754820
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 61 (28.1 bits)
Medicago: description of AC149130.9