BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149127.2 + phase: 0
(458 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP045360 158 2e-39
AV417757 89 1e-18
AV424056 66 1e-11
AV411613 60 6e-10
AV779982 60 1e-09
AU089238 59 1e-09
TC12717 similar to UP|Q93ZH7 (Q93ZH7) AT5g03040/F15A17_70, parti... 47 7e-06
AV406990 47 9e-06
TC18405 42 2e-04
TC15040 similar to UP|K22E_HUMAN (P35908) Keratin, type II cytos... 34 0.058
BP043442 33 0.075
AV768808 33 0.098
TC20195 similar to UP|Q8IQS0 (Q8IQS0) CG32193-PA, partial (5%) 32 0.17
BP073425 32 0.29
AV767934 31 0.37
TC17900 similar to UP|BAD10560 (BAD10560) Calmodulin-binding pro... 30 0.64
AV416576 30 1.1
TC11144 similar to UP|Q9XF71 (Q9XF71) CLC-Nt2 protein, partial (... 29 1.4
TC17226 similar to UP|Q84P55 (Q84P55) TGB12K interacting protein... 29 1.4
TC8558 weakly similar to UP|Q9NDI0 (Q9NDI0) 200 kDa antigen p200... 29 1.4
>BP045360
Length = 497
Score = 158 bits (399), Expect = 2e-39
Identities = 84/136 (61%), Positives = 99/136 (72%), Gaps = 2/136 (1%)
Frame = -2
Query: 324 TPSRGNFNMGPMGLMAQEFHDSSPT-FNRQHQRPPSPGRPSYMAPTQSAKAKVRAEGPFK 382
TP + NMG L Q+FHDSS + +NRQHQR PSP RPSYMAPTQSAKAK R +GP K
Sbjct: 496 TPGPSSINMG---LTNQDFHDSSLSPYNRQHQRLPSPNRPSYMAPTQSAKAKSRVQGPGK 326
Query: 383 QRAPYGPNWNSSIKGGSIIGSGCDSSSSGGGTSAYKFPRSPGPKVNGVRSESRRTVGGSQ 442
+ + + NSS KGGS IGSGCDSSSSG G + ++F SP PKVNG+ S SRR +GGS+
Sbjct: 325 PQVSFVSHSNSSTKGGSNIGSGCDSSSSGVGNTTHQFLSSPSPKVNGMHSHSRRNLGGSR 146
Query: 443 -DYTEDWAVPLGAHGW 457
D ED A+PLGAHGW
Sbjct: 145 DDLVED*ALPLGAHGW 98
>AV417757
Length = 253
Score = 89.4 bits (220), Expect = 1e-18
Identities = 47/69 (68%), Positives = 57/69 (82%)
Frame = +1
Query: 94 KEERAATFIQSHYRGYLARRALRALKGLVRLQALVRGHNVRKQAQMTMRCMQALVRVQAR 153
K+E AA IQ+ +RG+LARRALRAL+ +VRLQA+ RG VRKQA +T+RCMQALVRVQAR
Sbjct: 13 KQEWAAIRIQTLFRGFLARRALRALRAVVRLQAIFRGRLVRKQAAVTLRCMQALVRVQAR 192
Query: 154 VRARRVQLS 162
V+AR V S
Sbjct: 193 VKARNVSKS 219
>AV424056
Length = 193
Score = 66.2 bits (160), Expect = 1e-11
Identities = 40/64 (62%), Positives = 45/64 (69%), Gaps = 5/64 (7%)
Frame = +1
Query: 62 VAEATAAAASAAVVAAQAAARVVRLAGYGRH-----NKEERAATFIQSHYRGYLARRALR 116
VA ATAAAA AAV AAQAAA VVRL +GR + AA IQ+ +RGYLAR+ALR
Sbjct: 1 VAAATAAAADAAVAAAQAAAAVVRLTSHGRDTMFGGGHQRLAAVKIQTVFRGYLARKALR 180
Query: 117 ALKG 120
ALKG
Sbjct: 181 ALKG 192
>AV411613
Length = 430
Score = 60.5 bits (145), Expect = 6e-10
Identities = 29/78 (37%), Positives = 48/78 (61%)
Frame = +2
Query: 205 WDNKCQSVKKAKENDLRKHEAAMKRERALAYAFNYQQQQQKQHLHRNSNGDDVDMGTYHP 264
WD+ QS ++ + L K+EA M+RERA+AY+F++QQ + + + + + M +P
Sbjct: 50 WDDSLQSKEQVEAKLLSKYEATMRRERAMAYSFSHQQNKDS-----SKSINPMFMDPTNP 214
Query: 265 NDDEKVQWGWNWLERWMS 282
+ WGW+WLERWM+
Sbjct: 215 S------WGWSWLERWMA 250
>AV779982
Length = 516
Score = 59.7 bits (143), Expect = 1e-09
Identities = 28/44 (63%), Positives = 36/44 (81%)
Frame = +2
Query: 109 YLARRALRALKGLVRLQALVRGHNVRKQAQMTMRCMQALVRVQA 152
YLARRA RALKG++R QAL+RGH VR+QA T+ CM +V++QA
Sbjct: 377 YLARRAFRALKGIIRSQALMRGHLVRRQAVSTLCCMYGMVKLQA 508
>AU089238
Length = 473
Score = 59.3 bits (142), Expect = 1e-09
Identities = 40/148 (27%), Positives = 66/148 (44%)
Frame = +2
Query: 132 NVRKQAQMTMRCMQALVRVQARVRARRVQLSHECLEKAMEEDEEEEFVRQHETITKPMSP 191
NV++Q M+ MQ LVRVQ+++++RR+Q+ + + EF + +
Sbjct: 11 NVKRQTVNAMKHMQLLVRVQSQIQSRRIQMLEN------QARYQAEFKNDKDAASTFGKR 172
Query: 192 MRRSSVSSNNNNGWDNKCQSVKKAKENDLRKHEAAMKRERALAYAFNYQQQQQKQHLHRN 251
+ NN WD+ ++ RK EA +KRERA+A+A+++Q + +
Sbjct: 173 TLGHGSEAGNNEEWDDSILKKEEVDARLQRKVEAMIKRERAMAFAYSHQLWKATPKSTQT 352
Query: 252 SNGDDVDMGTYHPNDDEKVQWGWNWLER 279
D G W WNWL R
Sbjct: 353 PVTDTRSGG---------FPWWWNWLXR 409
>TC12717 similar to UP|Q93ZH7 (Q93ZH7) AT5g03040/F15A17_70, partial (20%)
Length = 390
Score = 47.0 bits (110), Expect = 7e-06
Identities = 30/65 (46%), Positives = 38/65 (58%)
Frame = +3
Query: 61 AVAEATAAAASAAVVAAQAAARVVRLAGYGRHNKEERAATFIQSHYRGYLARRALRALKG 120
A E A + AAVV A A + K+E AA IQ+ +RG+LARRALRAL+G
Sbjct: 18 AAPEEPAPSVQAAVVRIGAGTN----AQFAGKPKDEVAAIKIQTAFRGHLARRALRALRG 185
Query: 121 LVRLQ 125
LVR +
Sbjct: 186 LVRFE 200
>AV406990
Length = 392
Score = 46.6 bits (109), Expect = 9e-06
Identities = 29/53 (54%), Positives = 34/53 (63%), Gaps = 3/53 (5%)
Frame = +3
Query: 37 SPDDVTNEGSTTSTPVRDD---RNHAIAVAEATAAAASAAVVAAQAAARVVRL 86
SP + + ST+S+P D HAIAVA ATAA A AA+ AA AAA VVRL
Sbjct: 189 SPLNHQFDSSTSSSPFTDSLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRL 347
>TC18405
Length = 542
Score = 42.0 bits (97), Expect = 2e-04
Identities = 21/47 (44%), Positives = 32/47 (67%)
Frame = +2
Query: 95 EERAATFIQSHYRGYLARRALRALKGLVRLQALVRGHNVRKQAQMTM 141
E AA IQ+ +R Y AR+ALR LK +L+ ++G++V+KQA T+
Sbjct: 380 ETIAAIRIQTAFRAYKARKALRRLKRFTKLKVQIQGYSVKKQAGTTI 520
>TC15040 similar to UP|K22E_HUMAN (P35908) Keratin, type II cytoskeletal 2
epidermal (Cytokeratin 2e) (K2e) (CK 2e), partial (3%)
Length = 573
Score = 33.9 bits (76), Expect = 0.058
Identities = 29/93 (31%), Positives = 41/93 (43%), Gaps = 2/93 (2%)
Frame = +3
Query: 337 LMAQEFHDSSPTFNRQHQRPPSPGRPSYMAPTQSAKAKVRAEGPFKQRAPYGPNWNSSIK 396
L A + +P Q P +P P+Y +P + A ++ AP ++NSS
Sbjct: 99 LQAMSYGRYNPQSPSQSYAPSAPELPAYSSPPPHSAAYTQSPS----HAPPHSSYNSSHG 266
Query: 397 GGSI--IGSGCDSSSSGGGTSAYKFPRSPGPKV 427
G SG SSSGGG S+ FP P+V
Sbjct: 267 YGQAPPSSSGYPPSSSGGGYSS--FPPGTHPEV 359
>BP043442
Length = 564
Score = 33.5 bits (75), Expect = 0.075
Identities = 27/93 (29%), Positives = 45/93 (48%), Gaps = 3/93 (3%)
Frame = -1
Query: 362 PSYMAPTQSAKAKVRAEGPFKQRAPYGPNWNSSIKGGSIIGSGCDSSSSGGGTSAYKFPR 421
PSYMAPT+SAKA++R +G P + + + + + SSS S PR
Sbjct: 558 PSYMAPTESAKARLRGQG--------SPRFATDLADKNSMTRRHSLSSSFNSKSGSFSPR 403
Query: 422 SPGPKVNG---VRSESRRTVGGSQDYTEDWAVP 451
+ + G +R++ R++ S+D T+ P
Sbjct: 402 AELISMGGRGVIRTD--RSLSSSRDGTDKMMQP 310
>AV768808
Length = 537
Score = 33.1 bits (74), Expect = 0.098
Identities = 14/28 (50%), Positives = 21/28 (75%)
Frame = -1
Query: 360 GRPSYMAPTQSAKAKVRAEGPFKQRAPY 387
G P+YMA T+S+KAKVR++ +QR +
Sbjct: 456 GHPNYMANTESSKAKVRSQSAPRQRLEF 373
>TC20195 similar to UP|Q8IQS0 (Q8IQS0) CG32193-PA, partial (5%)
Length = 591
Score = 32.3 bits (72), Expect = 0.17
Identities = 17/65 (26%), Positives = 32/65 (49%)
Frame = -1
Query: 191 PMRRSSVSSNNNNGWDNKCQSVKKAKENDLRKHEAAMKRERALAYAFNYQQQQQKQHLHR 250
P R ++ +++N+N + + ++ +H+ + A A A N QQQQ+QH +
Sbjct: 420 PFRVTTATTHNSNIT*QQSKDSSSVYQHQQGQHQQPARTAPAPAPASNTYQQQQRQHQRQ 241
Query: 251 NSNGD 255
N D
Sbjct: 240 QKNSD 226
>BP073425
Length = 392
Score = 31.6 bits (70), Expect = 0.29
Identities = 22/88 (25%), Positives = 35/88 (39%)
Frame = -3
Query: 31 HFPAESSPDDVTNEGSTTSTPVRDDRNHAIAVAEATAAAASAAVVAAQAAARVVRLAGYG 90
HF A S +++ GS + +P DR+H A A A+ + A G
Sbjct: 363 HFLANRSASSLSHAGSPSPSPHHPDRSHHQMDALAVASRGRGPIQGRGKGAETRHEEGGP 184
Query: 91 RHNKEERAATFIQSHYRGYLARRALRAL 118
R ++ERA + + G + R L
Sbjct: 183 RRAQKERARAAPREEWGGGVGEGGFRTL 100
>AV767934
Length = 386
Score = 31.2 bits (69), Expect = 0.37
Identities = 13/19 (68%), Positives = 15/19 (78%)
Frame = -1
Query: 362 PSYMAPTQSAKAKVRAEGP 380
PSYM PT+SAKAK R + P
Sbjct: 377 PSYMVPTKSAKAKSRMQSP 321
>TC17900 similar to UP|BAD10560 (BAD10560) Calmodulin-binding protein
family-like, partial (4%)
Length = 629
Score = 30.4 bits (67), Expect = 0.64
Identities = 13/17 (76%), Positives = 16/17 (93%)
Frame = +2
Query: 362 PSYMAPTQSAKAKVRAE 378
PSYMA T+SAKAK+RA+
Sbjct: 5 PSYMAATESAKAKLRAQ 55
>AV416576
Length = 424
Score = 29.6 bits (65), Expect = 1.1
Identities = 11/28 (39%), Positives = 20/28 (71%)
Frame = +1
Query: 360 GRPSYMAPTQSAKAKVRAEGPFKQRAPY 387
G P+YM+ T+S +AK+R++ +QR +
Sbjct: 331 GHPNYMSNTESYRAKIRSQSAPRQRVEF 414
>TC11144 similar to UP|Q9XF71 (Q9XF71) CLC-Nt2 protein, partial (14%)
Length = 557
Score = 29.3 bits (64), Expect = 1.4
Identities = 22/80 (27%), Positives = 36/80 (44%), Gaps = 5/80 (6%)
Frame = +1
Query: 163 HECLEKAMEEDEEEEFVRQHETITKPMSPMRRSSVSSNNNNGWDNKCQSVK----KAKEN 218
H +E+ +E +EEE + + +P+ R ++SSN K ++ + EN
Sbjct: 151 HNKMEEVVEREEEEVIDPEGNPLNQPLLIKRNRTLSSNPLALVGAKVSYIESLDYEINEN 330
Query: 219 DLRKHE-AAMKRERALAYAF 237
DL KH + R L Y F
Sbjct: 331 DLFKHNWRSRSRVEVLQYIF 390
>TC17226 similar to UP|Q84P55 (Q84P55) TGB12K interacting protein 3, partial
(48%)
Length = 769
Score = 29.3 bits (64), Expect = 1.4
Identities = 17/67 (25%), Positives = 29/67 (42%)
Frame = +1
Query: 359 PGRPSYMAPTQSAKAKVRAEGPFKQRAPYGPNWNSSIKGGSIIGSGCDSSSSGGGTSAYK 418
P + P ++++ ++ P + G ++ + K GS S D SSS + K
Sbjct: 4 PPHTHTLPPYRTSQIQLFLSSPSLMASDSGKDFPADDKAGSAEKSSKDESSSKDSPAGQK 183
Query: 419 FPRSPGP 425
P PGP
Sbjct: 184 APSGPGP 204
>TC8558 weakly similar to UP|Q9NDI0 (Q9NDI0) 200 kDa antigen p200
(Fragment), partial (4%)
Length = 585
Score = 29.3 bits (64), Expect = 1.4
Identities = 21/58 (36%), Positives = 28/58 (48%), Gaps = 3/58 (5%)
Frame = +1
Query: 123 RLQALVRGHNVRKQAQMTMRCMQALVRVQARVRARRVQ---LSHECLEKAMEEDEEEE 177
RL A R +N+R + + AL QAR R RR + L+ E E + EEEE
Sbjct: 31 RLDAEERRNNIRLREEQDAAYRAALEADQARERQRREEQERLAREAAEAERKRKEEEE 204
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.314 0.127 0.376
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,788,018
Number of Sequences: 28460
Number of extensions: 108544
Number of successful extensions: 1100
Number of sequences better than 10.0: 73
Number of HSP's better than 10.0 without gapping: 1056
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1086
length of query: 458
length of database: 4,897,600
effective HSP length: 93
effective length of query: 365
effective length of database: 2,250,820
effective search space: 821549300
effective search space used: 821549300
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 57 (26.6 bits)
Medicago: description of AC149127.2