BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148996.5 + phase: 0
(196 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC19532 83 4e-17
AV413583 35 0.011
TC10727 weakly similar to UP|Q15287 (Q15287) RNA-binding protein... 32 0.073
TC12355 similar to UP|PR18_HUMAN (Q99633) Pre-mRNA splicing fact... 31 0.13
TC14206 similar to PIR|T49142|T49142 CCR4-associated factor 1-li... 31 0.13
TC19569 similar to UP|AAR11301 (AAR11301) Lectin-like receptor k... 31 0.16
TC11757 UP|Q91W27 (Q91W27) Tuberoinfundibular 39 residue protein... 30 0.21
TC12509 similar to UP|ACRO_HUMAN (P10323) Acrosin precursor , p... 30 0.28
TC20145 30 0.36
TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly pr... 30 0.36
TC12667 similar to PIR|D96525|D96525 protein T1N15.19 [imported]... 29 0.48
TC15267 similar to UP|EXTN_TOBAC (P13983) Extensin precursor (Ce... 29 0.48
TC8538 similar to UP|AAQ65130 (AAQ65130) At3g47300, partial (73%) 29 0.62
BP061908 29 0.62
TC10357 similar to UP|Q9T0K5 (Q9T0K5) Extensin-like protein, par... 29 0.62
TC15366 similar to GB|AAO63329.1|28950811|BT005265 At5g21920 {Ar... 29 0.62
CB829188 29 0.62
TC18261 similar to UP|WSC2_YEAST (P53832) Cell wall integrity an... 28 0.81
TC11304 similar to PIR|T04669|T04669 serine O-acetyltransferase ... 28 0.81
BI420843 28 0.81
>TC19532
Length = 497
Score = 82.8 bits (203), Expect = 4e-17
Identities = 39/51 (76%), Positives = 44/51 (85%)
Frame = +2
Query: 140 PERLGNLLMKTVLPGSTLYVEGNLETKVFSDPVTGLVRRVREVAVRRNGNL 190
PERLG L+M PGSTLYVEGNLE+KVFSDP TGLVRR+REV+VRRNG +
Sbjct: 2 PERLGTLVMTHATPGSTLYVEGNLESKVFSDPTTGLVRRIREVSVRRNGRV 154
>AV413583
Length = 429
Score = 34.7 bits (78), Expect = 0.011
Identities = 17/42 (40%), Positives = 21/42 (49%)
Frame = +1
Query: 14 RSLLSTPPPPLHHHRHIPLRFCTTTTSLSDYDDADSAAPSPS 55
RSLL PL H +P RFCT+ + S + SA PS
Sbjct: 91 RSLLLFTCHPLRRHHFLPFRFCTSPATTSSSSSSPSALSDPS 216
>TC10727 weakly similar to UP|Q15287 (Q15287) RNA-binding protein
(RNA-binding protein S1, serine-rich domain), partial
(15%)
Length = 932
Score = 32.0 bits (71), Expect = 0.073
Identities = 20/47 (42%), Positives = 25/47 (52%), Gaps = 2/47 (4%)
Frame = +3
Query: 18 STPPPPLH--HHRHIPLRFCTTTTSLSDYDDADSAAPSPSPEQTERT 62
S PPPP H HHRH P T++ L + + S +P PSP T T
Sbjct: 12 SPPPPPPHHNHHRHPP----PTSSPLPAHGASSSTSP-PSPAPTPTT 137
>TC12355 similar to UP|PR18_HUMAN (Q99633) Pre-mRNA splicing factor 18
(PRP18 homolog) (hPRP18), partial (8%)
Length = 563
Score = 31.2 bits (69), Expect = 0.13
Identities = 32/100 (32%), Positives = 45/100 (45%), Gaps = 3/100 (3%)
Frame = +1
Query: 20 PPPPLHHHRHIPL-RFCTTTTSLSDYDDADSA-APSPSPEQTERTFFDRPLENGLD-PGV 76
PPPP H P R TT++SL+ A SA + SPSP + T + + PG
Sbjct: 238 PPPPPTPPPHPPTSRTSTTSSSLNKKSSAASASSSSPSPSSAKTTTPGSTVSSTFSRPGS 417
Query: 77 YRAILVGKAGQKPLQKKLKSGTVVTLLSIGTGGIRNNRRP 116
R+I + G+ + + LLS G+G RRP
Sbjct: 418 LRSIAI*LRGRPTISSAI-------LLSSGSG-----RRP 501
>TC14206 similar to PIR|T49142|T49142 CCR4-associated factor 1-like protein
- Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(87%)
Length = 1151
Score = 31.2 bits (69), Expect = 0.13
Identities = 18/57 (31%), Positives = 24/57 (41%), Gaps = 12/57 (21%)
Frame = -3
Query: 18 STPPPPLHH------------HRHIPLRFCTTTTSLSDYDDADSAAPSPSPEQTERT 62
S PP P HH H+H + +T + + A SAA +PSP RT
Sbjct: 882 SEPPGPTHHTVPHRTASCTSHHKHFSRKTPSTISEIPPTHSAGSAASAPSPGSRNRT 712
>TC19569 similar to UP|AAR11301 (AAR11301) Lectin-like receptor kinase
1;1, partial (16%)
Length = 486
Score = 30.8 bits (68), Expect = 0.16
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = +2
Query: 20 PPPPLHHHRHIP 31
PPPP HHH H+P
Sbjct: 56 PPPPTHHHHHLP 91
Score = 27.3 bits (59), Expect = 1.8
Identities = 22/83 (26%), Positives = 28/83 (33%), Gaps = 17/83 (20%)
Frame = +1
Query: 9 TSTLYRSLLSTPPPPLHHHRHIPLRFCTTTT-----------------SLSDYDDADSAA 51
TS+ S S PPPP H P T+TT S S SA+
Sbjct: 43 TSSSSSSSYSPPPPPSQQPTHSPSTSPTSTTTPPPSPTKVTENPPTAPSTSTKSPTSSAS 222
Query: 52 PSPSPEQTERTFFDRPLENGLDP 74
PSP + +P + P
Sbjct: 223 EEPSPPNPSTSTTQQPKHSPTSP 291
>TC11757 UP|Q91W27 (Q91W27) Tuberoinfundibular 39 residue protein, partial
(10%)
Length = 715
Score = 30.4 bits (67), Expect = 0.21
Identities = 31/111 (27%), Positives = 47/111 (41%), Gaps = 4/111 (3%)
Frame = +2
Query: 69 ENGLDPGVYRAILV-GKAGQ-KPLQKKLKSGTVVTLLSIGTGGIRNNRRPLDHENPREYA 126
E G P VY+ +L GK G K L + K G + I N ++HEN +
Sbjct: 344 EGGFGP-VYKGVLKDGKVGAIKVLSAESKQGVKEFMTEI------NVISEIEHENLVQLY 502
Query: 127 NRCAVQWHRVTVYPERLGNLLMKTVLPG--STLYVEGNLETKVFSDPVTGL 175
C HR+ VY N L +T+L G S ++ + +++ GL
Sbjct: 503 GCCVEGNHRILVYNYHENNSLAQTLLGGGHSNIHFDWRTRSRICIGVARGL 655
>TC12509 similar to UP|ACRO_HUMAN (P10323) Acrosin precursor , partial (5%)
Length = 710
Score = 30.0 bits (66), Expect = 0.28
Identities = 18/44 (40%), Positives = 20/44 (44%)
Frame = +3
Query: 13 YRSLLSTPPPPLHHHRHIPLRFCTTTTSLSDYDDADSAAPSPSP 56
YRS + PPP +HH H L F T S PSPSP
Sbjct: 180 YRSQ-TRPPPTTNHHHHHHLPFTPLQTFNLPLLRCSSPPPSPSP 308
>TC20145
Length = 384
Score = 29.6 bits (65), Expect = 0.36
Identities = 13/28 (46%), Positives = 18/28 (63%)
Frame = -1
Query: 5 SISRTSTLYRSLLSTPPPPLHHHRHIPL 32
++SR +T SL++ PPPPL H PL
Sbjct: 165 TLSRAATTSFSLIAPPPPPLFHSAPPPL 82
>TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly protein
1-like protein 3, partial (81%)
Length = 1298
Score = 29.6 bits (65), Expect = 0.36
Identities = 14/34 (41%), Positives = 15/34 (43%)
Frame = -1
Query: 21 PPPLHHHRHIPLRFCTTTTSLSDYDDADSAAPSP 54
P PLHHH H+ L L Y SA P P
Sbjct: 839 PLPLHHHHHLHLHLHHHLHHLLQYPQNHSAQPPP 738
>TC12667 similar to PIR|D96525|D96525 protein T1N15.19 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(10%)
Length = 498
Score = 29.3 bits (64), Expect = 0.48
Identities = 26/74 (35%), Positives = 33/74 (44%), Gaps = 9/74 (12%)
Frame = +1
Query: 2 AKFSISRTSTLYRSLLSTPP----PPLHHHRHIPLRFCTTTTSLSDYDDADSAAPS---- 53
A F SRT T LS P PPLH HR R C + T+ + ++ +A S
Sbjct: 145 ALFRTSRTITTNSPFLSFKPILPFPPLHFHR----RTCFSATATTTTEEITPSAASSDQL 312
Query: 54 -PSPEQTERTFFDR 66
P PE +F DR
Sbjct: 313 HPWPEWV--SFIDR 348
>TC15267 similar to UP|EXTN_TOBAC (P13983) Extensin precursor (Cell wall
hydroxyproline-rich glycoprotein), partial (6%)
Length = 1003
Score = 29.3 bits (64), Expect = 0.48
Identities = 12/22 (54%), Positives = 13/22 (58%)
Frame = +2
Query: 21 PPPLHHHRHIPLRFCTTTTSLS 42
PPP HHH H TTT+ LS
Sbjct: 206 PPPPHHHHHQATAQTTTTSQLS 271
>TC8538 similar to UP|AAQ65130 (AAQ65130) At3g47300, partial (73%)
Length = 1135
Score = 28.9 bits (63), Expect = 0.62
Identities = 15/55 (27%), Positives = 20/55 (36%)
Frame = +2
Query: 20 PPPPLHHHRHIPLRFCTTTTSLSDYDDADSAAPSPSPEQTERTFFDRPLENGLDP 74
PP P HHHRH P+ P P P + F ++G+ P
Sbjct: 167 PPKPTHHHRHHPI-------------------PQPQPHLQKPLDFPAQKQSGIGP 274
>BP061908
Length = 472
Score = 28.9 bits (63), Expect = 0.62
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = -2
Query: 18 STPPPPLHHHRHIPLRFCTTTTSLS 42
S P PP+HHH P TT+LS
Sbjct: 465 SPPTPPVHHHHRRPPAAARRTTTLS 391
>TC10357 similar to UP|Q9T0K5 (Q9T0K5) Extensin-like protein, partial (3%)
Length = 836
Score = 28.9 bits (63), Expect = 0.62
Identities = 19/45 (42%), Positives = 20/45 (44%), Gaps = 3/45 (6%)
Frame = +2
Query: 18 STPPPP---LHHHRHIPLRFCTTTTSLSDYDDADSAAPSPSPEQT 59
S+PPPP LHHH H TTTT L S SP T
Sbjct: 227 SSPPPPSSPLHHHHHP----STTTTVLLRPGTTRSVTTKNSPSAT 349
>TC15366 similar to GB|AAO63329.1|28950811|BT005265 At5g21920 {Arabidopsis
thaliana;}, partial (23%)
Length = 568
Score = 28.9 bits (63), Expect = 0.62
Identities = 15/41 (36%), Positives = 19/41 (45%)
Frame = +3
Query: 24 LHHHRHIPLRFCTTTTSLSDYDDADSAAPSPSPEQTERTFF 64
L HH H P + TS + +S PSP P+ T FF
Sbjct: 159 LKHHSHSPFSNPQSPTSPLSFLPLNSTNPSPPPQITVSDFF 281
>CB829188
Length = 236
Score = 28.9 bits (63), Expect = 0.62
Identities = 18/56 (32%), Positives = 20/56 (35%), Gaps = 1/56 (1%)
Frame = +2
Query: 20 PPPPLHHHRHIPLRFCTTTTSLSDYDDAD-SAAPSPSPEQTERTFFDRPLENGLDP 74
PPPPLH P T S + + S P PSP T R P P
Sbjct: 47 PPPPLHSSSSHPPSLSTLNPSPPSHSHPNPSPKPLPSPSTTTRATLS*PASTTATP 214
>TC18261 similar to UP|WSC2_YEAST (P53832) Cell wall integrity and stress
response component 2 precursor, partial (5%)
Length = 538
Score = 28.5 bits (62), Expect = 0.81
Identities = 18/46 (39%), Positives = 22/46 (47%), Gaps = 4/46 (8%)
Frame = +3
Query: 21 PPPLHHHRHIPLRFCTTTTSLSDYDDADSAA----PSPSPEQTERT 62
PPPL + L TT+TS S + SAA SPSP + T
Sbjct: 216 PPPLQPPTSLSLMTSTTSTSSSSPNSRSSAASASSSSPSPSSVKTT 353
>TC11304 similar to PIR|T04669|T04669 serine O-acetyltransferase F8D20.150
- Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (24%)
Length = 775
Score = 28.5 bits (62), Expect = 0.81
Identities = 17/35 (48%), Positives = 18/35 (50%)
Frame = +1
Query: 8 RTSTLYRSLLSTPPPPLHHHRHIPLRFCTTTTSLS 42
RT+TL PPPP HHH H TTT LS
Sbjct: 238 RTTTLL------PPPPHHHHHH------PTTTILS 306
>BI420843
Length = 377
Score = 28.5 bits (62), Expect = 0.81
Identities = 18/47 (38%), Positives = 22/47 (46%), Gaps = 1/47 (2%)
Frame = -3
Query: 18 STPPPPLHHHRHIPLRFCTTTTSLSDYDDADSAAPSPSPE-QTERTF 63
S+ PPP HH R T +L + DS P+P P QT R F
Sbjct: 267 SSEPPPEHHTR--------TPAALVRHRRGDSPPPAPPPSVQTRRCF 151
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.134 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,674,228
Number of Sequences: 28460
Number of extensions: 63811
Number of successful extensions: 1050
Number of sequences better than 10.0: 120
Number of HSP's better than 10.0 without gapping: 938
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1004
length of query: 196
length of database: 4,897,600
effective HSP length: 85
effective length of query: 111
effective length of database: 2,478,500
effective search space: 275113500
effective search space used: 275113500
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)
Medicago: description of AC148996.5