BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148996.16 - phase: 0 /pseudo
(332 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18399 216 3e-57
TC11756 weakly similar to GB|AAH01494.1|16306637|BC001494 U5 snR... 41 2e-04
AV767790 32 0.19
TC9693 similar to UP|COPP_MOUSE (O55029) Coatomer beta' subunit ... 31 0.33
TC13023 similar to UP|Q8L830 (Q8L830) WD40-repeat protein (Fragm... 29 1.3
TC10816 homologue to UP|CAE45585 (CAE45585) Coatomer alpha subun... 28 1.6
TC17581 similar to GB|AAP81802.1|32441254|BT009684 At1g74380 {Ar... 28 2.1
TC12572 similar to PIR|T46032|T46032 WD-40 repeat regulatory pro... 28 2.1
BP052883 28 2.8
TC14227 homologue to UP|Q9LLR4 (Q9LLR4) Sec61p, partial (53%) 27 3.7
TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%) 27 4.8
BG662118 27 4.8
AV410177 27 6.2
BG662335 26 8.1
>TC18399
Length = 542
Score = 216 bits (551), Expect = 3e-57
Identities = 103/121 (85%), Positives = 114/121 (94%)
Frame = +1
Query: 1 MEINLGKLAFDIDFHPSENLVASGLIDGDLHLYRYSSDNTNSDPVRVLEVHAHTESCRAA 60
MEINLGKL FDIDFHPS+NLVA+GLI GD H+YRYSSD+ ++DPVR+LEVHAHTESCRAA
Sbjct: 145 MEINLGKLGFDIDFHPSDNLVAAGLISGDFHIYRYSSDSNDNDPVRLLEVHAHTESCRAA 324
Query: 61 RFINGGRAVLTGSPDFSILATDVETGSTIARLDNAHEAAVNRLINLTESTVASGDDDGCI 120
RFINGGR +LTGSPD SILATDVETGSTI R+DNAHEAA+NRLINLTESTVASGDD+GCI
Sbjct: 325 RFINGGRVLLTGSPDCSILATDVETGSTITRIDNAHEAAINRLINLTESTVASGDDEGCI 504
Query: 121 K 121
K
Sbjct: 505 K 507
>TC11756 weakly similar to GB|AAH01494.1|16306637|BC001494 U5 snRNP-specific
40 kDa protein (hPrp8-binding) {Homo sapiens;} , partial
(43%)
Length = 719
Score = 41.2 bits (95), Expect = 2e-04
Identities = 28/114 (24%), Positives = 52/114 (45%), Gaps = 2/114 (1%)
Frame = +1
Query: 10 FDIDFHPSENLVASGLIDGDLHLYRYSSDNTNSDPVRVLEVHAHTESCRAARFINGGRAV 69
+ + F+P+ ++ASG D ++ L+ N + + + + H + + G +
Sbjct: 316 YTMKFNPAGTVIASGSHDREIFLW-----NVHGECKNFMVLKGHKNAVLDLHWTTDGTQI 480
Query: 70 LTGSPDFSILATDVETGSTIARLDNAHEAAVNRLINLTES--TVASGDDDGCIK 121
++ SPD ++ DVETG + ++ H + VN V SG DDG K
Sbjct: 481 VSASPDKTVRVWDVETGKQVKKMVE-HLSYVNSCCPSRRGPPLVVSGSDDGTAK 639
>AV767790
Length = 617
Score = 31.6 bits (70), Expect = 0.19
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +3
Query: 248 FSHDRKFLGSIGHDQMLKLWDLDNI 272
FS D K L S GHDQ + LW+++ +
Sbjct: 498 FSSDGKLLASAGHDQKVVLWNMETL 572
>TC9693 similar to UP|COPP_MOUSE (O55029) Coatomer beta' subunit
(Beta'-coat protein) (Beta'-COP) (p102), partial (20%)
Length = 743
Score = 30.8 bits (68), Expect = 0.33
Identities = 28/111 (25%), Positives = 51/111 (45%), Gaps = 1/111 (0%)
Frame = +1
Query: 12 IDFHPSENLVASGLIDGDLHLYRYSSDNTNSDPVRVLEVHAHTESCRAARFINGGRAVLT 71
+D HP+E + + L G + ++ Y + T + V E+ R+A+FI + V+
Sbjct: 268 VDLHPTEPWILASLYSGTVCIWNYQT-QTMAKSFEVTEL-----PVRSAKFIARKQWVVA 429
Query: 72 GSPDFSILATDVETGSTIARLDNAHEAAVNRL-INLTESTVASGDDDGCIK 121
G+ D I + T + ++ AH + + ++ T V S DD IK
Sbjct: 430 GADDMFIRVYNYNTMDKV-KVFEAHTDYIRCVAVHPTLPYVLSSSDDMLIK 579
>TC13023 similar to UP|Q8L830 (Q8L830) WD40-repeat protein (Fragment),
partial (18%)
Length = 591
Score = 28.9 bits (63), Expect = 1.3
Identities = 14/44 (31%), Positives = 20/44 (44%)
Frame = +1
Query: 229 NRIIEPIAEHSEYPVERLAFSHDRKFLGSIGHDQMLKLWDLDNI 272
N I + V LA S D L S GH + +++WDL +
Sbjct: 250 NASIRSTLQGDSESVTALALSPDDNLLFSSGHSRQIRVWDLSTL 381
>TC10816 homologue to UP|CAE45585 (CAE45585) Coatomer alpha subunit-like
protein, partial (20%)
Length = 979
Score = 28.5 bits (62), Expect = 1.6
Identities = 16/57 (28%), Positives = 27/57 (47%)
Frame = +1
Query: 212 IITGSENGMINLVGILPNRIIEPIAEHSEYPVERLAFSHDRKFLGSIGHDQMLKLWD 268
I+ +G+I L +I+ EH + PV + F H + S G D +K+W+
Sbjct: 310 ILASLHSGVIQLWDYRMGTLIDKFDEH-DGPVRGVHFHHSQPLFVSGGDDYKIKVWN 477
Score = 26.2 bits (56), Expect = 8.1
Identities = 24/108 (22%), Positives = 49/108 (45%), Gaps = 2/108 (1%)
Frame = +1
Query: 213 ITGSENGMINLVGILPNRIIEPIAEHSEYPVERLAFSHDRKFLGSIGHDQMLKLWDLDNI 272
++G ++ I + +R + + H +Y + + F H+ ++ S DQ +++W+ +
Sbjct: 439 VSGGDDYKIKVWNYKLHRCLFTLLGHLDY-IRTVQFHHESPWIVSASDDQTIRIWNWQSR 615
Query: 273 LQGSRSTQRNETGVVANDGDSDD-GDEMDVDNSASKFSKGN-KRKNAS 318
S T N + A +D +D + + G+ KRKNAS
Sbjct: 616 TCISVLTGHNHYVMCALFHPREDLVVSASLDQTVRVWDIGSLKRKNAS 759
>TC17581 similar to GB|AAP81802.1|32441254|BT009684 At1g74380 {Arabidopsis
thaliana;}, partial (35%)
Length = 804
Score = 28.1 bits (61), Expect = 2.1
Identities = 18/54 (33%), Positives = 28/54 (51%), Gaps = 2/54 (3%)
Frame = +3
Query: 233 EPIAEHSEYPVERLAFSHDRKFLGSIGHDQMLKLWDLDN--ILQGSRSTQRNET 284
+P + +YPVER S +R F + +Q+LKL+ + +L RNET
Sbjct: 306 KPCGSYGDYPVERCLSSMERAF--NFADNQVLKLYGFRHRGLLSPKIKRIRNET 461
>TC12572 similar to PIR|T46032|T46032 WD-40 repeat regulatory protein tup1
homolog - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (51%)
Length = 770
Score = 28.1 bits (61), Expect = 2.1
Identities = 16/63 (25%), Positives = 33/63 (51%), Gaps = 7/63 (11%)
Frame = +3
Query: 212 IITGSENGMINLVGILPNRIIEPIAEHSE-------YPVERLAFSHDRKFLGSIGHDQML 264
++ GSE+ I L + +I++ + HS+ +P E + S G++G+D+ +
Sbjct: 348 VVGGSEDHGIYLWELQTRKIVQKLEGHSDTVVSVSCHPTENMIAS------GALGNDKTV 509
Query: 265 KLW 267
K+W
Sbjct: 510 KIW 518
>BP052883
Length = 458
Score = 27.7 bits (60), Expect = 2.8
Identities = 17/57 (29%), Positives = 30/57 (51%)
Frame = -1
Query: 62 FINGGRAVLTGSPDFSILATDVETGSTIARLDNAHEAAVNRLINLTESTVASGDDDG 118
F++G ++TGSP F + TD G + +++ H + ++L E GD+DG
Sbjct: 401 FVSGSVLLVTGSPXFGVYGTDFGFGRPV-KVEMVHSF---KGVSLAE----CGDEDG 255
>TC14227 homologue to UP|Q9LLR4 (Q9LLR4) Sec61p, partial (53%)
Length = 880
Score = 27.3 bits (59), Expect = 3.7
Identities = 10/28 (35%), Positives = 18/28 (63%)
Frame = -3
Query: 237 EHSEYPVERLAFSHDRKFLGSIGHDQML 264
EH + P+++L+ + L SIGHD ++
Sbjct: 536 EHRQQPLQQLSIYQEASVLHSIGHDPLV 453
>TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%)
Length = 1008
Score = 26.9 bits (58), Expect = 4.8
Identities = 18/70 (25%), Positives = 33/70 (46%), Gaps = 9/70 (12%)
Frame = -2
Query: 268 DLDNILQGSRSTQRNET---------GVVANDGDSDDGDEMDVDNSASKFSKGNKRKNAS 318
DL +L + +T + ET DG+ D+GDE D+ F +G + ++
Sbjct: 704 DLCGLLLINMATTKGETKSQSEDKQHDAEGEDGNDDEGDE--DDDGDGPFGEGEEDLSSE 531
Query: 319 NGHAVGDSNN 328
+G G+++N
Sbjct: 530 DGAGYGNNSN 501
>BG662118
Length = 421
Score = 26.9 bits (58), Expect = 4.8
Identities = 16/59 (27%), Positives = 28/59 (47%)
Frame = +3
Query: 212 IITGSENGMINLVGILPNRIIEPIAEHSEYPVERLAFSHDRKFLGSIGHDQMLKLWDLD 270
I G E+ I++ + + + + H + + LAFS + L S G D L +W +D
Sbjct: 165 IAIGMEDSTIHIYNVRVDEVKSKLKGHHKR-ITGLAFSTNLNILVSSGADAQLCVWSID 338
>AV410177
Length = 418
Score = 26.6 bits (57), Expect = 6.2
Identities = 16/49 (32%), Positives = 31/49 (62%), Gaps = 4/49 (8%)
Frame = -2
Query: 202 LQTLLIPC*RIITGSENGMINLVGILPNRII----EPIAEHSEYPVERL 246
+QTLL IITG + ++ +G +P+R+I + I +H++ P+++L
Sbjct: 255 IQTLLPKS--IITGLTSNLVLSLGGVPSRLIFNIQQQIHQHTKQPLDKL 115
>BG662335
Length = 336
Score = 26.2 bits (56), Expect = 8.1
Identities = 14/36 (38%), Positives = 21/36 (57%), Gaps = 3/36 (8%)
Frame = -3
Query: 289 NDGDSDDGDE---MDVDNSASKFSKGNKRKNASNGH 321
+DG DDGDE +D D +AS + + RK+ + H
Sbjct: 334 DDGGDDDGDEDVDVDADATASP*AASSCRKHPPHPH 227
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.326 0.141 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,044,601
Number of Sequences: 28460
Number of extensions: 61365
Number of successful extensions: 404
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 396
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 403
length of query: 332
length of database: 4,897,600
effective HSP length: 91
effective length of query: 241
effective length of database: 2,307,740
effective search space: 556165340
effective search space used: 556165340
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 55 (25.8 bits)
Medicago: description of AC148996.16