BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149050.15 + phase: 0
(235 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14511 similar to AAS20952 (AAS20952) Calmodulin binding protei... 190 2e-49
TC8039 similar to AAS20952 (AAS20952) Calmodulin binding protein... 93 5e-20
BP067450 51 2e-07
TC12260 44 2e-05
TC13698 similar to GB|AAO63291.1|28950735|BT005227 At4g20000 {Ar... 44 3e-05
TC10343 similar to UP|Q9SZZ1 (Q9SZZ1) Fibrillarin-like protein (... 32 0.095
TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, ... 31 0.21
TC14866 similar to UP|Q8L5W2 (Q8L5W2) bZIP transcription factor ... 31 0.21
TC8330 weakly similar to GB|AAM61257.1|21536925|AY084696 calmodu... 31 0.21
TC9787 30 0.28
AV421229 30 0.28
AV411486 30 0.28
AI967639 30 0.36
TC11970 similar to UP|Q9FQZ7 (Q9FQZ7) Avr9/Cf-9 rapidly elicited... 30 0.47
AV408185 30 0.47
TC7956 similar to GB|AAM61419.1|21537078|AY084855 putaive DNA-bi... 29 0.62
TC9682 similar to UP|Q8VZC0 (Q8VZC0) DTDP-glucose 4-6-dehydratas... 29 0.62
TC16926 weakly similar to UP|Q9LHH2 (Q9LHH2) Ankyrin-like protei... 29 0.62
TC16353 weakly similar to UP|HQGT_ARATH (Q9M156) Probable hydroq... 29 0.81
AV425842 29 0.81
>TC14511 similar to AAS20952 (AAS20952) Calmodulin binding protein 25,
partial (13%)
Length = 617
Score = 190 bits (482), Expect = 2e-49
Identities = 104/167 (62%), Positives = 125/167 (74%), Gaps = 4/167 (2%)
Frame = +1
Query: 4 SENLAASIESWMYRPA-MTDTWLSDYISRDAETLTKALHKSLSSSSSPEDALSPFLNLIK 62
+ENLA SIE WMYRP+ + D+W +DYI+RDAETLTKAL KSLS+ + A+SP NL++
Sbjct: 127 AENLA-SIEPWMYRPSSVADSWFADYIARDAETLTKALQKSLSNDV--DAAISPLFNLVR 297
Query: 63 TDSATTTTT-TTPTVSSLSASDDSA--PKRQRVAAGKISKRKSRASKRSQTTFITADPAN 119
TD+A + TPTVSSLS SD + PKR RV+ G++SKRKSRASKRSQTTFITADPAN
Sbjct: 298 TDAAVSPALPATPTVSSLSGSDQDSQQPKRNRVSGGRVSKRKSRASKRSQTTFITADPAN 477
Query: 120 FRQMVQQVTGVRFGSGSNVSMAPLVKPEPHRAVGVNGGGRFTTGGGC 166
FRQMVQQ+TG RF MAP+V+PEP +A G GGR T G GC
Sbjct: 478 FRQMVQQITGARFTGSLPAPMAPVVRPEPLKAAG-GAGGRITVGAGC 615
>TC8039 similar to AAS20952 (AAS20952) Calmodulin binding protein 25,
partial (12%)
Length = 595
Score = 92.8 bits (229), Expect = 5e-20
Identities = 54/82 (65%), Positives = 62/82 (74%), Gaps = 3/82 (3%)
Frame = +2
Query: 157 GGRFTTGGGCL-PTLDTSAFLLQHHQQQQQTMVGPNS--DGPEMTGLGPLSFGQPIGEDA 213
GGR T G GCL PTLDTSAFLL HH Q VGP+S DGP ++G GP+SFGQ + +DA
Sbjct: 5 GGRITVGAGCLLPTLDTSAFLLDHH-HQNNAAVGPDSAGDGPGLSGPGPVSFGQ-LMDDA 178
Query: 214 AGYDFETFSSCFPTLESSWKVM 235
A +DFE+FSS FPTLE SWKVM
Sbjct: 179 ASFDFESFSS-FPTLE-SWKVM 238
>BP067450
Length = 372
Score = 51.2 bits (121), Expect = 2e-07
Identities = 27/43 (62%), Positives = 36/43 (82%)
Frame = -2
Query: 193 DGPEMTGLGPLSFGQPIGEDAAGYDFETFSSCFPTLESSWKVM 235
DGP ++G GP+SFGQ + +DAA +DFE+FSS FP+L+ SWKVM
Sbjct: 368 DGPGLSGPGPVSFGQ-LMDDAASFDFESFSS-FPSLD-SWKVM 249
>TC12260
Length = 320
Score = 44.3 bits (103), Expect = 2e-05
Identities = 37/120 (30%), Positives = 50/120 (40%), Gaps = 13/120 (10%)
Frame = +2
Query: 17 RPAMTDTWLSDYISRDAETLTKAL--HKSLSSSSSPEDALS--------PFLNLIKTDSA 66
RP + +W S S ++ H S S+ +D L P + A
Sbjct: 2 RPKPSPSWFSSSSSASCFFMSSECDPHPHNMSGSTSKDWLHFYQESSSFPTAGQVSVSEA 181
Query: 67 TTTTTTTPTVSSLSASDDSAPKRQRVAAGKISK---RKSRASKRSQTTFITADPANFRQM 123
TT TTTTPT Q G++SK R+SRAS+R+ TT + D NFR M
Sbjct: 182 TTVTTTTPT--------------QLNPDGRVSKPIRRRSRASRRTPTTLLNTDTTNFRAM 319
>TC13698 similar to GB|AAO63291.1|28950735|BT005227 At4g20000 {Arabidopsis
thaliana;}, partial (10%)
Length = 419
Score = 43.5 bits (101), Expect = 3e-05
Identities = 25/62 (40%), Positives = 39/62 (62%), Gaps = 4/62 (6%)
Frame = +2
Query: 72 TTPTVSS----LSASDDSAPKRQRVAAGKISKRKSRASKRSQTTFITADPANFRQMVQQV 127
+TPT+ S LS ++ +PK + K +R+SR+SK++ TT + A+ NFR +VQQ
Sbjct: 152 STPTIPSENTILSPTNSLSPKG---SLSKPIRRRSRSSKKTPTTLLNANTTNFRALVQQF 322
Query: 128 TG 129
TG
Sbjct: 323 TG 328
>TC10343 similar to UP|Q9SZZ1 (Q9SZZ1) Fibrillarin-like protein (Fibrillarin
2) (AtFib2), partial (85%)
Length = 1116
Score = 32.0 bits (71), Expect = 0.095
Identities = 16/44 (36%), Positives = 24/44 (54%)
Frame = -1
Query: 30 SRDAETLTKALHKSLSSSSSPEDALSPFLNLIKTDSATTTTTTT 73
S + T T L + S+SSS SP ++ + +TTTTTT+
Sbjct: 177 SSSSSTATSCLKRGTSTSSSSSSITSPSSSITPSPKSTTTTTTS 46
>TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, partial
(31%)
Length = 727
Score = 30.8 bits (68), Expect = 0.21
Identities = 18/57 (31%), Positives = 30/57 (52%)
Frame = -3
Query: 24 WLSDYISRDAETLTKALHKSLSSSSSPEDALSPFLNLIKTDSATTTTTTTPTVSSLS 80
W+ S A T + S SSSSSP + S + T S+T+++T++ + +S S
Sbjct: 347 WIGSSSSSSASASTSSSSSSSSSSSSPSSSSSSSSSSSSTSSSTSSSTSSYS*TSAS 177
>TC14866 similar to UP|Q8L5W2 (Q8L5W2) bZIP transcription factor ATB2,
partial (60%)
Length = 948
Score = 30.8 bits (68), Expect = 0.21
Identities = 15/42 (35%), Positives = 23/42 (54%)
Frame = +3
Query: 31 RDAETLTKALHKSLSSSSSPEDALSPFLNLIKTDSATTTTTT 72
++ ET L + S +L+ +N+IKT +A TTTTT
Sbjct: 213 QNVETENSILRAQMGELSQRLQSLNDIINVIKTSAAATTTTT 338
>TC8330 weakly similar to GB|AAM61257.1|21536925|AY084696 calmodulin-like
protein {Arabidopsis thaliana;} , partial (72%)
Length = 1174
Score = 30.8 bits (68), Expect = 0.21
Identities = 29/99 (29%), Positives = 49/99 (49%), Gaps = 7/99 (7%)
Frame = -1
Query: 33 AETLTKALHKSLSSSSS----PEDALSPFLNLIKTDSATTTTTTTPTVSSLSASDDSAPK 88
+ +++K + SLSS S P ++S ++ S +T+ + T T SSLS +AP+
Sbjct: 544 SRSVSKTSNASLSSVSGSDPGPTRSISASTLILPLPSPSTSRSITSTSSSLSC---NAPR 374
Query: 89 RQRVA---AGKISKRKSRASKRSQTTFITADPANFRQMV 124
R A A + SR+ +R TAD ++ R +V
Sbjct: 373 RVSTATRSARDTTPSLSRSMRRKAQRTSTADQSSGRTLV 257
>TC9787
Length = 716
Score = 30.4 bits (67), Expect = 0.28
Identities = 18/38 (47%), Positives = 26/38 (68%), Gaps = 1/38 (2%)
Frame = -3
Query: 54 LSPFLNLIKTDS-ATTTTTTTPTVSSLSASDDSAPKRQ 90
++P L+LIK S A TTT T TVSSLS+S+ + ++
Sbjct: 360 MTPALHLIKAISRAITTTGTRRTVSSLSSSNQGSSSKR 247
>AV421229
Length = 262
Score = 30.4 bits (67), Expect = 0.28
Identities = 12/20 (60%), Positives = 17/20 (85%)
Frame = +1
Query: 110 TTFITADPANFRQMVQQVTG 129
TTF+ AD +NFR +VQ++TG
Sbjct: 82 TTFVQADSSNFRAVVQKLTG 141
>AV411486
Length = 419
Score = 30.4 bits (67), Expect = 0.28
Identities = 22/50 (44%), Positives = 28/50 (56%), Gaps = 6/50 (12%)
Frame = +2
Query: 30 SRDAETLTKALHKSLSSS---SSPEDALS---PFLNLIKTDSATTTTTTT 73
S D +T K+LH SLS +SP S P ++ T +ATTTTTTT
Sbjct: 170 SSDHKTRQKSLHPSLSHGGFHASPTTKTSHHHPSMDGTTTATATTTTTTT 319
>AI967639
Length = 335
Score = 30.0 bits (66), Expect = 0.36
Identities = 23/73 (31%), Positives = 39/73 (52%)
Frame = -1
Query: 7 LAASIESWMYRPAMTDTWLSDYISRDAETLTKALHKSLSSSSSPEDALSPFLNLIKTDSA 66
L+ S+ S M + + + + S S + L + S SSSSSP + L L ++
Sbjct: 221 LSLSLSSLMLKSSSSSSSSS---SSSSSRLYSSP*SSSSSSSSPSELLEISLLCLRFLRY 51
Query: 67 TTTTTTTPTVSSL 79
+++ TT+PT+SSL
Sbjct: 50 SSSYTTSPTISSL 12
>TC11970 similar to UP|Q9FQZ7 (Q9FQZ7) Avr9/Cf-9 rapidly elicited protein
132, partial (24%)
Length = 789
Score = 29.6 bits (65), Expect = 0.47
Identities = 26/84 (30%), Positives = 34/84 (39%), Gaps = 3/84 (3%)
Frame = +1
Query: 22 DTWLSDYISRDAETLTKALHKS---LSSSSSPEDALSPFLNLIKTDSATTTTTTTPTVSS 78
D W + + + A H S L SSSS + P LI ++TTTPT SS
Sbjct: 85 DMWFQSHSTCPLCRTSVASHDSDNNLQSSSSDSSSTFPTNVLIWGTDTQISSTTTPT-SS 261
Query: 79 LSASDDSAPKRQRVAAGKISKRKS 102
L S P+ Q + S S
Sbjct: 262 LEGSTSQPPQAQLPCSSSSSSHTS 333
>AV408185
Length = 352
Score = 29.6 bits (65), Expect = 0.47
Identities = 20/60 (33%), Positives = 31/60 (51%), Gaps = 3/60 (5%)
Frame = +2
Query: 63 TDSATTTTTTTP---TVSSLSASDDSAPKRQRVAAGKISKRKSRASKRSQTTFITADPAN 119
T++AT + T++P + S SAS S+ A+ K S S +S RS +T + P N
Sbjct: 128 TNAATASATSSPGYPSSQSSSASAVSSATSCSAASSKASSSPSASSSRSSSTSSSKPPCN 307
>TC7956 similar to GB|AAM61419.1|21537078|AY084855 putaive DNA-binding
protein {Arabidopsis thaliana;} , partial (45%)
Length = 1257
Score = 29.3 bits (64), Expect = 0.62
Identities = 24/73 (32%), Positives = 38/73 (51%), Gaps = 1/73 (1%)
Frame = +1
Query: 13 SWMYRPAMTDTWLSDYISRDAETLTKALHKSLSSSSSPEDALSPFLNLIKTDSATTTTTT 72
SW ++P+ W++ + L S+SS SS L L+ T++ TTTT+T
Sbjct: 49 SWRWKPST*SGWMNTQPYKKLLQLA*TAW-SISSESSLLKPLLLQLHHHLTETTTTTTST 225
Query: 73 TP-TVSSLSASDD 84
TP + +SLS S +
Sbjct: 226 TPKSPTSLSPSSN 264
>TC9682 similar to UP|Q8VZC0 (Q8VZC0) DTDP-glucose 4-6-dehydratase-like
protein, partial (29%)
Length = 731
Score = 29.3 bits (64), Expect = 0.62
Identities = 21/48 (43%), Positives = 25/48 (51%), Gaps = 3/48 (6%)
Frame = +1
Query: 43 SLSSSSSPEDALSP---FLNLIKTDSATTTTTTTPTVSSLSASDDSAP 87
S+SSSSS E + +P N + SA TT TPT SS S S P
Sbjct: 328 SVSSSSSSESSSAPPSSSSNPLSPASALTTPHLTPTPSSPLPSQTSTP 471
>TC16926 weakly similar to UP|Q9LHH2 (Q9LHH2) Ankyrin-like protein, partial
(8%)
Length = 801
Score = 29.3 bits (64), Expect = 0.62
Identities = 25/91 (27%), Positives = 37/91 (40%), Gaps = 6/91 (6%)
Frame = +3
Query: 66 ATTTTTTTPTVSSLSASDDSAPKR------QRVAAGKISKRKSRASKRSQTTFITADPAN 119
++ + TTP+ S S ++D APK + + S S S + A PA
Sbjct: 258 SSRSAPTTPSSGSPSVTEDGAPKPKSPNGVKEKSPSSFSTIPSMNGTTSSASGAAAAPAP 437
Query: 120 FRQMVQQVTGVRFGSGSNVSMAPLVKPEPHR 150
R +G R S V +PL P+P R
Sbjct: 438 GRHRCCSSSGAR--RSSRVPGSPLYNPDPTR 524
>TC16353 weakly similar to UP|HQGT_ARATH (Q9M156) Probable hydroquinone
glucosyltransferase (Arbutin synthase) , partial (52%)
Length = 885
Score = 28.9 bits (63), Expect = 0.81
Identities = 27/100 (27%), Positives = 46/100 (46%)
Frame = +2
Query: 47 SSSPEDALSPFLNLIKTDSATTTTTTTPTVSSLSASDDSAPKRQRVAAGKISKRKSRASK 106
SSS AL+P + + T+++ P SLS+S A + +A+ + S +S++
Sbjct: 374 SSSTSSALTPSTSPENSAPPLTSSSLPPPPRSLSSSTSLAWTGRFIASSENSPNRSKSPA 553
Query: 107 RSQTTFITADPANFRQMVQQVTGVRFGSGSNVSMAPLVKP 146
S++T T FR + T SGS+ + KP
Sbjct: 554 PSRSTVQTFS-TRFRTGKMKPT-----SGSSTTQLDTAKP 655
>AV425842
Length = 404
Score = 28.9 bits (63), Expect = 0.81
Identities = 15/38 (39%), Positives = 23/38 (60%)
Frame = -1
Query: 85 SAPKRQRVAAGKISKRKSRASKRSQTTFITADPANFRQ 122
S+P+ + AAGK +RKSR+S+ +ADP R+
Sbjct: 293 SSPEIRTPAAGKFPRRKSRSSRICARGGFSADPQRRRR 180
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.311 0.125 0.359
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,936,377
Number of Sequences: 28460
Number of extensions: 50641
Number of successful extensions: 599
Number of sequences better than 10.0: 96
Number of HSP's better than 10.0 without gapping: 493
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 546
length of query: 235
length of database: 4,897,600
effective HSP length: 87
effective length of query: 148
effective length of database: 2,421,580
effective search space: 358393840
effective search space used: 358393840
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 54 (25.4 bits)
Medicago: description of AC149050.15