BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149079.11 - phase: 0
(664 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18230 similar to UP|Q945U3 (Q945U3) Acyl-CoA oxidase , partia... 648 0.0
BP054097 191 4e-49
TC12129 similar to UP|Q945U3 (Q945U3) Acyl-CoA oxidase , partia... 184 5e-47
AV768304 116 1e-26
TC18998 similar to UP|Q945U4 (Q945U4) Acyl-CoA oxidase , partia... 99 2e-21
TC9455 similar to UP|Q9LKX5 (Q9LKX5) Acyl-CoA oxidase ACX3 (At1... 99 3e-21
BP041900 74 6e-14
TC11908 similar to PIR|T46895|T46895 acyl-CoA dehydrogenase [va... 48 4e-06
AV425562 42 2e-05
TC14835 similar to PIR|T46895|T46895 acyl-CoA dehydrogenase [va... 29 2.1
TC14538 similar to GB|AAH54382.1|32450414|BC054382 feminization ... 28 3.6
TC9632 similar to PIR|T47546|T47546 protein kinase-like - Arabid... 28 4.7
TC14281 similar to UP|Q9XHC6 (Q9XHC6) Cytochrome P450 monooxygen... 28 6.2
AV766460 27 8.1
BP073048 27 8.1
AV770176 27 8.1
>TC18230 similar to UP|Q945U3 (Q945U3) Acyl-CoA oxidase , partial (53%)
Length = 1222
Score = 648 bits (1671), Expect = 0.0
Identities = 315/352 (89%), Positives = 334/352 (94%)
Frame = +1
Query: 35 SDRMSRLVASDPAFRKDHRPMLGRKELFKSTLKKAAYAWKRIIELRLTEEEASMLRSFVD 94
SDR+SRLVASDPAFRKD R LGRK+LFK+TL+K AYAWKRI+ELRL E+EA+ LRSFVD
Sbjct: 1 SDRISRLVASDPAFRKDDRTTLGRKDLFKNTLRKTAYAWKRILELRLNEKEAAKLRSFVD 180
Query: 95 EPAFTDLHWGMFIPAIKGQGTEEQQQKWLPLAQRMQIIGCYAQTELGHGSNVQGLETTAT 154
EPAFTDLHWGMF+PAIKGQGT+EQQQKWLPLA +MQIIGCYAQTELGHGSNVQGLETTAT
Sbjct: 181 EPAFTDLHWGMFVPAIKGQGTDEQQQKWLPLAYKMQIIGCYAQTELGHGSNVQGLETTAT 360
Query: 155 FDPKTDEFVIHSPTLTSSKWWPGGLGKVSTHAVVYARLITDGRDHGVHGFIVQLRSLDDH 214
FDPKTDEFVIHSPTLTSSKWWPGGLGK+STHAVVYARLITDG++ GVHGFIVQLRSLDDH
Sbjct: 361 FDPKTDEFVIHSPTLTSSKWWPGGLGKISTHAVVYARLITDGQEQGVHGFIVQLRSLDDH 540
Query: 215 LPLPGITVGDIGMKFGNGAYNTMDNGVLRFEHVRIPRDQMLMRVSQVTREGKYVQSNVPR 274
LPLPGITVGDIGMKFG+GAYNTMDNGVLRF+HVRIPR+QMLMRVSQVTREGKYVQSNVPR
Sbjct: 541 LPLPGITVGDIGMKFGSGAYNTMDNGVLRFDHVRIPRNQMLMRVSQVTREGKYVQSNVPR 720
Query: 275 QLVYGTMVYVRQTIVSDASTAMSRAVCIAARYSAVRRQFGGNNGSLESQVIDYKTQQARL 334
QLVYGTMVYVRQ IVSDAS A+SRAVCIA RYSAVRRQFG +NG LESQVIDYKTQQARL
Sbjct: 721 QLVYGTMVYVRQAIVSDASIALSRAVCIATRYSAVRRQFGSHNGGLESQVIDYKTQQARL 900
Query: 335 FPLLASAYAFRFVGEWLKWLYTDVMKRLQANDFSTLPEAHACTAGLKSLTTS 386
FPLLAS YAFRFV EWL WLYTDV +RLQA DFSTLPEAHACTAGLKSLTTS
Sbjct: 901 FPLLASTYAFRFVSEWLSWLYTDVTQRLQAGDFSTLPEAHACTAGLKSLTTS 1056
>BP054097
Length = 487
Score = 191 bits (484), Expect = 4e-49
Identities = 88/101 (87%), Positives = 95/101 (93%)
Frame = -2
Query: 564 LHKHLGDFLSTGCITPKQGSLANDQLRSLYSQVRPNAIALVDAFNYTDHYLSSVLGRYDG 623
LHKHLGDFLS CI P QGSLAN+QLRSLYSQVRPNAIALVDAFNYTDHYL SVLGRYDG
Sbjct: 477 LHKHLGDFLSAACINPLQGSLANEQLRSLYSQVRPNAIALVDAFNYTDHYLGSVLGRYDG 298
Query: 624 NVYPKLYEEAWKDPLNDSVVPDGFQEYIRPMLKQQLRNARL 664
NVYP+LYEEAWKDPLNDSV+PDGF +Y++P+LKQQLRNARL
Sbjct: 297 NVYPQLYEEAWKDPLNDSVIPDGFHQYVQPILKQQLRNARL 175
>TC12129 similar to UP|Q945U3 (Q945U3) Acyl-CoA oxidase , partial (17%)
Length = 343
Score = 184 bits (466), Expect = 5e-47
Identities = 92/113 (81%), Positives = 100/113 (88%)
Frame = +1
Query: 433 QVARHLMKVISRLGSGKKPVGTTAYMGRVEQLLEARSDVQKAEDWLNPNTVLRAFEARAA 492
QVARHL+K IS+L SGKKP GTTAYMGR+EQL++ RSDVQKAEDWL PN VL AFEARAA
Sbjct: 1 QVARHLIKTISQLSSGKKPGGTTAYMGRLEQLMQFRSDVQKAEDWLKPNVVLGAFEARAA 180
Query: 493 RMSVACAKNLTKFSNPEEGFQELLADLVDAARAHCQLIVVSKFIEKLQQDIPG 545
SV CAKNL+KF+NPEEGFQEL ADL +AA AHCQLIVVSKFIEKLQQDIPG
Sbjct: 181 MKSVVCAKNLSKFTNPEEGFQELSADLAEAAVAHCQLIVVSKFIEKLQQDIPG 339
>AV768304
Length = 426
Score = 116 bits (291), Expect = 1e-26
Identities = 54/58 (93%), Positives = 55/58 (94%)
Frame = -2
Query: 607 FNYTDHYLSSVLGRYDGNVYPKLYEEAWKDPLNDSVVPDGFQEYIRPMLKQQLRNARL 664
FNYTDHYL SVLGRYDG+VYPKL EEAWKDPLNDSVVPDGFQEYIRPMLKQQL NARL
Sbjct: 425 FNYTDHYLGSVLGRYDGDVYPKLSEEAWKDPLNDSVVPDGFQEYIRPMLKQQLGNARL 252
>TC18998 similar to UP|Q945U4 (Q945U4) Acyl-CoA oxidase , partial (9%)
Length = 186
Score = 99.4 bits (246), Expect = 2e-21
Identities = 47/61 (77%), Positives = 55/61 (90%)
Frame = -3
Query: 462 EQLLEARSDVQKAEDWLNPNTVLRAFEARAARMSVACAKNLTKFSNPEEGFQELLADLVD 521
EQL++ SDV+KAEDWL PN V+ AFEARAARMSVACA+NL+KF+NPEEGFQEL ADLV+
Sbjct: 184 EQLMQYHSDVKKAEDWLKPNVVVAAFEARAARMSVACAQNLSKFTNPEEGFQELTADLVE 5
Query: 522 A 522
A
Sbjct: 4 A 2
>TC9455 similar to UP|Q9LKX5 (Q9LKX5) Acyl-CoA oxidase ACX3 (At1g06290) ,
partial (50%)
Length = 1361
Score = 98.6 bits (244), Expect = 3e-21
Identities = 84/340 (24%), Positives = 155/340 (44%), Gaps = 11/340 (3%)
Frame = +3
Query: 289 VSDASTAMSR-AVCIAARYSAVRRQFGGNNGSLESQVIDYKTQQARLFPLLASAYAFRFV 347
+S ++ MS+ ++ IA RY+ R+ F E ++DY + Q RL PLLA YA
Sbjct: 24 ISVSAVYMSKVSLAIAIRYALTRQAFSITPNGPEVLLLDYPSHQRRLLPLLAKVYAMSLA 203
Query: 348 GEWLKWLYTDVMKRLQANDFSTLPEAHACTAGLKSLTTSATSDGIEECRKLCGGHGYLCS 407
LK +Y +KR ++ E H ++ K+ T ++ECR+ CGG G
Sbjct: 204 AFELKMMY---VKRTPESN----KEIHIVSSAYKATFTWNNMRTLQECREACGGQGIKTE 362
Query: 408 SGLPELFAVYVPTCTYEGDNIVLLLQVARHLMKVISRLGSGKKPVGTTA--YMGRVEQLL 465
+ + + + T+EGDN VL+ Q+++ L+ KP +M + ++
Sbjct: 363 NRIGQFKGEFDVQSTFEGDNNVLMQQISKALLAEYIACQMKNKPFSGLGLEHMNKPCPVI 542
Query: 466 EAR--------SDVQKAEDWLNPNTVLRAFEARAARMSVACAKNLTKFSNPEEGFQELLA 517
++ SD Q L +L+ F A +++ ++ + E F
Sbjct: 543 PSQLTSSNLRSSDFQIDLFHLRERDLLKRFAAEV-------SEHQSRGESKESAFILSYQ 701
Query: 518 DLVDAARAHCQLIVVSKFIEKLQQDIPGKGVKRQLEILCNIYALFHLHKHLGDFLSTGCI 577
D RA + ++ F+E + +P +K L +L ++YAL + + FL G +
Sbjct: 702 LAEDLGRAFSERAILKTFVE-TESALPAGSLKNVLGLLRSLYALICVDEDAA-FLRYGYL 875
Query: 578 TPKQGSLANDQLRSLYSQVRPNAIALVDAFNYTDHYLSSV 617
+ + + ++ L +++RP+A+ALV +F D +LS +
Sbjct: 876 STQNAAAVRKEVPKLCAELRPHALALVSSFGIPDAFLSPI 995
>BP041900
Length = 282
Score = 74.3 bits (181), Expect = 6e-14
Identities = 43/94 (45%), Positives = 50/94 (52%), Gaps = 6/94 (6%)
Frame = -2
Query: 153 ATFDPKTDEFVIHSPTLTSSKWWPGGLGKVSTHAVVYARLITDG------RDHGVHGFIV 206
ATFDP TDEFVI +P + KWW G A V+ARL D GVH FIV
Sbjct: 278 ATFDPITDEFVIDTPNDGAIKWWIGNAAVHGKFATVFARLRLPNYDAKGVSDMGVHAFIV 99
Query: 207 QLRSLDDHLPLPGITVGDIGMKFGNGAYNTMDNG 240
+R + H PLPGI + D G K G N +DNG
Sbjct: 98 PIRDMKTHKPLPGIEIHDCGHKVG---LNGVDNG 6
>TC11908 similar to PIR|T46895|T46895 acyl-CoA dehydrogenase [validated] -
Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (36%)
Length = 512
Score = 48.1 bits (113), Expect = 4e-06
Identities = 42/120 (35%), Positives = 62/120 (51%), Gaps = 3/120 (2%)
Frame = +2
Query: 114 GTEEQQQKWLPLAQRMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSK 173
G+E Q++K+LP +++ I C+A TE +GS+ L+TTAT K + I L K
Sbjct: 41 GSEAQKEKYLPSLAQLETIACWALTEPDYGSDASALKTTAT---KVEGGWI----LEGQK 199
Query: 174 WWPGGLGKVSTHA---VVYARLITDGRDHGVHGFIVQLRSLDDHLPLPGITVGDIGMKFG 230
W G ST A VV+AR T + ++G+IV+ + PG+TV I K G
Sbjct: 200 RWIGN----STFADLLVVFARNATTNQ---INGYIVKKDA-------PGLTVTKIENKIG 337
>AV425562
Length = 431
Score = 41.6 bits (96), Expect(2) = 2e-05
Identities = 20/30 (66%), Positives = 22/30 (72%)
Frame = +2
Query: 413 LFAVYVPTCTYEGDNIVLLLQVARHLMKVI 442
LFAV +P CTY+GDNIVLLLQV K I
Sbjct: 152 LFAVSIPACTYKGDNIVLLLQVIHEY*KKI 241
Score = 23.9 bits (50), Expect(2) = 2e-05
Identities = 9/14 (64%), Positives = 10/14 (71%)
Frame = +1
Query: 388 TSDGIEECRKLCGG 401
+ D I ECRKLC G
Sbjct: 106 SKDAIGECRKLCTG 147
>TC14835 similar to PIR|T46895|T46895 acyl-CoA dehydrogenase [validated] -
Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (31%)
Length = 741
Score = 29.3 bits (64), Expect = 2.1
Identities = 36/135 (26%), Positives = 52/135 (37%), Gaps = 1/135 (0%)
Frame = +1
Query: 305 RYSAVRRQFGGNNGSLESQVIDYKTQQARLFPLLASAYAFRFVGEWLKWLY-TDVMKRLQ 363
RY R+QFG + + ++ Q +L +L + A VG L LY T M Q
Sbjct: 37 RYLKERKQFG-------APLAAFQINQQKLVQMLGNIQAMILVGWRLCKLYETGKMTVGQ 195
Query: 364 ANDFSTLPEAHACTAGLKSLTTSATSDGIEECRKLCGGHGYLCSSGLPELFAVYVPTCTY 423
A+ KS T + R+L GG+G L + + F P TY
Sbjct: 196 ASSG-------------KSWITLRARETAALGRELLGGNGILADFLVAKAFCDLEPIYTY 336
Query: 424 EGDNIVLLLQVARHL 438
EG + L R +
Sbjct: 337 EGTYDINTLVTGREI 381
>TC14538 similar to GB|AAH54382.1|32450414|BC054382 feminization 1 homolog a
{Mus musculus;}, partial (3%)
Length = 2631
Score = 28.5 bits (62), Expect = 3.6
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = -2
Query: 150 ETTATFDPKTDEFVIHSPTLTSSKWWPGGL 179
E +T P DE V HSP L+ + WP L
Sbjct: 677 EQRSTLGPSPDE*VDHSPHLSEATPWPSSL 588
>TC9632 similar to PIR|T47546|T47546 protein kinase-like - Arabidopsis
thaliana {Arabidopsis thaliana;}, partial (12%)
Length = 650
Score = 28.1 bits (61), Expect = 4.7
Identities = 16/48 (33%), Positives = 23/48 (47%)
Frame = +1
Query: 328 KTQQARLFPLLASAYAFRFVGEWLKWLYTDVMKRLQANDFSTLPEAHA 375
K + PLLAS G WLK L T ++R + S++P + A
Sbjct: 130 KVSDSLTAPLLASKRGISLEGCWLKSLKTVWVQRKEQQRLSSIPSSRA 273
>TC14281 similar to UP|Q9XHC6 (Q9XHC6) Cytochrome P450 monooxygenaseCYP93D1,
partial (97%)
Length = 1768
Score = 27.7 bits (60), Expect = 6.2
Identities = 12/41 (29%), Positives = 24/41 (58%)
Frame = +3
Query: 425 GDNIVLLLQVARHLMKVISRLGSGKKPVGTTAYMGRVEQLL 465
G+ +V+ ++ RH VIS + GKK G+ +G + +++
Sbjct: 555 GEAVVMRQELIRHTNNVISMMTMGKKSEGSKDEIGELRKVI 677
>AV766460
Length = 247
Score = 27.3 bits (59), Expect = 8.1
Identities = 14/46 (30%), Positives = 18/46 (38%)
Frame = -1
Query: 372 EAHACTAGLKSLTTSATSDGIEECRKLCGGHGYLCSSGLPELFAVY 417
E +AC G + EC C YLCS LP + V+
Sbjct: 211 EDNACACGFRKFLVENVG---RECSSFCFALLYLCSHFLPTILVVF 83
>BP073048
Length = 465
Score = 27.3 bits (59), Expect = 8.1
Identities = 19/78 (24%), Positives = 35/78 (44%), Gaps = 2/78 (2%)
Frame = -2
Query: 71 YAWKRIIELRLTEEEASMLRSFVDEPAFTDLHWGMFIPAIKGQGTEEQQQKWLPLAQRMQ 130
Y+++R I +R ++ RSF DEP T+ P ++ E Q L ++ +
Sbjct: 326 YSFRRTIWIRCSKSMIERFRSFQDEPESTNQE-----PKVRKFVHRESSQATLSISAYIF 162
Query: 131 I--IGCYAQTELGHGSNV 146
+ + Y ++G G V
Sbjct: 161 L*TVSIYLSIQVGRGRRV 108
>AV770176
Length = 188
Score = 27.3 bits (59), Expect = 8.1
Identities = 16/48 (33%), Positives = 22/48 (45%)
Frame = +1
Query: 163 VIHSPTLTSSKWWPGGLGKVSTHAVVYARLITDGRDHGVHGFIVQLRS 210
+I S T SKWW K++TH V + G G IV++ S
Sbjct: 22 LIVSDTGNVSKWWENSTWKMTTHLVALGKGQRMGYSSGEPKVIVEVPS 165
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.136 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,331,145
Number of Sequences: 28460
Number of extensions: 133450
Number of successful extensions: 579
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 574
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 576
length of query: 664
length of database: 4,897,600
effective HSP length: 96
effective length of query: 568
effective length of database: 2,165,440
effective search space: 1229969920
effective search space used: 1229969920
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC149079.11