BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148996.15 + phase: 0
(153 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV409338 33 0.022
TC9999 similar to UP|Q9FMU0 (Q9FMU0) Similarity to ABC transport... 28 0.54
TC14627 27 1.2
TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, ... 27 1.6
AV409840 27 2.0
BP047590 26 2.7
AV778286 26 2.7
TC8686 similar to UP|O23724 (O23724) GAI protein, partial (22%) 26 3.5
BI418821 26 3.5
TC18472 26 3.5
TC8093 homologue to UP|EFTU_TOBAC (P41342) Elongation factor Tu,... 26 3.5
TC15826 similar to UP|O48700 (O48700) F3I6.27 protein, partial (8%) 26 3.5
TC10962 similar to UP|Q9SV20 (Q9SV20) Beta-COP-like protein, par... 26 3.5
AU251812 25 4.5
AW719706 25 4.5
BI417326 25 4.5
AV409645 25 5.9
TC9060 homologue to UP|Q7VAN1 (Q7VAN1) SAM-dependent methyltrans... 25 5.9
TC19185 similar to UP|Q9LJF4 (Q9LJF4) Emb|CAB87904.1, partial (14%) 25 7.7
TC13149 25 7.7
>AV409338
Length = 210
Score = 33.1 bits (74), Expect = 0.022
Identities = 12/29 (41%), Positives = 23/29 (78%)
Frame = -2
Query: 75 SNISNKALSQFGKKKQREEREKEGWGNGG 103
SN+ N ++FGK ++ E++++EG+G+GG
Sbjct: 98 SNLRNVRETRFGKIQEEEKKDREGYGDGG 12
>TC9999 similar to UP|Q9FMU0 (Q9FMU0) Similarity to ABC transporter,
partial (43%)
Length = 656
Score = 28.5 bits (62), Expect = 0.54
Identities = 14/38 (36%), Positives = 22/38 (57%), Gaps = 2/38 (5%)
Frame = -1
Query: 70 PKKLLSNISNK--ALSQFGKKKQREEREKEGWGNGGVW 105
P+ L++NISN +S +K++ E E W +GG W
Sbjct: 302 PRSLIANISNFK*GVSAVDSEKRKRWWELERWWSGGKW 189
>TC14627
Length = 1237
Score = 27.3 bits (59), Expect = 1.2
Identities = 16/33 (48%), Positives = 21/33 (63%)
Frame = +1
Query: 39 EKAKSSSTTKPIRDEEWRFDLKTPPKSPMAKPK 71
E+A SSSTT R+ L T PK+P++KPK
Sbjct: 61 EQALSSSTTTTFRN------LLTFPKTPISKPK 141
>TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, partial
(31%)
Length = 727
Score = 26.9 bits (58), Expect = 1.6
Identities = 22/103 (21%), Positives = 39/103 (37%), Gaps = 14/103 (13%)
Frame = +2
Query: 11 NRHHSNGGFFVTISAILALLSRKANRLK------EKAKSSSTTKPIRDEE--------WR 56
NRH ++ +S + L K ++ K SS+ P+ WR
Sbjct: 2 NRHEASPNLKTLVSTLTHSLHSKNHQTKPWLTNPSPRSESSSPNPLNRRSYRSSCRTSWR 181
Query: 57 FDLKTPPKSPMAKPKKLLSNISNKALSQFGKKKQREEREKEGW 99
K+ KS KK + + KKK++++++K W
Sbjct: 182 LRFKSTKKSKKK*RKKWRRKKKRRKRKRRVKKKKKKKKKKRKW 310
>AV409840
Length = 417
Score = 26.6 bits (57), Expect = 2.0
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = -1
Query: 40 KAKSSSTTKPIRDEEWRFDLKTPPKSPMAKPKK 72
+ S S T+P R L+ PP+SP +K KK
Sbjct: 228 RGSSGSWTRPNRRPPMTPGLRDPPQSPPSKKKK 130
>BP047590
Length = 513
Score = 26.2 bits (56), Expect = 2.7
Identities = 19/56 (33%), Positives = 26/56 (45%)
Frame = +2
Query: 90 QREEREKEGWGNGGVWQKEILMGGKCEPLDFSGVIYYDINGKQTREVPIRSPRASP 145
Q+E++ KEGWG+ ++ E P SGV NG T + RASP
Sbjct: 29 QKEKKRKEGWGSKIIYNTENEYYS*PHP*FISGVAETAENGIST--LSFTRHRASP 190
>AV778286
Length = 554
Score = 26.2 bits (56), Expect = 2.7
Identities = 21/75 (28%), Positives = 28/75 (37%)
Frame = +3
Query: 42 KSSSTTKPIRDEEWRFDLKTPPKSPMAKPKKLLSNISNKALSQFGKKKQREEREKEGWGN 101
K + T PI ++ TP K P KP S S KK Q
Sbjct: 312 KPRAATSPISSKQPTTTPATPSKFPTTKPVAP-SRWPTTTSSSVAKKHQ----------- 455
Query: 102 GGVWQKEILMGGKCE 116
VWQ+ +L+ G C+
Sbjct: 456 --VWQQALLLQGHCQ 494
>TC8686 similar to UP|O23724 (O23724) GAI protein, partial (22%)
Length = 786
Score = 25.8 bits (55), Expect = 3.5
Identities = 12/26 (46%), Positives = 17/26 (65%), Gaps = 4/26 (15%)
Frame = +1
Query: 87 KKKQREEREK---EGW-GNGGVWQKE 108
KKK + E E+ + W G GGVW++E
Sbjct: 94 KKKMKREHEQVSGKSWMGGGGVWEEE 171
>BI418821
Length = 614
Score = 25.8 bits (55), Expect = 3.5
Identities = 21/77 (27%), Positives = 30/77 (38%), Gaps = 16/77 (20%)
Frame = -3
Query: 37 LKEKAKSSSTTKPIRD--------EEWRFDLKTPPKSPMAKPKKLL--------SNISNK 80
L A +ST P R E+W +PP S + KPK L N+S+
Sbjct: 246 LSPVAMENSTRSPSRRVR*PSDLMEDWWTKRSSPPSSGLMKPKPLTLLNH*TTPLNLSSA 67
Query: 81 ALSQFGKKKQREEREKE 97
+FG + R + E
Sbjct: 66 IGLRFGNPRHRHKWRNE 16
>TC18472
Length = 642
Score = 25.8 bits (55), Expect = 3.5
Identities = 24/83 (28%), Positives = 40/83 (47%), Gaps = 9/83 (10%)
Frame = -2
Query: 68 AKPKKLLSNISN----KALSQFGKK--KQREEREKEG-WGNGGV--WQKEILMGGKCEPL 118
A+PK + N K + KK K+ +ER +EG W N ++ I K +
Sbjct: 482 ARPKYMKKNSRKTEHIKVTTYAAKKIIKRSKERTQEGSWQNNSSKPLRRHIPAAPKLQRA 303
Query: 119 DFSGVIYYDINGKQTREVPIRSP 141
+ +++ + G+QTR +PI SP
Sbjct: 302 ANNRALHHSV-GQQTRVIPISSP 237
>TC8093 homologue to UP|EFTU_TOBAC (P41342) Elongation factor Tu,
chloroplast precursor (EF-Tu), partial (58%)
Length = 1146
Score = 25.8 bits (55), Expect = 3.5
Identities = 10/23 (43%), Positives = 15/23 (64%)
Frame = -3
Query: 88 KKQREEREKEGWGNGGVWQKEIL 110
K+ ER+KE GG+W+K+ L
Sbjct: 178 KRSESERKKEKGVGGGIWRKKKL 110
>TC15826 similar to UP|O48700 (O48700) F3I6.27 protein, partial (8%)
Length = 676
Score = 25.8 bits (55), Expect = 3.5
Identities = 18/54 (33%), Positives = 24/54 (44%), Gaps = 2/54 (3%)
Frame = -3
Query: 63 PKSPMAKPKK--LLSNISNKALSQFGKKKQREEREKEGWGNGGVWQKEILMGGK 114
P SP P+ I++K L+ GKKK G W++ IL GGK
Sbjct: 587 PHSPKETPE*T*FTFTITSKILTLSGKKK----------GKDNTWERLILQGGK 456
>TC10962 similar to UP|Q9SV20 (Q9SV20) Beta-COP-like protein, partial (36%)
Length = 1068
Score = 25.8 bits (55), Expect = 3.5
Identities = 17/40 (42%), Positives = 21/40 (52%), Gaps = 5/40 (12%)
Frame = -3
Query: 23 ISAILALLSRKANRLKEKAKSSS---TTKPIRD--EEWRF 57
+SA LL R+ N LKE SSS T P+R W+F
Sbjct: 508 LSACTQLLPREKNHLKESVISSSQ*KTANPVRG*VVTWQF 389
>AU251812
Length = 244
Score = 25.4 bits (54), Expect = 4.5
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = -2
Query: 84 QFGKKKQREEREKEGWGNGGVWQKE 108
+ GK+K+ + G NGG+W+KE
Sbjct: 105 EMGKRKR*QRWFD*GRSNGGIWKKE 31
>AW719706
Length = 432
Score = 25.4 bits (54), Expect = 4.5
Identities = 20/76 (26%), Positives = 35/76 (45%), Gaps = 7/76 (9%)
Frame = -3
Query: 48 KPIRDEEWRFDLKTPPKSPMAKPKKLLSNISNKALSQFGKKKQREEREKEGWGNG-GVWQ 106
+ IR W ++K P + L + + A + FG +REER +EG G + +
Sbjct: 403 RSIRRARWSSNIKIKPYAD------LFNLLGIHAGNAFGGLPEREERIREGGGAARAIGE 242
Query: 107 KEILM------GGKCE 116
+++ GG+CE
Sbjct: 241 GRVVVVVVGV*GGECE 194
>BI417326
Length = 478
Score = 25.4 bits (54), Expect = 4.5
Identities = 13/33 (39%), Positives = 16/33 (48%), Gaps = 1/33 (3%)
Frame = +3
Query: 42 KSSSTTKP-IRDEEWRFDLKTPPKSPMAKPKKL 73
+SS+ P + WR KTPP SP P L
Sbjct: 51 RSSTVASPSAANPHWRRTTKTPPPSPPPPPTTL 149
>AV409645
Length = 414
Score = 25.0 bits (53), Expect = 5.9
Identities = 16/54 (29%), Positives = 26/54 (47%), Gaps = 1/54 (1%)
Frame = -1
Query: 55 WRFDLKTPPKSPMAKPKKLLSNISNKA-LSQFGKKKQREEREKEGWGNGGVWQK 107
WR L P + P+ ++ +++ L +FGK RE +E N GVW +
Sbjct: 267 WRKLLLLP*RRMENSPEGVVGIVNSPIRLERFGKGN*RESSAEEMKKNHGVWSR 106
>TC9060 homologue to UP|Q7VAN1 (Q7VAN1) SAM-dependent methyltransferase,
partial (5%)
Length = 595
Score = 25.0 bits (53), Expect = 5.9
Identities = 8/24 (33%), Positives = 17/24 (70%)
Frame = +3
Query: 2 VKITSPESPNRHHSNGGFFVTISA 25
++I +P +PN+H+ FF+T+ +
Sbjct: 282 LRIKNPTNPNQHNGIENFFLTLQS 353
>TC19185 similar to UP|Q9LJF4 (Q9LJF4) Emb|CAB87904.1, partial (14%)
Length = 533
Score = 24.6 bits (52), Expect = 7.7
Identities = 11/36 (30%), Positives = 20/36 (55%)
Frame = +1
Query: 73 LLSNISNKALSQFGKKKQREEREKEGWGNGGVWQKE 108
+L +++ + + GK Q+ + GWGN VW +E
Sbjct: 49 ILGDLAGEGKVEAGKCPQKFLLWQPGWGNQIVWVEE 156
>TC13149
Length = 521
Score = 24.6 bits (52), Expect = 7.7
Identities = 12/31 (38%), Positives = 15/31 (47%)
Frame = +1
Query: 98 GWGNGGVWQKEILMGGKCEPLDFSGVIYYDI 128
GWG GG W+K CE L+ +Y I
Sbjct: 421 GWGGGGSWKK---YNYGCEVLEPETQVYMTI 504
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.313 0.133 0.391
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,825,034
Number of Sequences: 28460
Number of extensions: 35925
Number of successful extensions: 242
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 242
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 242
length of query: 153
length of database: 4,897,600
effective HSP length: 82
effective length of query: 71
effective length of database: 2,563,880
effective search space: 182035480
effective search space used: 182035480
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 51 (24.3 bits)
Medicago: description of AC148996.15